Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-22T17:31:34.331Z Has data issue: false hasContentIssue false

On-farm culling methods used for pigs

Published online by Cambridge University Press:  01 January 2023

FA Dalla Costa*
Affiliation:
MSD Animal Health, Department of Strategy and Innovation, Av Dr Chicri Zaidan, 296, São Paulo - SP Brazil Programa de Pós-graduação em Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14.884-900 Jaboticabal, SP, Brazil Maneja bem-estar animal, MANEJA, 89.710-037 Concódia, SC, Brazil
TJ Gibson
Affiliation:
Royal Veterinary College, University of London, Hawkshead Lane, Hatfield AL9 7TA, UK
SEO Oliveira
Affiliation:
Programa de Pós-graduação em Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14.884-900 Jaboticabal, SP, Brazil Maneja bem-estar animal, MANEJA, 89.710-037 Concódia, SC, Brazil
NG Gregory
Affiliation:
Royal Veterinary College, University of London, Hawkshead Lane, Hatfield AL9 7TA, UK
L Faucitano
Affiliation:
Agriculture and Agri-Food Canada, 2000 College Street, J1M 0C8 Sherbrooke, Quebec, Canada
OA Dalla Costa
Affiliation:
Embrapa Swine and Poultry, BR 153, Km 110, Concórdia 89700-991, Brazil
*
* Contact for correspondence: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The culling of injured and non-viable pigs (Sus scrofa) (neonate to breeding stock) is a routine and necessary procedure on most farms. Usually, pigs are culled using one of the following methods: blunt-force trauma (manual and mechanical), captive-bolt stunners, electrical stunning and electrocution or carbon dioxide. Manual blunt-force trauma is one of the most widely used methods due to its low or absent operational and investment costs. However, as a method, it has serious limitations, which include the risk of incomplete concussion, pain, and distress. Manual blunt-force trauma is also aesthetically unpleasant to operators and wider society. To address these issues there has been significant recent research into the development of alternatives to manual blunt-force trauma, these include: captive-bolt stunners, on-farm, gas-based controlled atmosphere systems, low atmospheric pressure systems and electrical stunning. Some of these are currently in commercial use while others are still in the developmental phase. This review brings together the relevant research in this field, evaluating the methods in terms of mechanism of action (mechanical and physiological), effectiveness and animal welfare.

Type
Research Article
Copyright
© 2021 Universities Federation for Animal Welfare

References

American Veterinary Medical Association (AVMA) 2008 Literature review on the welfare implications of electroimmobi-lization. Animal Welfare Division: 1-3. https://admin.avma.org/KB/Resources/LiteratureReviews/Docume nts/electroimmobilization_bgnd.pdfGoogle Scholar
American Veterinary Medical Association (AVMA) 2020 AVMA Guidelines for the euthanasia of animals: 2020 edition. Journal of the American Veterinary Medical Association 2013: 1121Google Scholar
Anil, MH 2012 Effects of slaughter method on carcass and meat characteristics in the meat of cattle and sheep. EBLEX— A division of the Agriculture and Horticulture Development Board p 73. EBLEX: UKGoogle Scholar
Anil, MH and McKinstry, JL 1992 The effectiveness of high fre-quency electrical stunning in pigs. Meat Science 31: 481491. https://doi.org/10.1016/0309-1740(92)90030-8CrossRefGoogle Scholar
Anon 2010 Defra final report MH0128: Novel and humane gaseous killing methods for pigs. Defra: London, UKGoogle Scholar
Anon 2016 Defra final report MH0150: Study to investigate non-pen-etrating percussive blow to the head as a humane killing method for piglets, kids and lambs up to 5 kg. Defra: London, UKGoogle Scholar
Anton, F, Euchner, I and Handwerker, HO 1992 Psychophysical examination of pain induced by defined CO2 pulses applied to the nasal mucosa. Pain 49: 5360. https://doi.org/10.1016/0304-3959(92)90187-GCrossRefGoogle Scholar
Baker, HJ and Scrimgeour, HJ 1995 Evaluation of methods for the euthanasia of cattle in a foreign animal disease outbreak. The Canadian Veterinary Journal 36: 160165Google Scholar
Banzett, RB, Gracely, RH and Lansing, RW 2007 When it's hard to breathe, maybe pain doesn't matter. Focus on ‘Dyspnea as a noxious sensation: inspiratory threshold loading may trigger dif-fuse noxious inhibitory controls in humans’. Journal of Neurophysiology 97: 959960. https://doi.org/10.1152/jn.00970.2006CrossRefGoogle ScholarPubMed
