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Evaluation of milk allantoin excretion as an index of microbial protein supply in lactating dairy cows

Published online by Cambridge University Press:  02 September 2010

K. J. Shingfield  and
N. W. Offer
K. J. Shingfield
Affiliation:
Grassland and Ruminant Science Department, Scottish Agricultural College, Auchincruive, Ayr KA6 5HW
N. W. Offer
Affiliation:
Grassland and Ruminant Science Department, Scottish Agricultural College, Auchincruive, Ayr KA6 5HW
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Abstract

The potential of milk allantoin as an index ofmicrobial protein supply was evaluated in two experiments conducted with 12 multiparous Holstein-Friesian dairy cows that examined the effects of altering the supply of metabolizable energy (ME) and fermentable metabolizable energy (FME). In the first experiment, late lactation cows received a 14·3 kg dry matter (DM) per day basal diet consisting of (g/kg DM) chopped barley straw (415), soya-bean meal (322), molassed sugar-beet pulp (197), molasses (33), urea (17) and a vitamin and mineral supplement (17) for a 21-day co-variance period. During four 16-day periods, six treatments were allocated randomly to cows, consisting of potato starch (1, 2 or 3 kg DM per day) or fat supplements (0·64, 1·27 or 1·91 kg DM per day). In the second experiment, early lactation cows received 40 kg/day (fresh weight, (FW)) of silage (307 g/kg toluene-corrected dry matter, 149 g/kg DM crude protein and 11·6 MJ/kg ME). During three 21-day periods, four treatments were evaluated consisting of supplements of either 4·1 (LI) or 8·1 (12) kg fresh weight per day of a low fat concentrate (acid hydrolysis ether extract (AHEE) 45 g/kg DM) or 3·8 (HI) or 7·5 (H2) kg fresh weight per day of a high fat concentrate (AHEE 110 g/kg DM). Both experiments showed individual cow milk allantoin concentration or excretion to be poorly correlated with urinary purine derivative excretion or calculated microbial protein supply. Use of treatment mean (TM) values dramatically improved these relationships. For pooled TM (no. = 10) values from both experiments, close relationships existed between milk allantoin excretion and concentration with milk yield (r values 0·991 and 0·883, respectively). Auto-correlation with milk yield appeared to account for milk allantoin excretion and concentration being highly correlated with urinary purine derivative excretion (r values 0·908 and 0·934, respectively) and calculated microbial protein supply (r values 0·938 and 0·945, respectively). Current experimental data indicates that measurement of milk allantoin is not a reliable indicator of microbial protein supply for individual cows.

Keywords

allantoindairy cowsmicrobial proteinpurines
Type
Research Article
Information
Animal Science , Volume 67 , Issue 3 , December 1998 , pp. 371 - 385
Copyright
Copyright © British Society of Animal Science 1998

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References

Agricultural and Food Research Council. 1992. Technical Committee on Responses to Nutrients, report number 9. Nutritive requirements of ruminant animals: protein. Nutrition Abstracts and Reviews, Series B 62: 787–835.Google Scholar
Agricultural Research Council. 1984. The nutrient requirements of ruminant livestock, supplement 1. Commonwealth Agricultural Bureaux, Slough.Google Scholar
Alexander, R. H. 1969. The establishment of a laboratory procedurefor the ‘in-vitro’ determination ofdigestibility. West Scotland Agricultural College, research bulletin no. 42.Google Scholar
