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Assessment of a natural dietary extract, titrated in phenylpropanoid glycosides, on blood parameters and plasma oxidative status in intensively reared Italian hares (Lepus corsicanus)

Published online by Cambridge University Press:  14 January 2011

M. Palazzo ,
F. Vizzarri ,
M. Cinone ,
C. Corino  and
D. Casamassima
M. Palazzo
Affiliation:
Department of Animal Plant and Environmental Science, Faculty of Agriculture, Università degli Studi del Molise, Via De Sanctis, snc, 86100 Campobasso, Italy
F. Vizzarri
Affiliation:
Department of Animal Plant and Environmental Science, Faculty of Agriculture, Università degli Studi del Molise, Via De Sanctis, snc, 86100 Campobasso, Italy
M. Cinone
Affiliation:
Department of Animal Production, Faculty of Veterinary Medicine, Università degli Studi di Bari, Strada provinciale per Casamassima km 3–70010 Valenzano Bari, Italy
C. Corino
Affiliation:
Department of Veterinary Sciences and Technologies for Food Security, Faculty of Veterinary Medicine, Università degli Studi di Milano, Via Celoria, 10–20133 Milano, Italy
D. Casamassima*
Affiliation:
Department of Animal Plant and Environmental Science, Faculty of Agriculture, Università degli Studi del Molise, Via De Sanctis, snc, 86100 Campobasso, Italy
*
†E-mail: [email protected]
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Abstract

Two different doses of a natural extract titrated in phenylpropanoid glycosides (PPGs) were evaluated for their effect on blood parameters and plasma oxidative status in pairs of intensively reared Italian hares. The study lasted 210 days, during which 45 couples of hares were divided into three homogeneous groups. A control group (CON) was fed a control diet while the two experimental groups were fed a diet supplemented with 1 or 2 kg/t of a supplement titrated in PPGs. Blood samples were obtained at 0, 70, 140 and 210 days and assayed for plasma lipid profiles, bilirubin, haematological parameters and indicators of oxidative status (reactive oxygen metabolites (ROMs), thiobarbituric acid reactive substances (TBARS), vitamins A and E). Although dietary treatment did affect the levels of triglycerides, total cholesterol and total bilirubin, all of which decreased markedly (P < 0.05), while significantly increasing the (P < 0.01) HDL cholesterol values, it also significantly improved the oxidative status of the blood, which displayed an increase in both vitamin E (P < 0.01) and vitamin A (P < 0.05) and a decrease in ROMs (P < 0.01) and TBARS (P < 0.05). The improvements in the blood parameters, lipid profile and plasma oxidative status continued to increase significantly as the trial progressed, indicating a positive effect with increased length of treatment. The results of this study demonstrate an important role for feed supplementation with respect to antioxidant activity on some blood parameters, including the lipid profile and the oxidative status of blood.

Keywords

natural extractsverbascosideblood parametershare
Type
Full Paper
Information
animal , Volume 5 , Issue 6 , June 2011 , pp. 844 - 850
Copyright
Copyright © The Animal Consortium 2011

