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Assessment of aversion and unconsciousness during exposure to carbon dioxide at high concentration in lambs

Published online by Cambridge University Press:  01 January 2023

P Rodríguez ,
A Dalmau ,
X Manteca ,
H Litvan ,
EW Jensen  and
A Velarde
P Rodríguez
Affiliation:
IRTA, Animal Welfare Subprogram, IRTA, Veïnat de Sies, s/n I7I2I, Monells, Spain UAB, School of Veterinary Science, 08193 Bellaterra, Spain
A Dalmau
Affiliation:
IRTA, Animal Welfare Subprogram, IRTA, Veïnat de Sies, s/n I7I2I, Monells, Spain
X Manteca
Affiliation:
UAB, School of Veterinary Science, 08193 Bellaterra, Spain
H Litvan
Affiliation:
UAB, Department of Cardiac Anaesthesia and Postoperative Intensive Care, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain
EW Jensen
Affiliation:
Center for Research in Biomedical Engineering, Technical University of Catalonia, Barcelona, Spain
A Velarde*
Affiliation:
IRTA, Animal Welfare Subprogram, IRTA, Veïnat de Sies, s/n I7I2I, Monells, Spain
*
* Contact for correspondence and requests for reprints: [email protected]
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Abstract

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The most widely used stunning method in sheep is electrical. However, in lambs, this method leads to rupture of the blood vessels, provoking ecchymoses. In pigs (Sus scrofa), the use of CO2-stunning systems has increased in popularity due to positive effects on meat quality and animal welfare (movement of animals in groups). The aim here was to assess the effectiveness of a progressive exposure to 90% carbon dioxide (CO2) in inducing unconsciousness in lambs (Ovis aries) through changes in the middle latency auditory-evoked potentials (MLAEP) of the central nervous system (CNS), blood parameters (pH, carbon dioxide partial pressure (pCO2), oxygen partial pressure (pO2), oxygen saturation (SatO2) and bicarbonate (HCO3J, behaviour (head-shaking, sneezing, gasping and gagging) and physiological reflexes (corneal reflex, breathing and sensitivity to pain). Fourteen male lambs of the Ripollesa breed, weighing between 19 and 25 kg were progressively exposed to an atmosphere of 90% CO2 over 66 s. All blood parameters changed between 23 and 43 s after the onset of the immersion. The MLAEP did not decrease significantly until after 48 s exposure to CO2, suggesting an absence of auditory-evoked brain activity. Before that, lambs exhibited head-shaking and sneezing starting at 10.6 (± 0.77) s, and gasping starting at 20.6 (± 1.36) s. After exposure, all animals showed absence of breathing and sensibility to pain, and 36% of them absence of corneal reflex. The pH and pCO2 recovered basal values at 90 and 120 s, respectively, after the end of the exposure. The burst suppression index (BS%) and the A-Line ARX index (AAI) recovered basal levels at 116 and 159 s, respectively. Exposure to CO2 at high concentration induces effective stunning in sheep for a period of 124 s. However, during exposure, the animals exhibited signs of aversion and breathlessness.

Keywords

animal welfareauditory-evoked potentialaversioncarbon dioxidesheepstunning
Type
Research Article
Information
Animal Welfare , Volume 25 , Issue 1 , February 2016 , pp. 73 - 82
Copyright
© 2016 Universities Federation for Animal Welfare

