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Effect of supplementation with different proportions of barley grain or citrus pulp on the digestive utilization of ammonia-treated straw by sheep

Published online by Cambridge University Press:  18 August 2016

A. Barrios-Urdanetat ,
M. Fondevila  and
C. Castrillo
A. Barrios-Urdanetat*
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
M. Fondevila*
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
C. Castrillo
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
*
†Present address: Facultad de Agronomía, Universidad del Zulia, Apartado 526, Maracaibo, Venezuela.
‡Corresponding author. E-mail: [email protected]
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Abstract

The effect of carbohydrate supplementation on rumen utilization of ammonia-treated straw was studied in four rumen-cannulated ewes (42•0 (s.e. 2•80) kg live weight) in a Latin-square design. Diets were a 50: 50 mixture of ammoniated barley straw and a concentrate made up with different ratios of barley grain and citrus pulp, namely M1 (100: 0), M2 (66: 33), M3 (33: 66) and M4 (0: 100). Concentrates also included soya-bean meal and urea to make diets isonitrogenous. Diets were given in one meal in the morning at 0•8 kg/day. There were no significant differences among treatments in daily weighted means of rumen pH and concentration of volatile fatty acids. Acetate, propionate and butyrate molar proportions were not different among treatments, but proportions of isobutyrate (P < 0•05) and isovalerate (P < 0•10) were higher for M1 and M2. Ammonia concentration immediately before feeding was lower (P < 0•05) for M3 and M4 (72•1 and 51•3 mg/l) than for M1 and M2 (97•0 and 107•9 mg/ l). Total rumen bacterial concentration was higher (P < 0•05) with the highest barley proportion, whereas enzymatic activity against structural carbohydrates of particle-associated bacteria did not differ significantly among treatments. Digestibility of neutral-detergent fibre increased (P < 0•05) with the increase in the proportion of citrus pulp in the concentrate (0•647, 0•674, 0•684 and 0•693 for M1, M2, M3 and M4; s.e. = 0•0086). Urinary excretion of purine derivatives decreased linearly (P < 0•05) as the proportion of citrus pulp in the diet increased, while faecal excretion of purine bases was not significantly affected by dietary treatments.

Keywords

barley strawcitrus pulpmicrobial proteinsrumen digestion.
Type
Ruminant nutrition, behaviour and production
Information
Animal Science , Volume 76 , Issue 2 , April 2003 , pp. 309 - 317
Copyright
Copyright © British Society of Animal Science 2003

