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Ruminal fermentation, methanogenesis and nitrogen utilization of sheep receiving tropical grass hay-concentrate diets offered with Sapindus saponaria fruits and Cratylia argentea foliage

Published online by Cambridge University Press:  18 August 2016

H. D. Hess ,
R. A. Beuret ,
M. Lötscher ,
I. K. Hindrichsen ,
A. Machmüller ,
J. E. Carulla ,
C. E. Lascano  and
M. Kreuzer
H. D. Hess*
Affiliation:
Institute of Animal Science, Animal Nutrition, Swiss Federal Institute of Technology (ETH), ETH Centre/LFW, CH-8092 Zurich, Switzerland
R. A. Beuret
Affiliation:
Institute of Animal Science, Animal Nutrition, Swiss Federal Institute of Technology (ETH), ETH Centre/LFW, CH-8092 Zurich, Switzerland
M. Lötscher
Affiliation:
Institute of Animal Science, Animal Nutrition, Swiss Federal Institute of Technology (ETH), ETH Centre/LFW, CH-8092 Zurich, Switzerland
I. K. Hindrichsen
Affiliation:
Institute of Animal Science, Animal Nutrition, Swiss Federal Institute of Technology (ETH), ETH Centre/LFW, CH-8092 Zurich, Switzerland
A. Machmüller
Affiliation:
Institute of Animal Science, Animal Nutrition, Swiss Federal Institute of Technology (ETH), ETH Centre/LFW, CH-8092 Zurich, Switzerland
J. E. Carulla
Affiliation:
Department of Animal Production, National University of Colombia, Bogotá, Colombia
C. E. Lascano
Affiliation:
Tr opical Grass and Legume Project, CIAT, Cali, Colombia
M. Kreuzer
Affiliation:
Institute of Animal Science, Animal Nutrition, Swiss Federal Institute of Technology (ETH), ETH Centre/LFW, CH-8092 Zurich, Switzerland
*
E-mail: [email protected]
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Abstract

The effects of supplementing a tropical, low-quality grass hay (Brachiaria dictyoneura) with legume foliage (Cratylia argentea) or fruits of the multipurpose tree Sapindus saponaria on ruminal fermentation, methane release and nitrogen (N) utilization were evaluated. Six Swiss White Hill lambs were used in a 6 ✕ 6 Latin-square design with a 3 ✕ 2 factorial arrangement of treatments with measurements of energy metabolism being conducted using open-circuit respiratory chambers. Treatments consisted of three basal diets, either grass alone or legume: grass ratios of 1: 2 or 2: 1. These basal diets were supplemented (1: 3) with a control concentrate or with a concentrate containing 250 g/kg dry matter of S. saponaria fruits. The apparent total tract digestibilities of organic matter (OM) and neutral-detergent fibre (NDF) were reduced and the proportionate crude protein (CP) losses through faeces were increased (P 0·01) by supplementation with S. saponaria, and digestibilities of OM and NDF were linearly reduced (P 0·001) with increasing legume proportion. Body energy retention, however, was similar in all diets. Along with CP intake, the proportionate CP losses through faeces decreased (P 0·001) with increasing legume proportion which was associated with improved (P 0·001) body protein retention and reduced (P 0·1) fat retention. Ruminal fluid ammonia concentration was not significantly affected (P > 0·1) by the inclusion of S. saponaria in the concentrate, but increased linearly (P 0·001) as dietary legume proportion was elevated. Supplementation with fruits of S. saponaria increased (P 0·01) total bacteria count, and decreased (P 0·001) total ciliate protozoa count by more than proportionately 0·50. Daily methane release was reduced (P 0·01) by S. saponaria supplementation in all basal diet types. Although being not clearly affected on a daily basis, methane release relative to body protein retention decreased linearly (P 0·05) with increasing legume proportion. The fact that interactions were mostly non-significant (P > 0·05) indicates that supplementation with S. saponaria fruits is a useful means to reduce methane emission from sheep given both tropical grass-based and grass-legume-based diets. Likewise, including legumes in N-limited tropical diets seems to represent an environmentally friendly way to improve animal productivity.

