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In vitro produced embryos as a means of achieving pregnancy and improving productivity in beef cows

Published online by Cambridge University Press:  02 September 2010

K. D. Sinclair ,
P. J. Broadbent  and
D. F. Dolman
K. D. Sinclair
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen AB9 1UD
P. J. Broadbent
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen AB9 1UD
D. F. Dolman
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen AB9 1UD
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Abstract

The possibilities of using embryo transfer (ET) with in-vitro produced embryos as an alternative to artificial insemination (Al) for achieving pregnancy in commercial cattle, and improving the number and quality of calves produced were assessed using 157 multiparous, beefX. dairy suckling cows (mainly Hereford × Friesian) in three autumn and three spring-calving herds in Scotland. Within each herd, cows were allocated at random to (i) Al; or ET with in-vitro produced embryos, either (ii) singly (ET(s)), or (Hi) in pairs (ET(t)) to establish pregnancy. In-vitro produced embryos were derived from oocytes recovered from the ovaries of heifers with high-quality carcasses post slaughter. Semen from a single Simmental sire was used for in-vitro embryo -production and for Al across all herds. Following oestrous synchronization, Al cows were inseminated at 48 and 72 h after removal of the synchrony device. ET cows that had a palpatable corpus luteum received either one or two in-vitro produced embryos placed non-surgically in the ipsilateral uterine horn on day 7 after oestrous (oestrus = day 0). Pregnancy rate, calving rate and calving difficulty were monitored for all cows. Calf performance was monitored on the farms of origin from birth to purchase and thereafter at one finishing unit until slaughter. Animals were slaughtered at a prescribed level of body fatness and saleable meat yields determined.

Cows that received one in-vitro produced embryo gave birth to fewer calves than cows that were artificially inseminated or received two embryos (P < 0·01). Gestation length was longer for cows that gave birth to single ET calves (288 days; P < 0·01) than cows that gave birth to Al calves (285 days) or twin ET calves (284 days). Single ET calves were heaviest at birth (P < 0·01) and higher levels of assistance at calving were required for ET calves whether born as singles or twins. ET calves of both birth types grew more quickly (<1·00 kg/day; P<0·05) and produced larger carcasses (297 kg; P < 0·01) with higher yields of saleable meat (2207 kg; P < 0·01) than Al calves (0·92 kg/day; 281·7 kg; 206·3 kg respectively).

Keywords

beef cowscarcass compositionembryo transferin vitro culturetwinning
Type
Research Article
Information
Animal Science , Volume 60 , Issue 1 , February 1995 , pp. 55 - 64
Copyright
Copyright © British Society of Animal Science 1995