Banzett, RB, Lansing, RW, Evans, KC and Shea, SA 1996 Stimulus-response characteristics of CO2-induced air hunger in normal subjects. Respiration Physiology 103: 1931. https://doi.org/10.1016/0034-5687(95)00050-XCrossRefGoogle ScholarPubMed
Beausoleil, NJ and Mellor, DJ 2015 Introducing breathlessness as a significant animal welfare issue. New Zealand Veterinary Journal 63: 4451. https://doi.org/10.1080/00480169.2014.940410CrossRefGoogle ScholarPubMed
Berg, C and Raj, M 2015 A review of different stunning methods for poultry. Animal welfare aspects (stunning methods for poul-try). Animals 5: 12071219. https://doi.org/10.3390/ani5040407CrossRefGoogle Scholar
Betz, P, Pankratz, H, Penning, R and Eisenmenger, W 1993 Homicide with a captive bolt pistol. The American Journal of Forensic Medicine and Pathology 14: 5457. https://doi.org/10.1097/00000433-199303000-00013CrossRefGoogle ScholarPubMed
Blackmore, DK 1985 Energy requirements for the penetration of heads of domestic stock and the development of a multiple projectile. The Veterinary Record 116: 3640. https://doi.org/10.1136/vr.116.2.36CrossRefGoogle ScholarPubMed
Blackmore, DK, Bowling, MC, Madie, P, Nutman, A, Barnes, GRG, Davies, AS, Donoghue, M and Kirk, EJ 1995 The use of a shotgun for the emergency slaughter or euthanasia of large mature pigs. New Zealand Veterinary Journal 43: 134137. https://doi.org/10.1080/00480169.1995.35872CrossRefGoogle ScholarPubMed
Blackmore, DK and Newhook, JC 1981 Insensibility during slaughter of pigs in comparison to other domestic stock. New Zealand Veterinary Journal 29: 219222. https://doi.org/10.1080/00480169.1981.34850CrossRefGoogle ScholarPubMed
Blumbergs, PC 1997 Pathology. In: Reilly, P and Bullock, R (eds) Head Injury. Pathophysiology and Management of Severe Closed Injury pp 3970. Chapman and Hall Medical: London, UKGoogle Scholar
Bouwsema, JA and Lines, JA 2019 Could low atmospheric pressure stunning (LAPS) be suitable for pig slaughter? A review of available information. Animal Welfare 28: 421432. https://doi.org/10.7120/09627286.28.4.421CrossRefGoogle Scholar
Burnett, BR 1991 Detection of bone and bone-plus-bullet parti-cles in backspatter from close-range shots to heads. Journal of Forensic Science 36: 17451752. https://doi.org/10.1520/JFS13199JCrossRefGoogle Scholar
Buzzard, BL 2012 Evaluation of hypobaric hypoxia as a low stress alternative to carbon dioxide euthanasia for use with nursery piglets. Doctoral dissertation, Kansas State University, USAGoogle Scholar
Caird, G, Roberts, M, Farrell, D and Allcutt, J 2000 Self-inflict-ed head trauma using a captive bolt pistol: report of three cases. British Journal of Neurosurgery 14: 349351. https://doi.org/10.1080/026886900417360CrossRefGoogle ScholarPubMed
Casey-Trott, TM, Millman, ST, Turner, PV, Nykamp, SG, Lawlis, PC and Widowski, TM 2014 Effectiveness of a non-penetrating captive bolt for euthanasia of 3 kg to 9 kg pigs. Journal of Animal Science 92: 51665174. https://doi.org/10.2527/jas.2014-7980CrossRefGoogle Scholar
Casey-Trott, TM, Millman, ST, Turner, PV, Nykamp, SG and Widowski, TM 2013 Effectiveness of a non-penetrating captive bolt for euthanasia of piglets less than 3 d of age. Journal of Animal Science 91: 54775484. https://doi.org/10.2527/jas.2013-6320CrossRefGoogle Scholar
CFMV 2012 Resolução No 1000 de 11 de Maio de 2012. Conselho Federal de Medicina Veterinária - CFMV: 1-9Google Scholar
Chen, X, Gallar, J, Pozo, MA, Baeza, M and Belmonte, C 1995 CO2 stimulation of the cornea: a comparison between human sen-sation and nerve activity in polymodal nociceptive afferents of the cat. European Journal of Neuroscience 7: 11541163. https://doi.org/10.1111/j.1460-9568.1995.tb01105.xCrossRefGoogle Scholar
Cooney, KA, Chappell, JR, Callan, RJ and Connally, B 2012 Veterinary Euthanasia Techniques, A Practical Guide pp 136139. Wiley Blackwell: Hoboken, NJ, USA. https://doi.org/10.1002/9781118704585CrossRefGoogle Scholar
Council Directive 1099/2009 2009 Council Regulation No 1099/2009 on the protection of animals at the time of killing. Official Journal of the European Union L303: 1-30Google Scholar
Dalla Costa, FA, Gibson, TJ, Oliveira, SEO, Gregory, NG, Coldebella, A, Faucitano, L and Dalla Costa, OA 2019 On-farm pig dispatch methods and stockpeople attitudes on their use. Livestock Science 221: 15. https://doi.org/10.1016/j.livsci.2019.01.007CrossRefGoogle Scholar
Dalla Costa, FA, Gibson, TJ, Oliveira, SEO, Gregory, NG, Coldebella, A, Faucitano, L, Ludtke, CB, Buss, LP and Dalla Costa, OA 2020 Evaluation of physical euthanasia for neonatal piglets on-farm. Animal Science 98: 111. https://doi.org/10.1093/jas/skaa204Google ScholarPubMed