Keywords

ammoniaBrachiarialegumesmethanesaponinssheep
Type
Ruminant nutrition, behaviour and production
Information
Animal Science , Volume 79 , Issue 1 , August 2004 , pp. 177 - 189
Copyright
Copyright © British Society of Animal Science 2004

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References

Abdulrazak, S. A., Muinga, R. W., Thorpe, W. and Ørskov, E. R. 1996. The effects of supplementation with Gliricidia sepium or Leucaena leucocephala forage on intake, digestion and live-weight gains of Bos taurus ✕ Bos indicus steers offered napier grass. Animal Science 63: 381–388.Google Scholar
Abreu, A., Carulla, J. E., Lascano, C. E., Díaz, T. E., Kreuzer, M. and Hess, H. D. 2004. Effects of Sapindus saponaria fruits on ruminal fermentation and duodenal nitrogen flow of sheep fed a tropical grass diet with and without legume. Journal of Animal Science 82: 1392–1400.CrossRefGoogle ScholarPubMed
Agricultural and Food Research Council. 1993. Energy and protein requirements of ruminants. CAB International, Wallingford, UK.Google Scholar
Argel, P. J. and Lascano, C. E. 1998. [Cratylia argentea (Desvaux) O. Kuntze: a new leguminous shrub for acid soils in subhumid tropical zones.] Pasturas Tropicales 20: 37–43.Google Scholar
Association of Official Analytical Chemists. 1990. Official methods of analysis, 15th edition. AOAC Inc., Arlington, VA.Google Scholar
Balcells, J. A., Guada, J. A., Castrillo, C. and Gasa, J. 1991. Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116: 309–317.CrossRefGoogle Scholar
Brouwer, E. 1965. Report of sub-committee on constants and factors. In Energy metabolism (ed. Blaxter, K. L.), pp. 441–443. Academic Press, London.Google Scholar
Chaudhry, A. S., Cowan, R. T., Granzin, B. C. and Klieve, A. V. 2001. The nutritive value of Rhodes grass (Chloris gayana) when treated with CaO, NaOH or a microbial inoculant and offered to dairy heifers as big-bale silage. Animal Science 73: 329–340.Google Scholar
Chen, X. B., Mathieson, J., Hovel, F. D. DeB. and Reeds, P. J. 1990. Measurement of purine derivatives in urine of ruminants using automated methods. Journal of the Science of Food and Agriculture 53: 23–33.CrossRefGoogle Scholar
Demeyer, D. I. 1981. Rumen microbes and digestion of plant cell walls. Agriculture and Environment 6: 295–337.CrossRefGoogle Scholar
Díaz, A., Avendaño, M. and Escobar, A. 1993. Evaluation of Sapindus saponaria as a defaunating agent and its effects on different ruminal digestion parameters. Livestock Research for Rural Development 5: 1–6.Google Scholar
Fässler, O. M. and Lascano, C. E. 1995. The effects of mixtures of sun-dried tropical shrub legumes on intake and nitrogen balance by sheep. Tr opical Grasslands 29: 92–96.Google Scholar
Fievez, V., Piattoni, F., Mbanzamihigo, L. and Demeyer, D. 1999. Reductive acetogenesis in the hindgut and attempts to its induction in the rumen — a review. Journal of Applied Animal Research 16: 1–22.CrossRefGoogle Scholar
France, J. and Siddons, R. C. 1993. Volatile fatty acid production. In Quantitative aspects of ruminant digestion and metabolism (ed. Forbes, J. M. and France, J.), pp. 107–121. CAB International, Oxon.Google Scholar
Goetsch, A. L. and Owens, F. N. 1985.Effect of sarsaponin on digestion and passage rates in cattle fed medium to low concentrate. Journal of Dairy Science 68: 2377–2384.CrossRefGoogle ScholarPubMed
Hess, H. D., Kreuzer, M., Díaz, T. E., Lascano, C. E., Carulla, J. E., Soliva, C. R. and Machmüller, A. 2003a. Saponin rich tropical fruits affect fermentation and methanogenesis in faunated and defaunated rumen fluid. Animal Feed Science and Technology 109: 79–94.Google Scholar
Hess, H. D., Monsalve, L. M., Lascano, C. E., Carulla, J. E., DíazT. E., and Kreuzer, M. 2003b. Supplementation of a tropical grass diet with forage legumes and Sapindus saponaria fruits: effects on in vitro ruminal nitrogen turnover and methanogenesis. Australian Journal of Agricultural Research 54: 703–713.Google Scholar
