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9cis,11trans conjugated linoleic acid (CLA) is synthesised and desaturated into conjugated 18:3 in bovine adipose tissues

Published online by Cambridge University Press:  01 April 2008

D. Gruffat ,
C. Rémond ,
D. Durand ,
O. Loreau  and
D. Bauchart
D. Gruffat*
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
C. Rémond
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
D. Durand
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
O. Loreau
Affiliation:
Commissariat à l’Energie Atomique (CEA) Saclay, F-91191 Gif/Yvette Cedex, France
D. Bauchart
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
*
E-mail: [email protected]
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Abstract

Although endogenous synthesis of conjugated linoleic acid (CLA) in the mammary gland of lactating cows has been already well documented, no study has determined so far as to which tissue and/or organ is involved in CLA synthesis in the growing ruminant except one study showing that CLA synthesis does not occur in ruminant liver. In this context, adipose tissue appears to be a good candidate for endogenous synthesis of CLA in the growing ruminant. The aim of this study was to compare the respective metabolisms of 11trans 18:1 (vaccenic acid, VA) and 9cis,11trans 18:2 (rumenic acid) to that of stearic acid (the preferential substrate of Δ9 desaturase) in adipose tissues (subcutaneous, SC and intermuscular, IM) of six Charolais steers by using the in vitromethod of incubated tissue slices. Samples of SC and IM adipose tissues were incubated at 37°C for 16 h under an atmosphere of 95% O2/5% CO2 in a medium supplemented with 0.75 mM of fatty acid (FA) mixture (representative of circulating non-esterified FA) and 186 μM [1-14C]-18:0 or 58.6 μM [1-14C]-VA or 56 μM [1-14C]-9cis,11trans CLA. Viability of explants was verified by measuring metabolic functions (glucose uptake and glucose-6-phosphate dehydrogenase activity). After 16 h of incubation, FA uptake was similar for all FA (18:0, VA and 9cis,11trans 18:2) in both SC and IM adipose tissues (around 40%). Once in adipose tissue, all FA were preferentially esterified (>80% of cell FA) favouring neutral lipid synthesis (around 90% of esterified FA). Stearic acid was highly (27%) desaturated into oleic acid in SC adipose tissue whereas this desaturation was much lower (6.8%) in IM adipose tissue (P < 0.0001). VA was desaturated into 9cis,11trans CLA at a low extent of about 2.5% to 4.4% in both adipose tissues probably because of a limited affinity of Δ9 desaturase for VA. 9cis,11trans CLA was itself converted by desaturation into 6cis, 9cis,11trans 18:3 at the intensity of 10.8% and 14.5% of cell 9cis,11trans CLA in SC and IM adipose tissues, respectively. In conclusion, bovine adipose tissues of the growing ruminant were especially involved in the endogenous synthesis of CLA from VA and in its desaturation into conjugated derivative, mainly 6cis, 9cis,11trans 18:3, of which biological properties need to be elucidated.

Keywords

vaccenic acidconjugated linoleic acidadipose tissuemetabolismbovine
Type
Full Paper
Information
animal , Volume 2 , Issue 4 , April 2008 , pp. 645 - 652
Copyright
Copyright © The Animal Consortium 2008