Dalla Costa, FA, Oliveira, SEO, Gregory, NG, Dalla Costa, OA, Paranhos da Costa, MJR, Gibson, TJ and Coldebella, A 2016 The role of cartridge power in performance of captive bolt gun pp 323324. PorkExpo 2016, Foz do Iguacú: Parana, BrazilGoogle Scholar
Dalmau, A, Rodriguez, P, Llonch, P and Velarde, A 2010 Stunning pigs with different gas mixtures: aversion in pigs. Animal Welfare 19: 325333Google Scholar
Danneman, PJ, Stein, S and Walshaw, SO 1997 Humane and practical implications of using carbon dioxide mixed with oxy-gen for anesthesia or euthanasia of rats. Laboratory Animal Science 47: 376385Google ScholarPubMed
Dawson, MD, Benson, ER, Malone, GW, Alphin, RL, Estevez, I and Van Wicklen, GL 2006 Evaluation of foam-based mass depopulation methodology for floor-reared meat-type poultry operations. Applied Engineering in Agriculture 22: 787794. https://doi.org/10.13031/2013.21984CrossRefGoogle Scholar
De la Cruz, L, Gibson, TJ, Guerrero-Legarreta, I, Napolitano, F, Mora-Medina, P and Mota-Rojas, D 2018 The welfare of water buffaloes during the slaughter process: a review. Livestock Science 212: 2223. https://doi.org/10.1016/j.livsci.2018.03.014CrossRefGoogle Scholar
Denicourt, M, Klopfenstein, C, Dufour, C, Pouliot, F and D’Allaire, S 2010 Using an electrical approach to euthanize pigs on-farm: fundamental principles to know. Annual Meeting: Implementing Knowledge pp 451468. AASVA (American Association of Swine Veterinarians): Omaha, Nebraska, USAGoogle Scholar
Dripps, RD and Comroe, JH 1947 The respiratory and circula-tory response of normal man to inhalation of 7.6 and 10.4 per cent CO2 with a comparison of the maximal ventilation produced by severe muscular exercise, inhalation of CO2 and maximal vol-untary hyperventilation. American Journal of Physiology—Legacy Content 149: 4351. https://doi.org/10.1152/ajplegacy.1947.149.1.43CrossRefGoogle Scholar
Edwards, L and Engle, T 2011 Evaluation and application of humane hypoxia euthanasia for nursery pigs. Des Moines, Iowa: Pork Checkoff Research Review Newsletter 235-241Google Scholar
Engle, TE and Edwards, LN 2010 Evaluation and application of humane hypoxia euthanasia for nursery pigs. Pork Checkoff Research Review Newsletter, Des Moines, IA, USAGoogle Scholar
European Food Safety Authority (EFSA) 2018 Commission implementing regulation (EU) 2018/723. EFSA: Brussels, BelgiumGoogle Scholar
European Food Safety Authority (EFSA) 2004 Welfare aspects of animal stunning and killing methods. Scientific Report of the Scientific Panel for Animal Health and Welfare on a request from the Commission related to welfare aspects of animal stun-ning and killing methods. EFSA Journal, 2: 129. https://doi.org/10.2903/j.efsa.2004.45Google Scholar
Farouk, MM 2013 Advances in the industrial production of halal and kosher red meat. Meat Science 95: 805820. https://doi.org/10.1016/j.meatsci.2013.04.028CrossRefGoogle ScholarPubMed
Feng, Y and Simpson, TL 2003 Nociceptive sensation and sensi-tivity evoked from human cornea and conjunctiva stimulated by CO2. Investigative Ophthalmology & Visual Science 44: 529532. https://doi.org/10.1167/iovs.02-0003CrossRefGoogle Scholar
Fiedler, KJ, Parsons, RL, Sadler, LJ and Millman, ST 2016 Effects of stocking rate on measures of efficacy and welfare during argon gas euthanasia of weaned pigs. Animal Welfare 25: 8389. https://doi.org/10.7120/09627286.25.1.083CrossRefGoogle Scholar
Finnie, J, Lewis, S, Manavis, J, Blumbergs, P, Van Den Heuvel, C and Jones, N 1999 Traumatic axonal injury in lambs: a model for paediatric axonal damage. Journal of Clinical Neuroscience 6: 3842. https://doi.org/10.1016/S0967-5868(99)90601-XCrossRefGoogle Scholar
Finnie, JW 1993 Pathology of experimental traumatic cranio-cerebral missile injury. Journal of Comparative Pathology 108: 93101. https://doi.org/10.1016/S0021-9975(08)80231-9CrossRefGoogle Scholar
Finnie, JW 2016 Forensic pathology of traumatic brain injury. Veterinary Pathology 53: 962978. https://doi.org/10.1177/0300985815612155CrossRefGoogle ScholarPubMed
Finnie, JW, Blumbergs, PC, Manavis, J, Summersides, GE and Davies, RA 2000 Evaluation of brain damage resulting from penetrating and non-penetrating captive bolt stunning using lambs. Australian Veterinary Journal 78: 775778. https://doi.org/10.1111/j.1751-0813.2000.tb10451.xCrossRefGoogle ScholarPubMed
Finnie, JW, Manavis, J, Summersides, GE and Blumbergs, PC 2003 Brain damage in pigs produced by impact with a nonpene-trating captive bolt pistol. Australian Veterinary Journal 81: 153155. https://doi.org/10.1111/j.1751-0813.2003.tb11078.xCrossRefGoogle ScholarPubMed
