Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-24T01:01:18.540Z Has data issue: false hasContentIssue false
  • English
  • Français

The relationships between leptin and insulin in blood plasma of growing lambs

Published online by Cambridge University Press:  18 August 2016

T. Tokuda ,
D. Kimura  and
T. Fujihara
T. Tokuda
Affiliation:
United Graduate School of Agricultural Science, Tottori University, Tottori-shi 680-8553, Japan
D. Kimura
Affiliation:
Laboratory of Animal Science, Faculty of Life and Environmental Science, Shimane University, Matsue-shi, 690-8504, Japan
T. Fujihara*
Affiliation:
Laboratory of Animal Science, Faculty of Life and Environmental Science, Shimane University, Matsue-shi, 690-8504, Japan
*
Corresponding author: E-mail [email protected]
Get access

Abstract

The present study was designed to examine the pattern of changes in plasma leptin concentration during the early stage of young ruminants. Five crossbred lambs weaned at 45 days were housed individually in pens. The lambs were offered timothy hay, rolled barley and soya-bean meal to meet a 200 g daily gain, and were given free access to water and mineral block. Blood samples were collected just before the morning feeding and at 30-min intervals for 6 h after the morning feeding beginning at 0·5 months after weaning and thereafter monthly for the next 6 months. Plasma leptin level increased dramatically from 0·5 to 3 months after weaning. Thereafter, it dropped to a similar level to that at 2 months after weaning and remained at that level. Moreover, plasma leptin level was not affected by feeding, and no variation throughout the day of sampling was observed. Plasma insulin levels showed similarity to changes in plasma leptin levels. Plasma insulin levels were positively correlated (r = 0·63) with plasma leptin levels. This study shows that plasma leptin level dramatically increases after weaning. Additionally, it suggests that insulin contributes to the increase of leptin with growth in lambs.

