Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-27T19:05:01.842Z Has data issue: false hasContentIssue false
  • English
  • Français

Polymorphism in exon 2 of the BuLA-DRB3 gene in Indian buffalo (Bubalus bubalis var. indicus) detected by PCR-RFLP

Published online by Cambridge University Press:  18 August 2016

T.V. Aravindakshan ,
A.M. Nainar  and
S.N. Sivaselvam
T.V. Aravindakshan
Affiliation:
Department of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, Tamil Nadu, India
A.M. Nainar
Affiliation:
Department of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, Tamil Nadu, India
S.N. Sivaselvam
Affiliation:
Department of Animal Genetics, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, Tamil Nadu, India
Get access

Abstract

Polymerase chain reaction (PCR) primers specific to exon 2 of the bovine lymphocyte antigen (BoLA)-DRB3 gene were used successfully to amplify the equivalent region in 34 Murrah and 36 Surti buffaloes selected at random. The 304 bp amplified product of the DRB3 gene was separately digested with BstγI, HaeIII and Rsal enzymes. Digestion with BstγI enzyme did not reveal any polymorphism and all animals showed a single restriction pattern, which corresponded exactly to the BstγI pattern ‘b’ previously described for cattle. Digestion with HaeIII enzyme resulted in five patterns, four of which corresponded to the Haelll patterns previously reported in cattle. The new HaeIII pattern was observed in both the breeds of buffaloes studied. The fragment analysis with RsaI revealed 13 different patterns. All of these RsaI patterns corresponded to the RsaI patterns previously described for cattle. The high degree of similarity in the restriction fragment length polymorphism (RFLP) patterns of cattle and buffalo observed in the present study provide evidence for the strong conservation amongst other bovine species, of restriction sites previously reported in cattle.

Keywords

buffaloesgenetic polymorphismpolymerase chain reactionrestriction fragment length polymorphism
Type
Breeding and genetics
Information
Animal Science , Volume 70 , Issue 2 , April 2000 , pp. 221 - 226
Copyright
Copyright © British Society of Animal Science 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allen, P.M., Babbit, B.P. and Unanue, E.R. 1987. T-cell recognition of lysozyme: the biochemical basis of presentation. Immunological Reviews 98:171–187.Google Scholar
Andersson, L., Bohme, J., Rask, L. and Peterson, P. A. 1986. Genomie hybridization of bovine class II major histocompatibility genes. 1. Extensive polymorphism of DQ-oc and DQ-ß genes. Animal Genetics 17: 95–112.CrossRefGoogle Scholar
Bernoco, D., Lewin, H.A., Andersson, L., Arriens, M. A., Byrns, G., Cwik, S., Davies, C.J., Hines, H.C., Leibold, W. and Lie, O. 1991. Joint report of the fourth bovine lymphocyte antigen (BoLA) workshop, East Lansing, Michigan, USA, 25 August, 1990. Animal Genetics 22: 477–496.Google Scholar
Brown, J. H., Jardetzky, T. S., Gorga, J. C., Stern, L. J., Urbem, R. G., Strominger, J. L. and Wiley, D. C. 1993. Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1. Nature 364: 33–39.CrossRefGoogle ScholarPubMed
Davies, C. J., Joosten, I., Andersson, L., Arriens, M. A., Bernoco, D., Bissumbhar, B., Byrns, G., Eijk, M. J. van, Kristensen, B. and Lewin, H. A. 1994. Polymorphism of bovine MHC class II genes. Joint report of the fifth international bovine lymphocyte antigen (BoLA) workshop, Interlaken, Switzerland, 1 August, 1992. European Journal of Immunogenetics 21: 259–289.Google Scholar
Eijk, M. J. T. van, Stewart-Haynes, J. A. and Lewin, H. A. 1992. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Animal Genetics 23: 483–496.Google Scholar
Gelhaus, A., Schnittger, L., Mehlitz, D., Horstmann, R. D. and Mayer, C. G. 1995. Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alíeles. Animal Genetics 26:147–153.Google Scholar
Joosten, I., Hensen, E. J., Sanders, M. F. and Andersson, L. 1990. Bovine MHC class II restriction fragment length polymorphism linked to expressed polymorphism. Immunogenetics 29: 213–216.Google Scholar
Klein, J. 1986. Natural history of the major histocompatibility complex. John Wiley and Son, New York.Google Scholar
Kumar, S., Bhat, P. N., Rasool, T. J. and Bhat, P. P. 1993. Evidence for the possible histocompatibility complex in buffalo (BuLA) using restriction fragment length polymorphism technique. Indian Veterinary Journal 70: 183–184.Google Scholar
Lewin, H. A. and Bernoco, D. 1986. Evidence for BoLA-linked resistance and susceptibility to subclinical progression of bovine leukemia virus infection. Animal Genetics 17:197–207.Google Scholar
Lunden, A., Sigurdardottir, S., Edfors-Lilja, I., Dannel, B., Rendel, J. and Andersson, L. 1990. The relationship between bovine major histocompatibility complex class II polymorphism and disease studied by use of bull breeding values. Animal Genetics 21: 221–232.Google Scholar
Miller, S. A., Dykes, D. D. and Polesky, H. F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16:1215.Google Scholar
Nagarcenkar, R. 1988. Economic traits in milch buffaloes and potential for genetic improvement. In Buffalo production and health. Proceedings of second world buffalo congress, vol. 3, pp. 1–15.Google Scholar
Rao, M. K. and Nagarcenkar, R. 1977. Potentialities of the buffalo. World Review of Animal Production 13: 53–62.Google Scholar
Ryder, L. P., Svejgaard, A. and Dausset, J. 1981. Genetics of HLA disease associations. Annual Review of Genetics 15: 169–187.Google Scholar
Sigurdardottir, S., Borsch, C., Gustafsson, K. and Andersson, L. 1991. Cloning and sequence analysis of 14 DRB alíeles of bovine major histocompatibility complex by using the polymerase chain reaction. Animal Genetics 22: 199–209.Google Scholar
Solbu, H., Spooner, R.L. and Lie, O. 1982. A possible influence of the bovine major histocompatibility complex (BoLA) on mastitis. Proceedings of the second world congress on genetics applied to livestock production, Madrid, vol. VII, pp. 368–371.Google Scholar
Xu, A., Eijk, M. J. T. van, Park, C. and Lewin, H.A. 1993. Polymorphism in BoLA-DRB3 exon 2 correlates with resistance to persistent lymphocytosis caused by bovine leukemia virus. Journal of Immunology 151: 6977–6985.CrossRefGoogle ScholarPubMed
Zanotti, M., Poli, G., Rocchi, M., Bolzani, E., Longeri, M., Russo, S., Lewin, H. A. and Eijk, M. J. T. van. 1996. Association of BoLA class II haplotypes with subclinical progression of bovine leukemia virus infection in Holstein-Friesian cattle. Animal Genetics 27: 337–341.Google Scholar