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The route of absorbed nitrogen into milk protein

Published online by Cambridge University Press:  09 March 2007

H. Lapierre ,
R. Berthiaume ,
G. Raggio ,
M. C. Thivierge ,
L. Doepel ,
D. Pacheco ,
P. Dubreuil  and
G. E. Lobley
H. Lapierre*
Affiliation:
Agriculture and Agri-Food Canada, Lennoxville, QC, Canada, J1M 1Z3
R. Berthiaume
Affiliation:
Agriculture and Agri-Food Canada, Lennoxville, QC, Canada, J1M 1Z3
G. Raggio
Affiliation:
Université Laval, Québec, QC G1K 7P4, Canada
M. C. Thivierge
Affiliation:
Université Laval, Québec, QC G1K 7P4, Canada
L. Doepel
Affiliation:
Université Laval, Québec, QC G1K 7P4, Canada
D. Pacheco
Affiliation:
Agriculture and Agri-Food Canada, Lennoxville, QC, Canada, J1M 1Z3
P. Dubreuil
Affiliation:
Université de Montréal, St-Hyacinthe, Québec, Canada
G. E. Lobley
Affiliation:
Rowett Research Institute, Aberdeen AB21 9SB, UK
*
†E-mail: [email protected]
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Abstract

A database reviewing the metabolism of nitrogen (N) compounds from absorption to milk has been compiled from 14 published and unpublished studies (33 treatments) that measured the net flux of N compounds across the splanchnic tissues in dairy cows. Apparent N digestibility averaged 0·65, with this then partitioned between 0·34 excreted in urine and 0·31 secreted as milk.

Nitrogen metabolites are absorbed from the lumen of the gut into the portal vein, mainly as free amino acids (AA) and ammonia; these represented 0·58 and 0·57 of digested N, respectively. All of the ammonia absorbed was removed by the liver with, as a result, a net splanchnic flux of zero. Detoxification of ammonia by the liver and catabolism of AA results in production of urea as an end-product. Hepatic ureagenesis is a major cross-road in terms of whole body N exchange, being the equivalent of 0·81 of digested N. Therefore, salvage of a considerable part of this ureagenesis is needed to support milk protein synthesis. This salvage occurs via transfer of urea from the blood circulation into the lumen of the gut. On average, 0·47 of hepatic ureagenesis was returned to the gut via the portal-drained viscera (equivalent to 0·34 of digested N) with 0·56 of this then used for anabolic purposes e.g. as precursor N for microbial protein synthesis. On average, 0·65 of estimated digestible AA was recovered in the portal vein. This loss (0·35) is due to oxidation of certain AA across the gut wall and non-absorption of endogenous secretions. The magnitude of this loss is not uniform among AA and varies between less than 0·05 for histidine to more than 0·90 for some non-essential AA, such as glutamine.

A second database (six studies, 14 treatments) was constructed to further examine the subsequent fate of absorbed essential AA. When all AA are aggregated, the liver removed, on average, 0·45 of portal absorption but this value hides the considerable variation between individual AA. Simplistically, the AA behave as two major groups: one group undergoes very little hepatic removal and includes the branched-chain AA and lysine. For the second group, removal varies between 0·35 and 0·50 of portal absorption, and includes histidine, methionine and phenylalanine. For both groups, however, the efficiency of transfer of absorbed AA into milk protein decreases with increasing supply of protein. This loss of efficiency is linked directly with increased hepatic removal of AA from the second group and, probably, increased catabolism by peripheral tissues, including the mammary gland, of AA from the first group. Therefore, we must stop using fixed factors of conversion of digestible AA to milk in our predictive schemes and acknowledge that metabolism of AA between delivery from the duodenum and conversion to milk protein will vary with nutrient supply. New information evolving from re-analysis of the literature and recent studies will allow better models to be devised for the prediction of nutrient-based responses by the lactating cow. Consideration of biological efficiency, rather than maximal milk yield, will lead to systems that are economically more sensible for the farmer and that have better environmental impacts.