Antoniewicz, A., Heinemann, W. W. and Hanks, E. M. 1981. Effect of level of feed intake and body mass on allantoin excretion and the allantoin to creatinine ratio in the urine of sheep. Roczniki Naukowe Zootechniki. 8: 49–65.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. 1991. Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116: 309·317.CrossRefGoogle Scholar
Baldwin, R. L. and Smith, N. E. 1983. Adaptations of metabolism to various conditions: milk production. In Dynamic biochemistry of animal production (ed. Riis, P. M.) world animal science, volume A3, pp. 359–386. Elsevier, Amsterdam.Google Scholar
Bates, D. B., Gillett, J. A., Barao, S. A. and Bergen, W. G. 1985. The effect of specific growth rate and stage of growth on nucleic acid-protein values of pure cultures and mixed ruminal bacteria. Journal of Animal Science. 61: 713–724.CrossRefGoogle Scholar
Bikhardt, K. and Duengelhoef, R. 1994. Clinical examination of renal function in sheep. I. Methods and reference values of healthy animals. Deutsche Tierarztliche Wochenschrift. 101: 463–466.Google Scholar
Calsamiglia, S., Stern, M. D. and Firkins, J. L. 1996. Comparison of nitrogen-15 and purines as microbial markers in continuous culture. Journal of Animal Science. 74: 1375–1381.CrossRefGoogle ScholarPubMed
Carlsson, J. 1994. The value of the concentration of urea in milk as an indicator of the nutritional values of diets for dairy cows, and its relationships with milk production and fertility. Ph.D. thesis, University of Agricultural Sciences, Skara, Sweden.Google Scholar
Cecava, M. J., Merchen, N. R., Gay, L. C. and Berger, L. L. 1990. Composition of ruminal bacteria harvested from steers as influenced by dietary energy level, feeding frequency, and isolation techniques. Journal of Dairy Science. 73: 2480–2488.CrossRefGoogle ScholarPubMed
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. and Brown, D. S. 1990a. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British journal of Nutrition. 63: 131–142.CrossRefGoogle ScholarPubMed
Chen, X. B., Kyle, D. J., Ørskov, E. R. and Hovell, F. D. DeB. 1991. Renal clearance of plasma allantoin in sheep. Experimental Physiology. 76: 59–65.CrossRefGoogle ScholarPubMed
Chen, X. B., Ørskov, E. R. and Hovell, F. D. DeB. 1990b. Excretion of purine derivatives by ruminants: endogenous excretion, differences between cattle and sheep. British Journal of Nutrition. 63: 121–129.CrossRefGoogle ScholarPubMed
Chen, X. B., Susmel, P., Stefanon, B. and Ørskov, E. R. 1995. On the use of spot urine, plasma and milk samples as indicators of microbial protein supply in sheep and cattle. VII symposium on protein metabolism and nutrition, pp. 103 (abstr.).Google Scholar
Chetal, U., Mehra, U. R., Nath, K. and Ranjhan, S. K. 1975. On the variation of urinary creatinine in buffalo calves and the effect of dietary protein intake on urinary creatinine, creatinine-nitrogen ratio and creatinine coefficient. Journal of Agricultural Science, Cambridge. 84: 1–5.CrossRefGoogle Scholar
Clark, J. H., Klusmeyer, T. H. and Cameron, M. R. 1992. Microbial protein synthesis and flows of nitrogen fractions t o the duodenum of dairy cows. Journal of Dairy Science 75: 2304–2323.CrossRefGoogle Scholar
Coppock, C. E. and Wilks, D. L. 1991. Supplemental fat in high-energy rations for lactating cows: effects on intake, digestion, milk yield and composition. Journal of Animal Science. 69: 3826–3837.CrossRefGoogle ScholarPubMed
Craig, W. M., Brown, D. R., Broderick, G. A. and Ricker, D. B. 1987. Post-prandial compositional changes of fluid-and particle-associated rumen microorganisms. Journal of Animal Science. 65: 1042–1048.CrossRefGoogle Scholar
Deutsch, A. and Mattsson, S. 1959. Purine and pyrimidine derivatives in cows' milk. Fifteenth international dairy congress, volume 3, pp. 1700–1703.Google Scholar
