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Hang on: an evaluation of the hemp rope as environmental enrichment in C57BL/6 mice

Published online by Cambridge University Press:  01 January 2023

K Gjendal ,
DB Sørensen ,
MK Kiersgaad  and
JL Ottesen
K Gjendal*
Affiliation:
Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Måløv, Denmark
DB Sørensen
Affiliation:
Section of Experimental Animal Models, Faculty of Health and Medical Sciences, University of Copenhagen, Groennegaardsvej 15, DK-1870 Frederiksberg C, Denmark
MK Kiersgaad
Affiliation:
Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Måløv, Denmark
JL Ottesen
Affiliation:
Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Måløv, Denmark
*
* Contact for correspondence and requests for reprints: [email protected]
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Abstract

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When introducing environmental enrichment in laboratory animals, positive and/or negative effects on behavioural and physiological parameters should be evaluated. This three-step randomised controlled trial in male C57BL/6 mice investigated the effect of supplementing the environment with one or more hemp ropes. In part 1, the effect of a hemp rope on aggressive and social behaviour, stress and anxiety levels was assessed by social interaction test, elevated plus maze behaviour, and faecal corticosterone metabolites, respectively (n = 224 mice). In part 2, the effect of 1, 2 or 7 hemp ropes on aggressive behaviour in mice subjected to routine handling was evaluated by assessing the number of wounded companion animals and wounds per animal (n = 224). In part 3, climbing activity in the rope and amount of material shredded from the rope was assessed (n = 56). Mice housed with one hemp rope engaged in social behaviour for longer time than mice housed without a hemp rope, while no difference was detected in stress and anxiety levels. No difference was seen in the number of wounded animals or wounds per animal when adding 1, 2 or 7 hemp ropes to the existing environment in mice undergoing minimal human handling. The mice continuously shredded and climbed the rope, even when provided with a new rope, although time spent climbing decreased slightly over time. Thus, a hemp rope can be used as additional environmental enrichment amongst male C57BL/6 mice.

Keywords

aggressionanimal welfareenvironmental enrichmentmicestresswounds
Type
Research Article
Information
Animal Welfare , Volume 26 , Issue 4 , November 2017 , pp. 437 - 447
Copyright
© 2017 Universities Federation for Animal Welfare