Keywords

amino acidsdairy cowslivermammary glandssmall intestine
Type
Research Article
Information
Animal Science , Volume 80 , Issue 1 , February 2005 , pp. 11 - 22
Copyright
Copyright © British Society of Animal Science 2005

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Footnotes

‡University of Alberta, Edmonton, Alberta, T6G 2P5, Canada
§AgResearch Ltd, Private Bag 11008, Palmerston North, New Zealand

References

Agricultural and Food Research Council. 1993. Energy and protein requirements of ruminants. CAB International, Wallingford.Google Scholar
Bach, A., Huntington, G. B., Calsamiglia, S. and Stern, M. D. 2000. Nitrogen metabolism of early lactation cows fed diets with two different levels of protein and different amino acids. Journal of Dairy Science 83: 2585–2595.Google Scholar
Balcells, J., Parker, D. S. and Seal, C. J. 1992. Purine metabolite concentrations in portal and peripheral blood of steers, sheep and rats. Comparative Biochemistry and Physiology 101B: 633–636.Google Scholar
Bequette, B. J., Hanigan, M. D., Calder, A. G., Reynolds, C. K., Lobley, G. E. and MacRae, J. C. 2000. Amino acid exchange by the mammary gland of lactating goats when histidine limits milk production. Journal of Dairy Science 83: 765–775.Google Scholar
Bequette, B. J., Metcalf, J. A., Wray-Cahen, D., Backwell, F. R. C., Sutton, J. D., Lomax, M. A., MacRae, J. C. and Lobley, G. E. 1996. Leucine and protein metabolism in the lactating dairy cow mammary gland: responses to supplemental dietary crude protein intake. Journal of Dairy Research 63: 209–222.Google Scholar
Bergman, E. N. and Pell, J. M. 1985. Integration of amino acid metabolism in the ruminant. In Herbivore nutrition in the subtropics and tropics(ed. Gilchrist, F. M. C. and Mackie, R. I.), pp. 613–628. The Science Press, Johannesburg.Google Scholar
Berthiaume, R., Dubreuil, P., Stevenson, M., McBride, B. W. and Lapierre, H. 2001. Intestinal disappearance, mesenteric and portal appearance of amino acids in dairy cows fed ruminally protected methionine. Journal of Dairy Science 84: 194–203.CrossRefGoogle ScholarPubMed
Berthiaume, R., Thivierge, M. C., Lobley, G. E., Dubreuil, P., Babkine, M. and Lapierre, H. 2002. Effect of a jugular infusion of essential amino acids on splanchnic metabolism in dairy cows fed a protein deficient diet. Journal of Animal Science 80: /Journal of Dairy Science 85: (suppl. 1) 72–73 (abstr. ).Google Scholar
Berthiaume, R., Thivierge, M. C., Lobley, G. E., Dubreuil, P., Babkine, M. and Lapierre, H. 2003. Effect of a jugular infusion of essential AA on splanchnic metabolism of glucose and hormones in dairy cows fed a protein deficient diet. In Progress in research on energy and protein metabolism(ed. Souffrant, W. B. and Metges, C. C.), European Association for Animal Production publication no. 109, pp. 125–128.Google Scholar
Blouin, J. P., Bernier, J. F., Reynolds, C. K., Lobley, G. E., Dubreuil, P. and Lapierre, H. 2002. Effect of supply of metabolizable protein on splanchnic fluxes of nutrients and hormones in lactating dairy cows. Journal of Dairy Science 85: 2618–2630.Google Scholar
Bruckental, I., Huntington, G. B., Kirk Baer, C. and Erdman, R. A. 1997. The effect of abomasal infusion of casein and recombinant somatotropin hormone injection on nitrogen balance and amino acid fluxes in portal-drained viscera and net hepatic and total splanchnic blood in Holstein steers. Journal of Animal Science 75: 1119–1129.Google Scholar
Casse, E. A., Rulquin, H. and Huntington, G. B. 1994. Effect of mesenteric vein infusion of propionate on splanchnic metabolism in primiparous Holstein cows. Journal of Dairy Science 77: 3296–3303.Google Scholar
Chen, X. B. and Gomes, M. J. 1992. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives – an overview of the technical details. International Feed Resources Unit, Rowett Research Institute, Aberdeen.Google Scholar
