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Reproductive toxicological effects of gossypol on male rabbits: semen characteristics and hormonal levels

Published online by Cambridge University Press:  09 March 2007

T. A. Taha ,
W. F. Shaaban ,
A. R. El-Mahdy ,
F. D. El-Nouty  and
M. H. Salem
T. A. Taha*
Affiliation:
Department of Animal Production and Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt
W. F. Shaaban
Affiliation:
Department of Animal Production and Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt
A. R. El-Mahdy
Affiliation:
Department of Food Science and Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt
F. D. El-Nouty
Affiliation:
Department of Animal Production and Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt
M. H. Salem
Affiliation:
Department of Animal Production and Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt
*
†E-mail: [email protected]
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Abstract

The objective of this study was to evaluate the effects of two sublethal doses (4 and 20 mg/kg live weight (LW); every other day) of gossypol on semen and hormonal characteristics of male rabbits. The experiment lasted 16 weeks and included two periods: a treatment period (first 8 weeks) where the animals were given the test materials, and a recovery period (second 8 weeks) where drugs were withdrawn. Results showed that LW and respiration rate (RR) decreased ( P<0·05) among animals that received gossypol, while rectal temperature increased ( P<0·05). Animals in gossypol treatment groups had decreased ( P<0·05) blood plasma levels of tri-iodothyronine (T3) and testosterone. This was accompanied by reductions ( P<0·05) in sperm concentration, total sperm output, percentage incidence of forward motility, total functional sperm fraction and semen initial fructose concentration. Gossypol however increased ( P<0·05) reaction time (i.e. decreased libido), percentages of dead sperm and abnormal sperm (AbSp) and altered acrosomes. Estimated weights of testes and semen ejaculate volume increased ( P<0·05) possibly due to increased water retention. Values of LW, RR, AbSp, and blood plasma T3 and testosterone returned to control levels after withdrawal of gossypol, while the effect of this drug on other parameters continued during the recovery period.

Keywords

gossypolrabbitssemen characterstestosteronetri-iodothyronine
Type
Research Article
Information
Animal Science , Volume 82 , Issue 2 , April 2006 , pp. 259 - 269
Copyright
Copyright © British Society of Animal Science 2006

