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Immunohistochemical quantification of fast-myosin in frozen histological sections of goat limb muscles

Published online by Cambridge University Press:  02 September 2010

N. Manabe ,
Y Azuma ,
Y. Furuya ,
K. Kuramitsu ,
Y. Kuribayashi ,
N. Nagano  and
H. Miyamoto
N. Manabe
Affiliation:
Department of Animal Science, Kyoto University, Kyoto 601-01, Japan
Y Azuma
Affiliation:
Interdisciplinary Research Institute of Environmental Sciences, Kyoto 602, Japan
Y. Furuya
Affiliation:
Department of Animal Science, Kyoto University, Kyoto 601-01, Japan
K. Kuramitsu
Affiliation:
Department of Animal Science, Kyoto University, Kyoto 601-01, Japan
Y. Kuribayashi
Affiliation:
Department of Animal Science, Kyoto University, Kyoto 601-01, Japan
N. Nagano
Affiliation:
Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd, Takasaki 370-12, Japan
H. Miyamoto
Affiliation:
Department of Animal Science, Kyoto University, Kyoto 601-01, Japan
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Abstract

Fast-myosin in frozen histological sections of eight, 10, 11 and nine muscles of the upper forelimb, lower forelimb, upper hindlimb and lower hindlimb, respectively, of goats was quantified by an immunohistochemical micromethod based on the enzyme-linked immunosorbent assay. The structure of the muscles is well preserved during the immunohistochemical measurement. High fast-myosin levels (more than 201 mg/g total protein) were observed in the triceps brachii (lateral head), rectus femoris, vastus lateralis, semitendinosus, semimembranosus, gastrocnemius (lateral head) and long digital extensor muscles. In contrast, low fast-myosin levels (less than 50 mg/g) were found in the triceps brachii (medial head), superficial digital flexor, vastus intermedialis, and soleus muscles. Fast-myosin-positive fibres (type II or fast-twitch type) were distributed more in the superficial regions than in the deeper regions in the triceps brachii (lateral and long heads), biceps brachii, brachialis, biceps femoris, vastus lateralis, vastus medialis, semimembranosus and gastrocnemius (lateral and medial heads) muscles. In contrast, type IIfibres were distributed more in the deeper regions than in the superficial regions in the extensor carpi radialis, deep digital flexor, cranial tibial, deep digital flexor and superficial digital flexor muscles. When the results obtained by the immunohistochemical micromethod were compared with those obtained by biochemical techniques and by histomorphometrical analyses, high correlations were noted. This technique could be used in research projects to study the muscle characteristics that determine meat quality.

Keywords

goatsimmunohistochemistrymyofibrilsmyosinskeletal muscle
Type
Research Article
Information
Animal Science , Volume 62 , Issue 2 , April 1996 , pp. 325 - 335
Copyright
Copyright © British Society of Animal Science 1996

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