Finnie, JW, Van den Heuvel, C, Gebski, V, Manavis, J, Summersides, GE and Blumbergs, PC 2001 Effect of impact on different regions of the head of lambs. Journal of Comparative Pathology 124: 159164. https://doi.org/10.1053/jcpa.2000.0446CrossRefGoogle ScholarPubMed
Forslid, A 1987 Transient neocortical, hippocampal and amyg-daloid EEG silence induced by one minute inhalation of high con-centration CO2 in swine. Acta Physiologica 130: 110. https://doi.org/10.1111/j.1748-1716.1987.tb08104.xCrossRefGoogle Scholar
Gaetz, M 2004 The neurophysiology of brain injury. Clinical Neurophysiology 115: 418. https://doi.org/10.1016/S1388-2457(03)00258-XCrossRefGoogle ScholarPubMed
Gibson, TJ, Bedford, EM, Chancellor, NM and Limon, G 2015a Pathophysiology of free-bullet slaughter of horses and ponies. Meat Science 108: 120124. https://doi.org/10.1016/j.meatsci.2015.06.007CrossRefGoogle ScholarPubMed
Gibson, TJ, Mason, CW, Spence, JY, Barker, H and Gregory, NG 2015b Factors affecting penetrating captive bolt gun perform-ance. Journal of Applied Animal Welfare Science 18: 222238. https://doi.org/10.1080/10888705.2014.980579CrossRefGoogle Scholar
Gibson, TJ, Oliveira, SEO, Dalla Costa, FAD and Gregory, NG 2019 Electroencephalographic assessment of pneumatically powered penetrating and non-penetrating captive-bolt stunning of bulls. Meat Science 151: 5459. https://doi.org/10.1016/j.meatsci.2019.01.006CrossRefGoogle ScholarPubMed
Gibson, TJ, Rebelo, CB, Gowers, TA and Chancellor, NM 2018 Electroencephalographic assessment of concussive non-pen-etrative captive bolt stunning of turkeys. British Poultry Science 59: 1320. https://doi.org/10.1080/00071668.2017.1401215Google ScholarPubMed
Gibson, TJ, Ridler, AL, Lamb, CR, Williams, A, Giles, S and Gregory, NG 2012 Preliminary evaluation of the effectiveness of captive-bolt guns as a killing method without exsanguination for horned and unhorned sheep. Animal Welfare 21: 3542. https://doi.org/10.7120/096272812X13353700593446CrossRefGoogle Scholar
Gibson, TJ, Whitehead, C, Taylor, R, Sykes, O, Chancellor, NM and Limon, G 2015c Pathophysiology of penetrating captive bolt stunning in alpacas (Vicugna pacos). Meat Science 100: 227231. https://doi.org/10.1016/j.meatsci.2014.10.022CrossRefGoogle ScholarPubMed
Gnjidić, Z, Kubat, M, Malenica, M, Sajko, T, Radić, I and Rumboldt, Z 2002 Epidemiological, forensic, clinical, and imaging characteristics of head injuries acquired in the suicide attempt with captive bolt gun. Acta Neurochirurgica 144: 12711277. https://doi.org/10.1007/s00701-002-1018-2CrossRefGoogle ScholarPubMed
Graham, DI and Gennarelli, TA 1997 Trauma. In: Graham, DI and Lantos, PL (eds) Greenfield's Neuropathology, Sixth Edition pp 197262. CRC Press: London, UKGoogle Scholar
Grandin, T 1980 Mechanical, electrical and anesthetic stunning methods for livestock. International Journal for the Study of Animal Problems 1: 242263Google Scholar
Grandin, T 1994 Euthanasia and slaughter of livestock. Journal of the American Veterinary Medical Association 204: 1354Google ScholarPubMed
Grandin, T 2002 Return-to-sensibility problems after penetrating captive bolt stunning of cattle in commercial beef slaughter plants. Journal of the American Veterinary Medical Association 221: 12581261. https://doi.org/10.2460/javma.2002.221.1258CrossRefGoogle ScholarPubMed
Gregory, N and Shaw, F 2000 Penetrating captive bolt stunning and exsanguination of cattle in abattoirs. Journal of Applied Animal Welfare Science 3: 215230. https://doi.org/10.1207/S15327604JAWS0303_3CrossRefGoogle Scholar
Gregory, NG 2007 Animal Welfare and Meat Production, Second Edition pp 191212. CABI: Wallingford, UK. https://doi.org/10.1079/9781845932152.0000CrossRefGoogle Scholar
Gregory, NG, Lee, CJ and Widdicombe, JP 2007 Depth of concussion in cattle shot by penetrating captive bolt. Meat Science 77: 499503. https://doi.org/10.1016/j.meatsci.2007.04.026CrossRefGoogle ScholarPubMed
Gregory, NG and Wotton, SB 1984 Sheep slaughtering procedures III. Head-to-back electrical stunning. British Veterinary Journal 140: 570575. https://doi.org/10.1016/0007-1935(84)90008-3CrossRefGoogle ScholarPubMed
Grellner, W, Buhmann, D and Wilske, J 2000 Suicide by double bolt gunshot wound to the head: case report and review of the lit-erature. Archiv Fur Kriminologie 205: 162168Google Scholar
Grist, A, Lines, J, Knowles, T, Mason, C and Wotton, S 2018a The use of a mechanical non-penetrating captive bolt device for the euthanasia of neonate lambs. Animals 8: 49. https://doi.org/10.3390/ani8040049CrossRefGoogle ScholarPubMed