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References

Adams, R., Geissman, T. A. and Edwards, D. J. 1960. Gossypol, a pigment of cottonseed. Chemical Reviews 60: 555–574.CrossRefGoogle ScholarPubMed
Ahluwalia, B., Hypolite, F. and Anderson, W. 1986. Morphologic and endocrine changes in the reproductive organs in rats implanted with gossypol acetate pellet in the testis. Journal of Andrology 7: 254–263.CrossRefGoogle ScholarPubMed
Arshami, J. and Ruttle, J. L. 1988. Effects of diets containing gossypol on spermatogenic tissues of young bulls. Theriogenology 30: 507–516.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists. 1995. Official methods of analysis, 16th edition. AOAC, Arlington, VA.Google Scholar
Berardi, L. C. and Goldblatt, L. A. 1980. Gossypol. In Toxic constituents of plant food-stuffs (ed. Liener, I. E.), pp. 212–266, Academic Press, New York.Google Scholar
Blom, E. 1983. Pathological conditions in the genital organs and in the semen as grounds for rejection of breeding bulls for import or export to or from Denmark, 1958-1982. Nordisk Veterinaermidicin 35: 105–130.Google ScholarPubMed
Boatner, C. H. 1948. Pigments of cottonseed. In Cottonseed and cottonseed products. Their chemistry and chemical technology (ed. Bailey, A. E.). Wiley (lnterscience), New York.Google Scholar
Boyed, I. L. 1986. Photoperiodic regulation of seasonal testicular regression in the wild European rabbit (Oryctolagus cuniculus). Journal of Reproduction and Fertility 77: 463–470.CrossRefGoogle Scholar
Bryan, J. H. and Akruk, S. R. 1977. A naphthol yellow S and erythrosin B procedure for use in studies of the acrosome reaction of rabbit spermatozoa. Stain Technology 52: 47–51.CrossRefGoogle ScholarPubMed
Coburn, M., Sinsheimer, P., Segal, S., Burgos, M. and Troll, W. 1980. Oxygen-free radical generation by gossypol: a possible mechanism of antifertility action in sea urchin sperm. The Biological Bulletin 159: 468 (abstr.).Google Scholar
Correa, J. R. and Zavos, P. M. 1996. Preparation and recovery of frozen-thawed bovine spermatozoa via various sperm selection techniques employed in assisted reproductive technologies. Theriogenology 46: 1225–1232.CrossRefGoogle ScholarPubMed
Coulson, P. B., Snell, R. L. and Parise, C. 1980. Short term metabolic effects of the anti-fertility agent, gossypol, on various reproductive organs of male mice. International Journal of Andrology 3: 507–518.CrossRefGoogle ScholarPubMed
Del Rio, A. G., Palaoro, L. A., Canessa, O. E. and Blanco, A. M. 2003. Epididymal cytology changes in hypothyroid rats. Archives of Andrology 49: 247–255.CrossRefGoogle ScholarPubMed
De Peyster, A., Hyslop, P. A., Kuhn, C. E. and Sanerhelser, R. D. 1986. Membrane structural/functional perturbations induced by gossypol. Effects on membrane order liposome permeability, and insulin-sensitive hexose transport. Biochemical Pharmacology 35: 3293–3300.CrossRefGoogle ScholarPubMed
De Peyster, A. A. and Srebnik, H. H. 1998. Reproductive endocrine function in gossypol-treated male rats. International Journal of Fertility 33: 362–371.Google Scholar
Donaldson, A., Sufi, S. B. and Jeffcoate, S. L. 1985. Inhibition by gossypol of testosterone production by mouse Leydig cells in vitro. Contraception 31: 165–171.CrossRefGoogle ScholarPubMed
Fisher, R. A. 1949. The design of experiments. Oliver and Boyd, Edinburgh.Google Scholar
Frick, J., Aulitzky, W. and Kalla, N. R. 1988. Clinical microdose study of gossypol: effect on sperm motility and renal function. Contraception 37: 153–162.CrossRefGoogle ScholarPubMed
Jimenez, D. A., Chandler, J. E., Adkinson, R. W., Nipper, W. A., Baham, A. and Saxton, A. M. 1989. Effect of feeding gossypol in cottonseed meal on growth, semen quality, and spermatogenesis of yearling Holstein bulls. Journal of Dairy Science 72: 1866–1875.CrossRefGoogle ScholarPubMed
Kim, I. and Waller, D. P. 1984. Specific inhibition of the testicular mitochondrial respiratory chain in vitro by gossypol. Journal of Andrology 5: 424–430.CrossRefGoogle ScholarPubMed
Kramer, R. Y., Garner, D. L., Ericsson, S. A., Wesen, D. A. and Downing, T. W. 1991. The effect of cottonseed components on testicular development in pubescent ram. Veterinary and Human Toxicology 33: 11–16.Google Scholar
Lin, Y. C., Chitcharoenthum, M. and Rikihisa, Y. 1990. Effect of gossypol on thyroid hormones in young female rats. Contraception 41: 431–440.CrossRefGoogle ScholarPubMed
Lindsey, T. O., Hawkins, G. E. and Guthrie, L. D. 1980. Physiological responses of lactating cows to gossypol from cottonseed meal rations. Journal of Dairy Science 63: 562–573.CrossRefGoogle ScholarPubMed