References

Atkinson, S, Velarde, A, Llonch, P and Algers, B 2012 Assessing pig welfare at stunning in Swedish commercial abattoirs using CO2 group-stun methods. Animal Welfare 21: 487–495. http://dx.doi.org/10.7120/09627286.21.4.487CrossRefGoogle Scholar
Cantieni, J 1976 Ein Beitrag zur carbon dioxide-Betäubung von Schlachtschweinen. Schweizer Archiv für Tierheilkunde 119: 255–275. [Title translation: A contribution to the carbon dioxide stun-ning in slaughter pigs]Google Scholar
Capaul, EG 1995 Intercambio de gases a través de la membrana respiratoria. In: García Sacristán, A (ed) Fisiología Veterinaria pp 396–402. McGraw-Hill Interamericana: Madrid, Spain. [Title translation: Gas exchange across respiratory membranes]Google Scholar
Cooper, J, Mason, G and Raj, M 1998 Determination of aversion of farmed mink (Mustela vison) to carbon dioxide. The Veterinary Record 143: 359–361. http://dx.doi.org/10.1136/vr.143.13.359CrossRefGoogle ScholarPubMed
Dalmau, A, Llonch, P, Rodríguez, P, Ruíz-de-la-Torre, JL, Manteca, X and Velarde, A 2010 Stunning pigs with different gas mixtures. Part 1: gas stability. Animal Welfare 19: 315–323Google Scholar
Danneman, PJ, Stein, S and Walshaw, SO 1997 Humane and practical implications of using carbon dioxide mixed with oxygen for anaesthesia or euthanasia of rats. Laboratory Animal Science47: 376-385Google Scholar
EC (European Community) 1993 Council Directive No 93/119/EC. The Protection of Animals at the Time of Slaughter or Killing. EC: Brussels, BelgiumGoogle Scholar
EC (European Community) 2009 Council Regulation No 1099/2009 on the Protection of Animals at the Time of Killing. Official Journal of the European Union L303: 1-30Google Scholar
EFSA 2004 Welfare aspects of animal stunning and killing methods. Scientific report of the Scientific Panel for Animal Health and Welfare on a request from the Commission. Question. Adopted on the 15th of June 2004. Brussels. http://www.efsa.eu.int/science/ahaw/ahaw_opin-ions/495/opinion_ahaw_02_ej45_stunning_report_v2_en1.pdfCrossRefGoogle Scholar
EFSA AHAW Panel (EFSA Panel on Animal Health and Welfare) 2013 Guidance on the assessment criteria for studies evaluating the effectiveness of stunning method s regarding animal protection at the time of killing. EFSA Journal 11(12): 3486Google Scholar
Eisele, JH, Eger, EI and Muallem, M 1967 Narcotic properties of carbon dioxide in the dog. Anesthesiology 28: 856–865. http://dx.doi.org/10.1097/00000542-196709000-00019CrossRefGoogle ScholarPubMed
Forslid, A 1987 Transient neocortical, hippocampal and amyg-daloid EEG silence induced by one minute inhalation of high con-centration CO2 in swine. Acta Physiologica Scandinava 130: 1–10. http://dx.doi.org/10.1111/j.1748-1716.1987.tb08104.xCrossRefGoogle Scholar
Forslid, A 1992 Muscle spasms during pre-slaughter carbon diox-ide anaesthesia in swine. Fleischwirtschaft 72: 167–168Google Scholar
Gregory, NG 1987 The physiology of electrical stunning and slaughter. Humane Slaughter of Animals for Food pp 3-14. UFAW: Wheathampstead, Herts, UKGoogle Scholar
Gregory, NG, Raj, M, Audsley, A and Daly, CC 1990 Effect of CO2 on man. Fleischwirtschaft 70: 1173–1174Google Scholar
Hoenderken, R 1983 Electrical and carbon dioxide stunning of pigs for slaughter. In: Eikelenboom, G (ed) Stunning of Animals for Slaughter pp 59–63. Martinus Nijhoff Publishers: Boston, USAGoogle Scholar
Holst, S 2001 CO2 stunning of pigs for slaughter, practical guide-lines for good animal welfare. 47th International Congress of Meat Science and Technology. 26–31 August 2001, Krakow, PolandGoogle Scholar
Jensen, EW 1999 Monitoring depth of anaesthesia by Auditory Evoked Potentials. Faculty of Health Science, Odense University of Southern Denmark, Odense, DenmarkGoogle Scholar