Grist, A, Lines, J, Knowles, T, Mason, C and Wotton, S 2018b Use of a non-penetrating captive bolt for euthanasia of neonate goats. Animals 8: 58. https://doi.org/10.3390/ani8040058CrossRefGoogle Scholar
Grist, A, Lines, JA, Bock, R, Knowles, TG and Wotton, SB 2019 An examination of the performance of blank cartridges used in captive bolt devices for the pre-slaughter stunning and euthanasia of animals. Animals 9: 522. https://doi.org/10.3390/ani9080552CrossRefGoogle ScholarPubMed
Grist, A, Lines, JA, Knowles, TG, Mason, CW and Wotton, SB 2018c The use of a non-penetrating captive bolt for the euthanasia of neonate piglets. Animals 8: 117. https://doi.org/10.3390/ani8040048Google ScholarPubMed
Grist, A, Murrell, JC, McKinstry, JL, Knowles, TG and Wotton, SB 2017 Humane euthanasia of neonates I: validation of the effectiveness of the Zephyr EXL non-penetrating captive-bolt euthana-sia system on neonate piglets up to 10.9 kg live-weight. Animal Welfare 26: 111120. https://doi.org/10.7120/09627286.26.1.111CrossRefGoogle Scholar
Gurung, S, Hoffman, J, Stringfellow, K, Abi-Ghanem, D, Zhao, D, Caldwell, D, Lee, J, Styles, D, Berghman, L and Byrd, J 2018a Depopulation of caged layer hens with a compressed air foam system. Animals 8: 11. https://doi.org/10.3390/ani8010011CrossRefGoogle ScholarPubMed
Gurung, S, White, D, Archer, G, Styles, D, Zhao, D, Farnell, Y, Byrd, J and Farnell, M 2018b Carbon dioxide and nitrogen infused compressed air foam for depopulation of caged laying Hens. Animals 8: 6. https://doi.org/10.3390/ani8010006CrossRefGoogle ScholarPubMed
Haley, C, Dewey, CE, Widowski, T, Poljak, Z and Friendship, R 2008 Factors associated with in-transit losses of market hogs in Ontario in 2001. Canadian Journal of Veterinary Research 72: 377384Google ScholarPubMed
Hoenderken, R 1978 Electrical stunning of pigs for slaughter. Doctoral Thesis, University of Utrecht, The NetherlandsGoogle Scholar
Humane Slaughter Association (HSA) 2014 Humane killing of livestock using firearms pp 125. Humane Slaughter Association: Wheathampstead, UK. https://doi.org/10.1007/978-94-007-6167-4_377-1CrossRefGoogle Scholar
Janssen, W and Stieger, W 1964 Verletzungen durch Bolzenschuß-Apparate unter besonderer Berücksichtigung der Spurenmerkmale. Arch Kriminol 134: 2637. [Title translation: Injuries caused by nail guns with special consideration of trace features]Google Scholar
Kattimani, RP, Shetty, S and Mirza, H 2016 Accidental bolt gun injury to femur-A case report. Journal of Orthopaedic Case Reports 6: 6. https://doi.org/10.13107/jocr.2250-0685.544CrossRefGoogle ScholarPubMed
Kells, N, Beausoleil, N, Johnson, C and Sutherland, M 2018 Evaluation of different gases and gas combinations for on-farm euthanasia of pre-weaned pigs. Animals 8: 115. https://doi.org/10.3390/ani8030040CrossRefGoogle ScholarPubMed
Kline, HC, Wagner, DR, Edwards-Callaway, LN, Alexander, LR and Grandin, T 2019 Effect of captive bolt gun length on brain trauma and post-stunning hindlimb activity in finished cattle Bos taurus. Meat Science 155: 6973. https://doi.org/10.1016/j.meatsci.2019.05.004CrossRefGoogle ScholarPubMed
Lambooij, E, Gerritzen, MA, Engel, B, Hillebrand, SJW, Lankhaar, J and Pieterse, C 1999 Behavioural responses during exposure of broiler chickens to different gas mixtures. Applied Animal Behaviour Science 62: 255265. https://doi.org/10.1016/S0168-1591(98)00214-7CrossRefGoogle Scholar
Leach, MC, Bowell, VA, Allan, TF and Morton, DB 2002 Aversion to gaseous euthanasia agents in rats and mice. Comparative Medicine 52: 249257Google ScholarPubMed
Leach, MC, Bowell, VA, Allan, TF and Morton, DB 2004 Measurement of aversion to determine humane methods of anaesthesia and euthanasia. Animal Welfare 13: S77S86Google Scholar
Liotti, M, Brannan, S, Egan, G, Shade, R, Madden, L, Abplanalp, B, Robillard, R, Lancaster, J, Zamarripa, FE and Fox, PT 2001 Brain responses associated with consciousness of breathlessness (air hunger). Proceedings of the National Academy of Sciences 98: 20352040. https://doi.org/10.1073/pnas.98.4.2035CrossRefGoogle ScholarPubMed
Llonch, P, Dalmau, A, Rodríguez, P, Manteca, X and Velarde, A 2012 Aversion to nitrogen and carbon dioxide mixtures for stunning pigs. Animal Welfare 21: 3339. https://doi.org/10.7120/096272812799129475CrossRefGoogle Scholar
Llonch, P, Rodríguez, P, Casal, N, Carreras, R, Muñoz, I, Dalmau, A and Velarde, A 2015 Electrical stunning effectiveness with current levels lower than 1 A in lambs and kid goats. Research in Veterinary Science 98: 154161. https://doi.org/10.1016/j.rvsc.2014.12.009CrossRefGoogle ScholarPubMed
