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Rumen degradation of straw 6. Effect of polysaccharidase enzymes on degradation characteristics of ensiled rice straw

Published online by Cambridge University Press:  02 September 2010

Y. Nakashima ,
E. R. Ørskov ,
P. M. Hotten ,
K. Ambo  and
Y Takase
Y. Nakashima
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
P. M. Hotten
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
K. Ambo
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Y Takase
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

The effects of three variables; polysaccharidase inclusion level (0, 5 and 10 g/kg), moisture content (500, 600 and 700 g/kg dry matter (DM), and particle size (20, 5 and 2 mm), on the quality and degradation characteristics of ensiled rice straw were investigated. The polysaccharidase product used was shown to contain a broad spectrum of activities against polysaccharides typical of plant material. The major activities present being: mixed link glucanase, xylanase and both endo- and exo-cellulase. The straw treated with polysaccharidase had a lower final pH (5·21, 4·87, 4·82; P < 0·01), higher concentrations of lactic acid (1·98, 3·90, 3·67 g/kg; P < 0·01), a lower content of neutral-detergent fibre (689, 630, 621 g/kg DM; P < 0·01) and a higher solubility, measured as washing losses from nylon bags (152, 196, 212 g/kg DM; P < 0·01) for the 0, 5 and 10 g/kg polysaccharidase treatments respectively. The samples were subsequently incubated in nylon bags in the rumen of three sheep for 8, 16, 24, 48 and 72 h to estimate degradation rate and potential degradability using the expression p = a + b (1 – e−ct) where p is degradability at time t and a, b and c are constants. While there was no effect of moisture content or physical form of the straw, the degradation rate constant (c) was increased (P < 0·01) by the addition of polysaccharidase. The maximum potential degradability (a + b), however, was not altered by any of the treatments. The values for c were 0·0498, 0·0677 and 0·0817 per h and for (a + b) were 62·4, 62·1 and 62·8 g/100 g DM for the 0, 5 and 10 g/kg polysaccharidase enzyme additions respectively. It is concluded that the use of polysaccharidase enzymes can assist in the preservation of wet straw and can result in improved degradation characteristics.

Type
Research Article
Information
Animal Production , Volume 47 , Issue 3 , December 1988 , pp. 421 - 427
Copyright
Copyright © British Society of Animal Science 1988

