Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-23T03:38:53.008Z Has data issue: false hasContentIssue false
  • English
  • Français

Metabolic responses to hyperinsulinaemia under glucose-potassium-amino acids clamp in lactating and non-lactating goats

Published online by Cambridge University Press:  02 September 2010

I. Tauveron ,
E. Debras ,
S. Tesseraud ,
Y. Bonnet ,
Ph. Thiéblot ,
C. Champredon  and
J. Grizard
I. Tauveron
Affiliation:
Service d'Endocrinologie CHU 63003 Clermont-Ferrand France
E. Debras
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, INRA Clermont-Ferrand-Theix, 63122 Saint Genès Champanelle, France
S. Tesseraud
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, INRA Clermont-Ferrand-Theix, 63122 Saint Genès Champanelle, France
Y. Bonnet
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, INRA Clermont-Ferrand-Theix, 63122 Saint Genès Champanelle, France
Ph. Thiéblot
Affiliation:
Service d'Endocrinologie CHU 63003 Clermont-Ferrand France
C. Champredon
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, INRA Clermont-Ferrand-Theix, 63122 Saint Genès Champanelle, France
J. Grizard
Affiliation:
Laboratoire d'Etude du Métabolisme Azoté, INRA Clermont-Ferrand-Theix, 63122 Saint Genès Champanelle, France
Get access

Abstract

The present experiment was undertaken to investigate adaptations to insulin action on metabolism during lactation by using plasma concentrations of β hydroxybutyrate (β OH) free fatty acids (FFA) and lactate (L) as indicators. The study included three groups each of four goats. One group was used at 12 to 31 days post partum (early lactation), one group at 98 to 143 days post partum (mid lactation) and one group at 1 year post partum (dry period). For a given physiological state, each goat was examined four times to study the effect of insulin infused for 2·5 h at two rates, medium (0·36 nmol/min) followed by high (1·79 nmol/min) in two protocols: under normal aminoacidaemia in study 1 followed by hyperaminoacidaemia in study 2. Appropriate amino acid infusions were used to blunt insulin-induced hypoaminoacidaemia under eukaliaemic and euglycaemic clamp conditions or to create hyperaminoacidaemia and maintain this state under insulin treatment. In the basal state βOH (P < 0·05), mid lactation) and FFA (P < 0·05 early lactation) were higher during lactation than in the dry period. Plasma L was unmodified. Insulin infusion always resulted in a decrease in βOH levels (P < 0·05). In both studies, the change in βOH concentration as a function of changes in plasma insulin (an index of insulin sensitivity) was greater during early lactation than in the dry period (P < 0·05); this was also the case of mid lactation in study 1. Insulin infusion decreased plasma FFA during early lactation and in the dry period in study 1 (P < 0·05), and there was a trend for insulin sensitivity to be greater during early lactation. In both studies insulin infusion did not affect plasma L in lactating goats whereas plasma L was increased in dry animals (P < 0·05). The results demonstrate that during early lactation, compared with the dry period, there is an increased ability of insulin to lower βOH and FFA concentrations. These effects were not altered by increasing plasma amino acid concentrations during insulin infusion.