References

Abou-Ismail, UA 2011 The effects of cage enrichment on ago-nistic behaviour and dominance in male laboratory rats (Rattus norvegicus). Research in Veterinary Science 90: 346–351. https://doi.org/10.1016/j.rvsc.2010.06.010CrossRefGoogle Scholar
Akre, AK, Bakken, M, Hovland, AL, Palme, R and Mason, G 2011 Clustered environmental enrichments induce more aggression and stereotypic behaviour than do dispersed enrichments in female mice. Applied Animal Behaviour Science 131: 145–152. https://doi.org/10.1016/j.applanim.2011.01.010CrossRefGoogle Scholar
Ambrose, N and Morton, DB 2000 The use of cage enrichment to reduce male mouse aggression. Journal of Applied Animal Welfare Science 3(2): 117–125. https://doi.org/10.1207/S15327604 JAWS0302_4Google Scholar
Balcombe, J 2010 Laboratory rodent welfare: thinking outside the cage. Journal of Applied Animal Welfare Science 13: 77–88. https://doi.org/10.1080/10888700903372168CrossRefGoogle ScholarPubMed
Balcombe, JP 2006 Laboratory environments and rodents’ behavioural needs: a review. Laboratory Animals 40: 217–235. https://doi.org/10.1258/002367706777611488CrossRefGoogle ScholarPubMed
Baumans, V 2005 Environmental enrichment for laboratory rodents and rabbits: requirements of rodents, rabbits, and research. ILAR Journal/National Research Council, Institute of Laboratory Animal Resources 46: 162–170. https://doi.org/10.1093/ilar.46.2.162CrossRefGoogle ScholarPubMed
Baumans, V and van Loo, PLP 2013 How to improve housing conditions of laboratory animals: the possibilities of environmen-tal refinement. Veterinary Journal 195: 24–32. https://doi.org/10.1016/j.tvjl.2012.09.023CrossRefGoogle Scholar
Berry, RJ 1970 The natural history of the house mouse. Field Studies 3: 219–262Google Scholar
Blanchard, DC and Blanchard, RJ 2005 Stress and aggressive behaviours. In: Nelson, RJ (ed) Biology of Aggression. Oxford Scholarship Online. http: 1093/acprof:oso/9780195168761.003.0012Google Scholar
Blanchard, RJ, O’Donnell, V and Blanchard, DC 1979 Attack and defensive behaviors in the albino mouse. Aggressive Behavior 8: 341–352. https://doi.org/10.1002/1098-2337(1979)5:4<341::AID-AB2480050403>3.0.CO;2-H3.0.CO;2-H>CrossRefGoogle Scholar
Directive 2010/63/EU 2010 Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the Protection of Animals used for Scientific Purposes. EU: Brussels, BelgiumGoogle Scholar
File, SE and Seth, P 2003 A review of 25 years of the social interaction test. European Journal of Pharmacology 463: 35–53. https://doi.org/10.1016/S0014-2999(03)01273-1CrossRefGoogle ScholarPubMed
Fox, C, Merali, Z and Harrison, C 2006 Therapeutic and pro-tective effect of environmental enrichment against psychogenic and neurogenic stress. Behavioural Brain Research 175: 1–8. https://doi.org/10.1016/j.bbr.2006.08.016CrossRefGoogle Scholar
Gouveia, K and Hurst, JL 2013 Reducing mouse anxiety during handling: effect of experience with handling tunnels. PloS One 8: e66401. https://doi.org/10.1371/journal.pone.0066401CrossRefGoogle ScholarPubMed
Haemisch, A and Gärtner, K 1994 The cage design affects intermale aggression in small groups of male laboratory mice: strain specific consequences on social organization, and endocrine activations in two inbred strains (DBA/2J and CBA/J). Journal of Experimental Animal Science 36: 101–116Google Scholar
Haemisch, A and Gärtner, K 1997 Effects of cage enrichment on territorial aggression and stress physiology in male laborato-ry mice. Acta physiologica Scandinavica Supplementum 640: 73–76Google Scholar
Haemisch, A, Voss, T and Gärtner, K 1994a Effects of envi-ronmental enrichment on aggressive behavior, dominance hierar-chies, and endocrine states in male DBA/2J mice. Physiology and Behavior 56: 1041–1048. https://doi.org/10.1016/0031-9384(94)90341-7CrossRefGoogle Scholar
Haemisch, A, Voss, T and Gärtner, K 1994b Effects of envi-ronmental enrichment on aggressive behavior, dominance hierar-chies, and endocrine states in male DBA/2J mice. Physiology and Behavior 56: 1041–1048. https://doi.org/10.1016/0031-9384(94)90341-7CrossRefGoogle Scholar
Hurst, JL and West, RS 2010 Taming anxiety in laboratory mice. Nature Methods 7: 825–826. https://doi.org/10.1038/nmeth.1500CrossRefGoogle ScholarPubMed
Hutchinson, EK, Avery, A and Vandewoude, S 2005 Environmental enrichment for laboratory rodents. Institute for Laboratory Animal Research Journal 46(2): 148–161. https://doi.org/10.1093/ilar.46.2.148Google ScholarPubMed
Hutchinson, EK, Avery, AC and Vandewoude, S 2012 Environmental enrichment during rearing alters corticosterone levels, thymocyte numbers, and aggression in female BALB/c mice. Journal of the American Association for Laboratory Animal Science 51: 18–24Google ScholarPubMed
Kaliste, EK, Mering, SM and Huuskonen, HK 2006 Environmental modification and agonistic behavior in NIH/S male mice: nesting material enhances fighting but shelters prevent it. Comparative Medicine 56: 202–208Google Scholar
