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Influence of quality of dietary protein supplement and anabolic steroids on muscular and skeletal growth of foals

Published online by Cambridge University Press:  02 September 2010

M. T. Saastamoinen
Affiliation:
Agricultural Research Centre, Equine Research Station, SF-32100 Ypäjä, Finland
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Abstract

Twenty-two weanling foals, about 7 months (215 days) old, were allotted to two groups. One group received high-quality protein (HP; milk powder) and the other received poor-quality protein (PP; barley protein). In addition, six foals in each group were injected intramuscularly (1 mg/kg body weight) with an anabolic steroid (Nandrolon lauras, Laurabolin vet®) at intervals of 3 weeks from weaning to 12 months (362 days) of age. Skeletal and muscular measurements from foals were also made at intervals of 3 weeks. Closure of the right distal growth plate was assessed radiographically in 13 colts in their 2nd and 3rd years of life. Blood samples were taken to investigate the blood chemistry. The HP-group foals gained weight faster from weaning to 12 months of age and were heavier at the age of 12 months than the PP-group foals. The growth of longissimus dorsi muscle and cannon bone circumference were also significantly greater in the HP-group foals. The high-quality dietary protein supplement also increased serum amino acid concentrations and haemoglobin and packed cell volume values. The foals treated with the anabolic steroid increased in body length more slowly from weaning to 12 months of age than the untreated foals. Growth plates closed about 1·5 months later in the horses treated with anabolic steroid than in the untreated horses. Weanling foals seem to require a protein supplement rich in lysine for their optimal growth. More than 31 g/day (0·46 g/MJ metabolizable energy) lysine can be recommended up to 10 months of age.

Type
Research Article
Copyright
Copyright © British Society of Animal Science 1993