Keywords

fatty acidsgoats3-hydroxybutyratehyperinsulinaemialactatelactation
Type
Research Article
Information
Animal Science , Volume 60 , Issue 1 , February 1995 , pp. 99 - 107
Copyright
Copyright © British Society of Animal Science 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Balage, M., Sornet, C. and Grizard, J. 1992. Insulin receptor binding and tyrosine kinase activity in the liver and various skeletal muscles of lactating goats. American Journal of Physiology 262: E561–E568.Google ScholarPubMed
Baracos, V. E., Brun-Bellut, J. and Marie, M. 1991. Tissue protein synthesis in lactating and dry goats. British Journal of Nutrition 66: 451–465.CrossRefGoogle ScholarPubMed
Barnouin, J., El Idibi, N., Chilliard, Y., Chacornac, J. P. and Lefaivre, R. 1986. Micro-dosage automatisé sans déprotéinisation du 3-hydroxybutyrate plasmatique chez les bovins. Annales de Recherches Veterinaires 17: 129.Google Scholar
Bauman, D. E. and Elliott, J. M. 1983. Control of nutrient partitioning in lactating ruminants. In Biochemistry of lactation (ed. Mepham, T. B.), pp. 437–468. Elsevier Science Publishers.Google Scholar
Brockman, R. P. and Laarveld, B. 1985. Effects of insulin on net hepatic metabolism of acetate and β-hydroxybutyrate in sheep (Ovies aries). Comparative Biochemistry and Physiology 81A: 255–257.CrossRefGoogle Scholar
Brockman, R. P. and Laarveld, B. 1986. Hormonal regulation of metabolism in ruminants: a review. Livestock Production Science 14: 313–334.CrossRefGoogle Scholar
Burnol, A. F., Ferré, P., Leturque, A. and Girard, J. 1987. Effect of insulin on in vivo glucose utilization in individual tissues of anesthetized lactating rats. American journal of Physiology 252: E183–E188.Google ScholarPubMed
Burnol, A. F., Leturque, A., Ferré, P., Kande, J. and Girard, J. 1986. Increased insulin sensitivity and responsiveness during lactation in rats. American Journal of Physiology 251: E537–E541.Google ScholarPubMed
Champredon, C., Debras, E., Patureau Mirand, P. and Arnal, M. 1990. Methionine flux and tissue protein synthesis in lactating and dry goats. journal of Nutrition 120: 1006–1015.CrossRefGoogle ScholarPubMed
Chilliard, Y. 1993. Adaptations métaboliques et partage des nutriments chez l'animal en lactation. In Biologic de la lactation (ed Martinet, J. and Houdebine, L. M.), pp. 431–475. INSERM/INRA.Google Scholar
Chilliard, Y., Bauchart, D. and Barnouin, J. 1984. Determination of plasma non esterified fatty acids in herbivores and man: a comparison of values obtained by manual or automatic chromatographic, titrimetric, colorimetric and enzymatic methods. Reproduction, Nutrition, Developpement 24: 469–482.CrossRefGoogle ScholarPubMed
Chilliard, Y., Durand, G., Sauvant, D. and Morand-Fehr, P. 1978. Activitè mètabolique du tissu adipeux de la chévre Hyperinsulinaemia in goats 107 au cours de la gestation et en dèbut de lactation. Comptes Rendus des Sèances de I'Acadèmic des Sciences, Paris 287: 1131–1134.Google Scholar
Chilliard, Y., Sauvant, D., Herview, J., Dorlèans, M. and Morand-Fehr, P. 1977. Lipoprotein lipase activity and composition of omental adipose tissue as related to lipid metabolism of the goat in late pregnancy and early lactation. Annales de Biologic Animate, Biochimie et Biophysique 17: 1021–1033.CrossRefGoogle Scholar
Debras, E., Grizard, J., Aina, E., Tesseraud, S., Champredon, C. and Arnal, M. 1989. Insulin sensitivity and responsiveness during lactation and dry period in goats. American journal of Physiology 256: E295–E302.Google ScholarPubMed
Demignè, C., Yacoub, C., Morand, C. and Rèmèsy, C. 1991. Interactions between propionate and amino acid metabolism in involated sheep hepatocytes. British Journal of Nutrition 65: 301–317.CrossRefGoogle ScholarPubMed