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References

REFERENCES

Association of Official Analytical Chemists. 1975. Official Methods of Analysis of the Association of Official Analytical Chemists. 12th ed. Association of Official Analytical Chemists, Washington. DC.Google Scholar
Autrey, K. M., McCaskey, T. A. and Little, J. A. 1975. Cellulose digestibility of fibrous materials treated with Trichoderma viride cellulase. Journal of Dairv Science 58: 67–71.CrossRefGoogle Scholar
Barker, S. B. and Summerson, W. H. 1941. The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry 137: 535–554.CrossRefGoogle Scholar
Dehority, B. A. and Johnson, R. R. 1961. Effect of particle size upon the in vitro cellulose digestibility of forages by rumen bacteria. Journal of Dairy Science 44: 2242–2249.CrossRefGoogle Scholar
Ford, C. W. 1983. Effect of particle size and delignification on the rate of digestion of hemicellulose and cellulose by cellulase in mature pangola grass stems. Australian Journal of Agricultural Research 34: 241–248.CrossRefGoogle Scholar
Galas, E., Pyc, R., Pyc, K. and Siwinska, K. 1981. Universal method for expressing activity of cellulolytic enzymes. European Journal of Applied Microbiological Biotechnology 11: 229–233.CrossRefGoogle Scholar
Guggolz, J., Kohler, G. O., Klopfenstein, T. J. and Saunders, T. M. 1971. Enzymatic evaluation of processes for improving agricultural wastes for ruminant feed. Journal of Animal Science 33: 167–170.CrossRefGoogle Scholar
Henderson, A. R. and McDonald, P. 1977. The effect of cellulase preparations on the chemical changes during the ensilage of grass in laboratory silos. Journal of the Science of Food and Agriculture 28: 486–490.CrossRefGoogle Scholar
Henderson, A. R., McDonald, P. and Anderson, D. 1982. The effect of a cellulase preparation derived from Trichoderma viride on the chemical changes during the ensilage of grass, lucerne and clover. Journal of the Science of Food and Agriculture 33: 16–20.CrossRefGoogle Scholar
Jones, J. K. N. and Tanaka, Y. 1965. In Methods in Carbohydrate Chemistry, Vol. 5 (ed. Whistler, R. L.), pp. 74–134. Academic Press, New York.Google Scholar
Kay, M., Macdearmid, A. and Massie, R. 1970. Intensive beef production. 11. Replacement of cereals with ground straw. Animal Production 12: 419–424.Google Scholar
Leatherwood, J. M., Mochrie, R. D., Stone, E. J. and Thomas, W. E. 1963. Cellulose degradation by enzymes added to ensiled forages. Journal of Dairy Science 46: 124–127.CrossRefGoogle Scholar
Leatherwood, J. M., Mochrie, R. D. and Thomas, W. E. 1959. Chemical changes produced by a celiulolytic preparation added to silages. Journal of Animal Science 18: 1539 (Abstr.).Google Scholar
McCleary, B. V. 1986. A soluble chromogenic substrate for the assay of (1-3)(1-4)-β-D-glucanase. Carbohydrate Polymers 6: 307–318.CrossRefGoogle Scholar
McCullough, M. E. 1964. Influence of cellulase on silage fermentation. Journal of Dairy Science 47: 342 (Abstr.).Google Scholar
McManus, W. R., Manta, L., McFarlane, J. D. and Gray, A. C. 1972. The effects of diet supplements and gamma irradiation on dissimilation of low-quality roughages by ruminants. 1. Studies on the terylene-bag technique and effects of supplementation of base ration. Journal of Agricultural Science, Cambridge 79: 27–40.CrossRefGoogle Scholar
Mehrez, A. Z. and Ørskov, E. R. 1977. A study of the artificial fibre bag technique for determining the digestibility of feeds in the rumen. Journal of Agricultural Science, Cambridge 88: 645–650.CrossRefGoogle Scholar
Minson, D. J. 1963. The effect of pelleting and watering on the feeding value of roughage — a review. Journal of the British Grassland Society 18: 39–44.CrossRefGoogle Scholar
Nakashima, Y. 1979. Studies on enhancement of the nutritive value of rice straw by sodium hydroxide spraying treatment as a dry process method. Journal of the Faculty of Agriculture, lwate University 14: 187–276.Google Scholar
Nakashima, Y., Ørskov, E. R., Ambo, K. and Takase, Y. 1988. Effect of cellulase enzymes on degradation characteristics of ensiled rice straw. Animal Production 46: 507–508 (Abstr.).Google Scholar
Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153: 370–380.CrossRefGoogle Scholar
Olson, M. and Voelker, H. H. 1961. Effectiveness of enzyme and culture additions on the preservation and feeding value of alfalfa silage. Journal of Dairy Science 44: 1204 (Abstr.).Google Scholar
Ørskov, E. R. and McDonald, I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, Cambridge 92: 499–503.CrossRefGoogle Scholar
Ørskov, E. R., Reid, G. W. and Kay, M. 1988. Prediction of intake by cattle from degradation characteristics of roughages. Animal Production 46: 29–34.Google Scholar
Owen, F. G. 1962. Effect of enzymes and bacitracin on silage quality. Journal of Dairy Science 45: 934–936.CrossRefGoogle Scholar
Pickard, D. W., Swan, H. and Lamming, G. E. 1969. Studies on the nutrition of ruminants. 4. The use of ground straw of different particle sizes for cattle from twelve weeks of age. Animal Production 11: 543–550.Google Scholar
Pritchard, G. I., Pigden, W. J. and Minson, D. J. 1962. Effect of gamma radiation on the utilization of wheat straw by rumen microorganisms. Canadian Journal of Animal Science 42: 215–217.CrossRefGoogle Scholar
Raven, A. M., Forbes, T. J. and Irwin, J. Ft. D. 1969. The utilization by beef cattle of concentrate diets containing different levels of milled barley straw and of protein. Journal of Agricultural Science, Cambridge 73: 355–363.CrossRefGoogle Scholar
Shultz, T. A., Ralston, A. T. and Shultz, E. 1974. Effect of various additives on nutritive value of ryegrass straw silage. 1. Laboratory silo and in vitro dry matter digestion observations. Journal of Animal Science 39: 920–925.CrossRefGoogle Scholar
Snedecor, G. W. and Cochran, W. G. 1976. Statistical Methods. 7th ed. Iowa State University Press, Ames, la.Google Scholar
Theander, O. and Aman, P. 1984. In Straw and Other Fibrous By-Products as Feed (ed. SundstØl, F. and Owen, E.), pp. 45–78. Elsevier.Google Scholar
Tomlin, D. C., Johnson, R. R. and Dehority, B. A. 1965. Relationship of lignification to in vitro cellulose digestibility of grasses and legumes. Journal of Animal Science 2A: 161–165.CrossRefGoogle Scholar
Van Soest, P. J. 1963. Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fiber and lignin. Journal of the Association of Official Agricultural Chemists 46: 829–835.Google Scholar
Whitemore, C. T. and Henderson, A. R. 1977. The digestibility of grass silage by sows. Journal of the Science of Food and Agriculture 28: 506–510.CrossRefGoogle Scholar
Wilkins, R. J., Hutchinson, K. J., Wilson, R. F. and Harris, C. E. 1971. The voluntary intake of silage by sheep. I. Interrelationship between silage composition and intake. Journal of Agricultural Science, Cambridge 77: 531–537.CrossRefGoogle Scholar