Kilkenny, C, Browne, WJ, Cuthill, IC, Emerson, M and Altman, DG 2010 Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biology 8: e1000412. https://doi.org/10.1371/journal.pbio.1000412CrossRefGoogle ScholarPubMed
Latham, N and Mason, G 2004 From house mouse to mouse house: the behavioural biology of free-living Mus musculus and its implications in the laboratory. Applied Animal Behaviour Science 86: 261–289. https://doi.org/10.1016/j.applanim.2004.02.006CrossRefGoogle Scholar
Litvin, Y, Blanchard, DC, Pentkowski, NS and Blanchard, RJ 2007 A pinch or a lesion: a reconceptualization of biting con-sequences in mice. Aggressive Behavior 33: 545–551. https://doi.org/10.1002/ab.20222CrossRefGoogle ScholarPubMed
Mackintosh, JH 1973 Factors affecting the recognition of terri-tory boundaries by mice (Mus musculus). Animal Behaviour 21:464–470. https://doi.org/10.1016/S0003-3472(73)80006-5CrossRefGoogle Scholar
Marashi, V, Barnekow, A, Ossendorf, E and Sachser, N 2003 Effects of different forms of environmental enrichment on behav-ioral, endocrinological, and immunological parameters in male mice. Hormones and Behavior 43: 281–292. https://doi.org/10.1016/S0018-506X(03)00002-3CrossRefGoogle Scholar
Marashi, V, Barnekow, A and Sachser, N 2004 Effects of envi-ronmental enrichment on males of a docile inbred strain of mice. Physiology and Behavior 82: 765–776. https://doi.org/10.1016/j.physbeh.2004.05.009CrossRefGoogle Scholar
McQuaid, RJ, Audet, M-C and Anisman, H 2012 Environmental enrichment in male CD-1 mice promotes aggres-sive behaviors and elevated corticosterone and brain norepi-nephrine activity in response to a mild stressor. Stress 15: 354–360. https://doi.org/10.3109/10253890.2011.623249CrossRefGoogle Scholar
Mesa-Gresa, P, Pérez-Martinez, A and Redolat, R 2013 Environmental enrichment improves novel object recognition and enhances agonistic behavior in male mice. Aggressive Behavior 39: 269–279. https://doi.org/10.1002/ab.21481CrossRefGoogle ScholarPubMed
Möstl, E and Palme, R 2002 Hormones as indicators of stress. Domestic Animal Endocrinology 23: 67–74. https://doi.org/10.1016/S0739-7240(02)00146-7CrossRefGoogle ScholarPubMed
Olsson, IAS and Dahlborn, K 2002 Improving housing conditions for laboratory mice: a review of ‘environmental enrich-ment’. Laboratory Animals 36: 243–270. https://doi.org/10.1258/002367702320162379Google Scholar
Palme, R, Rettenbacher, S, Touma, C, El-Bahr, SM and Möstl, E 2005 Stress hormones in mammals and birds: compar-ative aspects regarding metabolism, excretion, and non-invasive measurement in fecal samples. Annals of the New York Academy of Sciences 1040: 162–171. https://doi.org/10.1196/annals.1327.021CrossRefGoogle Scholar
Roy, V, Belzung, C, Delarue, C and Chapillon, P 2001 Environmental enrichment in BALB/c mice: effects in classical tests of anxiety and exposure to a predatory odor. Physiology and Behavior 74: 313–320. https://doi.org/10.1016/S0031-9384(01)00561-3Google ScholarPubMed
Sørensen, D, Ottesen, J and Hansen, A 2004 Consequences of enhancing environmental complexity for laboratory rodents: a review with emphasis on the rat. Animal Welfare 13: 193–204Google Scholar
Sundbom, R, Jacobsen, KR, Kalliokoski, O, Hau, J and Abelson, KSP 2011 Post-operative corticosterone levels in plas-ma and feces of mice subjected to permanent catheterization and automated blood sampling. In Vivo 25: 335–342Google Scholar
van de Weerd, HA, Aarsen, EL, Mulder, A, Kruitwagen, CLJJ, Hendriksen, CFM and Baumans, V 2002 Effects of envi-ronmental enrichment for mice: variation in experimental results. Journal of Applied Animal Welfare Science 5: 87–109. https://doi.org/10.1207/S15327604JAWS0502_01CrossRefGoogle Scholar
van de Weerd, HA, van Loo, PL, van Zutphen, LF, Koolhaas, JM and Baumans, V 1997 Nesting material as envi-ronmental enrichment has no adverse effects on behavior and physiology of laboratory mice. Physiology and Behavior 62: 1019–1028. https://doi.org/10.1016/S0031-9384(97)00232-1CrossRefGoogle Scholar
van Loo, PLP, Kruitwagen, CLJJ, Koolhaas, JM, van de Weerd, HA, van Zutphen, LF and Baumans, V 2002 Influence of cage enrichment on aggressive behaviour and physi-ological https://doi.org/10.1016/S0168-1591(01)00200-3CrossRefGoogle Scholar
von Holst, D 1998 The concept of stress and its relevance for animal behavior. Advances in the Study of Behavior 27: 1–131. https://doi.org/10.1016/S0065-3454(08)60362-9CrossRefGoogle Scholar
Walf, AA and Frye, CA 2007 The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nature Protocols 2: 322–328. https://doi.org/10.1038/nprot.2007.44CrossRefGoogle ScholarPubMed
Whitten, PL, Brockman, DK and Stavisky, RC 1998 Recent advances in noninvasive techniques to monitor hormone-behavior interactions. American Journal of Physical Anthropology 27(S): 1–233.0.CO;2-H>CrossRefGoogle ScholarPubMed
Würbel, H and Garner, JP 2007 Refinement of rodent research through environmental enrichment and systematic randomization. NC3Rs. www.nc3rs.org.ukGoogle Scholar