Faulkner, A. and Pollock, H. T. 1990. Metabolic responses to euglycaemic hyperinsulinemia in lactating and nonlactating sheep in vivo, journal of Endrocrinology 124: 59–66.CrossRefGoogle ScholarPubMed
Flakoll, P. J., Kulaylat, M., Frexes-Steed, M., Hourani, H., Brown, L. L., Hill, J. O. and Abumrad, N. N. 1989. Amino acids augment insulin's suppression of whole body proteolysis. American journal of Physiology 257: E839–E847.Google ScholarPubMed
Gagliostro, G., Chilliard, Y. and Davicco, M. J. 1991. Duodenal rapeseed oil infusion in early and midlaction cows. 3. Plasma hormones and mammary apparent uptake of metabolites. journal of Dairy Science 74: 1893–1903.CrossRefGoogle ScholarPubMed
Garlick, P. J. and Grant, I. 1988. Amino acid infusion increases the sensitivity of muscle protein synthesis in vivo to insulin-effect of branched-chain amino acids. Biochemical journal 254: 579–584.CrossRefGoogle ScholarPubMed
Gutmann, I. and Wahlefeld, A. W. 1974. L-(+)-lactate determination with lactate dehydrogenase and NAD. In Methods in enzymatic analysis, vol. 4 (ed. Bergmeyer, H. U.), pp. 1464–1468. Academic Press, London.Google Scholar
Kazumi, T., Vranic, M. and Steiner, G. 1986. Triglyceride kinetics: effects of dietary glucose, sucrose or fructose alone or with hyperinsulinemia. American journal of Physiology 250: E325–E330.Google ScholarPubMed
Metcalf, J. A., Sutton, J. D., Cockburn, J. E., Napper, D. J. and Beever, D. E. 1991. The influence of insulin and amino acid supply on amino acid uptake by the lactating bovine mammary gland. journal of Dairy Science 74: 3412–3420.CrossRefGoogle ScholarPubMed
Metcalf, J. A. and Weekes, T. E. C. 1990. Effect of plane of nutrition on insulin sensitivity during lactation in the ewe. journal of Dairy Research 57: 465–478.CrossRefGoogle ScholarPubMed
Pisters, P. W. T., Restifo, N. P., Cersosimo, E. and Brennan, M. F. 1991. The effects of euglycemic hyperinsulinemia and amino acid infusion on regional and whole body glucose disposal in man. Metabolism 40: 59–65.CrossRefGoogle ScholarPubMed
Rèmèsy, C. and Demignè, C. 1974. Determination of volatile fatty acids in plasma after ethanolic extraction. Biochemical journal 141: 81–91.CrossRefGoogle ScholarPubMed
Tesseraud, S., Grizard, J., Debras, E., Papet, I., Bonnet, Y., Bayle, G. and Champredon, C. 1993. Leucine metabolism in lactating and dry goats: effect of insulin and substrate availability. American Journal of Physiology 265: E402–E413.Google ScholarPubMed
Tesseraud, S., Grizard, J., Makarski, B., Debras, E., Bayle, G. and Champredon, C. 1992. Effect of insulin in conjunction with glucose, amino acids and potassium on net metabolism of glucose and amino acids in the goat mammary gland. Journal of Dairy Research 59: 135–149.CrossRefGoogle ScholarPubMed
Trevisan, R., Nosadini, R., Avogaro, A., Lippe, G., Duner, E., Fioretto, P., Deana, R., Tessari, P., Tiengo, A., Velussi, M., Cemigoi, A., Del Prato, S. and Crepaldi, G. 1986. Type I diabetes is characterized by insulin resistance not only with regard to glucose, but also to lipid and aminoacid metabolism. journal of Clinical Endocrinology and Metabolism 62: 1155–1162.CrossRefGoogle ScholarPubMed
Vernon, R. G. 1989. Endocrine control of metabolic adaptation during lactation. Proceedings of the Nutrition Society 48: 23–32.CrossRefGoogle ScholarPubMed
Vincent, R. and Lindsay, D. B. 1985. Effect of pregnancy and lactation on muscle protein metabolism in sheep. Proceedings of the Nutrition Society 44: 77A.Google Scholar
Weekes, T. E. C. 1992. Influence of experimental hyperthyroidism on insulin action in growing sheep. Metabolism 41: 246–252.CrossRefGoogle ScholarPubMed
Weekes, T. E. C., Sasaki, Y. and Tsuda, T. 1983. Enhanced responsiveness to insulin in sheep exposed to cold. American journal of Physiology 244: E335–E345.Google ScholarPubMed