Keywords

growthinsulinlambsleptin.
Type
Growth, development and meat science
Information
Animal Science , Volume 73 , Issue 1 , August 2001 , pp. 71 - 76
Copyright
Copyright © British Society of Animal Science 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abe, M. and Iriki, T. 1990. [Regulation of feed intake in calves weaned early.] Proceeding of the Japanese Society of Animal Nutrition and Metabolism 34: 153–169.Google Scholar
Agricultural Research Council. 1984. The nutrient requirements of ruminant livestock, supplement no. 1. Commonwealth Agricultural Bureaux, Farnham Royal, UK.Google Scholar
Arana, A., Soret, B., Mendizabal, J. A., Corroza, M., Eguinoa, P. and Purroy, A. 1998. Changes in adipose tissue accumulation in Rasa Aragonesa breed lambs during growth and fattening. Animal Science 66: 409–413.Google Scholar
Association of Official Analytical Chemists. 1984. Official methods of analysis, 13th edition. Association of Official Analytical Chemists, Washington, DC.Google Scholar
Ballard, F. J., Hansson, R. W. and Kronfeld, D. S. 1969. Gluconeogenesis and lipogenesis in tissue from ruminant and nonruminant animals. Federation Proceedings 28: 218–231.Google Scholar
Barr, V. A., Malide, D., Zarnowski, M. J., Taylor, S. I. and Cushman, S. W. 1997. Insulin stimulates both leptin secretion and production by rat white adipose tissue. Endocrinology 138: 4463–4472.Google Scholar
Blache, D., Tellam, R. L., Chagas, L. M., Blackberry, M. A., Vercoe, P. E. and Martin, G. B. 2000. Level of nutrition affects leptin concentrations in plasma and cerebrospinal fluid in sheep. Journal of Endocrinology 165: 625–637.Google Scholar
Bocquier, F., Bonnet, M., Faulconnier, Y., Guerre-Millo, M., Martin, P. and Chilliard, Y. 1998. Effects of photoperiod and feeding level on perirenal adipose tissue metabolic activity and leptin synthesis in the ovariectomized ewe. Reproduction, Nutrition, Development 38: 489–498.CrossRefGoogle ScholarPubMed
Campfield, L. A., Smith, F. J., Guisez, Y., Devos, R. and Burn, P. 1995. Recombinant mouse ob protein: evidence for a peripheral signal linking adiposity and central neural networks. Science 269: 546–549.Google Scholar
Delavaud, C., Bocquier, F., Chilliard, Y., Keisler, D. H., Gertler, A. and Kann, G. 2000. Plasma leptin determination in ruminants: effect of nutritional status and body fatness on plasma leptin concentration assessed by specific RIA in sheep. Journal of Endocrinology 165: 519–526.Google Scholar
Forbes, J. M. 1995. Growth and fattening. In Voluntary food intake and diet selection in farm animals (ed. Forbes, J. M.), pp. 152–185. CAB International.Google Scholar
Halaas, J. L., Gajiwala, K. S., Maffei, M., Cohen, S. L., Chait, B. T., Rabinowitz, D., Lallone, R. L., Burley, S. K. and Friedman, J. M. 1995. Weight-reducing effects of the plasma-protein encoded by the obese gene. Science 269: 543–546.Google Scholar
Henry, B. A., Goding, J. W., Alexander, A. J., Tilbrook, A. J., Canny, B. J., Du, D., Alexandra, R. A. O., Ashley, M. and Iain, J. C. 1999. Central administration of leptin to ovariectomized ewes inhibits food intake without affecting the secretion of hormones from the pituitary gland: evidence for a dissociation of effects on appetite and neuroendocrine function. Endocrinology 140: 1175–1182.Google Scholar
Hood, R. L. and Thornton, R. F. 1979. The cellularity of ovine adipose tissue. Australian Journal of Agricultural Research 30: 153–161.Google Scholar
Hosoda, K., Masuzaki, H., Ogawa, Y., Miyawaki, T., Hiraoka, J., Hanaoka, I., Yasuno, A., Nomura, T., Fujisawa, Y., Yoshimasa, Y., Nishi, S., Yamori, Y. and Nakao, K. 1996. Development of radioimmunoassay for human leptin. Biochemical and Biophysical Research Communications 221: 234–239.Google Scholar
Johns, J. T. and Bergen, W. G. 1976. Growth in sheep. Preand weaning hormone changes and muscle and liver development. Journal of Animal Science 43: 192–200.CrossRefGoogle Scholar
Mantzoros, C. S., Flier, J. S. and Rogol, A. D. 1997. A longitudinal assessment of hormonal and physical alterations during normal puberty in boys. V. Rising leptin levels may signal the onset of puberty. Journal of Clinical Endocrinology and Metabolism 82: 1066–1070.Google Scholar
Minton, J. E., Bindel, D. J., Drouillard, J. S., Titgemeyer, E. C., Grieger, D. M. and Hill, C. M. 1998. Serum leptin is associated with carcass traits in finishing cattle. Journal of Animal Science 76: (suppl. 1)/ Journal of Dairy Science 81: (suppl. 1) 231.Google Scholar
Rosenbum, M., Nicolson, M., Hirsch, M., Heymsfield, S. B., Gallagher, D., Chu, F. and Leibel, R. L. 1996. Effect of gender, body consumption and menopause on plasma concentrations of leptin. Journal of Clinical Endocrinology and Metabolism 81: 3424–3427.Google Scholar
Roy, J. H. 1980. The calf, fourth edition. Butterworths, London.Google Scholar
Saad, M. F., Riad-Gabriel, M.G, Khan, A., Sharma, A., Michael, R., Jinagouda, S.D., Boyadjian, R. and Steil, G. M. 1998. Diurnal and ultradian rhythmicity of plasma leptin: effects of gender and adiposity. Journal of Clinical Endocrinology and Metabolism 83: 453–459.Google Scholar
Saladin, R. P., De Vos, P., Guerre-Millo, M., Leturque, A., Girard, J., Staels, B. and Auwerx, J. 1995. Transient increase in obese gene expression after food intake or insulin administration. Nature 377: 527–529.Google Scholar
Shimize, H., Shimomura, Y., Nakanishi, Y., Futawatari, T., Ohtani, K., Sato, N. and Mori, M. 1997. Estrogen increases in vivo leptin production in rats and human subjects. Journal of Endocrinology 154: 285–292.Google Scholar
Tokuda, T., Kimura, D. and Fujihara, T. 2000a. Changes in plasma leptin level in growing lambs fed with timothy hay and concentrate. Asian-Australasian Journal of Animal Science 13: (suppl. ) 145.Google Scholar
Tokuda, T., Matsui, T., Ito, J., Torii, S. and Yano, H. 2000b. The changes in body weight and plasma metabolite levels during leptin injection are caused by the reduction of food intake in sheep. Animal Science 70: 343–348.Google Scholar
Tokuda, T., Matsui, T. and Yano, H. 2000c. Effects of light and food on plasma leptin concentrations in ewes. Animal Science 71: 235–242.Google Scholar
Tokuda, T. and Yano, H. 2001. Blood leptin concentrations in Japanese Black cattle. Animal Science 72: 309–313.Google Scholar
Van Harmelen, V., Reynisdottir, S., Eriksson, P., Thorne, A., Hoffstedt, J., Lonnqvist, F. and Arner, P. 1998. Leptin secretion from subcutaneous and visceral adipose tissue in women. Diabetes 47: 913–917.Google Scholar