Hoffmann, L. 1996. [Energy utilisation and energy requirements.] In Bioenergetik und Stoffproduktion landwirtschaftlicher Nutztiere (ed. Bergner, H. and Hoffmann, L.), pp. 189–335. Harwood Academic Publishers, Amsterdam, The Netherlands.Google Scholar
Hoffmann, L. and Klein, M. 1980. [The dependence of urine energy on the carbon and nitrogen content of the urine of cattle, sheep, pigs and rats.] Archives of Animal Nutrition 30: 743–750.Google Scholar
Hostettmann, K. and Marston, A. 1995. Saponins (chemistry and pharmacology of natural products). Cambridge University Press, Cambridge.Google Scholar
Intergovernmental Panel on Climate Change. 2001. Climate change 2001. The scientific basis. Cambridge University Press, Cambridge.Google Scholar
Johnson, K. A. and Johnson, D. E. 1995. Methane emissions from cattle. Journal of Animal Science 73: 2488–2492.Google Scholar
Jouany, J. P., Demeyer, D. I. and Grain, J. 1988. Effect of defaunating the rumen. Animal Feed Science and Technology 21: 229–265.Google Scholar
Klita, P. T., Mathison, G. W., Fenton, T. W. and Hardin, R. T. 1996. Effects of alfalfa root saponins on digestive function in sheep. Journal of Animal Science 74: 1144–1156.Google Scholar
Kreuzer, M. and Kirchgessner, M. 1985. [Effect of type and amount of starch in the diet for sheep on apparent and true N digestibility and N balance.] Archiv für Tierernährung 35: 723–731.CrossRefGoogle Scholar
Külling, D. R., Menzi, H., Sutter, F., Lischer, P. and Kreuzer, M. 2003. Ammonia, nitrous oxide and methane emissions from differently stored dairy manure derived from grass- and hay-based rations. Nutrient Cycling in Agroecosystems 65: 13–22.CrossRefGoogle Scholar
Leng, R. A. 1990. Factors affecting the utilization of ‘poor-quality’ forages by ruminants particularly under tropical conditions. Nutrition Research Reviews 3: 277–303.CrossRefGoogle ScholarPubMed
Lu, C. D. and Jorgensen, N. A. 1987. Alfalfa saponins affect site and extent of nutrient digestion in ruminants. Journal of Nutrition 117: 919–927.CrossRefGoogle ScholarPubMed
McCaughey, W. P., Wittenberg, K. and Corrigan, D. 1999. Impact of pasture type on methane production by lactating beef cows. Canadian Journal of Animal Science 79: 221–226.CrossRefGoogle Scholar
McSweeney, C. S., Makkar, H. P. S. and Reed, J. D. 2003. Modification of rumen fermentation to reduce adverse effects of phytochemicals. In Matching herbivore nutrition to ecosystems biodiversity, (ed. ´tMannetje, L. Ramírez-Avilés, L., Sandoval-Castro, C. and Ku-Vera, J. C.), proceedings of the sixth international symposium on the nutrition of herbivores, pp. 239–268. Universidad Autonomá de Yucatán, Mérida, Mexico.Google Scholar
Mehrez, A. Z., Ørskov, E. R. and McDonald, I. 1977. Rates of rumen fermentation in relation to ammonia concentration. British Journal of Nutrition 38: 437–443.CrossRefGoogle ScholarPubMed
Muinga, R. W., Topps, J. H., Rooke, J. A. and Thorpe, W. 1995. The effect of supplementation with Leucaena leucocephala and maize bran on voluntary food intake, digestibility, live weight and milk yield of Bos indicus ✕ Bos tauru dairy cows and rumen fermentation in steers offered Pennisetum purpureum ad libitum in the semi-humid tropics. Animal Science 60: 13–23.CrossRefGoogle Scholar
Murray, P. J., Gill, E., Balsdon, S. L. and Jarvis, S. C. 2001. A comparison of methane emission from sheep grazing pastures with differing management intensities. Nutrient Cycling in Agroecosystems 60: 93–97.Google Scholar
Norton, B. W. 1994. Tree legumes as dietary supplements for ruminants. In Forage tree legumes in tropical agriculture (ed. Gutteridge, R. C. and Shelton, H. M.), pp. 192–201. CAB International, Oxon.Google Scholar