Jensen, EW, Lindholm, P and Henneberg, SW 1996 Autoregressive modelling with exogenous input of middle-latency auditory-evoked potentials to measure rapid changes in depth of anaesthesia. Methods of Inform Action in Medicine 35: 256–260Google Scholar
Jensen EW, Marianne N, Oteen W and Henneberg 1998 On-line analysis of middle latency auditory evoked potentials (MLAEP) for monitoring depth of anaesthesia in laboratory rats. Medical Engineering & Physical 20: 722–728Google Scholar
Kiley, M 1972 The vocalisations of ungulates, their causation and function. Zeitschrift fur Tierpsychologie 31: 171–222. http://dx.doi.org/10.1111/j.1439-0310.1972.tb01764.xCrossRefGoogle ScholarPubMed
Kirkden, RD, Niel, L and Weary, DM 2005 How aversive is gra-dual fill carbon dioxide euthanasia for rats? CALAS/ACSAL 44th Annual Symposium pp 31. 25-28 June 2005, Vancouver, BC, CanadaGoogle Scholar
Lambooij, E, Gerritzen, MA, Engel, B, Hillebrand, SJW, Lankhaar, J and Pieterse, C 1999 Behavioural responses during exposure of broiler chickens to different gas mixtures. Applied Animal Behaviour Science 62: 255–265. http://dx.doi.org/10.1016/S0168-1591(98)00214-7CrossRefGoogle Scholar
Leach, MC, Bowell, VA, Allan, TF and Morton, DB 2001 Degrees of aversion shown by rats and mice to different concen-trations of inhalational anaesthetics. The Veterinary Record 150:808–815. http://dx.doi.org/10.1136/vr.150.26.808CrossRefGoogle Scholar
Litvan, H, Jensen, EW, Revuelta, M, Henneberg, SW, Paniagua, P, Campos, JM, Martínez, P, Caminal, P and Villar Landeira, JM 2002 Comparison of Auditory Evoked Potentials and the A-line ARX index for monitoring the hypnotic level during sevoflurane and propofol induction. Acta Anaesthetic Scandinavica 46: 245–252. http://dx.doi.org/10.1034/j.1399-6576.2002.t01-1-460304.xCrossRefGoogle ScholarPubMed
Llonch, P, Dalmau, A, Rodríguez, P, Manteca, X and Velarde, A 2012 Aversion to nitrogen and CO2 mixtures for stunning pigs. Animal Welfare 21: 33–39. http://dx.doi.org/10.7120/096272812799129475CrossRefGoogle Scholar
Lomholt, L 1998 Changes in blood gases and acid-base values during high concentration pre-slaughter CO2 stunning of pigs. Doctoral Thesis, The Royal Veterinary and Agricultural University. Copenhagen, DenmarkGoogle Scholar
Manning, HL and Schwartzstein, RM 1995 Pathophysiology of dyspnea. New England Journal of Medicine 333: 1547–1553. http://dx.doi.org/10.1056/NEJM199512073332307CrossRefGoogle ScholarPubMed
Martoft, L, Jensen, EW, Rodríguez, BE, Jorgensen, PF, Forslid, A and Pedersen, HD 2001 Middle-latency auditory evoked poten-tials during induction of thiopentone anaesthesia in pigs. Laboratory Animals 35: 353–363. http://dx.doi.org/10.1258/0023677011911958CrossRefGoogle ScholarPubMed
Martoft, L, Lomholt, L, Kolthoff, C, Rodríguez, BE, Jensen, EW, Jorgensen, PF, Pedersen, HD and Forslid, A 2002 Effects of CO2 anaesthesia on central nervous system activity in swine. Laboratory Animals 36: 115–126. http://dx.doi.org/10.1258/0023677021912398CrossRefGoogle ScholarPubMed
Martoft, L, Stødkilde-Jørgensen, H, Forslid, A, Pedersen, HD and Jørgensen, PF 2003 CO2 induced acute respiratory aci-dosis and brain tissue intracellular pH: a 31 P NMR study in swine Laboratory Animals 37: 241–248. http://dx.doi.org/10.1258/002367703766453092CrossRefGoogle ScholarPubMed
Murillo, D 1995 Control de la respiración. In: García Sacristán, A (ed) Fisiología Veterinaria pp 409–417. McGraw-Hill Interamericana: Madrid, Spain. [Title translation: Respiratory control]Google Scholar
