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Evaluation of intrauterine insemination of sheep with frozen semen: effects of time of insemination and semen dose on conception rates

Published online by Cambridge University Press:  02 September 2010

R. C. F. Findlater
Affiliation:
University of Nottingham School of Agriculture, Sutton Bonington, Loughborough LE12 5RD
W. Haresign
Affiliation:
University of Nottingham School of Agriculture, Sutton Bonington, Loughborough LE12 5RD
R. M. Curnock
Affiliation:
MLC Pig Breeding Centre, Field Lane, Thorpe Willoughby, Selby YO8 9NL
N. F. G. Beck
Affiliation:
Department of Agriculture, University College of Wales, Aberystwyth SY23 3DD
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Abstract

The field application of a laparoscopic technique to permit intra-uterine insemination of ewes with frozen-thawed semen was examined in two trials, conducted over successive years, to (i) determine the optimum time of insemination relative to sponge removal/pregnant mares's serum gonadotropin (PMSG) injection and (ii) establish the relationship between semen dose and conception rate. Pooled semen was used in both trials, and each involved > 900 ewes in a number of commercial flocks.

Maximum conception rates were achieved when insemination was conducted between 54 h and 60 h after sponge removal/ PMSG injection. However, there was no significant difference in conception rate when motile sperm numbers were reduced from 52·2 × 106to 13·0 × 106 motile sperm per uterine horn.

The overall conception rates (pooled over flocks and treatment groups) were 56% and 58% for the two trials, with a wide degree of variation between flocks in both cases (45% to 79% for trial 1 and 45% to 69% for trial 2). However, there was evidence for consistent differences in conception rates between the six flocks involved in both years of the trials.

Type
Research Article
Copyright
Copyright © British Society of Animal Science 1991

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