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References

Agricultural and Food Research Council. 1993. Energy and protein requirements of ruminants. An advisory manual prepared by the AFRC Technical Committee on Responses to Nutrients. CAB International, Wallingford, UK.Google Scholar
Ashwell, G. 1957. Colorimetric analysis of sugars. In Methods in enzymology (ed. Collowick, S.P. and Kaplan, N.O.), p. 85. Academic Press, NY.Google Scholar
Association of Official Analytical Chemists. 1980. Official methods of analysis, 13th edition. AOAC, Washington, DC.Google Scholar
Bach, A., Yoon, I. K., Stern, M. D., Jung, H. G. and Chester-Jones, H. 1999. Effects of type of carbohydrate supplementation to lush pasture on microbial fermentation in continuous culture. Journal of Dairy Science 82: 153–160.CrossRefGoogle ScholarPubMed
Balcells, J., Fondevila, M., Guada, J. A., Castrillo, C and Surra, J. C. E. 1993. Urinary excretion of purine derivatives and nitrogen in sheep given straw supplemented with different sources of carbohydrates. Animal Production 57: 287–292.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. 1991. Rumen digestion and urinary excretion of purine derivatives in response to urea supplementation of sodium-treated straw fed to sheep. British Journal of Nutrition 69: 721–732.Google Scholar
Balcells, J., Guada, J. A., Peiró, J.M. and Parker, D. S. 1992. Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. Journal of Chromatography 575: 153–157.Google Scholar
Barrios-Urdaneta, A., Fondevila, M., Balcells, J., Dapoza, C. and Castrillo, C. 2000. In vitro microbial digestion of straw cell wall polysaccharides in response to supplementation with different sources of carbohydrates. Australian Journal of Agricultural Research 51: 393–400.Google Scholar
Ben-Ghedalia, D., Yosef, E., Miron, J. and Est, Y. 1989. The effects of starch and pectin rich diets on quantitative aspects of digestion in sheep. Animal Feed Science and Technology 24: 289–298.Google Scholar
Berge, P. and Dulphy, J. P. 1985. Etude des interactions entre forrage et aliment concentre chez le mouton. 1. Facteurs de variation du tax de substitution. Annales de Zootechnie 34: 313–334.Google Scholar
Bryant, M. P. and Burkey, L. A. 1953. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. Journal of Dairy Science 36: 205–217.CrossRefGoogle Scholar
Carey, D. A., Caton, J. S. and Biondini, M. 1993. Influence of energy source on forage intake, digestibility, in situ forage degradation, ruminal fermentation in beef steers fed medium-quality Brome hay. Journal of Animal Science 71: 2260–2269.CrossRefGoogle ScholarPubMed
Castrillo, C., Fondevila, M., Alibes, X. and Joy, M. 1991. Chemical treatments for upgrading lignocellulosic resources and strategies for their utilisation in ruminant feeding. In Production and utilisation of lignocellulosics (ed. Galletti, G. C.), pp. 339–373. Elsevier Applied Science.Google Scholar
Chaney, A. L. and Marbach, E. P. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry 8: 130–132.Google Scholar
Chesson, A. and Monro, J. A. 1982. Legume pectin substances and their degradation in the ovine rumen. Journal of the Science of Food and Agriculture 33: 852–859.Google Scholar
Dehority, B. A., Tirabasso, P. A. and Grifo, A. P. 1989. Most-probable number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium. Applied and Environmental Microbiology 55: 2789–2792.Google Scholar
Demeyer, D. and Nevel, C. van. 1986. Influence of substrate and microbial interactions on efficiency of rumen microbial growth. Reproduction, Nutrition, Development 26: 161–179.CrossRefGoogle Scholar
Dulphy, J. P., Breton, J., Bienaime, A. and Louyot, J. M. 1987. Etude de la valeur alimentaires des pailles de cereales traites ou non a la soude. 1. Influence du traitement a la soude. Annales de Zootechnie 31: 195–214.CrossRefGoogle Scholar
Fahmy, S. T. M., Lee, N. H. and Ørskov, E. R. 1984. Digestion and utilization of straw. 2. Effect of different supplements on the digestion of ammonia-treated straw. Animal Production 38: 75–81.Google Scholar
Flachowsky, G., Koch, H., Tiroke, K. and Matthey, M. 1993. Influence of ratio between wheat straw and ground barley, ground corn or dried sugar beet pulp on in sacco dry matter degradation of ryegrass and wheat straw, rumen fermentation and apparent digestibility in sheep. Archives of Animal Nutrition 43: 157–167.Google Scholar
Fondevila, M., Castrillo, C., Guada, J. A. and Balcells, J. 1994. Effect of ammonia treatment and carbohydrate supplementation of barley straw on rumen liquid characteristics and substrate degradation by sheep. Animal Feed Science and Technology 50: 137–155.CrossRefGoogle Scholar
Harrison, D. G., Beever, D. E., Thompson, D. J. and Osbourn, D. F. 1975. Manipulation of rumen fermentation in sheep by increasing the rate of flow of water from the rumen. Journal of Agricultural Science, Cambridge 85: 93.CrossRefGoogle Scholar
Hatfield, R. D. and Weimer, P. J. 1995. Degradation characteristics of isolated and in situ cell wall lucerne pectic polysaccharides by mixed ruminal microbes. Journal of the Science of Food and Agriculture 69: 185–196.Google Scholar
Hiltner, P. and Dehority, B. A. 1983. Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Applied and Environmental Microbiology 46: 642–648.Google Scholar
Huhtanen, P. 1988. The effects of barley, unmolassed sugar-beet pulp and molasses supplement on organic matter, nitrogen and fibre digestion in the rumen of cattle given a silage diet. Animal Feed Science and Technology 20: 259–278.CrossRefGoogle Scholar
Jouany, J. P. 1982. Volatile fatty acid and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermentor contents. Science des Aliments 2: 131–144.Google Scholar
Kennedy, P. M. and Milligan, L. P. 1978. Transfer of urea from the blood to the rumen of sheep. British Journal of Nutrition 40: 149–154.Google Scholar
McDonald, I.M. 1981. A revised model for the estimation of protein degradability in rumen. Journal of Agriculture Science, Cambridge 96: 251–252.Google Scholar
Martin, C. and Michalet-Doreau, D. 1995. Variation in mass and enzyme activity of rumen microorganisms associated with particles: effect of barley and buffer supplements. Journal of the Science of Food and Agriculture 67: 409–415.CrossRefGoogle Scholar
Mould, F. L. and Ørskov, E.R. 1983. Manipulation of rumen fluid pH and its influence on cellulolysis in sacco, dry matter degradation and the rumen microflora of sheep offered either hay or concentrate. Animal Feed Science and Technology 10: 1–14.CrossRefGoogle Scholar
Schwartz, S.E., Levine, R. A., Weinstock, R. S., Petokas, R. S., Mills, C. A. and Thomas, F. D. 1988. Sustained pectin ingestion: effect on gastric emptying and glucose tolerance in non-insulin dependent diabetic patients. American Journal of Clinical Nutrition 48: 1413.Google Scholar
Silva, A. T., Wallace, R. J. and Ørskov, R. E. 1987. Use of particle-bound microbial enzyme activity to predict the rate and extent of fibre degradation in the rumen. British Journal of Nutrition 57: 407–415.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute. 1988. SAS companion for the Microsoft Windows environment, version 6•12. SAS Institute Inc., Cary, NC.Google Scholar
Strobel, H. J. and Russell, J. B. 1986. Effect of pH and energy spilling on bacterial protein synthesis by carbohydrate-limited cultures of mixed rumen bacteria. Journal of Dairy Science 69: 2941–2947.Google Scholar
Thakur, B. R., Singh, R. K. and Handa, A. K. 1997. Chemistry and uses of pectin (a review). Food Science and Nutrition 37: 47–73.Google Scholar
Titgemeyer, E. C., Bourquin, L. D., Fahey, G.C. Jr., 1992. Disappearance of cell wall monomeric components from fractions chemically isolated from alfalfa leaves and stems following in situ ruminal digestion. Journal of the Science of Food and Agriculture 58: 451–463.Google Scholar
Van Gylswyk, N. O. and Schwartz, H. M. 1984. Microbial ecology of the rumen of animals fed high-fibre diets. In Herbivore nutrition in the subtropics and tropics (ed. F.M.C., Gilchrist and R.I., Mackie), pp. 359–377. The Science Press, Pretoria.Google Scholar
Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583–3597.Google Scholar
Warner, A. C. I. and Stacy, B. D. 1968. The fate of water in the rumen. 2. Water balances throughout the feeding cycle in sheep. British Journal of Nutrition 22: 389–410.Google Scholar