Liu, G. Z., Lyle, C. and Cao, J. A. 1985. Trail of gossypol as a male contraceptive. In Gossypol, a potential contraceptive for men (ed. Segal, S. J.), pp. 9–16. Plenum Press, New York.Google Scholar
Luginbuhl, J.-M., Poore, M. H. and Conrad, A. P. 2000. Effect of level of whole cottonseed on intake, digestibility, and performance of growing male goats fed hay-based diets. Journal of Animal Science 78: 1677–1683.CrossRefGoogle ScholarPubMed
Mann, T. 1948. Fructose content and fructolysis in semen: practical application in the evaluation of semen quality. Journal of Agricultural Science, Cambridge 38: 323–331.CrossRefGoogle Scholar
Mann, T. 1964. The biochemistry of semen and of the male reproductive tract. John Wiley and Sons, New York.Google Scholar
Matlin, S. A., Zhou, R., Bialy, G., Blye, R. P., Naqvi, R. H. and Lindberg, M. C. 1985. Gossypol: an active male antifertility agent. Contraception 31: 141–149.CrossRefGoogle ScholarPubMed
Moh, P. P., Chang, G. C., Brueggemeier, R. W. and Lin, Y. C. 1993. Effect of gossypol on 5 alpha-reductase and 3 alpha-hydroxysteriod dehydrogenase activities in adult rat testes. Research Communications in Chemical Pathology and Pharmacology 82: 12–26.Google Scholar
Olgiati, K. L., Toscano, D. G., Atkins, W. M. and Toscano, W. A. 1984. Gossypol inhibition of adenylate cyclase. Archives of Biochemistry and Biophysics 231: 411–415.CrossRefGoogle ScholarPubMed
Porat, O. 1990. Effects of gossypol on the motility of mammalian sperm. Molecular Reproduction and Development 25: 400–408.CrossRefGoogle ScholarPubMed
Qian, R. J. and Wang, Z. G. 1984. Gossypol: a potential antifertitity agent for males. Annual Review of Pharmacology and Toxicology 24: 329–360.CrossRefGoogle ScholarPubMed
Randel, R. D., Chase, C. C. and Wyse, S. J. 1992. Effects of gossypol and cottonseed products on reproduction of mammals. Journal of Animal Science 70: 1628–1638.CrossRefGoogle ScholarPubMed
Reyes, J., Wyrick, S. D., Borriero, L. and Benos, D. J. 1986. Membrane actions of male contraceptive gossypol tautomers. Biochimica et Biophysica Acta 863: 101–109.CrossRefGoogle ScholarPubMed
Rikihisa, Y. and Lin, Y. C. 1989. Effect of gossypol on the thyroid in young rats. Journal of Comparative Pathology 100: 411–417.CrossRefGoogle ScholarPubMed
Saksena, S. K. and Salmonsen, R. A. 1982. Antifertility effects of gos-sypol in male hamsters. Fertility and Sterility 37: 686–690.CrossRefGoogle ScholarPubMed
Shandilya, L., Clarkson, T. B., Adams, M. R. and Lewis, J. C. 1982. Effects of gossypol on reproductive and endocrine functions of male cynomolgus monkeys (Macaca fascicularis). Biology of Reproduction 27: 241–252.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute. 1999. SAS user's guide: statistics, version 8 edition. SAS Institute Inc, Cary, NC.Google Scholar
Stephens, D. T., Critchlow, L. M. and Hoskins, D. D. 1983. Mechanism of inhibition by gossypol of glycolysis and motility of monkey spermatozoa in vitro. Journal of Reproduction and Fertility 69: 447–452.CrossRefGoogle ScholarPubMed
Swierstra, E. E. and Foote, R. H. 1965. Duration of spermatogenesis and spermatozoon transport in the rabbit based on cytological changes, DNA synthesis and labeling with tritiated thymidine. American Journal of Anatomy 116: 401–412.CrossRefGoogle ScholarPubMed
Taylor, G. T., Griffin, M. G. and Bardgett, M. 1991. Search for a male contraceptive: the effect of gossypol on sexual motivation and epididymal sperm. Journal of Medicine 22: 29–44.Google ScholarPubMed
Tso, W. W. and Lee, C. S. 1982. Gossypol uncoupling of respiratory chain and oxidative phosphorylation in ejaculated boar spermatozoa. Contraception 25: 649–656.CrossRefGoogle ScholarPubMed
Udoh, P., Patil, D. R. and Deshpande, M. K. 1992. Histopathological and biochemical effects of gossypol acetate on pituitary-gonadal axis of male albino rats. Contraception 45: 493–509.CrossRefGoogle ScholarPubMed
Ueno, H., Sahni, M. K., Segal, S. J. and Koide, S. S. 1988. Interaction of gossypol with sperm macromolecules and enzymes. Contraception 37: 333–341.CrossRefGoogle ScholarPubMed
Velasquez-Pereira, J., Chenoweth, P. J., McDowell, L. R., Risco, C. A., Staples, C. A., Prichard, D., Martin, F. G., Calhoun, M. C., Williams, S. N. and Wilkinson, N. S. 1998. Reproductive effects of feeding gossypol and vitamin E to bulls. Journal of Animal Science 76: 2894–2904.CrossRefGoogle ScholarPubMed
Weinbauer, G. F., Raven, F. and Frick, J. 1982. Antifertility efficacy of gossypol acetic acid in male rats. Andrologia 14: 270–275.CrossRefGoogle ScholarPubMed
Yuan, Y. Y. and Shi, Q. X. 2000. Inhibition of hamster sperm acrosomal enzyme by gossypol is closely associated with the decrease in fertilization capacity. Contraception 62: 203–209.CrossRefGoogle ScholarPubMed