Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-03T01:45:42.315Z Has data issue: false hasContentIssue false

Stimulation of protein absorption in the newborn piglet's intestine through the use of polyvalent cations

Published online by Cambridge University Press:  02 September 2010

M. W. Smith
Affiliation:
ARC Institute of Animal Physiology, Babraham, Cambridge
K. A. Burton
Affiliation:
ARC Institute of Animal Physiology, Babraham, Cambridge
Get access

Summary

The transport of protein across the small intestine of the newborn pig was measured in vitro. The transport of both bovine immune globulin and bovine plasma albumin showed a large variation between piglets. The polycations poly-ornithine, poly-arginine and poly-lysine stimulated both the transport of globulin and albumin. Protamine, histone and arginine were without effect on protein transport. Poly-ornithine became bound to albumin and to the piglet intestinal mucosa. The amount of poly-ornithine needed to stimulate albumin transport was of the same order as that needed to change the electrophoretic mobility of brush border membranes and some 30 times less than that needed to change the charge on the protein. It is concluded that polycations stimulate protein transport by a direct action on the brush border membrane of the pig intestinal mucosa.

Type
Research Article
Copyright
Copyright © British Society of Animal Science 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bangham, A. D., Flemans, R., Heard, D. H. and Seaman, G. v. F. 1958. An apparatus for micro-electrophoresis of small particles. Nature, Lond. 182: 642644.CrossRefGoogle Scholar
Binns, R. M. 1968. The ontogeny of immune response in the pig. Ph.D. Thesis, Univ. Cambridge.Google Scholar
Brambell, F. W. R. 1970. The Transmission of Passive Immunity from Mother to Young. North Holland Publishing Co, Amsterdam.Google Scholar
Brown, P., Smith, M. W. and Witty, R. 1968. Interdependence of albumin and sodium transport in the foetal and new-born pig intestine. J Physiol. 198: 365381.CrossRefGoogle ScholarPubMed
Gell, P. G. H. 1957. The estimation of the individual human serum proteins by an immunological method. J. clin. Path. 10: 6771.CrossRefGoogle ScholarPubMed
Harrison, D. D. and Webster, H. L. 1964. An improved method for the isolation of brush borders from the rat intestine. Biochim. biophys. Acta 93: 662664.CrossRefGoogle ScholarPubMed
Hoerlein, A. B. 1957. The influence of colostrum on antibody response in baby pigs. J. lmmun. 78: 112117.Google ScholarPubMed
Krebs, H. A. and Henseleit, K. 1932. [Investigations concerning the synthesis of urea in animals]. Hoppe-Seyler's Z. physiol. them. 210: 3366.CrossRefGoogle Scholar
Laurell, C.-B. 1966. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analyt. Biochem. 15: 4552.CrossRefGoogle ScholarPubMed
Perry, G. C. and Watson, J. H. 1967a. Sources of variation in the uptake of a marker antibody in piglets. Anim. Prod. 9: 377384.Google Scholar
Perry, G. C. and Watson, J. H. 1967b. Variation in the absorption of a colostrally secreted marker antibody in piglets. Anim. Prod. 9: 385391.Google Scholar
Porteous, J. W. 1968. The isolation of purified brush borders from rat small intestine. FEBS Letters 1: 4649.CrossRefGoogle ScholarPubMed
Ryser, H. J-P. 1965. Histones and basic polyamino acids stimulate the uptake of albumin by tumour cells in culture. Science, N. Y. 150: 501503.CrossRefGoogle ScholarPubMed
Sanders, E. J. and Bell, L. G. E. 1970. Some physiological and morphological effects of polylysine on Amoeba proteus. J. Cell Sci. 7: 739753.CrossRefGoogle Scholar
Smith, M. W. 1971. Ionic dependence of protein transport across the new-born pig intestine. J. Physiol., Lond. 214: 349363.CrossRefGoogle Scholar
Smith, M. W., Witty, R. and Brown, P. 1968. Effect of poly-L-arginine on rate of bovine IgG transport by newborn pig intestine. Nature, Lond. 220: 387388.CrossRefGoogle ScholarPubMed
Veterinary Investigation Service. 1959. A survey of the incidence and causes of mortality in pigs. I. Sow survey. Vet. Rec. 71: 777786.Google Scholar
Veterinary Investigation Service. 1960. A study of the incidence and causes of mortality in pigs. II. Findings of post-mortem examination of pigs. Vet. Rec. 72: 12401247.Google Scholar