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The effects of synchronizing the rate of dietary energy and nitrogen supply to the rumen on the production and metabolism of sheep: food characterization and growth and metabolism of ewe lambs given food ad libitum

Published online by Cambridge University Press:  18 August 2016

M. W. Witt ,
L. A. Sinclair ,
R. G. Wilkinson  and
P. J. Buttery
M. W. Witt
Affiliation:
School of Agriculture, Harper Adams University College, Edgmond, Newport TF10 8NB
L. A. Sinclair
Affiliation:
School of Agriculture, Harper Adams University College, Edgmond, Newport TF10 8NB
R. G. Wilkinson
Affiliation:
School of Agriculture, Harper Adams University College, Edgmond, Newport TF10 8NB
P. J. Buttery
Affiliation:
University of Nottingham, Division of Nutritional Biochemistry, School of Biological Sciences, Sutton Bonington Campus, Loughborough LE12 5RD
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Abstract

The effects of diets formulated to have two rates of organic matter (OM) release and to be either synchronous or asynchronous with respect to their hourly release of nitrogen (N) and OM in the rumen on the growth, intake and metabolism of ewe lambs was studied. In experiment 1, the in situ degradation characteristics of N and OM for 16 food ingredients was determined. The foods varied considerably in their chemical composition and degradability coefficients of N and OM. Based on this information, four diets were formulated to differ in their rate of N and OM release in the rumen but to have a similar content of metabolizable energy (10·4 MJ/kg dry matter (DM)), crude protein (140 g/kg DM), daily ratio of N: OM released (34 g N per kg OM) and digestible undegradable protein (32 g/kg DM) but to be synchronous or asynchronous with respect to hourly N: OM ratio. The four diets were slow energy, synchronous (SS), slow energy asynchronous (SA), fast energy, synchronous (FS) and fast energy asynchronous (FA).

In experiment 2 the four diets were offered ad libitum to 24 ewe lambs of an initial live weight of 25 kg in a 2 × 2 factorial design. Lambs were slaughtered at 40 kg live weight. Animals offered diets FS and FA had a higher growth rate than those offered diets SA and SS (266 v. 225 g/day respectively; P < 0·05). There was a significant interaction between rate ofOM and N release on DM intake (DMI) with lambs offered diet FS consuming less than those offered FA (1·47 v. 1·67 kg DM per day; P < 0·05). Rate of energy release and synchrony had an effect on food conversion efficiency (FCE; kg gain per kg DMI) with lambs offered diets FS and FA having a greater FCE than those offered SS and SA (0·170 v. 0·146 respectively; P < 0·001) and those offered diets SS and FS had a greater FCE than those offered diets SA and FA (0·164 v. 0·152 respectively; P < 0·05). Diet had little effect on carcass composition. The proportion of propionate in rumen fluid was greater in lambs offered diets FS and FA than in those offered diets SS and SA (222 v. 168 mmol/mol respectively; P < 0·01). Plasma urea concentrations exhibited a cyclical trend throughout the day with highest concentrations occurring 2 h after fresh food was offered. Lambs offered diet FS had lower plasma urea concentrations at 6, 10 and 14 h after feeding. Plasma concentrations of ß-hydroxybutyrate were lower throughout the day in lambs offered diet FS. The current findings are consistent with the view that a rapid release of OM and synchronizing hourly N: OM release in the rumen can improve the efficiency of growth through improvements in protein and/or energy metabolism.

Keywords

energy intakeproteingrowthlambsrumen digestion
Type
Research Article
Information
Animal Science , Volume 69 , Issue 1 , August 1999 , pp. 223 - 235
Copyright
Copyright © British Society of Animal Science 1999

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Footnotes

†

Present address: Trident Feeds, PO Box 11, Oundle Road, Peterborough PE2 9QX.

