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Rumen degradation of straw 6. Effect of polysaccharidase enzymes on degradation characteristics of ensiled rice straw

Published online by Cambridge University Press:  02 September 2010

Y. Nakashima ,
E. R. Ørskov ,
P. M. Hotten ,
K. Ambo  and
Y Takase
Y. Nakashima
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
P. M. Hotten
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
K. Ambo
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Y Takase
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

The effects of three variables; polysaccharidase inclusion level (0, 5 and 10 g/kg), moisture content (500, 600 and 700 g/kg dry matter (DM), and particle size (20, 5 and 2 mm), on the quality and degradation characteristics of ensiled rice straw were investigated. The polysaccharidase product used was shown to contain a broad spectrum of activities against polysaccharides typical of plant material. The major activities present being: mixed link glucanase, xylanase and both endo- and exo-cellulase. The straw treated with polysaccharidase had a lower final pH (5·21, 4·87, 4·82; P < 0·01), higher concentrations of lactic acid (1·98, 3·90, 3·67 g/kg; P < 0·01), a lower content of neutral-detergent fibre (689, 630, 621 g/kg DM; P < 0·01) and a higher solubility, measured as washing losses from nylon bags (152, 196, 212 g/kg DM; P < 0·01) for the 0, 5 and 10 g/kg polysaccharidase treatments respectively. The samples were subsequently incubated in nylon bags in the rumen of three sheep for 8, 16, 24, 48 and 72 h to estimate degradation rate and potential degradability using the expression p = a + b (1 – e−ct) where p is degradability at time t and a, b and c are constants. While there was no effect of moisture content or physical form of the straw, the degradation rate constant (c) was increased (P < 0·01) by the addition of polysaccharidase. The maximum potential degradability (a + b), however, was not altered by any of the treatments. The values for c were 0·0498, 0·0677 and 0·0817 per h and for (a + b) were 62·4, 62·1 and 62·8 g/100 g DM for the 0, 5 and 10 g/kg polysaccharidase enzyme additions respectively. It is concluded that the use of polysaccharidase enzymes can assist in the preservation of wet straw and can result in improved degradation characteristics.

Type
Research Article
Information
Animal Production , Volume 47 , Issue 3 , December 1988 , pp. 421 - 427
Copyright
Copyright © British Society of Animal Science 1988

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