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Electron spin resonance of wool from Merino sheep heterozygous or homozygous for white fleece

Published online by Cambridge University Press:  02 September 2010

M. R. Fleet ,
S. F. Lincoln  and
A. M. Hounslow
M. R. Fleet
Affiliation:
Department of Agriculture, Animal Research Branch, Box 1671 GPO, Adelaide 5001, Australia
S. F. Lincoln
Affiliation:
University of Adelaide, Department of Physical and Inorganic Chemistry, Box 498 GPO, Adelaide 5001, Australia
A. M. Hounslow
Affiliation:
University of Adelaide, Department of Physical and Inorganic Chemistry, Box 498 GPO, Adelaide 5001, Australia
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Abstract

Wool samples were collected from two groups of Merino sheep, both of which contained animals that were either heterozygous (Ww) or homozygous (WW) for white fleece. Group 1 were sampled in summer and group 2 in winter. These samples were scoured and measured for electron spin resonance initially (ESR1), following irradiation by ultraviolet light (ESR2) and then after wetting and drying the sample (ESR3).

There was no significant difference between WW and Ww sheep in each group for any of the ESR measurements (P > 0·05). However, for ESR1 there was a difference between management groups (group 1> group 2; P < 0·05). The ESR3 on ESR1 values were plotted separately for WW and Ww genotypes. The plotted ESR3 on ESR1 values in both groups showed no differentation into distinct genotype clusters. Nevertheless, for group 2 there was a slight similarity to the cross-like pattern of genotypes reported in an earlier description of this technique and the regression coefficients of ESR3 on ESR1 for WW sheep (b = −0·09 (s.e. 0·28)) differed significantly from those of Ww sheep (b = 1·31 (s.e. 0·52)) (P < 0·05).

It appears that the previously published method for differentiating between sheep that are either heterozygous or homozygous for white fleece was ineffective in this case.

Type
Research Article
Information
Animal Production , Volume 47 , Issue 1 , August 1988 , pp. 97 - 103
Copyright
Copyright © British Society of Animal Science 1988

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References

Adalsteinsson, S. 1974. Colour inheritance in farm animals and its application in selection. Proceedings of the 1st World Congress on Genetics Applied to Livestock Production, Madrid, Vol. 1, pp. 29–37.Google Scholar
Australian Wool Corporation. 1987. Australian Wool Sale Statistics 1987. Statistical Analysis No. 17B.Google Scholar
Body, E. M., Meager, J., Pennefather, H. F. and Simpson, G. K. 1962. Black or brown spots on Merinos: Are they important? Pastoral Review 72: 621–622.Google Scholar
Brooker, M. G. 1968. Pigmentation in the Australian Merino. M. Agric. Sc. Thesis, University of Sydney.Google Scholar
Brooker, M. G. and Dolling, C. H. S. 1969. Pigmentation of sheep: II. The inheritance of colour patterns in black Merinos. Australian Journal of Agricultural Research 20: 387–394.CrossRefGoogle Scholar
Dolling, C. H. S. 1984. The coloured sheep industry in Australia. Proceedings of the World Congress on Coloured Sheep Products, New Zealand, pp. 21–27. New Zealand Black and Coloured Sheep Breeders Association.Google Scholar
Eaton, W. C. and Keighley, J. H. 1971. Free radicals induced in wools by x-rays. Applied Polymer Symposia 18: 263–268.Google Scholar
Fleet, M. R. 1985. Pigmented fibres in white wool. Wool Technology and Sheep Breeding 33: 5–13, 27.Google Scholar
Fleet, M. R. and Lincoln, S. F. 1984. Electron spin resonance of wool from melanin-pigmented sheep and sheep heterozygous or homozygous for white wool. Australian Association of Animal Breeding and Genetics 4: 213–215.Google Scholar
Fleet, M. R., Stafford, J. E., Dawson, K. A. and Dolling, C. H. S. 1986. Contamination of white wool by melanin-pigmented fibres when pigmented and white sheep graze together. Australian Journal of Experimental Agriculture 26: 159–163.CrossRefGoogle Scholar
Freund, R. J. and Littel, R. C. 1981. A Guide to the ANOVA and GLM Procedures. Statistical Analysis Systems Institute, Cary, NC.Google Scholar
Mason, H. S., Ingham, D. J. E. and Allen, B. 1960. The free radical property of melanins. Archives of Biochemistry and Biophysics 86: 225–230.CrossRefGoogle ScholarPubMed
“Old Hand”. 1953. Black or brown spots on non-wool parts of a Merino sheep. Reprinted in Pastoral Review, 1962, 72: 464–465.Google Scholar
Palithorpe, M. T. and Nichols, C. H. 1972. ESR studies of the low temperature irradiation of keratin and its component amino acids. Photochemistry and Photobiology 15: 465–477.CrossRefGoogle Scholar
Pathak, M. A. and Stratton, K. 1968. Free radicals in human skin before and after exposure to light. Archives of Biochemistry and Biophysics 123: 468–476.CrossRefGoogle ScholarPubMed
Sacchi, S., Lanzi, G. and Zonotti, L. 1969. Electron spin resonance research on human hairs under varying physiological and experimental conditions. Advances in Biology of the Skin 9: 169–175.Google Scholar
Shatkay, A. and Michaeli, I. 1970. Electron paramagnetic resonance study of wool irradiated by ultraviolet and visible light. Radiation Research 43: 485–498.CrossRefGoogle ScholarPubMed
Turner, H. N., Hayman, R. H., Riches, J. H., Roberts, N. F. and Wilson, L. T. 1953. Physical definition of sheep and their fleece for breeding and husbandry studies. Report, Division of Animal Health and Production, Commonwealth Scientific and Industrial Research Organization, Series SW2, No. 4.Google Scholar
Vsevolodov, E. B., Latypov, I. F., Martynov, Yu. F. and Razozanev, K. M. 1981. Detection of sheep heterozygous for dominant white by electron paramagnetic resonance spectrometry. Trudy Instituta Eksperimentalńoi Biologii Akademiya Nauk Kazakhskoi SSR, 15: 61–73.Google Scholar
Vsevolodov, E. B., Latypov, I. F. and Ryabikin, Yu. A. 1974. Study of wool pigmentation by the method of EPR-spectrography. Set'skokhozyaistvennaya Biologiya 9: 295–301.Google Scholar
Windle, J. J. 1962. Naturally occurring electron paramagnetic resonance in wool. Textile Research Journal 32: 963–965.CrossRefGoogle Scholar