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Freezing buck semen diluted in amine-organic buffers

Published online by Cambridge University Press:  02 September 2010

S. Dunner
S. Dunner
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
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Abstract

Amine-organic buffers (BESKOH and TESTRIS) were compared for their ability to be used for buck semen dilution in a freezing procedure. BESKOH showed better results (P < 0·05) at the time of dilution and after chilling at +5°C (72% motility v. 54% at dilution and 62% v. 39% after chilling). After freezing and thawing, none of the variables measured (motility, normal acrosome ridge and normal swelling) was significantly different. A washing procedure was necessary when freezing was undertaken and egg yolk addition was necessary to avoid cold shock as the temperature lowered during chilling. There were no significant differences between a 3% and 12% level of egg yolk addition.

Keywords

amine-organic buffersdairy goatsegg yolksemen preservation
Type
Research Article
Information
Animal Production , Volume 56 , Issue 3 , June 1993 , pp. 387 - 391
Copyright
Copyright © British Society of Animal Science 1993

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