Clark, J. H., Klusmeyer, T. H. and Cameron, M. R. 1992. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. Journal of Dairy Science 75: 2304–2323.CrossRefGoogle Scholar
De Lange, C. F., Sauer, W. C., Souffrant, W. B. and Lien, K. A. 1992. 15N-leucine and 15N-isoleucine isotope dilution techniques versus the 15N-isotope dilution technique for determining the recovery of endogenous protein and amino acids in digesta collected from the distal ileum in pigs. Journal of Animal Science 70: 1848–1856.Google Scholar
Delgado-Elorduy, A., Theurer, B., Huber, T., Alio, A., Lozano, O., Sadik, M., Cuneo, P., De Young, H. D., Simas, I. J., Santos, J. E. P., Nussio, L., Nussio, C., Webb Jr, K. and Tagari, H. 2002a. Splanchnic and mammary nitrogen metabolism by dairy cows fed dry-rolled or steam-flaked sorghum grain. Journal of Dairy Science 85: 148–159.Google Scholar
Delgado-Elorduy, A., Theurer, B., Huber, T., Alio, A., Lozano, O., Sadik, M., Cuneo, P., De Young, H. D., Simas, I. J., Santos, J. E. P., Nussio, L., Nussio, C., Webb, K. Jr and Tagari, H. 2002b. Splanchnic and mammary nitrogen metabolism by dairy cows fed steam-rolled or steam-flaked corn. Journal of Dairy Science 85: 160–168.Google Scholar
DeSantiago, S., Torres, N., Suryawan, A., Tovar, A. R. and Hutson, S. M. 1998. Regulation of branched-chain amino acid metabolism in the lactating rat. Journal of Nutrition 128: 1165–1171.Google Scholar
Doepel, L., Hanigan, M. D., Kennelly, J. J., Pacheco, D., López-Campbell, I. F. and Lapierre, H. 2004. Milk protein synthesis as a function of amino acid supply. Journal of Dairy Science 87: 1279–1297.Google Scholar
Gibb, M. J., Ivings, W. E., Dhanoa, M. S. and Sutton, J. D. 1992. Changes in body components of autumn-calving Holstein-Friesian cows over the first 29 weeks of lactation. Animal Production 55: 339–360.Google Scholar
Goodwin, G. W., Gibboney, W., Paxton, R., Harris, R. A. and Lemons, J. A. 1987. Activities of branched-chain amino acid aminotransferase and branched-chain 2-oxo acid dehydrogenase complex in tissues of maternal and fetal sheep. Biochemistry Journal 242: 305–308.Google Scholar
Guinard, J. and Rulquin, H. 1994. Effect of graded levels of duodenal infusions of casein on mammary uptake in lactating cows. 2. Individual amino acids. Journal of Dairy Science 77: 3304–3315.Google Scholar
Huntington, G. B. 1984. Net absorption of glucose and nitrogenous compounds by lactating Holstein cows. Journal of Dairy Science 67: 1919–1927.Google Scholar
Huntington, G. B. 1989. Hepatic urea synthesis and site and rate of urea removal from blood of beef steers fed alfalfa hay or a high concentrate diet. Canadian Journal of Animal Science 69: 215–223.Google Scholar
Huntington, G. B. 1990. Energy metabolism in the digestive tract and liver of cattle: influence of physiological state and nutrition. Reproduction, Nutrition, Development 30: 35–47.CrossRefGoogle ScholarPubMed
Lapierre, H., Bernier, J. F., Dubreuil, P., Reynolds, C. K., Farmer, C., Ouellet, D. R. and Lobley, G. E. 2000. The effect of feed intake level on splanchnic metabolism in growing beef steers. Journal of Animal Science 78: 1084–1099.Google Scholar
Lapierre, H., Blouin, J. P., Bernier, J. F., Reynolds, C. K., Dubreuil, P. and Lobley, G. E. 2002. Effect of diet quality on whole body and splanchnic protein metabolism in lactating dairy cows. Journal of Dairy Science 85: 2618–2630.Google Scholar
Lapierre, H. and Lobley, G. E. 2001. Nitrogen recycling in the ruminant: a review. Journal of Dairy Science 84: (suppl. E) E223–E236.Google Scholar
Lapierre, H., Milne, E., Renaud, J. and Lobley, G. E. 2003. Lysine utilization by the mammary gland. In Progress in research on energy and protein metabolism(ed. Souffrant, W. B. and Metges, C. C.), European Association for Animal Production publication no. 109, pp. 777–780.Google Scholar