Marahrens, M, Pöhlmann, V, Balzer, K, Stehle, E, El Amarti, A, von Frantzius, I, Piotrowski, D, Kiezebrink, H and Schwarzlose, I 2017 Stunning of slaughter pigs and non-viable piglets with nitrogen gas filled high expansive foam - first results of a proof of concept. Welfare Impacts of Controlled Atmosphere Methods for Stunning or Killing Animals p 15. UFAW and HSA, Addlestone, UKGoogle Scholar
Martin, JE, Christensen, K, Vizzier-Thaxton, Y, Mitchell, M and McKeegan, DEF 2017 Low atmospheric pressure stunning in broilers: Temperature and illumination effects. Welfare impacts of con-trolled atmosphere methods for stunning or killing animals. UFAW and HSA Meeting. 13 December 2017, Reading, UK. https://doi.org/10.1080/00071668.2016.1201200CrossRefGoogle Scholar
Martoft, L, Stødkilde-Jørgensen, H, Forslid, A, Pedersen, HD and Jørgensen, PF 2003 CO2 induced acute respiratory acidosis and brain tissue intracellular pH: a 31 P NMR study in swine. Laboratory Animals 37: 241248. https://doi.org/10.1258/002367703766453092CrossRefGoogle ScholarPubMed
Matthis, JS 2005 Selected employee attributes and perceptions regarding methods and animal welfare concerns associated with swine euthanasia. Dissertation Thesis, North Carolina State University, USAGoogle Scholar
McKeegan, DEF, Reimert, HGM, Hindle, VA, Boulcott, P, Sparrey, JM, Wathes, CM, Demmers, TGM and Gerritzen, MA 2013a Physiological and behavioral responses of poultry exposed to gas-filled high expansion foam. Poultry Science 92: 11451154. https://doi.org/10.3382/ps.2012-02587CrossRefGoogle ScholarPubMed
McKeegan, DEF, Sandercock, DA and Gerritzen, MA 2013b Physiological responses to low atmospheric pressure stunning and the implications for welfare. Poultry Science 92: 858868. https://doi.org/10.3382/ps.2012-02749CrossRefGoogle ScholarPubMed
Millar, GI and Mills, DS 2000 Observations on the trajectory of the bullet in 15 horses euthanased by free bullet. The Veterinary Record 146: 754757. https://doi.org/10.1136/vr.146.26.754CrossRefGoogle ScholarPubMed
More, S, Bicout, D, Bøtner, A, Butterworth, A, Calistri, P, Depner, K, Edwards, S, Garin-Bastuji, B, Good, M and Gortazar Schmidt, C 2017 Low atmospheric pressure system for stunning broiler chickens. EFSA Journal 15: 186. https://doi.org/10.2903/j.efsa.2017.5056Google ScholarPubMed
Mores, MAZ and Mores, N 2014 Orientações para eutanásia de suínos em granjas pelo método de eletrocussão. Embrapa Suínos e Aves: Concórdia, Brazil. [Title translation: Guide for pigs’ euthana-sia using electrocution method, on-farm]Google Scholar
National Pork Board 2009 On-farm euthanasia of swine: Recommendations for the producer. Pub.04259-01/09. NPB: Des Moines, IA, USAGoogle Scholar
Nilsson, B and Nordström, CH 1977 Rate of cerebral energy consumption in concussive head injury in the rat. Journal of Neurosurgery 47: 274281. https://doi.org/10.3171/jns.1977.47.2.0274CrossRefGoogle ScholarPubMed
Oliveira, SEO 2017 Avaliação da eficiência da insensibilização de bovinos para o abate com uso de pistolas pneumáticas de dardo cativo. Universidade Estadual Paulista (UNESP): Jaboticabal, Sao Paulo, Brazil. [Title translation: Evaluation of the efficiency of stunning cattle for slaughter using pneumatic captive bolt guns]Google Scholar
Oliveira, SEO, Gregory, NG, Dalla Costa, FA, Gibson, TJ and Paranhos da Costa, MJR 2017 Efficiency of low versus high air-line pressure in stunning cattle with a pneumatically powered pen-etrating captive bolt gun. Meat Science 130: 6468. https://doi.org/10.1016/j.meatsci.2017.04.007CrossRefGoogle Scholar
Oliveira, SEO, Gregory, NG, Dalla Costa, FA, Gibson, TJ, Dalla Costa, OA and Paranhos da Costa, MJRP 2018 Effectiveness of pneumatically powered penetrating and non-pen-etrating captive bolts in stunning cattle. Meat Science 140: 913. https://doi.org/10.1016/j.meatsci.2018.02.010CrossRefGoogle ScholarPubMed
Ommaya, AK, Goldsmith, W and Thibault, L 2002 Biomechanics and neuropathology of adult and paediatric head injury. British Journal of Neurosurgery 16: 220242. https://doi.org/10.1080/02688690220148824CrossRefGoogle ScholarPubMed
Pearigen, P, Gwinn, R and Simon, RP 1996 The effects in vivo of hypoxia on brain injury. Brain Research 725: 184191. https://doi.org/10.1016/0006-8993(96)00215-6CrossRefGoogle ScholarPubMed
Purswell, JL, Thaxton, JP and Branton, SL 2007 Identifying process variables for a low atmospheric pressure stunning-killing system. Journal of Applied Poultry Research 16: 509513. https://doi.org/10.3382/japr.2007-00026CrossRefGoogle Scholar
Raj, ABM 1998 Changes in the somatosensory evoked potentials and spontaneous electroencephalogram of broiler chickens during exposure to gas mixtures. British Poultry Science 39: 686695. https://doi.org/10.1080/00071669888584CrossRefGoogle ScholarPubMed