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References

Agricultural Research Council. 1980. The nutrient requirements of ruminant livestock. Commonwealth Agricultural Bureaux, Slough.Google Scholar
Anderson, G. B., Cupps, P. T., Drost, M., Horton, M. B. and Wright, R. W. 1978. Induction of twinning in heifers by bilateral embryo transfer. Journal of Animal Science 46: 449–452.CrossRefGoogle Scholar
Anonymous. 1993. British Simmental national sire and dam summary. Meat and Livestock Commission and the British Simmental Cattle Society Ltd, Kenilworth.Google Scholar
Benton, T. A. 1986. Gestation length — how important is it? The British Friesian Journal 68: (3), 246–247.Google Scholar
Broadbent, P. J. and Dolman, D. F. 1989. Twinning cattle by embryo transfer. In New techniques in cattle production (ed. Phillips, C. J. C.), pp. 238–239. Butterworths, London.Google Scholar
Broadbent, P. J., Mclntosh, J. A. R. and Spence, A. 1970. The evaluation of a device for feeding group-housed animals individually. Animal Production 12: 245–252.Google Scholar
Cran, D. G., Johnson, L. A., Miller, N. G. A., Cochrane, D. and Polge, C. 1993. Production of bovine calves following separation of X-chromosome and Y-chromosome bearing sperm and in vitro fertilization. Veterinary Record 132: 40–41.CrossRefGoogle Scholar
Diskin, M. G., McDonagh, T. and Sreenan, J. M. 1987. The experimental induction of twin calvings in beef cows by embryo transfer. Theriogenology 27: 224 (abstr.).CrossRefGoogle Scholar
Diskin, M. G., McEvoy, T. G. and Sreenan, J. M. 1990a. Calving performance and calf survival in a twin-calving beef herd. Animal Production 50: 545 (abstr.).Google Scholar
Diskin, M. G., McEvoy, T. G. and Sreenan, J. M. 1990b. A comparison of the growth rate of single and twin-born beef calves. Animal Production 50: 546 (abstr.).Google Scholar
Eddy, R. G., Davies, O. and David, C. 1991. An economic assessment of twin births in British dairy herds. Veterinary Record 129: 526–529.Google ScholarPubMed
Gordon, I. and Lu, K. H. 1990. Production of embryos in vitro and its impact on livestock production. Theriogenology 33: 77–87.CrossRefGoogle Scholar
Gregory, K. E., Echternkamp, S. E., Dickerson, G. E., Cundiff, L. V., Koch, R. M. and Van Vleck, L. D. 1990. Twinning in cattle. III. Effects of twinning on dystocia, reproductive traits, calf survival, calf growth and cow productivity. Journal of Animal Science 68: 3133–3144.CrossRefGoogle ScholarPubMed
Johnson, L. A., Cran, D. G. and Polge, C. 1994. Recent advances in sex preselection of cattle: flow cytometric sorting of X-chromosome and Y-chromosome bearing sperm based on DNA to produce progeny. Theriogenology 41: 51–56.CrossRefGoogle Scholar
Kempster, A. J., Cook, G. L. and Southgate, J. R. 1982. A comparison of different breeds and crosses from the suckler herd. 2. Carcass characteristics. Animal Production 35: 99–111.Google Scholar
King, K. K., Seidel, G. E. and Elsden, R. P. 1985. Bovine embryo transfer pregnancies. II. Lengths of gestation. Journal of Animal Science 61: 758–762.CrossRefGoogle ScholarPubMed
Lowman, B. G., Scott, N. A. and Somerville, S. H. 1976. Condition scoring of cattle. Revised ed. Bulletin, East of Scotland College of Agriculture, no. 6.Google Scholar
Lu, K. H., Gordon, I., Chen, H. B., Gallagher, M. and McGovern, H. 1988. Birth of twins after transfer of embryos produced by in vitro techniques. Veterinary Record 122: 539–540.CrossRefGoogle ScholarPubMed
Lu, K. H., Gordon, I., Gallagher, M. and McGovern, H. 1987. Pregnancy established in cattle by transfer of embryos derived from in vitro fertilization of oocytes matured in vitro. Veterinary Record 121: 259–260.CrossRefGoogle ScholarPubMed
McCutcheon, G. A., Caffrey, P. J., Kelleher, D. L. and Brophy, P. O. 1991. Twinning in a suckler herd. 1. Effects on performance of cows and their calves. Irish Journal of Agricultural Research 30: 1–9.Google Scholar
Mayne, C. S., McCaughey, W. J. and McEvoy, J. 1991. Practical implications of embryo transfer on dairy herd management. British Cattle Veterinary Association Meeting on cattle fertility, Reading, pp. 39–46.Google Scholar
Meat and Livestock Commission 1985. Beef carcase classification for meat traders. Technical leaflet 74 IOM 12/85.Google Scholar
Nielen, M., Schukken, Y. H., Scholl, D. T., Wilbrink, H. J. and Brand, A. 1989. Twinning in dairy cattle: A study of risk factors and effects. Theriogenology 32: 845–862.CrossRefGoogle ScholarPubMed
Patterson, D. C., Steen, R. W. J. and Kilpatrick, D. J. 1993. A comparison of growth, food efficiency and carcass characteristics of single and twin beef calves derived by embryo transfer. Animal Production 57: 81–89.Google Scholar
Polge, C., Broadbent, P. J. and Lu, K. H. 1991. Pregnancy rates following transfer of fresh or frozen bovine embryos produced in vitro. International symposium on animal biotechnology, Kyoto, japan, p. 5.Google Scholar
Porter, S. J., Owen, M. G., Page, S. J. and Fisher, A. V. 1990. Comparison of seven ultrasonic techniques for in vivo estimation of beef carcass composition with special reference to performance testing. Animal Production 51: 489–495.Google Scholar
Purchas, R. W. 1991. Effect of sex and castration on growth and composition. In Growth regulation in farm animals. Elsevier Applied Science Publishers Ltd.Google Scholar
Seideman, S. C., Cross, H. R., Oltjen, R. R. and Schanbacher, B. D. 1982. Utilization of the intact male for red meat production. A review. Journal of Animal Science 55: 826–840.CrossRefGoogle Scholar
Sinclair, K. D. 1993. Factors affecting the eating quality of bull beef from the stickler herd. A research and development report for the Meat and Livestock Commission.Google Scholar
Sinclair, K. D., Broadbent, P. J. and Hutchinson, J. S. M. 1994a. The effect of pre- and post-partum energy and protein supply on the performance of single- and twin-suckling beef cows and their calves. Animal Production 59: 379–389.Google Scholar
Sinclair, K. D., Broadbent, P. J. and Hutchinson, J. S. M. 1994b. The effect of pre- and post-partum energy and protein supply on the blood composition and reproductive performance of single- and twin-suckling beef cows. Animal Production 59: 391–400.Google Scholar
Sloss, V., Tierarzt, G. and Johnston, D. E. 1967. The causes and treatment of dystocia in beef cattle in Western Victoria. 2. Causes, methods of correction and maternal death rates. Australian Veterinary Journal 43:13–21.Google Scholar
Sreenan, J. M. and Diskin, M. G. 1989. Effect of unilateral or bilateral twin embryo distribution on twinning and embryo survival rate in the cow. Journal of Reproduction and Fertility 87: 657–664.CrossRefGoogle ScholarPubMed
Suzuki, T., Sakai, Y., Ishida, T., Matsuda, S., Miura, H. and Itoh, Y. 1989. Induction of twinning in crossbred heifers by ipsilateral frozen embryo transfer. Theriogenology 31: 917–926.CrossRefGoogle ScholarPubMed
Topps, J. H., Broadbent, P. J., Methu, J. N. and Xaba, B. B. N. 1990. The effect of different distributions of the same total dietary energy between late pregnancy and early lactation on the performance of twin-bearing suckler cows. Animal Production 50: 545 (abstr.).Google Scholar
Willadsen, S. M., Janzen, R. E., McAllister, R. J., Shea, B. F., Hamilton, G. and McDermand, D. 1991. The viability of late morulae and blastocysts produced by nuclear transplantation in cattle. Theriogenology 35:161–170.CrossRefGoogle Scholar
Wilmut, I. and Sales, D. I. 1981. The effect of an asynchronous environment on embryonic development in sheep. Journal of Reproduction and Fertility 61:179–184.CrossRefGoogle ScholarPubMed
Wright, I. A. and Russel, A. J. F. 1984. Partition of fat, body composition and body condition score in mature cows. Animal Production 38: 23–32.Google Scholar