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Housing and Welfare in Laboratory Rats: Welfare Implications of Isolation and Social Contact Among Caged Males

Published online by Cambridge University Press:  11 January 2023

J L Hurst ,
C J Barnard ,
C M Nevison  and
C D West
J L Hurst*
Affiliation:
Behaviour and Ecology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
C J Barnard
Affiliation:
Behaviour and Ecology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
C M Nevison
Affiliation:
Behaviour and Ecology Research Group, Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK
C D West
Affiliation:
Central Toxicology Laboratory, Zeneca PLC, Alderley Park, Macclesfield, Cheshire, SK10 4TJ, UK
*
† Contact for correspondence and requests for reprints
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Abstract

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Male laboratory rats (Rattus norvegicus; Wistar, Alderley Park) were housed as singletons or groups of three in units of two joined, but divided cages. Units were divided by different types of barrier that allowed different degrees of social contact across the barrier. Singletons were established either with another singleton as a neighbour on the other side of the barrier, or with a group of three as neighbours. Relative to group-housed animals, singly-housed rats showed reduced activity and a greater incidence of self-directed behaviours and behaviours apparently related to escape or seeking social information. Pathophysiological evidence was consistent with Baenninger's (1967) suggestion that tail manipulation in singletons is a surrogate social response, but was also consistent with an overall increase in self-directed activity, reflecting elasticity in time budgeting. Variation in the degree of increase in self-directed activity among singletons and the negative correlation between self-directed activity and organ pathology may have reflected differences in the ability of individuals to avoid an activity limbo. While reduced corticosterone concentration and organ pathology compared with grouped rats implied that separation may remove social stress, responses to contact with neighbours, and correlations between behaviours and organ pathology suggested that rats may actively seek social interaction. Broad differences in stress responses between single and grouped housing conditions may therefore be an inadequate yardstick to the animals’ welfare. However, exposure to neighbours reduced the aggressiveness of singly-housed males when they were eventually introduced into an unfamiliar group, suggesting that a degree of exposure to neighbours (separation, but not isolation) may have some welfare benefits for laboratory-housed rats, depending on procedures.

Keywords

animal welfareisolationpathophysiologyratsocial stresstime budget
Type
Research Article
Information
Animal Welfare , Volume 6 , Issue 4 , November 1997 , pp. 329 - 347
Copyright
© 1997 Universities Federation for Animal Welfare