Raj, ABM 1999 Behaviour of pigs exposed to mixtures of gases and the time required to stun and kill them: welfare implications. The Veterinary Record 144: 165168. https://doi.org/10.1136/vr.144.7.165CrossRefGoogle ScholarPubMed
Raj, ABM and Gregory, NG 1995 Welfare implications of the gas stunning of pigs 1. Determination of aversion to the initial inhalation of carbon dioxide or argon. Animal Welfare 4: 273280Google Scholar
Raj, ABM, Johnson, SP, Wotton, SB and McInstry, JL 1997 Welfare implications of gas stunning pigs: 3. The time to loss of somatosensory evoked potential and spontaneous electrocor-ticogram of pigs during exposure to gases. The Veterinary Journal 153: 329339. https://doi.org/10.1016/S1090-0233(97)80067-6CrossRefGoogle ScholarPubMed
Raj, M 2008a Humane killing of nonhuman animals for disease control purposes. Journal of Applied Animal Welfare Science 11: 112124. https://doi.org/10.1080/10888700801925679CrossRefGoogle ScholarPubMed
Raj, MAB 2008b Welfare of pigs during stunning and slaughter. In: Faucitano, L and Schaeffer, AL (eds) The Welfare of Pigs: From Birth to Slaughter, First Edition pp 225240. Wageningen Academic Publishers: The NetherlandsGoogle Scholar
Rault, J-L, Holyoake, T and Coleman, G 2017 Stockperson atti-tudes toward pig euthanasia. Journal of Animal Science 95: 949957. https://doi.org/10.2527/jas.2016.0922Google Scholar
Rault, J-L, Kells, N, Johnson, C, Dennis, R, Sutherland, M and Lay, DC Jr 2015 Nitrous oxide as a humane method for piglet euthanasia: Behavior and electroencephalography (EEG). Physiology & Behavior 151: 2937. https://doi.org/10.1016/j.physbeh.2015.06.026CrossRefGoogle ScholarPubMed
Rault, J-L, McMunn, KA, Marchant-Forde, JN and Lay, DC Jr 2013 Gas alternatives to carbon dioxide for euthanasia: a piglet perspective. Journal of Animal Science 91: 18741883. https://doi.org/10.2527/jas.2012-5761CrossRefGoogle ScholarPubMed
Rawnsley, K 1985 Helping the sick doctor: a new service. British Medical Journal 291: 922. https://doi.org/10.1136/bmj.291.6500.922CrossRefGoogle Scholar
Ring, C, Erhardt, W, Kraft, H, Schmid, A, Weinmann, HM, Berner, H and Unshelm, J 1988 CO2 Anaesthesia for slaughter pigs. Fleischwirtschaft 68: 14781484. https://doi.org/10.3382/ps.0681478Google Scholar
Sadler, LJ, Hagen, CD, Wang, C, Widowski, TM, Johnson, AK and Millman, ST 2013 Comparison of CO2 versus mixed CO2: Argon gas at different flow rates using the smart box euthanasia device as an effective method of piglet euthanasia. Animal Industry Report 659: 76. https://doi.org/10.31274/ans_air-180814-679Google Scholar
Sadler, LJ, Hagen, CD, Wang, C, Widowski, TM, Johnson, AK and Millman, ST 2014 Effects of flow rate and gas mixture on the welfare of weaned and neonate pigs during gas euthanasia. Journal of Animal Science 92: 793805. https://doi.org/10.2527/jas.2013-6598CrossRefGoogle ScholarPubMed
Sandercock, DA, Auckburally, A, Flaherty, D, Sandilands, V and McKeegan, DEF 2014 Avian reflex and electroencephalo-gram responses in different states of consciousness. Physiology & Behavior 133: 252259. https://doi.org/10.1016/j.physbeh.2014.05.030CrossRefGoogle Scholar
Schiffer, KJ, Retz, SK, Richter, U, Algers, B and Hensel, O 2014 Assessment of key parameters for gunshot used on cattle: a pilot study on shot placement and effects of diverse ammunition on isolated cattle heads. Animal Welfare 23: 479489. https://doi.org/10.7120/09627286.23.4.479CrossRefGoogle Scholar
Sharp, TM, McLeod, SR, Leggett, KEA and Gibson, TJ 2015 Evaluation of a spring-powered captive bolt gun for killing kanga-roo pouch young. Wildlife Research 41: 623632. https://doi.org/10.1071/WR14094CrossRefGoogle Scholar
Shaw, NA 2002 The neurophysiology of concussion. Progress in Neurobiology 67: 281344. https://doi.org/10.1016/S0301-0082(02)00018-7CrossRefGoogle ScholarPubMed
Simic, M, Draskovic, D, Stojiljkovic, G, Vukovic, R and Budimlija, ZM 2007 The characteristics of head wounds inflicted by ‘humane killer’ (captive-bolt gun). A 15-year study. Journal of Forensic Sciences 52: 11821185. https://doi.org/10.1111/j.1556-4029.2007.00519.xCrossRefGoogle Scholar
Smith, DC 1965 Methods of euthanasia and disposal of laborato-ry animals. In: Gay, WI (ed) Methods of Animal Experimentation, First Edition pp 167195. Academic Press: New York, USA. https://doi.org/10.1016/B978-1-4832-3220-1.50012-7CrossRefGoogle Scholar
Smith, RK, Rault, J-L, Gates, RS and Lay, DC 2018 A two-step process of nitrous oxide before carbon dioxide for humanely euthanizing piglets: on-farm trials. Animals 8: 52. https://doi.org/10.3390/ani8040052CrossRefGoogle ScholarPubMed
