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The effects of retrogradation and amylose/amylopectin ratio of starches on carbohydrate fermentation and microbial populations in the porcine colon

Published online by Cambridge University Press:  18 August 2016

C.-A. Reid  and
K. Hillman
C.-A. Reid
Affiliation:
Microbiology Unit, Animal Biology Division, Scottish Agricultural College, Ferguson Building, Craibstone, Aberdeen AB21 9YA Robert Gordon University, School of Food and Consumer Studies, Queens Road, Aberdeen AB9 2PG
K. Hillman
Affiliation:
Microbiology Unit, Animal Biology Division, Scottish Agricultural College, Ferguson Building, Craibstone, Aberdeen AB21 9YA
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Abstract

The colon of the simple-stomached animal comprises a gradually changing microbial environment, using the residues of digestion as substrates for fermentation. As carbohydrate sources decline along the length of the colon, the microflora degrade proteins to use as an energy supply, resulting in the formation of undesirable and potentially toxic metabolites. This study has examined the possibility of using dietary starches as a means of extending carbohydrate fermentation along the colon in order to reduce the effects of protein degradation, and the influence of these starches on the numbers of microbial groups within the total population. The study was carried out in weaned piglets, using native and retrograded forms of maize and waxy maize starches, containing proportionately about 0·25 and 0·01 amylose respectively. It was found that protein degradation in the mid and distal regions of the colon was best reduced by the inclusion of the retrograded form of waxy maize, although these data did not achieve significance. However, it was found that a reduced population of the protein-degrading Bacteroides spp. resulted from the inclusion of waxy maize rather than maize in the diets, (proximal colon P < 0·01 ; mid colon P < 0·001 , distal colon P < 0·05), while the retrograded waxy maize produced increased lactobacillixoliform ratios throughout the colon, which may result in an improved resistance to infection by intestinal pathogens. Retrogradation of these starches reduced the coliform populations throughout the colon, with significant (P < 0·05) effects observed in the proximal and distal regions. The data suggest that, of the starches tested, reduced protein-degrading activity and improved pathogen resistance may be achieved with retrograded waxy maize.

Keywords

amylose/amylopectincolonfermentationpigletsstarch
Type
Research Article
Information
Animal Science , Volume 68 , Issue 3 , April 1999 , pp. 503 - 510
Copyright
Copyright © British Society of Animal Science 1999