References

Agricultural and Food Research Council. 1992. Technical Committee on Responses to Nutrients. Report no. 9. Nutritive requirements of ruminant animals: protein. Nutrition Abstracts and Reviews, Series B: Livestock Feeds and Feeding 62: 787–835.Google Scholar
Akin, D. E. 1988. Biological structure of lignocellulose and its degradation in the rumen. Animal Feed Science and Technology 21: 295–310.Google Scholar
Association of Official Analytical Chemists. 1980. Official methods of analysis, 13th edition. Association of Official Analytical Chemists, Washington, DC. Google Scholar
Blackburn, W. E., Sinclair, L. A., Wilkinson, R. G. and Greenhalgh, J. F. D. 1998. Influence of the pattern of nutrient release from grass silage and the effect of supplementation on the voluntary food intake of growing lambs. Proceedings of the British Society of Animal Science, 1998, 95 (abstr.).CrossRefGoogle Scholar
Brown, A. J. and Williams, D. R. 1979. Sheep carcass evaluation — measurements of composition using a standardised butchery method. Memo no. 38, Meat Research Institute, Bristol, UK. Google Scholar
Chamberlain, D. G. and Choung, J. J. 1995. The importance of rate of ruminal fermentation of energy sources in diets for dairy cows. In Recent advances in animal nutrition (ed. Garnsworthy, P. C. and Cole, D. J. A.), pp. 3–27. Nottingham University Press.Google Scholar
Chen, G., Russell, J. B. and Sniffen, C. J. 1987. A procedure for measuring peptides in rumen fluid and evidence that peptide uptake can be a rate-limiting step in ruminai protein degradation. Journal of Dairy Science 70: 1211–1219.Google Scholar
Cheng, K. J., Stewart, C. S., Dinsdale, D. and Costerton, J. W. 1984. Electron microscopy of bacteria involved in the digestion of plant cell walls. Animal Feed Science and Technology 10: 93–100.CrossRefGoogle Scholar
Chesson, A. 1986. The evaluation of dietary fibre. In Feedingstuffs evaluation, modern aspects, problems, future trends (ed. Livingstone, R. M.), pp. 1–10. Rowett Research Institute, Aberdeen, UK.Google Scholar
Chesson, A. 1990. Nutritional significance and nutritive value of plant polysaccharides. In Feedstuffs evaluation (ed. J. Wiseman and D. Cole, J. A.), pp. 179–195. Butterworths, London.CrossRefGoogle Scholar
Cocimano, M. R. and Leng, R. A. 1967. Metabolism of urea in sheep. British Journal of Nutrition 21: 353–371.Google Scholar
Cone, J. W., Cliné-Theil, W., Malestein, A. and Klooster, A. Th. van’t. 1989. Degradation of starch by incubation with rumen fluid. A comparison of different starch sources. Journal of the Science of Food and Agriculture 49: 173–183.CrossRefGoogle Scholar
Emmanuel, B. 1980. Oxidation of butyrate to ketone bodies and C02 in the rumen epithelium, liver, kidney, heart and lung of Camel (Camelus dromedarius), sheep (Ovis aries) and goat (Capra hircus) . Comparative Biochemistry and Physiology 65B: 699–704.Google Scholar
Forbes, J. M. 1995. Voluntary food intake and diet selection in farm animals. CAB International, Wallingford, UK.Google Scholar
Galbraith, H., Mandebvu, P., Thompson, J. K. and Franklin, M. F. 1989. Effects of diets differing in the proportion of sugar-beet pulp and barley on growth, body composition and metabolism of entire male lambs. Animal Production 48: 652 (abstr.).Google Scholar
Goering, H. K. and Van Soest, P. J. 1970. Forage fiber analysis (apparatus, reagents, procedures and some applications). Agricultural handbook no. 379, Agricultural Research Service, US Department of Agriculture, Washington DC.Google Scholar
Henning, P. H., Steyn, D. G. and Meissner, H. H. 1993. Effect of synchronisation of energy and nitrogen supply on ruminai characteristics and microbial growth Journal of Animal Science 71: 2516–2528.Google Scholar
Herrera-Saldana, R.E, Huber, J. T. and Poore, M. H. 1990. Dry matter, crude protein, and starch degradability of five cereal grains. Journal of Dairy Science 73: 2386–2393.CrossRefGoogle Scholar