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References

Archibeque, SL, Lunt, DK, Gilbert, CD, Tume, RK, Smith, SB 2005. Fatty acid indices of stearoyl-CoA desaturase do not reflect actual stearoyl-CoA desaturase enzyme activities in adipose tissues of beef steers finished with corn-, flaxseed-, or sorghum-based diets. Journal of Animal Science 83, 1153–1166.CrossRefGoogle ScholarPubMed
Banni, S, Day, BW, Evans, RW, Corongiu, FP, Lombardi, B 1995. Detection of conjugated diene isomers of linoleic acid in liver lipids of rats fed choline-devoid diet indicates that the diet does not cause lipoperoxidation. Journal of Nutritional Biochemistry 6, 281–289.CrossRefGoogle Scholar
Banni, S, Carta, G, Contini, MS, Angioni, E, Deiana, M, Dessi, MA, Melis, MP, Corongiu, FP 1996. Characterization of conjugated diene fatty acids in milk, dairy products, and lamb tissues. Journal of Nutritional Biochemistry 7, 150–155.Google Scholar
Bauchart, D, Gladine, C, Gruffat, D, Leloutre, L, Durand, D 2005. Effects of diets supplemented with oil seeds and vitamin E on specific fatty acids of Rectus abdominis muscle in Charolais fattening bulls. In Indicators of milk and beef quality (ed. JF Hocquette and S Gigli), EAAP publication no. 112, pp. 431–436. Wageningen Academic Publishers, Wageningen, The Netherlands.CrossRefGoogle Scholar
Bauman, DE, Baumgard, LH, Corl, BA, Griinari, JM 1999. Biosynthesis of conjugated linoleic acid in ruminants. Proceeding of the American Society of Animal Science 1–15.Google Scholar
Belury, MA, KempaSteczko, A 1997. Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32, 199–204.CrossRefGoogle ScholarPubMed
Bretillon, L, Chardigny, JM, Grégoire, S, Berdeaux, O, Sébédio, JL 1999. Effects of conjugated linoleic acid isomers on the hepatic microsomal desaturation activities in vitro. Lipids 34, 965–969.CrossRefGoogle ScholarPubMed
Cameron, PJ, Rogers, M, Oman, J, May, SG, Lunt, DK, Smith, SB 1994. Stearoyl CoA desaturase enzyme activity and mRNA levels are not different in subcutaneous adipose tissue from Angus and American Wagyu steers. Journal of Animal Science 72, 2624–2628.Google Scholar
Chang, JH, Lunt, DK, Smith, SB 1992. Fatty acid composition and fatty acid elongase and stearoyl-CoA desaturase activities in tissues of steers fed high oleate sunflower seed. Journal of Nutrition 122, 2074–2080.CrossRefGoogle ScholarPubMed
Chilliard, Y 1993. Dietary fat and adipose tissue metabolism in ruminants, pigs and rodents: a review. Journal of Dairy Science 76, 3897–3931.Google Scholar
Chilliard, Y, Faulconnier, Y 1995. Insulin and (or) dexamethasone regulation of glucose and acetate utilization in ovine and bovine adipose tissue explants incubated for seven days. Journal of Animal Science 73, 2063–2070.CrossRefGoogle ScholarPubMed
Chilliard, Y, Gagliostro, G, Flechet, J, Lefaivre, J, Sebastian, I 1991. Duodenal rapeseed oil infusion in early and midlactation cows. 5. Milk fatty acids and adipose tissue lipogenic activities. Journal of Dairy Science 74, 1844–1854.CrossRefGoogle ScholarPubMed
Christie, WW, Sébédio, JL, Juanéda, P 2001. A practical guide to the analysis of conjugated linoleic acid. Inform 12, 147–152.Google Scholar
Corl, BA, Baumgard, LH, Dwyer, DA, Griinari, JM, Phillips, BS, Bauman, DE 2001. The role of Δ9-desaturase in the production of cis-9, trans-11 CLA. Journal of Nutrition and Biochemistry 12, 622–630.CrossRefGoogle Scholar
De La Torre, A, Gruffat, D, Chardigny, JM, Sébédio, JL, Durand, D, Loreau, O, Bauchart, D 2005a. In vitro metabolism of rumenic acid in bovine liver slices. Reproduction Nutrition and Development 45, 441–451.Google Scholar
De La Torre, A, Debiton, E, Durand, D, Chardigny, JM, Berdeaux, O, Loreau, O, Barthomeuf, C, Bauchart, D, Gruffat, D 2005b. Conjugated linoleic acid isomers and their conjugated derivatives inhibit growth of human cancer cell lines. Anticancer Research 25, 3943–3950.Google ScholarPubMed
Faulconnier, Y, Thevenet, M, Flechet, J, Chilliard, Y 1994. Lipoproteine lipase and metabolic activities in incubated bovine adipose tissue explants: effects of insulin, dexamethasone, and fetal bovine serum. Journal of Animal Science 72, 184–191.CrossRefGoogle ScholarPubMed
Folch, J, Lees, MSloane-Stanley, GH 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.Google Scholar