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References

Asp, N.-G. and Bjork, I. 1992. Resistant starch. Trends in Food Science and Technology 3: 111–114.CrossRefGoogle Scholar
Benkerroum, N., Ghouati, Y., Sandine, W. E. and Tantaoui-Elakari, A. 1993. Methods to demonstrate the bactericidal activity of bacteriocins. Letters in Applied Microbiology 17: 78–81.Google Scholar
Biliaderis, C. G. 1991. The structure and interactions of starch with food constituents. Canadian Journal of Physiology and Pharmacology 69: 60–78.CrossRefGoogle ScholarPubMed
Clarke, R. T. J. 1977. The gut and its micro-organisms. In Microbial ecology of the gut (ed. Clarke, R. T. J. and Bau chop, T.), pp. 35–71. Academic Press, New York.Google Scholar
Cummings, J. H. and Englyst, H. N. 1987. Fermentation in the human large intestine and the available substrates. American Journal of Clinical Nutrition 45: 1243–1255.CrossRefGoogle ScholarPubMed
Cummings, J. H. and Englyst, H. N. 1992. Complex carbohydrates. In The contribution of nutrition to human and animal health (ed. Widdowson, E. M. and Mathers, J. C.), pp. 125–138. Cambridge University Press, Cambridge.Google Scholar
Cummings, J. H., Hill, M. J., Bone, E. S., Branch, W. J. and Jenkins, D. J. A. 1979. The effect of meat protein and dietary fibre on colonie function and metabolism. II. Bacterial metabolites in faeces and urine. American Journal of Clinical Nutrition 32: 2094–2101.CrossRefGoogle Scholar
Cummings, J. H. and Macfariane, G. T. 1991. The control and consequences of bacterial fermentation in the human colon. Journal of Applied Bacteriology 70: 443–459.Google Scholar
Drasar, B. S. and Roberts, A. K. 1991. Methods for the study of anaerobic microflora. In Anaerobic microbiology — a practical approach (ed. Levett, P. N.), pp. 183–200. IRL Press, Oxford.CrossRefGoogle Scholar
Edmunds, B. K., Buttery, P. J. and Fisher, C. 1980. Protein and energy metabolism in the growing pig. In Energy metabolism (ed. Mount, L. E.), pp. 129–133. Butterworths, London.Google Scholar
Englyst, H. N., Kingman, S. M. and Cummings, J. H. 1992. Classification and measurement of nutritionally important starch fractions. European Journal of Clinical Nutrition 46: (suppl. 2) S33–S50.Google ScholarPubMed
Filer, L. J. 1988. Modified food starch — an update. Journal of the American Dietetic Association 88: 342–344.Google Scholar
Fry, J. C. 1993. One-way analysis of variance. In Biological data analysis (ed. Fry, J. C.), pp. 3–39. Oxford University Press, Oxford.Google Scholar
Gädeken, D., Schulz, E. and Rohr, K. 1992. [Effect of bacterially fermentable matter on the utilization of metabolizable energy in pigs.] Agribiological Research 45: 321–332.Google Scholar
Gibson, G. R., Cummings, J. H. and Macfarlane, G. T. 1990. Factors affecting hydrogen uptake by bacteria growing in the human large intestine. In Microbiology and biochemistry of strict anaerobes involved in interspecies hydrogen transfer (ed. Bélaich, J.-P., Bruschi, M. and Garcia, J.-L.), FEMS symposium no. 54, pp. 191–202. Plenum Press, New York.Google Scholar
Gibson, S. A. W., Mcfarlan, C, Hay, S. and Macfarlane, G. T. 1989. Significance of microflora in proteolysis in the colon. Applied and Environmental Microbiology 55: 679–683.CrossRefGoogle ScholarPubMed
Gilliland, S.E. and Speck, M. L. 1977. Antagonistic action of Lactobacillus acidophilus toward intestinal and foodborne pathogens in associative culture. Journal of Food Protection 40: 820–823.CrossRefGoogle Scholar
Hansen-Møller, J. 1992. Determination of indolic compounds in pig back fat by solid-phase extraction and gradient high-performance liquid chromatography with special emphasis on the boar taint compound skatole. Journal of Chromatography 624: 479–490.CrossRefGoogle Scholar
Hechard, Y., Dherbomez, M., Cenatiempo, Y. and Letellier, F. 1990. Antagonism of lactic acid bacteria from goats milk against pathogenic strains assessed by the sandwich method. Letters in Applied Microbiology 11: 185–188.CrossRefGoogle Scholar
Hill, M. J., Fadden, K., Fernandez, F. and Roberts, A. K. 1986. Biochemical basis for microbial antagonism in the intestine. In Natural antimicrobial systems (ed. Gould, G. W. Rhodes-Roberts, M. E. Charnley|R., A.K. Cooper, M. and Board, R. G.), FEMS symposium no. 35, pp. 29–39. Bath University Press, Bath.Google Scholar