Panella, N, Dalmau, A, Fàbrega, E, Font, I, Furnols, M, Gispert, M, Tibau, J, Soler, J, Velarde, A, Oliver, MA and Gil, M 2008 Effect of supplementation with natural tranquilizers-MgCO3 and L-tryptophan-on the welfare and on the pork quality of NN and nn hal gene pigs, in non-stressful ante-mortem conditions. Livestock Science 115: 107–117. http://dx.doi.org/10.1016/j.livsci.2007.06.014CrossRefGoogle Scholar
Peppel, P and Anton, F 1993 Responses of rat medullary dorsal horn neurons following intranasal noxious chemical stimulation: effects of stimulus, intensity, duration, and interstimulus interval. Journal of neurophysiology 70: 2260–2274CrossRefGoogle ScholarPubMed
Petersen, GV, Carr, DH, Davies, AS and Pickett, BT 1986 The effect of different methods of electrical stunning of lambs on blood pressure and muscular activity. Meat Science 16: 1–15. http://dx.doi.org/10.1016/0309-1740(86)90008-2CrossRefGoogle ScholarPubMed
Raj, ABM 1999 Behaviour of pigs exposed to mixtures of gases and the time required to stun and kill them: welfare implications. Veterinary Record 144: 165–168. http://dx.doi.org/10.1136/vr.144.7.165CrossRefGoogle ScholarPubMed
Raj, ABM and Gregory, NG 1996 Welfare implications of gas stunning of pigs 2. Stress of induction of anaesthesia. Animal Welfare 5: 71–78Google Scholar
Raj, ABM and Gregory, NG 1995 Welfare implications of the gas stunning of pigs 1. Determination of aversion to the initial inhalation of carbon dioxide or argon. Animal Welfare 4: 273–280Google Scholar
Raj, M 1996 Aversive reactions of turkeys to argon, carbon dio-xide, and a mixture of carbon dioxide and argon. Veterinary Record 138: 592–593. http://dx.doi.org/10.1136/vr.138.24.592CrossRefGoogle Scholar
Rodríguez, P, Dalmau, A, Ruiz-de-la-Torre, JL, Manteca, X, Jensen, EW, Rodríguez, B, Litvan, H and Velarde, A 2008 Assessment of unconsciousness during carbon dioxide stunning in pigs. Animal Welfare 17: 341–349Google Scholar
SAS 2001 SAS for Windows Release v8.2. SAS Institute Inc: Cary, North Carolina, USAGoogle Scholar
Thornton, C, Konieczko, KM, Knight, AB, Kaul, B, Jones, JG, Dore, CJ and White, DC 1989 Effect of propofol on the audito-ry evoked response and oesophageal contractility. British Journal Anaesthetic 63: 411–417. http://dx.doi.org/10.1093/bja/63.4.411CrossRefGoogle Scholar
Troeger, K and Woltersdorf, W 1991 Gas anaesthesia of slaughter pigs. Stunning experiments under laboratory conditions with fat pigs of known halothane reaction type: meat quality and animal protection. Fleischwirtschaft 71: 1063–1068Google Scholar
Velarde, A, Cruz, J, Gispert, M, Carrión, D, Ruiz-de-la-Torre, JL, Diestre, A and Manteca, X 2007 Aversion to carbon dioxide stunning in pigs: effect of the carbon dioxide concentration and the halothane genotype. Animal Welfare 16: 513–522Google Scholar
Velarde, A, Gispert, M, Diestre, A and Manteca, X 2003 Effect of electrical stunning on meat and carcass quality in lambs. Meat Science 63: 35–38. http://dx.doi.org/10.1016/S0309-1740(02)00049-9CrossRefGoogle ScholarPubMed
Velarde, A, Gispert, M, Faucitano, L, Alonso, P, Manteca, X and Diestre, A 2001 Effects of the stunning procedure and the halothane genotype on meat quality and incidence of haemor-rhages in pigs. Meat Science 58: 313–319. http://dx.doi.org/10.1016/S0309-1740(01)00035-3CrossRefGoogle ScholarPubMed
Velarde, A, Ruiz-de-la-Torre, JL, Stub, C, Diestre, A and Manteca, X 2000 Factors affecting the effectiveness of head-only electrical stunning in sheep. Veterinary Record 147: 40–43. http://dx.doi.org/10.1136/vr.147.2.40CrossRefGoogle ScholarPubMed
Vergara, H and Gallego, L 2000 Effect electrical stunning on meat quality of lamb. Meat Science 56: 345–349. http://dx.doi.org/10.1016/S0309-1740(00)00061-9CrossRefGoogle ScholarPubMed