Pinares-Patiño, C. S., Ulyatt, M. J., Lassey, K. R., Barry, T. N. and Holmes, C. W. 2003. Rumen function and digestion parameters associated with differences between sheep in methane emissions when fed chaffed lucerne hay. Journal of Agricultural Science, Cambridge 140: 205–214.CrossRefGoogle Scholar
Raaflaub, M. and Lascano, C. E. 1995. The effect of wilting and drying on intake rate and acceptability by sheep of the shrub legume Cratylia argentea. Tr opical Grasslands 29: 97–101.Google Scholar
Rosskopf, R., Rainer, H. and Giesecke, D. 1991. [Purine and pyrimidine metabolites for the evaluation of rumen metabolism: HPLC analyses in milk and blood plasma.] Archives of Animal Nutrition 41: 411–426.Google Scholar
Satter, L. D. and Slyter, L. L. 1974. Effect of ammonia concentration on rumen microbial protein production in vitro . British Journal of Nutrition 32: 199–208.CrossRefGoogle ScholarPubMed
Shannon, D. W. F. 1972. A semi-automated method for the determination of the available carbohydrate content of poultry feeds. The Analyst 97: 209–212.CrossRefGoogle Scholar
Simonson, A. J. and Stewart, J. L. 1994. Gliricidia sepium — a multipurpose forage tree legume. In Forage tree legumes in tropical agriculture (ed. Gutteridge, R. C. and Shelton, H. M.), pp. 31–48. CAB International, Oxon.Google Scholar
Sliwinski, B. J., Soliva, C. R., Machmüller, A. and Kreuzer, M. 2002. Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Animal Feed Science and Technology 101: 101–114.CrossRefGoogle Scholar
Soliva, C. R., Hindrichsen, I. K., Meile, L., Kreuzer, M. and Machmüller, A. 2003. Effects of mixtures of lauric and myristic acid on rumen methanogens and methanogenesis in vitro . Letters in Applied Microbiology 37: 35–39.Google Scholar
Stahl, D. A., Amann, R. I., Poulsen, L. K., Raskin, L. and Capman, W. C. 1995. Use of fluorescent probes for determinative microscopy of methanogenic Archaea. In Archaea: methanogens: a laboratory manual (ed. Sowers, K. R. and H. J Schreier), pp. 111–121. Cold Spring Harbor Laboratory Press, New York, USA.Google Scholar
Statistical Analysis Systems Institute. 1996. SAS/STAT user’s guide: statistics, version 6.12. SAS Institute Inc., Cary, NC.Google Scholar
Tangerman, A. and Nagengast, F. M. 1996. A gas chromatographic analysis of fecal short-chain fatty acids, using the direct injection method. Analytical Biochemistry 236: 1–8.Google Scholar
Thornton, R. F. and Minson, D. J. 1973. The relationship between apparent retention time in the rumen, voluntary intake, and apparent digestibility of legume and grass diets in sheep. Australian Journal of Agricultural Research 23: 889–898.Google Scholar
Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583–3597.CrossRefGoogle ScholarPubMed
Wall, M. E., Krider, M. M., Rothman, E. S. and Hedí, C. R. 1952. Steroidal sapogenins. I. Extraction, isolation and identification. Journal of Biological Chemistry 198: 533–543.CrossRefGoogle Scholar
Wang, Y., McAllister, T. A., Newbold, C. J., Rode, L. M., Cheeke, P. R. and Cheng, K. -J. 1998. Effects of Yucca schidigera extract on fermentation and degradation of steroidal saponins in the rumen simulation technique (RUSITEC). Animal Feed Science and Technology 74: 143–153.Google Scholar
Wang, Y., McAllister, T. A., Yanke, L. J., Xu, Z. J., Cheeke, P. R. and Cheng, K.-J. 2000. In vitro effects of steroidal saponins from Yucca schidigera extract on rumen microbial protein synthesis and ruminal fermentation. Journal of the Science of Food and Agriculture 80: 2114–2122.Google Scholar
Williams, A. G. and Coleman, G. S. 1992. The rumen protozoa. Springer, London.CrossRefGoogle Scholar
Wilson, Q. T. and Lascano, C. E. 1997. [Cratylia argentea as supplement in the utilisation of low-quality grass hay in sheep.] Pasturas Tropicales 19: 2–8.Google Scholar