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Antioxidative activity and protective effect of probiotics against high-fat diet-induced sperm damage in rats

Published online by Cambridge University Press:  24 July 2012

X. L. Chen ,
L. Z. Gong  and
J. X. Xu
X. L. Chen
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
L. Z. Gong
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
J. X. Xu*
Affiliation:
Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
*
†E-mail: [email protected]
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Abstract

In this study, antioxidant capability and protective effect of probiotics on reproductive damage induced by diet oxidative stress were investigated. Thirty male Sprague-Dawley rats were randomly divided into three groups with 10 rats in each group. The control group consumed a normal standard diet (5% fat, w/w). The other two treatment groups were fed with a high-fat diet (20% fat, w/w), and a high-fat diet supplemented with 2% probiotics (w/w), respectively. At the end of the experimental period, that is, after 6 weeks, rats were killed. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), contents of nitric oxide (NO) free radical and malondialdehyde (MDA) in serum and sperm suspension were examined. Sperm parameters including sperm concentration, viability, motility and DNA integrity were analyzed. The results showed that high-fat diet could induce oxidative stress, shown as significant increases in lipid peroxidation, NO free radical, significant decrease in activities of SOD, GSH-Px, significant reduction in sperm concentration, viability and motility, and damage in sperm DNA (P < 0.05), compared with the control group. These alterations were significantly reversed in the probiotics-supplemented group and had no significant difference in antioxidant capability, lipid peroxidation and sperm parameters compared with the control group. The percentage of sperm with DNA damage was significantly lower than the high-fat diet group and still higher than the control group, which means that probiotics could attenuate sperm damage to some extent. The present results indicated that dietary probiotics had antioxidant activity and the protective effect against sperm damage induced by high-fat diet to some extent.

Keywords

probioticsantioxidantoxidative stresssperm
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 2 , February 2013 , pp. 287 - 292
Copyright
Copyright © The Animal Consortium 2012

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References

Chen, XL, Sun, TT, Xu, JX 2010. Antioxidation and damage repair ability of microbe-derived antioxidants in rats. China Feed (China) 426, 11–15.Google Scholar
Cui, J, Le, GW, Yang, RL, Shi, YH 2009. Lipoic acid attenuates high fat diet-induced chronic oxidative stress and immunosuppression in mice jejunum: a microarray analysis. Cellular Immunology 260, 44–50.Google Scholar
D'Silva, J, Stevenson, P 2011. Farm animal breeding – the implications of existing and new technologies. In Sustainable agricultural development: recent approaches in resources management and environmentally-balanced production enhancement (ed. M Behnassi, SA Shahid and J D'Silva), pp. 215–223. Springer, Dordrecht, The Netherlands.Google Scholar
Dantzer, R, Mormede, P 1983. Stress in farm animals: a need for reevaluation. Journal of Animal Science 57, 6–18.Google Scholar
Feillet-Coudray, C 2009. Oxidative stress in rats fed a high-fat high-sucrose diet and preventive effect of polyphenols: involvement of mitochondrial and NAD(P)H oxidase systems. Free Radical Biology and Medicine 46, 624–632.Google Scholar
Flaherty, CO, De Lamirande, E, Gagnon, C 2006. Positive role of reactive oxygen species in mammalian sperm capacitation: triggering and modulation of phosphorylation events. Free Radical Biology and Medicine 41, 528–540.Google Scholar
Gaggìa, F, Mattarelli, P, Biavati, B 2010. Probiotics and prebiotics in animal feeding for safe food production. International Journal of Food Microbiology 141, S15–S28.CrossRefGoogle ScholarPubMed
Gagnon, C, Iwasaki, A, De Lamirande, E, Kowalski, N 1991. Reactive oxygen species and human spermatozoa. Annals of the New York Academy of Sciences 637, 436–444.Google Scholar
Greco, E, Iacobelli, M, Rienzi, L, Ubaldi, F, Ferrero, S, Tesarik, J 2005. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. Journal of Andrology 26, 349–353.Google Scholar
Kistanova, E 2005. Improvement of the reproductive performances of rams by the biological active substances – plant extract and probiotic. Biotechnology in Animal Husbandry 21, 69–72.CrossRefGoogle Scholar
Le Bon, M, Davies, HE, Glynn, C, Thompson, C, Madden, M, Wiseman, J, Dodd, CER, Hurdidge, L, Payne, G, Le Treut, Y, Craigon, J, Totemeyer, S, Mellits, KH 2010. Influence of probiotics on gut health in the weaned pig. Livestock Science 133, 179–181.CrossRefGoogle Scholar
Lowry, OH, Rosebrough, NJ, Farr, AL, Randall, RJ 1951. Protein measurement with Folin phenol reagent. The Journal of Biological Chemistry 193, 265–275.Google Scholar
Menezo, YJ, Hazout, A, Panteix, G, Robert, F, Rollet, J, Cohen-Barcrie, P, Chapuis, F, Clement, P, Benkhalifa, M 2007. Antioxidants to reduce sperm DNA fragmentation: an unexpected adverse effect. Reproductive Biomedicine Online 14, 418–421.Google Scholar
Mikelsaar, M, pirje hütt, P, Kullisaar, T, Zilmer, K, Zilmer, M 2008. Double benefit claims for antimicrobial and antioxidative probiotic. Microbial Ecology in Health and Disease 20, 184–188.Google Scholar
Miller, JK, Brzezinska-Slebodzinska, E 1993. Oxidative stress, antioxidants, and animal function. Journal of Dairy Science 76, 2812–2823.Google Scholar
Sies, H 1991. Oxidative stress: oxidants and antioxidants. Academic Press, San Diego, CA, USA.Google Scholar
Slupphaug, G, Kavli, B, Krokan, HE 2003. The interacting pathways for prevention and repair of oxidative DNA damage. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 531, 231–251.Google Scholar
Sreekumar, R, Unnikrishnan, J, Fu, A, Nygren, J, Short, KR 2002. Impact of high-fat diet and antioxidant supplement on mitochondrial functions and gene transcripts in rat muscle. American Journal of Physiology – Endocrinology and Metabolism 282, E1055–E1061.Google Scholar
Su, HL, Li, RS, He, XR, Chen, JZ, Han, ZZ, Liu, Y, Zhang, JF 2009. Effects of selenium-enriched probiotics on sperm quality of stock boars. Guangdong Agricultural Sciences 7, 156–158.Google Scholar
Suresh, S, Prithiviraj, E, Prakash, S 2010. Effect of Mucuna pruriens on oxidative stress mediated damage in aged rat sperm. International Journal of Andrology 33, 22–32.Google Scholar
Syntin, P, Robaire, B 2001. Sperm structural and motility changes during aging in the brown Norway rat. Journal of Andrology 22, 235–244.Google Scholar
Tarin, JJ, Brines, J, Cano, A 1998. Antioxidants may protect against infertility. Human Reproduction 13, 1415–1424.Google Scholar
Tovar-Ramírez, V, Mazurais, D, Gatesoupe, JF, Quazuguel, P, Cahu, CL, Zambonino-Infante, JL 2010. Dietary probiotic live yeast modulates antioxidant enzyme activities and gene expression of sea bass (Dicentrarchus labrax) larvae. Aquaculture 300, 142–147.Google Scholar
World Health Organization 2010. WHO Laboratory manual for the examination and processing of human semen, 5th edition. World Health Organization, Geneva, Switzerland.Google Scholar