Dewhurst, R. J., Mitton, A. M., Offer, N. W. and Thomas, C. 1996a. Effects of the composition of grass silages on milk production and nitrogen utilization by dairy cows. Animal Science. 62: 25–34.CrossRefGoogle Scholar
Dewhurst, R. J., Theobald, V. J., Neville, M. A., Miles, S. and Evans, R. T. 1996b. Relationship between the excretion of allantoin in urine and milk of dairy cows. Animal Science 62: 89 (abstr.).Google Scholar
Djouvinov, D. S. and Todorov, N. A. 1994. Influence of dry matter intake and passage rate on microbial protein synthesis in the rumen of sheep and its estimation by cannulation and a non-invasive method. Animal Feed Science and Technology. 48: 289–304.CrossRefGoogle Scholar
Emanuelson, U., Andersson, L. and Alenius, S. 1989. Milk components as routine indicators of sub-clinical diseases and use in epidemiological research. Proceedings of the Society of Veterinary Epidemiology and Medicine, pp. 117–127.Google Scholar
Faichney, G. J. and Welch, R. J. 1994. Renal excretion of allantoin and creatinine by Merino sheep selected for higher clean fleece weight. Proceedings of the Society of Nutrition Physiology, vol. 3 (ed. Giesecke, D.), pp. 113. D. L. G.-Verlag, Frankfurt.Google Scholar
Giesecke, D., Balsliemke, J., Sudekum, K. H. and Stangassinger, M. 1993. Plasma level, clearance and renal excretion of endogenous and ruminal purines in the bovine. Journal of Animal Physiology and Animal Nutrition 70: 180–189CrossRefGoogle Scholar
Giesecke, D., Ehrentreich, L., Stangassinger, M. and Ahrens, F. 1994. Mammary and renal excretion of purine metabolites in relation to energy intake and milk yield in dairy cows. Journal of Dairy Science. 77: 2376–2381.CrossRefGoogle ScholarPubMed
Givens, D. I., Adamson, A. H. and Cobby, J. M. 1988. The effect of ammoniation on the nutritive value of wheat, barley and oat straws. II. Digestibility and energy value measurements in vivo and their prediction from laboratory methods. Animal Feed Science and Technology. 19: 173–184.CrossRefGoogle Scholar
Gonda, H. L. 1995. Nutritional status of ruminants determined from excretion and concentration of metabolites in body fluids. Ph.D. thesis, Swedish University of Agricultural Sciences, Uppsala, Sweden.Google Scholar
Gonda, H. L. and Lindberg, J. E. 1994. Evaluation of dietary nitrogen utilisation in dairy cows based on urea concentrations in blood, urine and milk, and on urinary concentrations of purine derivatives. Ada Agricultse Scandinavica, Section A, Animal Science. 44: 236–245.Google Scholar
Greger, R., Lang, F. and Deetjen, P. 1976. Renal excretion of purine metabolites, urate and allantoin by the mammalian kidney. In International review of physiology: kidney and urinary tract physiology II, volume II (ed. Thurau, K.), pp. 257–281.Google Scholar
Grovum, W. L. and Williams, V. J. 1973. Rate of passage of digesta in sheep. 4. Passage of marker through the alimentary tract and the biological relevance of rate-constants derived from changes in concentration of marker i n faeces. British Journal of Nutrition. 30: 313–329.CrossRefGoogle Scholar
Gustafsson, A. H. 1993. Acetone and urea concentrations in milk as indicators of the nutritional status and the composition of the diet of dairy cows. Ph.D. thesis, University of Agricultural Sciences, Uppsala, Sweden.Google Scholar
John, A. and Ulyatt, M. 1984. Measurement of protozoa, using phosphatidyl choline, and of bacteria using nucleic acid, in the duodenal digesta of sheep fed chaffed lucerne hay (Medicago sativa L.) diets. Journal of Agricultural Cambridge. 102: 33–44.CrossRefGoogle Scholar