References

Baenninger, L P 1967 Comparison of behavioural development in socially isolated and grouped rats. Animal Behaviour 15: 312–323CrossRefGoogle ScholarPubMed
Barnett, S A 1975 The Rat: A Study in Behaviour. University of Chicago Press: Chicago, USAGoogle Scholar
Barnard, C J, Behnke, J M and Sewell, J 1993 Social behaviour, stress and susceptibility to infection in house mice (Mus musculus): effects of duration of grouping and aggressive behaviour prior to infection on susceptibility to Babesia microti. Parasitology 107: 183–192CrossRefGoogle Scholar
Barnard, C J, Behnke, J M and Sewell, J 1996 Social status and resistance to disease in house mice (Mus musculus): status-related hormonal responses in relation to immunity costs in different social and physical environments. Ethology 102: 63–84CrossRefGoogle Scholar
Barnard, C J and Hurst, J L 1996 Welfare by design: the natural selection of welfare criteria. Animal Welfare 5: 405–433CrossRefGoogle Scholar
Beynen, A C 1992 Communication between rats of experiment-induced stress and its impact on experimental results. Animal Welfare 1: 153–159CrossRefGoogle Scholar
Brain, P F and Nowell, N W 1970 The effects of differential grouping on endocrine function of male albino mice. Physiology and Behavior 5: 907–910CrossRefGoogle ScholarPubMed
Brain, P F, Benton, D, Howell, P A and Jones, S E 1980 Resident rats’ aggression towards intruders. Animal Learning and Behaviour 8: 331–335CrossRefGoogle Scholar
Brain, P F and Benton, D 1983 Conditions of housing, hormones, and aggressive behavior. In: Svare, B B (ed) Hormones and Aggressive Behavior, pp 351–372. Plenum Press: New York, USACrossRefGoogle Scholar
Cairns, R B, Hood, K E and Midlairn, J 1985 On fighting in mice: is there a sensitive period for isolation? Animal Behaviour 33: 166–80CrossRefGoogle Scholar
Carlier, P G, Crine, A F, Yerna, N M and Rorive, G L 1988 Cardiovascular structural changes induced by isolation-stress hypertension in the rat. Journal of Hypertension 6(supplement 4): 112–115CrossRefGoogle ScholarPubMed
Döhler, K D, Gartner, K, Mühlen, A V and Döhler, U 1977 Activation of anterior pituitary, thyroid and adrenal glands in rats after disturbance stress. Acta Endocrinologica, Copenhagen 86: 489–97Google ScholarPubMed
Edwards, E A, Rahe, R H, Stephens, P M and Henry, J P 1980 Antibody responses to bovine serum albumin in mice: the effects of psychosocial environmental change. Proceedings of the Society for Experimental Biology and Medicine 164: 478–481CrossRefGoogle Scholar
Ehlers, C L, Kaneko, W M, Owens, M J and Nemeroff, C B 1993 Effects of gender and social isolation on electroencephalogram and neuroendocrine parameters in rats. Biological Psychiatry 33: 358–366CrossRefGoogle ScholarPubMed
Fägerstam L G, Frostell-Karlsson Å, Karlsson R, Persson B and Rönnberg 1 1992 Biospeeife interaction analysis using surface plasmon resonance detection applied to kinetic, binding site and concentration analysis. Journal of Chromatography 597: 397–410CrossRefGoogle Scholar
Falk, J L 1971 The nature and determinants of adjunctive behavior. Physiology and Behavior 6: 577–588CrossRefGoogle ScholarPubMed
Fiala, B, Snow, F M and Greenough, W T 1977 ‘Impoverished’ rats weigh more than ‘enriched’ rats because they eat more. Developmental Psychobiology 10: 537–541CrossRefGoogle ScholarPubMed
Fredericson, E 1953 The wall-seeking tendency of three inbred mouse strains (Mas musculus). Journal of General Psychology 82: 143–146Google Scholar
Friedman, L, Glasgow, A and Adder, R 1970 Differential susceptibility to a viral agent in mice housed alone or in groups. Psychosomatic Medicine 32: 285–290CrossRefGoogle ScholarPubMed
Friend, T H, Polan, C E, Gwazdauskas, F C and Heald, C W 1977 Adrenal glucocorticoid response to exogenous adrenocorticotropin mediated by density and social disruption in lactating cows. Journal of Dairy Science 60: 1958–1963CrossRefGoogle ScholarPubMed
Gardiner, S M and Bennett, T 1997 The effects of short-term isolation on systolic blood pressure and heart rate in rats. Medicine & Biology 55: 325–329Google Scholar
Garzon, J and Del Rio, J 1981 Hyperactivity induced in rats by long-term isolation: further studies on a new animal model for the detection of antidepressants. European Journal of Pharmacology 74: 278–294CrossRefGoogle ScholarPubMed
Grant, E C 1963 An analysis of the social behaviour of the male laboratory rat. Behaviour 21: 260–281CrossRefGoogle Scholar
Grossman, C J 1985 Interactions between the gonadal steroids and the immune system. Science 277: 257–261CrossRefGoogle Scholar
Hurst, J L, Barnard, C J, Hare, R, Wheeldon, E B and West, C D 1996 Housing and welfare in laboratory rats: status-dependent time-budgeting and pathophysiology in single sex groups maintained in open rooms. Animal Behaviour 52: 335–360CrossRefGoogle Scholar
Lore, R L and Flannelly, K 1977 Rat societies. Scientific American 236: 106–116CrossRefGoogle ScholarPubMed
McFarland, D J 1989 Problems of Animal Behaviour. Longman: London, UKGoogle Scholar
Manser, C E 1992 The Assessment of Stress in Laboratory Animals. Royal Society for the Protection of Animals: Horsham, UKGoogle Scholar
Mason, G J 1991 Stereotypies: a critical review. Animal Behaviour 41: 1015–1038.CrossRefGoogle Scholar
Mason, G J and Mendl, M 1993 Why is there no simple way of measuring animal welfare? Animal Welfare 2: 301–319CrossRefGoogle Scholar
Mendl, M 1991 Some problems with the concept of a cut-off point for determining when an animal’s welfare is at risk. Applied Animal Behaviour Science 31: 139–146CrossRefGoogle Scholar
Morgan, M J and Einon, D F 1976 Activity and exploration in thyroid deficient and socially-isolated rats. Physiology and Behavior 16: 107–110CrossRefGoogle ScholarPubMed
Morinan, A and Leonard, B E 1980 Some anatomical and physiological correlates of social isolation in the young rat. Physiology and Behavior 24: 637–640CrossRefGoogle ScholarPubMed
Mormède, P, Lemaire, V, Castanon, N, Dulluc, J, Laval, M M and Le Moal, M 1990 Multiple neuroendocrine responses to chronic social stress: interaction between individual and situational factors. Physiology and Behavior 47: 1099–1105CrossRefGoogle Scholar
Mugford, R A and Nowell, N W 1971 The preputial glands as a source of aggression promoting odors in mice. Physiology and Behavior 6: 247–249CrossRefGoogle ScholarPubMed
Pitman, D L, Otterweller, J E and Natelson, B H 1990 Effect of stressor intensity on habituation and sensitization of glucocorticoid responses in rats. Behavioral Neuroscience 104: 28–36CrossRefGoogle ScholarPubMed
Rabin, B S, Lyte, M and Hamill, E 1987 The influence of mouse strain and housing on the immune response. Journal of Neuroimmunology 17: 11–16CrossRefGoogle ScholarPubMed
Restrepo, C and Armorio, A 1987 Chronic stress alters pituitary-adrenal function of prepubertal male rats. Psychoneuroendocrinology 12: 393–395CrossRefGoogle ScholarPubMed
Rushen, J and de Passille, A M B 1992 The scientific assessment of the impact of housing on animal welfare. Applied Animal Behaviour Science 28: 381–386CrossRefGoogle Scholar
Sapolsky, R M 1983 Individual differences in Cortisol secretory patterns in the wild baboon: role of negative feedback sensitivity. Endocrinology 113: 2263–2267CrossRefGoogle ScholarPubMed
Tuli, J S, Smith, J A and Morton, D B 1994 Corticosterone, adrenal and spleen weight in mice after tail bleeding, and its effect on nearby animals. Laboratory Animals 29: 90–95CrossRefGoogle Scholar
Valzelli, L and Bernassconi, S 1976 Psychoactive drug effect on behavioural changes induced by prolonged socio-environmental deprivation in rats. Physics in Medicine and Biology 6: 271–276Google ScholarPubMed
Wahid, F N and Behnke, J M 1993 Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): parasite specific IgGl antibody response and primary response phenotype. Parasite Immunology 15: 401–413CrossRefGoogle Scholar
Wiepkema, P R and Koolhaas, J M 1993 Stress and animal welfare. Animal Welfare 2: 195–218CrossRefGoogle Scholar
Wolfensohn, S and Lloyd, M 1994 Handbook of Laboratory Animal Management and Welfare. Oxford University Press: Oxford, UKGoogle Scholar