Lapierre, H., Ouellet, D. R., Berthiaume, R., Girard, C., Dubreuil, P., Babkine, M. and Lobley, G. E. 2004. Effect of urea supplementation on urea kinetics and splanchnic flux of amino acids in dairy cows. Journal of Animal and Feed Sciences 13: (suppl. 1) 319–322.Google Scholar
LeFloc'h, N., Thibault, J. N. and Sève, B. 1997. Tissue localization of threonine oxidation in pigs. British Journal of Nutrition 77: 593–603.Google Scholar
Lobley, G. E., Bremner, D. M. and Brown, D. S. 2001a. Response in hepatic removal of amino acids by the sheep to short-term infusions of varied amounts of an amino acid mixture into the mesenteric vein. British Journal of Nutrition 85: 689–698.Google Scholar
Lobley, G. E. and Lapierre, H. 2003. Post-absorptive metabolism of amino acids. In Progress in research on energy and protein metabolism(ed. Souffrant, W. B. and Metges, C. C.), European Association for Animal Production publication no. 109, pp. 737–756.Google Scholar
Lobley, G. E., Lapierre, H. and Vázquez-Añón, M. 2001b. HMB metabolism in ruminants. In Proceedings of southwest nutrition and management conference, pp. 15–24.Google Scholar
Lobley, G. E., Shen, X., Le, G., Bremner, D. M., Milne, E., Graham, C. A., Anderson, S. E. and Dennison, N. 2003. Oxidation of essential amino acids by the ovine gastrointestinal tract. British Journal of Nutrition 89: 617–630.Google Scholar
Lobley, G. E., Weijs, P. J., Connell, A., Calder, A. G., Brown, D. S. and Milne, E. 1996. The fate of absorbed and exogenous ammonia as influenced by forage or forage-concentrate diets in growing sheep. British Journal of Nutrition 76: 231–248.Google Scholar
Mabjeesh, S. J., Kyle, C. E., Macrae, J. C. and Bequette, B. J. 2000. Lysine metabolism by the mammary gland of lactating goats at two stages of lactation. Journal of Dairy Science 83: 996–1003.Google Scholar
Martin, A. K. and Blaxter, K. L. 1965. The energy cost of urea synthesis in sheep. In Proceedings of the third symposium on energy metabolism(ed. Blaxter, K. L.), European Association for Animal Production publication no. 11, pp. 83–91. Academic Press, London.Google Scholar
Mepham, T. B. 1982. Amino acid utilization by the mammary gland. Journal of Dairy Science 65: 287–298.Google Scholar
Metges, C. C., Petzke, K. J., El Khoury, A. E., Henneman, L., Grant, I., Bedri, S., Regan, M. M., Fuller, M. F. and Young, V. R. 1999. Incorporation of urea and ammonia nitrogen into ileal and fecal microbial proteins and plasma free amino acids in normal men and ileostomates. American Journal of Clinical Nutrition 70: 1046–1058.Google Scholar
Milano, G. D., Hotston-Moore, A. and Lobley, G. E. 2000. Influence of hepatic ammonia removal on ureagenesis, amino acid utilization and energy metabolism in the ovine liver. British Journal of Nutrition 83: 307–315.Google Scholar
Mukkur, T. K. S., Watson, D. L., Saini, K. S. and Lasdelles, A. K. 1985. Purification and characterization of globlet-cell mucin of high M, from the small intestine of sheep. Biochemistry Journal 229: 419–428.Google Scholar
National Research Council. 2001. Nutrient requirements of dairy cattle, seventh revised edition. National Academy of Sciences, Washington, DC.Google Scholar
Ouellet, D. R., Demers, M., Zuur, G., Lobley, G. E., Seoane, J. R., Nolan, J. V. and Lapierre, H. 2002. Effect of dietary fiber on endogenous nitrogen flows in lactating dairy cows. Journal of Dairy Science 85: 3013–3025.Google Scholar
Parker, D. S., Lomax, M. A., Seal, C. J. and Wilton, J. C. 1995. Metabolic implications of ammonia production in the ruminant. Proceedings of the Nutrition Society 54: 549–563.Google Scholar
Pink, D., Elango, R., Dixon, W. T. and Ball, R. O. 2003. Lysine catabolism in the neonatal piglet during postnatal stages of growth and development. FASEB Journal 17: A702 (abstr. ).Google Scholar