Kirchgessner, M. and Kaufmann, T. E. G. 1987. Urea and allantoin in the milk of cows during and after excess of energy supply. 5. Communications on the influence of excessive energy supply of lactating cows and its consequences. Journal of Animal Physiology and Animal Nutrition. 58: 147–156.CrossRefGoogle Scholar
Kirchgessner, M. and Kreuzer, M. 1985. Urea and allantoin n i the milk of cows during and after feeding protein in excess or protein deficiency. 5. The influence of incorrect protein nutrition of lactating cows and its residual effects. Journal of Animal Physiology and Animal Nutrition 54: 141–151.Google Scholar
Kirchgessner, M. and Windisch, W. 1989. Milk urea content and allantoin excretion of cows during and after energy and protein depletion. The influence of energy and protein depletion and its residual effects. Journal of Animal Physiology and Animal Nutrition. 62: 113–118.CrossRefGoogle Scholar
Lawes Agricultural Trust. 1993. GENSTAT 5 reference manual Clarendon Press, Oxford.Google Scholar
Lebzien, V. P., Giesecke, D., Wiesmayr, S. and Rohr, K. 1993. Measurement of microbial protein synthesis in the rumen of cows by 15N determination in duodenal contents and excretion of allantoin in the milk. Journal of Animal Physiology and Animal Nutrition. 70: 82–88.CrossRefGoogle Scholar
McDonald, P., Edwards, R. A. and Greenhalgh, J. F. D. 1981. Animal nutrition, third edition. Longman, Harlow, Essex.Google Scholar
Martin Orúe, S. M., Dapoza, C., Balcells, J. and Castrillo, C. 1996. Purine derivatives excretion in lactating ewes fed straw diets with different levels of fish meal. Animal Feed Science and Technology. 63: 341–346.CrossRefGoogle Scholar
Merry, R. J. and McAllan, A. B. 1983. A comparison of the chemical composition of mixed rumen bacteria harvested form the liquid and solid fractions of rumen digesta. British Journal of Nutrition. 50: 701–709.CrossRefGoogle ScholarPubMed
Ministry of Agriculture, Fisheries and Food. 1975. Energy allowances and feeding systems for ruminants. Technical bulletin no. 33. Ministry of Agriculture, Fisheries and Food, London.Google Scholar
Minitab Inc. 1980. Minitab data analysis software. Pennsylvania State University, Pennsylvania.Google Scholar
Motojima, K. and Goto, S. 1990. Characteristics of liver-specific expression of rat uricase using monoclonal antibodies and cloned cDNAs. Biochimica et Biophysica Ada 1087: 316.CrossRefGoogle Scholar
Narayanan, S. and Appleton, H. D. 1980. Creatinine: a review. Journal of Clinical Chemistry. 26: 1119–1126.CrossRefGoogle ScholarPubMed
Offer, N. W., Cottrill, B. R. and Thomas, C. 1996. The relationship between silage evaluation and animal response. Proceedings of the 11th silage conference, Aberyshpyth, UK, pp. 26–38.Google Scholar
Offer, N. W., Rooke, J. A., Dewhurst, R. J. and Thomas, C. 1993. Rapid assessment of silage fermentation characteristics by electrometric titration. Animal Production 56: 423 (abstr.).Google Scholar
Orúe, S. M. M., Dapoza, C., Balcells, J. and Castrillo, C. 1996. Purine derivatives excretion in lactating ewes fed straw diets with different levels of fish meal. Animal Teed Science and Technology. 63: 341–346.CrossRefGoogle Scholar
Falmquist, D. L. 1984. Use of fats in diets for lactating dairy cows. In Fats in animal nutrition (ed. Wiseman, J.), pp. 357–381. Butterworths, London.CrossRefGoogle Scholar
Patterson, H. D. and Thompson, R. 1971. Recovery of interblock-information when block sizes are unequal. Biometrika. 58: 545–554.CrossRefGoogle Scholar
Perez, J. F., Balcells, J., Guada, J. A. and Castrillo, C. 1996a. Determination of rumen nitrogen production in sheep: a comparison of urinary purine derivative excretion with methods using 15N and purine bases as markers of microbial-nitrogen entering the duodenum. British Journal of Nutrition. 75: 699–709.Google ScholarPubMed