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References

Ali, MN, Hassan, MS, Abd El-Ghany, FA 2007. Effect of strain, type of natural antioxidant and sulphate ion on productive, physiological and hatching performance of native laying hens. International Journal of Poultry Science 6, 539–554.CrossRefGoogle Scholar
Aliyu, R, Gatsing, D, Jaryum, KH 2007. The effects of Boswellia dalzielii (Burseraceae) aqueous bark extract on rat liver function. Asian Journal Biochemical 2, 359–363.Google Scholar
AOAC, 2000. Official Method of Analysis. 17th edition, Association of Official Analytical Chemists, Washington, DC, USA.Google Scholar
Baldassarre, F 2005. Alimentazione della lepre: fabbisogni nutritive e aspetti nutrizionali. Seminario “Aspetti produttivi, gestionali e sanitari nella lepre”. Paganico (GR), 27 maggio 2005, 10–15pp.Google Scholar
Bursill, C, Roach, P 2007. A green tea catechin extract upregulates the hepatic low-density lipoprotein receptor in rats. Lipids 42, 621–627.CrossRefGoogle Scholar
Casamassima, D, Palazzo, M, Presutti, T, Colella, GE 2009. Productive performances, plasmatic oxidative status and some blood parameters in suckling lambs supplemented with verbascoside. In Proceedings of the A.S.P.A. XVIIIth Congress, Palermo, June 9–12, 668pp.Google Scholar
Case, GL, He, L, Mo, H, Elson, CE 1995. Induction of geranyl pyrophosphatase activity by cholesterol suppressive isoprenoids. Lipids 30, 357–359.CrossRefGoogle ScholarPubMed
Castiglione, G, Castellini, C, Dal Bosco, A 1996. Prestazioni riproduttive di lepri allevate in stretta cattività. Rivista di Coniglicoltura 11, 37–40.Google Scholar
Cesarone, MR, Belcaro, G, Caratelli, M 1999. A simple test to monitor oxidative stress. International Angiology 18, 127–130.Google ScholarPubMed
Chen, R, Su, J, Yang, S, Li, J, Wang, T, Zhou, H 2002. Effect of isoverbascoside, a phenylpropanoid glycoside antioxidant, on proliferation and differentiation of human gastric cancer cell. Acta Pharmacological Sinica 23, 997–1001.Google ScholarPubMed
Corino, C, Oriani, G, Pantaleo, L, Pastorelli, G, Salvatori, G 1999. Vitamin E in heavy pigs: Influence of dietary vitamin E supplementation on heavy pig carcass characteristics, meat quality, and vitamin E status. Journal of Animal Science 77, 1755–1761.CrossRefGoogle ScholarPubMed
Corino, C, Rossi, R, Musella, M, Cannata, S, Pastorelli, G 2007a. Growth performance and oxidative status in piglets supplemented with verbascoside and teupolioside. Italian Journal of Animal Science 6 (suppl. 1), 292–294.CrossRefGoogle Scholar
Corino, C, Cannata, S, Musella, M, Pastorelli, G, Rossi, R 2007b. Influence of verbascoside on lipid profile in weaned piglets. Annali of Nutrition and Metabolism 51 (suppl. 1), 270.Google Scholar
Daels-Rakotoarison, DA, Seidel, V, Gressier, B, Brunet, C, Tillequin, F, Bailleul, F, Luyckx, M, Dine, T, Cazin, M, Cazin, JC 2000. Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra. Arzneimittelforschung 50, 16–23.Google ScholarPubMed
Deepak, M, Handa, SS 2000. Antiinflammatory activity and chemical composition of extracts of Verbena officinalis. Phytotherapy Research 14, 463–465.3.0.CO;2-G>CrossRefGoogle ScholarPubMed
Esterbauer, H, Zollner, H 1989. Methods for determinationof aldehydic lipid peroxidation products. Free radical Biology and Medicine 7, 197–203.CrossRefGoogle Scholar
Fidan, AF, Dundar, Y 2008. The effects of Yucca schidigera e Quillaja saponaria on DNA damage, protein oxidation, lipid peroxidation, and some biochemical parameters in streptozotocin-induced diabetic rats. Journal of Diabetes and Its Complications 22, 348–356.CrossRefGoogle Scholar