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References

Beroza, G. A. 1981. Anabolic steroids in the horse, journal of the American Veterinary Medical Association 179: 278280.Google ScholarPubMed
Burke, P. R., Potter, G. D., McMullan, W. C., Kreider, J. L., Dutson, T. R. and Herring, D. S. 1981. Physiological effects of an anabolic steroid in the growing horse. Proceedings of the seventh equine nutrition symposium, pp. 161164.Google Scholar
Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology. 1974. Recommended methods for the determination of four enzymes in blood. Scandinavian journal of Clinical Laboratory Investigation 33: 291306.CrossRefGoogle Scholar
Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology. 1981. Experiences with the Scandinavian methods for determinations of enzymes in blood. Scandinavian Journal of Clinical Laboratory Investigation 41: 107116.CrossRefGoogle Scholar
Cymbaluk, N. F. 1990. Using canola meal in growing draft horse diets. Equine Practice 12: 1319.Google Scholar
Denny, J. E. F. M. 1985. Equine blood serum calcium and phosphorus concentrations in progressive nutritional hyperparathyroidism. journal of the South African Veterinary Association 56: 123125.Google ScholarPubMed
Dietz, O., Nagel, E. and Schwede, H. 1988. Zur problematik der epiphysenreifung beim pferd. Praktische Tierarzt 69: 2127.Google Scholar
Einarsson, S. 1985. Selective determination of secondary amino acids using precolumn derivatization with 9-fluorenylmethylchloroformate and reversed-phase high performance liquid chromatography. Journal of Chromatography 348: 213220.CrossRefGoogle Scholar
Einarsson, S., Josefsson, B. and Lagerqvist, S. 1983. Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography. journal of Chromatography 282: 609618.CrossRefGoogle Scholar
Fawcett, J. K. and Scott, J. E. 1960. A rapid and precise method for the determination of urea, journal of Clinical Pathology 13: 156159.CrossRefGoogle ScholarPubMed
Fonnesbeck, P. V. and Symons, L. D. 1969. Effect of diet on concentration of protein, urea nitrogen, sugar and cholesterol of blood plasma of horses. Journal of Animal Science 28: 216219.CrossRefGoogle ScholarPubMed
Frape, D. 1986. Equine nutrition and feeding. Longman, Essex.Google Scholar
Fretz, P. B., Cymbaluk, N. F. and Pharr, J. W. 1984. Quantitative analysis of long-bone growth in the horse. American Journal of Veterinary Research 45: 16021609.Google ScholarPubMed
Glade, M. J. 1991. Dietary yeast culture supplementation of mares during late gestation and early lactation — effects on mare and foal plasma metabolite, amino acid and endocrine profiles. Equine Veterinary Science 11: 167175.CrossRefGoogle Scholar
Glade, M. J. and Sist, M. D. 1990. Supplemental yeast culture alters the plasma amino acid profiles of nursling and weanling foal. Equine Veterinary Science 10: 369379.CrossRefGoogle Scholar
Goodbee, R. G. and Slade, L. M. 1981. The effect of urea or soybean meal on the growth and protein status of young horses. Journal of Animal Science 53: 670676.CrossRefGoogle Scholar
Herring, D. S. 1981. Radiographic evaluation of the distal radial physis of horses treated with anabolic steroids. Proceedings of the symposium on anabolic steroids in equine medicine, pp. 1820.Google Scholar
Hintz, H. F., Schryver, H. F. and Lowe, J. E. 1971. Comparison of a blend of milk products and linseed meal as protein supplements for young growing horses. Journal of Animal Science 33: 12741277.CrossRefGoogle ScholarPubMed
Keane, M. G. and Drennan, M. J. 1990. Comparison of growth and carcass composition of heifers in three production systems and steers and effects of implantation with anabolic agents. Irish Journal of Agricultural Research 29: 113.Google Scholar
Keenan, D. M., Bruce, I. J. and Allardyce, C. J. 1987. The effect of breed, date of birth and anabolic steroids on the body weight of foals. Australian Veterinary Journal 64: 32.CrossRefGoogle ScholarPubMed
Koterba, A. ML, Drummond, W. H. and Kosch, P. C. 1990. Equine clinical neonatology. Lea and Febiger, Philadelphia.Google Scholar
Lepage, O. M., Descoteaux, L., Marcoux, M. and Tremblay, A. 1991. Circadian rhythms of osteocalcin in equine serum. Correlation with alkaline phosphatase, calcium, phosphate and total protein levels. Canadian Journal of Veterinary Research 55: 510.Google ScholarPubMed
McDonald, P., Edwards, R. A. and Greenhalgh, J. F. D. 1988. Animal nutrition. 4th ed. Longman, New York.Google Scholar
Mäenpää, P. H., Koskinen, T. and Koskinen, E. 1988a. Serum profiles of vitamins A, E and D in mares and foals during different seasons. Journal of Animal Science 66: 14181423.CrossRefGoogle Scholar
Mäenpää, P. H., Pirskanen, A. and Koskinen, E. 1988b. Biochemical indicators of bone formation in foals after transfer from pasture to stables for the winter months. American Journal of Veterinary Research 49: 19901992.Google ScholarPubMed