Perez, J. F., Rodriguez, C. A., Gonzalez, J., Balcells, J. and Guada, J. A. 1996b. Contribution of dietary purine bases to duodenal digesta in sheep. In situ studies of purine degradability corrected for microbial contamination. Animal Feed Science and Technology. 62: 251–262.CrossRefGoogle Scholar
Ropstad, E., Vik-Mo, L. and Refsdal, A. O. 1989. Levels of milk urea, plasma constituents and rumen liquid ammonia in relation to feeding of dairy cows during early lactation. Acta Veterinaria Scandinavica. 30: 199–208.CrossRefGoogle ScholarPubMed
Rosskopf, R. and Giesecke, D. 1992. Investigations in cows on the influence of energy intake on rumen metabolism by means of allantoin excretion in the milk. Journal of Veterinary Medicine, Series A. 39: 515–524.CrossRefGoogle Scholar
Shingfield, K. J. and Offer, N. W. 1998a. Determination of allantoin in bovine milk by high performance liquid chromatography. Journal of Chromatography B. 706: 342–346.CrossRefGoogle ScholarPubMed
Shingfield, K. J. and Offer, N. W. 1998b. Evaluation of the spot urine sampling technique to assess urinary purine derivative excretion in lactating dairy cows. Animal Science. 66: 557–568.CrossRefGoogle Scholar
Smith, R. H., McAllan, A. B., Hewitt, D. and Lewis, P. E. 1978. Estimation of amounts of microbial and dietary nitrogen compounds entering the duodenum of cattle. Journal of Agricultural Science, Cambridge 90: 557–568.CrossRefGoogle Scholar
Storry, J. E. 1981. The effect of dietary fat on milk composition. In Recent advances in animal nutrition (ed. , Haresign), pp. 3–33. Butterworths, London.CrossRefGoogle Scholar
Surra, J. C., Guada, J. A., Balcells, J. and Castrillo, C. 1997. Renal and salivary clearance of purine derivatives in sheep. Animal Science 65: 83–91.CrossRefGoogle Scholar
Susmel, P., Stefanon, B., Plazzotta, C. R., Spanghero, M. and Mills, C. R. 1994. The effect of energy and protein intake on the excretion of purine derivatives. Journal of Agricultural Science, Cambridge. 123: 257–265.CrossRefGoogle Scholar
Thomas, P. C., Robertson, S., Chamberlain, D. G., Livingstone, R. M., Garthwaite, P. H., Dewey, P. J. S., Smart, R. and Whyte, C. 1988. Predicting the metabolisable energy (ME) content of compound feeds for ruminants. In Recent advances in animal nutrition (ed. Haresign, W. and Cole, D. J. A.), pp. 127–146. Butterworths, London.Google Scholar
Tiermeyer, W., Stohrer, M. and Giesecke, D. 1984. Metabolites of nucleic acids in bovine milk. Journal of Dairy Science. 67: 723–728.CrossRefGoogle Scholar
Tilley, J. M. A. and Terry, R. A. 1963. A two-stage technique for the in-vitro digestion of forage crops. Journal the British Grassland Society 18: 104.CrossRefGoogle Scholar
Topps, R. H. and Elliot, R. C. 1965. Relationship between concentrations of ruminal nucleic acid and excretion of purine derivatives by sheep. Nature. 205: 498–499.CrossRefGoogle Scholar
Uden, P., Colucci, P. E. and Van Soest, P. J. 1980. Investigation of chromium, cerium and cobalt as markers in digesta rate of passage studies. Journal of the Science of and Agriculture. 31: 625–632.Google ScholarPubMed
Vagnoni, D. B., Broderick, G. A., Clayton, M. K. and Hatfield, R. D. 1997. Excretion of purine derivatives by Holstein cows abomasally infused with incremental amounts of purines. Journal of Dairy Science. 80: 1695–1702.CrossRefGoogle ScholarPubMed
Verbic, J., Chen, X. B., Macleod, N. A. and Ørskov, E. R. 1990. Effect of microbial nucleic acids infusion on purine derivative excretion by steers. Journal of Agricultural Science, Cambridge. 114: 243–248.CrossRefGoogle Scholar
Young, E. G. and Conway, C. F. 1942. On the estimation of allantoin by Rimini-Schryver reaction. Journal of Biological Chemistry. 142: 839–852.CrossRefGoogle Scholar