1. Weanling male rats were used in three experiments to evaluate the sequence of limiting amino acids for maximum growth with diets based on wheats containing 10, 13 or 17% crude protein (N×5·71).
2. Lysine was the first-limiting, threonine the second-limiting, and valine or methionine the third- or fourth-limiting amino acid for growth rate and feed-conversion ratio of rats offered the diet based on wheat containing 10% crude protein. A further four amino acids (isoleucine, histidine, tryptophan and tyrosine) were limiting for feed-conversion ratio only.
3. For the diet based on wheat containing 13% crude protein the sequence of limiting amino acids was lysine, threonine and methionine.
4. Only lysine was limiting in the diet based on wheat containing 17% crude protein.
5. Plasma-free amino-acid concentrations were found to be a valuable criterion for prediction of the first-limiting amino acid in diets.
6. Improvement of growth and feed-conversion ratio was observed with diets based on wheat containing 17 or 13% crude protein when the limiting amino acids were supplemented to provide the recommended allowance. Further improvement in feed-conversion ratio was obtained when lysine and threonine were provided in excess of the recommended allowances.
7. There was a lysine-threonine interaction affecting growth rate and feed-conversion ratio, and a lysine-me.thionine interaction affecting feed-conversion ratio.