Huntingdon, J. A. and Givens, I. 1995. The in situ technique for studying the rumen degradation of feeds: a review of the procedure. Nutrition Abstracts and Reviews, Series B 65: 63–93.Google Scholar
Hussein, H. S., Jordan, R. M. and Stern, M. D. 1991. Ruminai protein metabolism and intestinal amino acid utilization as affected by dietary protein and carbohydrate sources in sheep. Journal of Animal Science 69: 2134–2146.CrossRefGoogle Scholar
Johnson, R. R. 1976. Influence of carbohydrate solubility on non-protein nitrogen utilization in the ruminant. Journal of Animal Science 43: 184–191.Google ScholarPubMed
Kandylis, K. and Nikokyris, P. 1991. Reassessment of the nylon bag technique. World Review of Animal Production 26: 23–32.Google Scholar
Kyriazakis, I. and Oldham, J. D. 1997. Food intake and diet selection in sheep: the effect of manipulating the rates of digestion of carbohydrates and protein of the foods offered as a choice. British Journal of Nutrition 77: 243–254.CrossRefGoogle ScholarPubMed
Lawes Agricultural Trust. 1987. Genstat 5 reference manual. Oxford University Press, Oxford.Google Scholar
Lindsay, D. B. 1993. Metabolism of the portal drained viscera. In Quantitative aspects of ruminant digestion and metabolism (ed. Forbes, J. M. and France, J.), pp. 267–289. CAB International, Wallingford, UK.Google Scholar
Lobley, G. E., Connell, A., Lomax, M. A., Brown, D. S., Milne, E., Calder, A. G. and Farningham, D. A. H. 1995. Hepatic detoxification of ammonia in the ovine liver: possible consequences for amino acid catabolism. British Journal of Nutrition 73: 667–685.Google ScholarPubMed
McCarthy, R. D. Jr, Klusmeyer, T. H., Vivini, J. L., Clark, J. H. and Nelson, D. R. 1989. Effects of source of protein and carbohydrate on ruminai fermentation and passage of nutrients to the small intestine of lactation cows. Journal of Dairy Science 72: 2002–2016.CrossRefGoogle Scholar
MacRae, J. C. and Lobley, G. E. 1986. Interactions between energy and protein. In Control of digestion and metabolism in ruminants (ed. L. P., Milligan, Grovum, W. L. and Dobson, A.), pp. 367–385. Prentice Hall, Englewood Cliffs, New Jersey.Google Scholar
MacRae, J. C., Walker, A., Brown, D. and Lobley, G. E. 1993. Accretion of total protein and individual amino acids by organs and tissues of growing lambs and the ability of nitrogen balance techniques to quantitate protein retention. Animal Production 57: 237–245.Google Scholar
Matras, J., Bartle, S. J. and Preston, R. L. 1991. Nitrogen utilization in growing lambs: effects of grain (starch) and protein sources with various rates of ruminai degradation. Journal of Animal Science 69: 339–347.Google Scholar
Ministry of Agriculture, Fisheries and Food. 1981. Reference booklet 427. Her Majesty’s Stationery Office, London.Google Scholar
Nocek, J. E. 1988. In situ and other methods to estimate ruminai protein and energy digestibility: a review. Journal of Dairy Science 71: 932–945.Google Scholar
Nocek, J. E. and Russell, J. B. 1988. Protein and energy as an integrated system. Relationship of ruminai protein and carbohydrate availability to microbial protein synthesis and milk production. Journal of Dairy Science 71: 2070–2107.Google Scholar
Oldham, J. D. 1987. Testing and implementing modern systems: UK. In Protein metabolism of ruminant feeds (ed. Alderman, G. and Jarrige, R.), pp. 261–281. Commission of European Communities, Luxembourg.Google Scholar
Ørskov, E. R. and Allen, D. M. 1966. Utilization of salts of volatile fatty acids by growing sheep. I. Acetate, propionate and butyrate as sources of energy for young growing lambs. British Journal of Nutrition 20: 295–305.Google ScholarPubMed
Ørskov, E. R., Grubb, D. A., Smith, J. S., Webster, A. J. F. and Corrigall, W. 1979. Efficiency of utilization of volatile fatty acids for maintenance and energy retention by sheep. British Journal of Nutrition 41: 541–551.Google ScholarPubMed