Hillman, K. and Fox, A. 1994. Effects of porcine faecal lactobacilli on the rate of growth of enterotoxigenic Escherichia coli 0149:K88:K91. Letters in Applied Microbiology 19: 497–500.Google Scholar
Hillman, K., Murdoch, T. A., Spencer, R. J. and Stewart, C. S. 1994. Inhibition of enterotoxigenic Escherichia coli by the microflora of the porcine ileum, in an in vitro semicontinuous culture system. Journal of Applied Bacteriology 76: 294–300.CrossRefGoogle Scholar
Hillman, K., Spencer, R. J., Murdoch, T. A. and Stewart, C. S. 1995. The effect of mixtures of Lactobacillus spp. on the survival of enterotoxigenic Escherichia coli in in vitro continuous culture of porcine intestinal bacteria. Letters in Applied Microbiology 20: 130–133.Google Scholar
Jensen, M. T., Cox, R. P. and Jensen, B. B. 1995. 3-methylindole (skatole) and indole production by mixed populations of pig fecal bacteria. Applied and Environmental Microbiology 61: 3180–3184.CrossRefGoogle ScholarPubMed
Johnsson, E. and Conway, P. L. 1992. Probiotics for pigs. In Probiotics: the scientific basis (ed. Fuller, R.), pp. 260–316. Chapman and Hall, New York.Google Scholar
Kreuzer, M., Kirchgessner, M. and Roth, F.X. 1991. Experimental evaluation of the capacity of the hindgut of sows to ferment purified dietary ingredients and of consequences in nitrogen metabolism. Journal of Animal Physiology and Animal Nutrition S22: 62–66.Google Scholar
McBurney, M. I., Cuff, D. J. and Thompson, L. U. 1990. Rates of fermentation and short-chain fatty acid and gas production of six starches by human faecal microbiota. Journal of the Science of Food and Agriculture 50: 79–88.CrossRefGoogle Scholar
Macfarlane, G. T., Allison, C, Gibson, S. A. W. and Cummings, J. H. 1988. Contribution of the microflora to proteolysis in the human large intestine. Journal of Applied Bacteriology 64: 37–46.CrossRefGoogle ScholarPubMed
Macfarlane, G. T., Hay, S., Macfarlane, S. and Gibson, G.R. 1990. Effect of different carbohydrates on growth, polysaccharidase and glycosidase production by Bacteroides ovatus, in batch and continuous culture. Journal of Applied Bacteriology 68: 179–187.CrossRefGoogle ScholarPubMed
Miles, A. A. and Misra, S. S. 1938. Estimation of the bactericidal power of blood, journal of Hygiene, Cambridge 38: 732–749.Google Scholar
Pluske, J. R., Siba, P. Mv Pethick, D. W., Durmic, Z., Mullan, B.P. and Hampson, D. J. 1996. The incidence of swine-dysentery in pigs can be reduced by feeding diets that limit the amount of fermentable substrate entering the large intestine. Journal of Nutrition 126: 2920–2933.Google ScholarPubMed
Pomeranz, Y. 1992. Research and development regarding enzyme-resistant starch (RS) in the USA: a review. European Journal of Clinical Nutrition 46: (suppl. 2) S63–S68.Google ScholarPubMed
Rabe, E. and Sievert, D. 1992. Effects of baking, pasta production, and extrusion cooking on formation of resistant starch. European Journal of Clinical Nutrition 46: (suppl. 2) S105–S107.Google ScholarPubMed
Reid, C.-A., Hillman, K.. and Henderson, C. 1998. The effect of rétrogradation, pancreatin digestion and amylose/ amylopectin ratio on the fermentation of starch by Clostridium butyricum (NCIMB 7423). Journal of the Science of Food and Agriculture 76: 221–225.Google Scholar
Reid, C.-A., Hillman, K., Henderson, C. and Glass, H. 1996. Fermentation of native and processed starches by the porcine caecal anaerobe Clostridium butyricum (NCIMB 7423). Journal of Applied Bacteriology 80: 191–198.CrossRefGoogle Scholar
Rémésy, C., Demigné, c., and Morand, C. 1995. Metabolism of short-chain fatty acids in the liver. In Physical and clinical aspects of short-chain fatty acids (ed. Cummings, J. H. Rombeau, J. L. and Sakata, T.), pp. 171–190. Cambridge University Press, Cambridge.Google Scholar
Ring, S. G., Gee, J. M., Whittam, M., Orford, P. and Johnson, I. T. 1988. Resistant starch: its chemical form in foodstuffs and effect on digestibility in vitro. Food Chemistry 28: 97–109.Google Scholar
Ryan, B. F., Joiner, B. L. and Ryan, T.A. 1985. Mimtab statistical software, 2nd edition. Minitab, Boston.Google Scholar
Saura-Calixto, F., Goni, L, Bravo, L. and Mañas, E. 1992. Formation of resistant starch in deproteinised and non-deproteinised beans. European Journal of Clinical Nutrition 46:(suppl.2) S109–Slll.Google Scholar
Tannock, G. W. 1992. The lactic microflora of pigs, mice and rats. In The lactic acid bacteria in health and disease, vol. 1 (ed. Wood, B. J. B.), pp. 21–48. Elsevier, London.Google Scholar