Raggio, G., Lobley, G. E., Pellerin, D., Allard, G., Berthiaume, R., Dubreuil, P., Babkine, M. and Lapierre, H. 2002. Effect of protein intake on synthesis of albumin and plasma total protein in lactating dairy cows. Journal of Animal Science 80: /Journal of Dairy Science 85: (suppl. 1) 243 (abstr. ).Google Scholar
Raggio, G., Pacheco, D., Berthiaume, R., Lobley, G. E., Pellerin, D., Allard, G., Dubreuil, P. and Lapierre, H. 2004. Effect of metabolizable protein on splanchnic flux of amino acids in lactating dairy cows. Journal of Dairy ScienceIn press.Google Scholar
Reynolds, C. K., Aikman, P. C., Lupoli, B., Humphries, D. J. and Beever, D. E. 2003. Splanchnic metabolism of dairy cows during the transition from late gestation through early lactation. Journal of Dairy Science 86: 1201–1217.Google Scholar
Reynolds, C. K., Casper, D. P., Harmon, D. L. and Milton, C. T. 1992a. Effect of CP and ME intake on visceral nutrient metabolism in beef steers. Journal of Animal Science 70: (suppl. 1) 315.Google Scholar
Reynolds, C. K., Huntington, G. B., Tyrrel, H. F. and Reynolds, P. J. 1988. Net portal-drained visceral and hepatic metabolism of glucose, L-lactate, and nitrogenous compounds in lactating Holstein cows. Journal of Dairy Science 71: 1803–1812.Google Scholar
Reynolds, C. K., Lapierre, H., Tyrrell, H. F., Elsasser, T. H., Staples, R. C., Gaudreau, P. and Brazeau, P. 1992b. Effects of growth hormone-releasing factor and feed intake on energy metabolism in growing beef steers: net nutrient metabolism by portal-drained viscera and liver. Journal of Animal Science 70: 752–763.Google Scholar
Symonds, H. W., Mather, D. and Collis, K. A. 1981. The maximum capacity of the liver of adult dairy cows to metabolize ammonia. British Journal of Nutrition 46: 481–486.Google Scholar
Tagari, H., Webb Jr, K., Theurer, B., Huber, T., Cuneo, P., DeYoung, D., Delgado-Elorduy, A., Sadik, M., Alio, A., Lozano, O., Simas, J., Nussio, C., Pu, P., Santos, F. and Santos, J. 2000. Portal drained viscera flux (PDVF) and mammary uptake (MU) of free (FAA) and peptide-bound amino acids (PBAA) in lactating cows fed diets containing steam flaked (SFS) or dry rolled (RDS) sorghum. Journal of Dairy Science 83: /Journal of Animal Science 78: (suppl. 1) 267 (abstr. ).Google Scholar
Tagari, H., Webb Jr, K., Theurer, B., Huber, T., DeYoung, D., Cuneo, P., Santos, J. E. P., Simas, J., Sadik, M., Alio, A., Lozano, O., Delgado-Elorduy, A., Nussio, L., Nussio, C. and Santos, F. 2004. Portal drained viscera flux, hepatic metabolism, and mammary uptake of free and peptide-bound amino acids and milk amino acid output in dairy cows fed diets containing corn grain steam flaked at 360 or steam rolled at 490 g/l. Journal of Dairy Science 87: 413–430.CrossRefGoogle ScholarPubMed
Tamminga, S., Schulze, H., Bruchem van, J., and Huisman, J. 1995. The nutritional significance of endogenous N-losses along the gastro-intestinal tract of farm animals. Archives of Animal Nutrition 48: 9–22.Google Scholar
Thivierge, M. C., Petitclerc, D., Bernier, J. F., Couture, Y. and Lapierre, H. 2002. Variations of mammary metabolism as the udder gradually fills with milk during a 12-h period following milking: leucine kinetics. Journal of Dairy Science 85: 2974–2985.CrossRefGoogle Scholar
Torrallardona, D., Harris, C. I. and Fuller, M. F. 2003. Pigs' gastrointestinal microflora provide them with essential amino acids. Journal of Nutrition 133: 1127–1131.Google Scholar
Visser, H. de, Valk, H., Klop, A., Meulen, J., van der Bakker, J. G. M. and Huntington, G. B. 1997. Nutrient fluxes in splanchnic tissue of dairy cows: influence of grass quality. Journal of Dairy Science 80: 1666–1673.Google Scholar
Wray-Cahen, D., Metcalf, J. A., Backwell, F. R. C., Bequette, B. J., Brown, D. S., Sutton, J. D. and Lobley, G. E. 1997. Hepatic response to increased exogenous supply of plasma amino acids by infusion into the mesenteric vein of Holstein-Friesian cows in late gestation. British Journal of Nutrition 78: 913–930.Google Scholar