Gilham, D, Lehner, R 2006. Provision of lipids for very low-density lipoprotein assembly. In Biochemistry of atherosclerosis (ed. SK Cheema), pp. 121–149. Springer, NY.Google Scholar
Graulet, B, Gruffat, D, Durand, D, Bauchart, D 1998. Fatty acid metabolism and very low density lipoprotein secretion in liver slices from rats and preruminant calves. Journal of Biochemistry 124, 1212–1219.Google Scholar
Griinari, JM, Corl, BA, Chouinard, PY, Nurmela, KV, Bauman, DE 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)-desaturase. Journal of Nutrition 130, 2285–2291.Google Scholar
Gruffat, D, De La Torre, A, Chardigny, JM, Durand, D, Loreau, O, Sébédio, JL, Bauchart, D 2003. In vitro comparison of hepatic metabolism of 9cis-11trans and 10trans-12cis isomers of CLA in the rat. Lipids 38, 157–163.CrossRefGoogle ScholarPubMed
Gruffat, D, De La Torre, A, Chardigny, JM, Durand, D, Loreau, O, Bauchart, D 2005. Vaccenic acid metabolism in the liver of rat and bovine. Lipids 40, 295–301.Google Scholar
Hanson, RW, Ballard, FJ 1967. The relative significance of acetate and glucose as precursors for lipid synthesis in liver and adipose tissue from ruminants. Biochemical Journal 105, 529–536.Google Scholar
Jacobi, SK, Miner, JL 2002. Human acylation stimulating protein and lipid biosynthesis in bovine adipose tissue explants. Journal of Animal Science 80, 751–756.Google Scholar
Jeffcoat, R, Brawn, PR, Safford, R, James, AT 1977. Properties of rat liver microsomal stearoyl-coenzyme A desaturase. Biochemical Journal 161, 431–437.Google Scholar
Kaluzny, MA, Rode, LM, Meritt, MV, Epps, DE 1985. Rapid separation of lipid classes in high yield and purity using bonded phase columns. Journal of Lipid Research 26, 135–140.CrossRefGoogle ScholarPubMed
Novakofski, J 2004. Adipogenesis: usefulness of in vitro and in vivo experimental models. Journal of Animal Science 82, 905–915.Google Scholar
Pariza, MW, Park, Y, Cook, ME 2001. The biologically active isomers of conjugated linoleic acid. Progress in Lipid Research 40, 283–298.Google Scholar
Raes, K, De Smet, S, Balcaen, A, Clayes, E, Demeyer, D 2003. Effect of diets rich in N-3 polyunsaturated fatty acids on muscle lipids and fatty acids in Belgian Blue double-muscled young bulls. Reproduction, Nutrition Development 43, 331–345.CrossRefGoogle Scholar
Ritzenthaler, KL, Mc Guire, MK, Falen, R, Shultz, TD, Dasgupta, N, Mc Guire, MA 2001. Estimation of CLA intake by written dietary assessment methodologies underestimates actual intake evaluated by food duplicate methodology. Journal of Nutrition 131, 1548–1554.CrossRefGoogle ScholarPubMed
Santora, JE, Palmquist, DL, Roehrig, KL 2000. Trans-vaccenic acid is desaturated to conjugated linoleic acid in mice. Journal of Nutrition 130, 208–215.CrossRefGoogle ScholarPubMed
Sébédio, JL, Juanéda, P, Dobson, G, Ramilison, I, Martin, JC, Chardigny, JM, Christie, WW 1997. Metabolites of conjugated isomers of linoleic acid (CLA) in the rat. Biochemical and Biophysical Acta 1345, 5–10.CrossRefGoogle ScholarPubMed
Sébédio, JL, Angioni, E, Chardigny, JM, Grégoire, S, Juanéda, P, Berdeaux, O 2001. The effect of conjugated linoleic acid isomers on fatty acid profiles of liver and adipose tissues and their conversion to isomers of 16:2 and 18:3 conjugated fatty acids in rats. Lipids 36, 575–582.Google Scholar
Siebert, BD, Pitchford, WS, Kruk, ZA, Kuchel, H, Deland, MP, Bottema, CD 2003. Differences in Δ9 desaturase activity between Jersey and Limousin-Sired cattle. Lipids 38, 539–543.CrossRefGoogle ScholarPubMed
St John, LC, Lunt, DK, Smith, SB 1991. Fatty acid elongation and desaturation enzyme activities of bovine liver and subcutaneous adipose tissue microsomes. Journal of Animal Science 69, 1064–1073.CrossRefGoogle ScholarPubMed
Vernon, RG, Barber, MC, Finley, DJ 1991. Modulation of the activity of acetyl-CoA carboxylase and other lipogenic enzymes by growth hormone, insulin and dexamethasone in sheep adipose tissue and relationship to adaptations to lactation. Biochemical Journal 274, 543–548.Google Scholar
Ward, RJ, Travers, MT, Richards, SE, Vernon, RG, Salter, AM, Buttery, PJ, Barber, MC 1998. Stearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome. Biochemical and Biophysical Acta 1391, 145–156.CrossRefGoogle ScholarPubMed
Whale, K, Heys, SD, Rotondo, D 2004. Conjugated linoleic acids: are they beneficial or detrimental to health? Progress in Lipid Research 43, 553–587.Google Scholar