Ørskov, E. R. and McDonald, I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, Cambridge 92: 499–503.Google Scholar
Ørskov, E. R. and MacLeod, N. A. 1990. Dietary induced thermogenesis and feed evaluation in ruminants. Proceedings of the Nutrition Society 49: 227–237.CrossRefGoogle ScholarPubMed
Pérez, J. F., Balcells, J., Guada, J. A. and Castrillo, C. 1997. Contribution of dietary nitrogen and purine bases to the duodenal digesta: comparison of duodenal and polyester-bag measurements. Animal Science 65: 237–245.CrossRefGoogle Scholar
Pethick, D. W. and Dunshea, F. R. 1993. Fat metabolism and turnover. In Quantitative aspects of ruminant digestion and metabolism (ed. Forbes, J. M. and France, J.), pp. 291–311. CAB International, Wallingford, UK.Google Scholar
Rooney, L. W. and Pflugfelder, R. L. 1986. Factors affecting starch digestibility with special emphasis on sorghum and corn. Journal of Animal Science 63: 1607–1623.Google ScholarPubMed
Russell, J. B. 1986. Heat production by ruminal bacteria in continuous cultura and its relationship to maintenance energy. Journal of Bacteriology 168: 694–701.CrossRefGoogle Scholar
Ryan, P. J. 1980. Determination of volatile fatty acids and some related compounds in ovine fluid, urine and blood by gas liquid chromatography. Analytical Biochemistry 108: 374–384.CrossRefGoogle Scholar
Siddons, R. C., Paradine, J., Gale, D. L. and Evans, R. T. 1985. Estimation of the degradability of dietary protein in the sheep by in vivo and in vitro procedures. British Journal of Nutrition 54: 545–561.Google ScholarPubMed
Sinclair, L. A., Galbraith, H. and Scaife, J. R. 1991. Effect of dietary protein concentration and cimaterol on growth and body composition of entire male lambs. Animal Feed Science and Technology 34:181–192.Google Scholar
Sinclair, L. A., Garnsworthy, P. C., Newbold, J. and Buttery, P. J. 1993. Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep. Journal of Agricultural Science, Cambridge 120: 251–263.Google Scholar
Sinclair, L. A., Garnsworthy, P. C., Newbold, J. and Buttery, P. J. 1995. Effects of synchronizing the rate of dietary energy and nitrogen release in diets with a similar carbohydrate composition on rumen fermentation and microbial protein synthesis in sheep. Journal of Agricultural Science, Cambridge 124: 463–472.Google Scholar
Sinclair, L. A. and McQuiston, G. C. 1995. Effects of level of concentrate on the eating behaviour and predicted hourly release of nutrients in the rumen of sheep fed grass silage. Animal Science 60: 531 (abstr.).Google Scholar
Sniffen, C. J., Russell, J. B. and Van Soest, P. J. 1983. The influence of carbon source, nitrogen source and growth factors on rumen microbial growth. In Proceedings of the Cornell nutrition conference, pp. 26–33. Ithaca, New York.Google Scholar
Stouthamer, A. H. and Bettenhaussen, C. 1973. Utilization of energy for growth and maintenance in continuous culture and batch cultures of micro-organisms. Biochemica et Biophysica Acta 301: 53–70.CrossRefGoogle Scholar
Tamminga, S., Vuuren, A. M. van, Koelen, C. J. van der, Ketelaar, R. S. and Togt, P. L. van der. 1990. Ruminal behaviour of structural carbohydrates, non-structural carbohydrates and crude protein from concentrate ingredients in dairy cows. Netherlands Journal of Agricultural Science 38: 513–526.CrossRefGoogle Scholar
Varvikko, Y. and Lindberg, J. E. 1985. Estimation of microbial nitrogen in nylon-bag residues by feed 15N dilution. British Journal of Nutrition 54: 473–481.Google ScholarPubMed
Wainman, F. W., Dewey, P. J. S. and Boyne, A. W. 1981. Compound feedingstuffs for ruminants. Third report of Feedingstuffs Evaluation Unit. Department of Agriculture, Fisheries and Food, Edinburgh.Google Scholar
Wallace, R. J. 1996. Biphasic breakdown of peptides by rumen bacteria. Animal Science 62: 687 (abstr.).Google Scholar