Svendsen, O, Jensen, SK, Karlsen, LV, Svalastoga, E and Jensen, HE 2008 Observations on newborn calves rendered unconscious with a captive bolt gun. The Veterinary Record 162: 9092. https://doi.org/10.1136/vr.162.3.90Google ScholarPubMed
Sweetman, SC 2012 Martindale: The Complete Drug Reference. Pharmaceutical Press: London, UKGoogle Scholar
Thomson, DU, Wileman, BW, Rezac, DJ, Miesner, MD, Johnson-Neitman, JL and Biller, DS 2013 Computed tomo-graphic evaluation to determine efficacy of euthanasia of yearling feedlot cattle by use of various firearm-ammunition combinations. American Journal of Veterinary Research 74: 13851391. https://doi.org/10.2460/ajvr.74.11.1385CrossRefGoogle Scholar
Thürauf, N, Günther, M, Pauli, E and Kobal, G 2002 Sensitivity of the negative mucosal potential to the trigeminal target stimulus CO2. Brain Research 942: 7986. https://doi.org/10.1016/S0006-8993(02)02697-5CrossRefGoogle Scholar
Tordrup, PJ and Kjeldsen, SR 1994 Accidental injuries from captive-bolt guns (slaughterer's gun). Injury 25: 497499. https://doi.org/10.1016/0020-1383(94)90088-4CrossRefGoogle ScholarPubMed
Van Liere, EJ 1943 Anoxia. Its effect on the body. The American Journal of the Medical Sciences 205: 433. https://doi.org/10.1097/00000441-194303000-00027CrossRefGoogle Scholar
Velarde, A and Raj, M 2016a Mechanical stunning and killing methods. In: Velarde, A and Raj, M (eds) Animal Welfare at Slaughter, First Edition pp 91110. 5m Publishing: Sheffield, UKGoogle Scholar
Velarde, A and Raj, M 2016b Gas stunning and killing methods. In: Velarde, A and Raj, M (eds) Animal Welfare at Slaughter, First Edition pp 133151. 5m Publishing: Sheffield, UKGoogle Scholar
Verhoeven, M, Gerritzen, M, Velarde, A, Hellebrekers, L and Kemp, B 2016 Time to loss of consciousness and its relation to behavior in slaughter pigs during stunning with 80 or 95% carbon dioxide. Frontiers in Veterinary Science 3: 38. https://doi.org/10.3389/fvets.2016.00038CrossRefGoogle ScholarPubMed
Viel, G, Schröder, AS, Püschel, K and Braun, C 2009 Planned complex suicide by penetrating captive-bolt gunshot and hanging: case study and review of the literature. Forensic Science International 187: e7–e11. https://doi.org/10.1016/j.forsciint.2009.01.022CrossRefGoogle Scholar
Viola, L, Costantinides, F, Di Nunno, C, Battista, GM and Di Nunno, N 2004 Suicide with a butcher's bolt. Journal of Forensic Science 49: JFS20030243. https://doi.org/10.1520/JFS2003024CrossRefGoogle ScholarPubMed
Vizzier-Thaxton, Y, Christensen, KD, Schilling, MW, Buhr, RJ and Thaxton, JP 2010 A new humane method of stunning broil-ers using low atmospheric pressure. Journal of Applied Poultry Research 19: 341348. https://doi.org/10.3382/japr.2010-00184CrossRefGoogle Scholar
Wagner, DR, Kline, HC, Martin, MS, Alexander, LR, Grandin, T and Edwards-Callaway, LN 2019 The effects of bolt length on penetration hole characteristics, brain damage and specified-risk material dispersal infinished cattle stunned with a penetrating captive bolt stunner. Meat Science 155: 109114. https://doi.org/10.1016/j.meatsci.2019.05.006CrossRefGoogle ScholarPubMed
Walsh, JL, Percival, A and Turner, PV 2017 Efficacy of blunt force trauma, a novel mechanical cervical dislocation device, and a non-penetrating captive bolt device for on-farm euthanasia of pre-weaned kits, growers, and adult commercial meat rabbits. Animals 7: 100. https://doi.org/10.3390/ani7120100Google Scholar
Whiting, TL, Steele, GG, Wamnes, S and Green, C 2011 Evaluation of methods of rapid mass killing of segregated early weaned piglets. The Canadian Veterinary Journal 52: 753758Google ScholarPubMed
Widowski, T, Elgie, R and Lawlis, P 2008 Assessing the effective-ness of a non-penetrating captive bolt for euthanasia of newborn piglets. Allen D Leman Swine Conference Proceedings pp 107111. University of Minnesota Digital Conservancy, USAGoogle Scholar
Woods, J, Shearer, JK and Hill, J 2010 Recommended on-farm euthanasia practices. In: Grandin, T (ed) Improving Animal Welfare: A Practical Approach, Second Edition pp 186201. CABI: Wallingford, UKGoogle Scholar
Wotton, SB 1996 New advances in stunning techniques for slaughter animals. Meat Focus International 12: 461465Google Scholar
Young, RJ and Destian, S 2002 Imaging of traumatic intracranial hemorrhage. Neuroimaging Clinics 12: 189204. https://doi.org/10.1016/S1052-5149(02)00003-5CrossRefGoogle ScholarPubMed
Supplementary material: File

Dalla Costa et al. supplementary material
Download undefined(File)
File 244.9 KB