Gao, JJ, Igalashi, K, Nukina, M 1999. Radical scavenging activity of phenylpropanoid glycosides in Caryopteris incana. Bioscience Biotechnology and Biochemestry 63, 983–988.CrossRefGoogle ScholarPubMed
Halliwell, B 1997. Antioxidants and human diseases: a general introduction. Nutrition Review 55, S44–S52.CrossRefGoogle Scholar
Hidiroglou, M, Farnworth, E, Butler, G 1993. Effects of vitamin E and fat supplementation on concentration of vitamin E in plasma and milk of sows and plasma of piglets. International Journal for Vitamin and Nutrition Research 63, 180–187.Google ScholarPubMed
Kamiloglu, NN, Beytut, E, Aksakal, M 2006. Alteration in antioxidant status and lipid peroxidation of sheep previously treated with vitamin A and b-carotene during breeding and periparturient period. Bulletin of the Veterinary Institute in Pulawy 50, 171–177.Google Scholar
Kunvari, M, Paska, C, Laszlo, M, Orfi, L, Kovesdi, I, Eros, D, Bokonyi, G, Keri, G, Gyurjan, I 1999. Biological activity and structure of antitumor compounds from Plantago media L. Acta Pharmaceutica Hungarica 69, 232–239.Google ScholarPubMed
Lauridsen, C, Engel, H, Jensen, SK, Craig, AM, Traber, MG 2002. Lactating sows and suckling piglets preferentially incorporate RRR-over all-rac-α-tocopherol into milk, plasma and tissues. Journal of Nutrition 132, 1258–1264.CrossRefGoogle ScholarPubMed
Li, JX, Xin, D, Li, H, Lu, JF, Tong, CW, Gao, JN, Chan, KM 1999. Effect of verbascoside on decreasing concentration of oxygen free radicals and lipid peroxidation in skeletal muscle. Chung-Kuo Yao Li Hsueh Pao – Acta Pharmacologica Sinica 20, 126–130.Google ScholarPubMed
Liao, K, Yin, M 2000. Individual and combined antioxidant effects of seven phenolic agents in human erythrocyte membrane ghosts and phosphatidylcholine liposome systems: importance of the partition coefficient. Journal of Agriculture Food Chemical 48, 2266–2270.CrossRefGoogle ScholarPubMed
Liu, M, Li, J, Guo, H, Lee, K, Qin, L, Chan, K 2003. The effects of verbascoside on plasma lipid peroxidation level and erythrocyte membrane fluidity during immobilization in rabbits: a time course study. Life Sciences 73, 883–892.CrossRefGoogle Scholar
Mahan, DC 1991. Assessment of the influence of dietary vitamin E on sows and offspring in three parities: reproductive performances, tissue tocopherol and effects on progeny. Journal of Animal Science 69, 2904–2917.CrossRefGoogle ScholarPubMed
Marco, I, Cuenca, R, Pastor, J, Velarde, R, Lavin, S 2003. Haematology and serum chemistry values of the European Brown hare. Veterinary Clinical Pathology 32, 195–198.CrossRefGoogle ScholarPubMed
Massanyi, P, Slamecka, J, Lukac, N, Capcarova, M, Mertin, D, Jurcik, R 2009. Seasonal variations in the blood biochemistry of brown hare. Medycyna Weterynaryjna 65, 389–393.Google Scholar
Miller, JK, Brzezinska-Slebodzinska, E, Madsen, FC 1993. Oxidative stress, antioxidants and animal function. Journal of Dairy Science 76, 2812–2823.CrossRefGoogle ScholarPubMed
Norata, GD, Pellegatta, F, Catapano, AL 2003. “Peroxisome proliferators actived receptors” e patologie cardivascolari. Italian Heart Journal 4 (suppl.), 8–18.Google Scholar
Nijveldt, RJ, van Nood, E, van Hoorn, DE, Boelens, PG, van Norren, K, van Leewen, PA 2001. Flavonoids: a review of probable mechanisms of action and potential applications. American Journal of Clinical Nutrition 74, 418–425.Google Scholar