Maher, J. M., Squires, E. L., Voss, J. L. and Shideler, R. K. 1983. Effect of anabolic steroid on reproductive function of young mares. Journal of the American Veterinary Medical Association 183: 519524.Google Scholar
Martin-Rosset, W. ed. 1990. Valimentation des chevaux. Techniques et pratiques. Institut National de la Recherche Agronomique, Paris.Google Scholar
Maynard, L. A., Loosli, J. K., Hintz, H. F. and Warner, R. G. 1979. Animal nutrition. 7th ed. Tata McGraw Hill Publishing Company, New Delhi.Google Scholar
Meyer, H. 1986. Pferdefiitterung. Paul Parey, Berlin.Google Scholar
Ministry of Agriculture, Fisheries and Food, Department of Agriculture and Fisheries for Scotland and Department of Agriculture for Northern Ireland. 1975. Energy allowances and feeding systems for ruminants. Technical bulletin 33. Her Majesty's Stationery Office, London.Google Scholar
Näsi, M. 1988. Evaluating barley feed fractions from integrated ethanol-starch production in diets of ruminants. Journal of Agricultural Science in Finland 60: 701709.Google Scholar
National Research Council. 1989. Nutrient requirements of horses. 5th ed. National Academic Press, Washington DC.Google Scholar
O'Connor, J. J., Stillions, M. C., Reynolds, W. A., Linkenheimer, W. H. and Maplesden, D. C. 1973. Evaluation of boldenone undecylenate as an anabolic agent in horses. Canadian Veterinary Journal 14: 154159.Google ScholarPubMed
Ott, E. A., Asquith, R. L., Feaster, J. P. and Martin, F. G. 1979. Influence of protein level and quality on the growth and development of yearling foals. Journal of Animal Science 49: 620628.CrossRefGoogle Scholar
Ott, E. A., Asquith, R. L. and Feaster, J. P. 1981. Lysine supplementation of diets for yearling horses. Journal of Animal Science 53: 14961503.CrossRefGoogle ScholarPubMed
Reitnour, C. M. and Salsbury, R. L. 1976. Utilization of proteins by the equine species. American Journal of Veterinary Research 37: 10651067.Google ScholarPubMed
Rogers, P. A., Fahey, G. C. and Albert, W. W. 1984. Blood metabolite profiles of broodmares and foals. Equine Veterinary Journal 16: 192196.CrossRefGoogle ScholarPubMed
Saastamoinen, M. 1990. Factors affecting growth and development of foals and young horses. Ada Agriculturae Scandinavica 40: 387396.CrossRefGoogle Scholar
Salo, M.-L., Tuori, M. and Kiiskinen, T. 1990. [Peed tables and feeding recommendations.] University of Helsinki, Helsinki.Google Scholar
Sarkar, R. B. C. and Chauhan, U. P. S. 1967. A new method for determining micro quantities of calcium in biological materials. Analytical Biochemistry 20: 155166.CrossRefGoogle ScholarPubMed
Schryver, H. F., Meakam, D. W., Lowe, J. E., Williams, J., Soderholm, L. V. and Hintz, H. F. 1987. Growth and calcium metabolism in horses fed verying levels of protein. Equine Veterinary Journal 19: 280287.CrossRefGoogle Scholar
Sisson, S. and Grossman, J. D. 1975. The anatomy of the domestic animal. 5th ed. W. B. Saunders Co, Philadelphia.Google Scholar
Skjaerlund, D. M., Mulvaney, D. R., Mars, R. H., Schroeder, A. L., Bergen, W. G. and Merkel, R. A. 1988. Measurement of protein turnover in skeletal muscle strips. Journal of Animal Science 66: 687698.CrossRefGoogle ScholarPubMed
Snow, D. H., Munro, C. D. and Nimmo, M. 1977. Anabolic steroid in equine practice. Proceedings of the twenty-third annual convention of the American association of equine practitioners, pp. 411418.Google Scholar
Snow, D. H., Munro, C. D. and Nimmo, M. A. 1982a. Effects of nandrolone phenylpropionate in the horse. 1. Resting animal. Equine Veterinary Journal 14: 219223CrossRefGoogle ScholarPubMed
Snow, D. H., Munro, C. D. and Nimmo, M. A. 1982b. Effects of nandrolone phenylpropionate in the horse. 2. General effects in animals undergoing training. Equine Veterinary Journal 14: 224228.CrossRefGoogle ScholarPubMed
Stanley, S., Tobin, T. and Wood, T. 1989. The effects of steroids. Pacemaker Update International, June 1989.Google Scholar
Trinder, P. 1969. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry 6: 2427.CrossRefGoogle Scholar
Vanderwal, P. 1976. Anabolic agents in animal production. Proceedings of FAO/WHO symposium, pp. 6078.Google Scholar
Weichselbaum, T. E. 1946. An accurate and rapid method for the determination of proteins in small amounts of blood serum and plasma. American Journal of Clinical Pathology 16: 4049.CrossRefGoogle Scholar
Wirth, B. L., Potter, G. D. and Broderick, G. A. 1976. Cottonseed meal and lysine for weanling foals. Journal of Animal Science 43: 261 (abstr.).Google Scholar
Yoakam, S. C., Kirkham, W. W. and Beeson, W. M. 1978. Effect of protein level on growth in young ponies. Journal of Animal Science 46: 983991.CrossRefGoogle ScholarPubMed
Zilversmit, D. B. and Davis, A. K. 1950. Microdetermination of plasma phospholipidus by trichloroacetic acid precipitation. Journal of Laboratory Clinical Medicine 35: 155160.Google ScholarPubMed