Njeru, CA, McDowell, LR, Wilkinson, NS, Linda, SB, Williams, SN 1994. Pre and postpartum supplemental dl-α-tocopheryl acetate effects on placental and mammary vitamin E transfer in sheep. Journal of Animal Science 72, 1636–1640.CrossRefGoogle ScholarPubMed
Oriani, G, Salvatori, G, Pastorelli, G, Pantaleo, L, Ritieni, A, Corino, C 2001. Oxidative status of plasma and muscle in rabbits supplemented with dietary vitamin E. Journal of Nutritional Biochemistry 12, 138–143.CrossRefGoogle ScholarPubMed
Paci, G, Bennati, L, Bagliacca, M 1999. Effect of environmental temperature on daily feed intake and digestibility in hares. Atti LIII Congresso SISVet 16–18 Settembre 1999, 443–444pp.Google Scholar
Paci, G, Bagliacca, M, Mozzicafreddo, S, Lisi, E 2000. Artificial milking of young hares (Lepus europaeus Pallas) born in captivity or in pens. Annali della Facoltà di Medicina Veterinaria di Pisa LII, 209–219.Google Scholar
Paci, G, Lavazza, A, Profumo, A, Ferretti, M, Bagliacca, M 2001. Relationship between habitat traits, census and haematic parameters of Brown Hares. Abstract. Symposium “Decline of European Hares an interdisciplinary European research task”. Berlin, April 18–22, 2001, Abstract, 14.Google Scholar
Piccinelli, A, De Simone, F, Passi, S, Rastrelli, L 2004. Phenolic constituents and antioxidant activity of wendita calysina leaves (Burrito), a folk paraguayan tea. Journal of Agriculture and Food Chemistry 52, 5863–5868.Google Scholar
Princen, HMG, van Duyvenvoorde, W, Buytenhek, R, Blonk, C, Tijburg, LBM, Langius, JAE, Meinders, AE, Pijl, H 1998. No effect of consumption of green and black tea on plasma lipid and antioxidant levels and on LDL oxidation in smokers. Arterioscleris Thrombosis and Vascular Biology 18, 833–841.CrossRefGoogle ScholarPubMed
Radwan, NL 2003. Effect of using some medicinal plants on performance and immunity of broiler chicks. PhD thesis, Poultry Nutrition Department Faculty of Agriculture Cairo University.Google Scholar
Radwan, NL, Hassan, RA, Qota, EM, Fayek, HM 2008. Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. International Journal of Poultry Science 7, 134–150.Google Scholar
SPSS 2009. 16.0 Package program, User's Guide. SPSS Inc., Chicago, Illinois, USA.Google Scholar
Susca, F, Sangalli, L, Sgoifo Rossi, CA, Biondi, PA, Dell'Orto, V 2005. Determinazione della Malonaldeide nel plasma di bovini in fase di adattamento ed effetti della somministrazione con la dieta di fitoderivati. LIX Convegno Nazionale SiSVet, 21–24 Settembre 2005, Viareggio – Italia, 471–472pp.Google Scholar
Wang, P, Kang, J, Zheng, R, Yang, Z, Lu, J, Gao, J, Jia, Z 1996. Scavenging effects of phenylpropanoid glycosides from Pedicularis on superoxide anion and hydroxyl radical by the spin trapping method. Biochemical Pharmacology 51, 687–691.CrossRefGoogle ScholarPubMed
Yousef, MI, Salem, MH, Kamel, KI, Hassan, GA, El-Nouty, FD 2003. Influence of ascorbic acid supplementation on the haematological and clinical biochemistry parameters of male rabbits exposed to Aflatoxin B1. Journal of Environmental Science and Health, Part B 38, 193–209.Google Scholar
Zhao, B, Tham, S-Y, Lu, J, Lai, MH, Lee, LKH, Moochhala, SM 2004. Simultaneous determination of vitamins C, E and β-carotene in human plasma by hogh-performance liquid chromatography with photodiode-array detection. Journal of Pharmacy and Pharmaceutical Sciences 7, 200–204.Google Scholar
Zhu, QY, huang, Y, Tsang, D, Chen, ZY 1999. Regeneration of a-tocopherol in human low-density lipoprotein by green tea catechin. Journal of Agricultural and Food Chemistry 47, 2020–2025.CrossRefGoogle Scholar