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Contribution of dietary nitrogen and purine bases to the duodenal digesta: comparison of duodenal and polyester-bag measurements

Published online by Cambridge University Press:  02 September 2010

J. F. Pérez ,
J. Balcells ,
J. A. Guada  and
C. Castrillo
J. F. Pérez
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria C/Miguel Servet 177, 50013 Zaragoza, Spain
J. Balcells
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria C/Miguel Servet 177, 50013 Zaragoza, Spain
J. A. Guada
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria C/Miguel Servet 177, 50013 Zaragoza, Spain
C. Castrillo
Affiliation:
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria C/Miguel Servet 177, 50013 Zaragoza, Spain
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Abstract

Four ewes fitted with ruminal and duodenalT-piece cannulas were given fourdietsin a 4 × 4 factorial design. Diets consisted of 700 (HF) or 400 (LF) g/day of ammonia-treated barley straw supplemented respectively with 150 or 600 g/day of concentrate made up with barley plus either soya-bean meal (SBM) or fishmeal (FM) as the protein source, offered at 2-h intervals. Duodenal flowsof digestawere estimated by the dual-phasetechniqueusing CoEDTAand Yb-acetate as markers and (15NH4)2SO4 was infusedinto the rumento label microbial N. Bacteria were isolated from the liquid (LAB) or solid (SAB) rumendigesta. Purinebases (PB) were isolated by precipitationin an acid solution of AgN03, and microbial contribution either to the duodenalnitrogen(N) or PB were determinedby 15N measurements induodenaldigesta and bacteria.Simultaneously, therumen degradation of Nand PB contained in SBM and FM was studiedby incubating supplements in polyesterbags in the rumen.PBcontent (mmol/g dry matter)and guanine: adenine(G/A) ratio of barley strawwas 2·89 and 5·23; barley grain,7·91 and 111;SBM, 18·8 and 1·26; and FM, 58·9 and 6·96, respectively. Duodenal flow ofPB(mmol/day)was significantly higher than PB intake on all diets and G/A ratio showed a meanvalue of 0·97, similarto the ratios determined in SAB(0·80) and LAB (1·04) and muchlower than diets(1·31 to 4·32). Microbial contribution to duodenal Nflow ranged from43·3% to 61·0%, beinghigherin SBM(59·0%)thanin FM(46·7%)diets. However, microbial contribution to duodenal PB was not affected by the experimentaltreatment, accounting for proportionately 0·77 of total PB at the duodenum. Rumen degradability of PB was much higher than that of total N and in both cases degradability was higher in SBM than FM. Direct measurements of non-microbialN were significantly higher than values determined by the polyester-bagmeasurements. However, once corrected forthe endogenousN (52 mgN per kg live weight)contribution, results show edan acceptable agreement. Duodenal flow of PB non-attributable to microbes (unlabelled PB) showed a mean value of 3·25 mmol/daywithouta significanteffect of dietary treatment. However, undegradablePBsupply determinedfor0·02, 0·05 and 0·08 per h fractional out flow rates were proportionately lower than 0·025 with SBM and 0·100 with FM diets of the estimated duodenalPB flow. Despite the magnitudeof the unlabelledduodenalPB, the close agreement between G/A ratios in duodenaldigesta and bacteria suggests thatthe contribution of dietary PB to the duodenalflow was low and seemsto confirm the reliability of values obtained from polyester-bag measurements.

Keywords

digestanitrogenpurinesruminants
Type
Research Article
Information
Animal Science , Volume 65 , Issue 2 , October 1997 , pp. 237 - 245
Copyright
Copyright © British Society of Animal Science 1997

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References

Agricultural and Food Research Council. 1992. Nutritive requirements of ruminant animals: protein. Nutrition Abstracts and Reviews, Series B 62: 787–835.Google Scholar
Aharoni, Y. and Tagari, H. 1991. Use of nitrogen-15 determinations of purinenitrogenfraction of digestato define nitrogen metabolism traits in the rumen. Journal of Dairy Science 74:2540–2547.CrossRefGoogle ScholarPubMed
Balcells, J., Guada, J. A., Castrillo, C. and Gasa, J. 1991. Urinary excretion of allan to in and allantoin precursors by sheep after different rates of purine infusion in to the duodenum. Journal of Agricultural Science, Cambridge 116: 309–317.CrossRefGoogle Scholar
Balcells, J., Guada, J. A., Peiró, J. M. and Parker, D. S. 1992. Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. Journal of Chromatography 575:153–157.CrossRefGoogle ScholarPubMed
Bauchop, T. 1980 Scanning electron microscopy in the study of the microbial digestion of plant fragments in the gut. In Contemporary microbial ecology(ed. Ellwood, J. N., Latham, M. J., Lynch, M. J. and Slater, J. H. ), pp. 305–326. Academic Press, London.Google Scholar
Buresh, R. J., Austin, E. R. and Craswell, E. T. 1982. Analyticalmethodsin15N research. FertilizerResearch 3: 37–62.Google Scholar
Castrillo, C, Lainez, M., Gasa, J. and Guada, J. A. 1992. The effectof increasing the proportion of barley straw in pelleted concentrate diets to lambs on rumen outflow rate and degradation of protein supplements. Animal Production 54: 59–66.Google Scholar
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. and Brown, D. S. 1990. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63: 131–142.CrossRefGoogle ScholarPubMed
Clark, J. H., Klusmeyer, T. H. and Cameron, M. R. 1990. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. Journal of Dairy Science 75: 2304–2323.CrossRefGoogle Scholar
Cotta, H. A. and Hespell, R. D. 1984. Protein and amino acid metabolism of rumen bacteria. In Control of digestion and metabolism in ruminants (ed.Milligan, L. P., Grovum, W. L. and Dobson, A.), pp. 122–136. Prentice Hall, Englewood Cliff, NJ.Google Scholar
Faichney, G. C. 1975. The use of markers to partition digestion within the gastrointestinal tract of ruminants. In Digestion and metabolism in theruminant (ed. MacDonald, I. W. and Warner, A. C. I.), pp. 277–291. Armidale University of New England Publishing Unit.Google Scholar
Fairlamb, A. H. 1989. Novel biochemical pathways in parasitic protozoa. Parasitology 99: (supplement) 93–112.CrossRefGoogle ScholarPubMed
Ganev, G., Ørskov, E. R. and Smart, R. 1979. The effect of roughage or concentrate feeding and rumen retention time on total degradationofproteinintherumen. Journalof Agricultural Science, Cambridge 93:651–656.Google Scholar
Gottlieb, M. 1985. Enzyme regulation in a trypanosomatid: effect ofpurinestarvationonlevelof 3'-nucleotidase activity. Science 227: 72–74.CrossRefGoogle Scholar
Jarrige, R. 1987. Situation and perspectives of the modern protein feedingsystems forruminants. In Feed evaluation and protein requirement systems for ruminants (ed. Jarrige, R. and Alderman, G.), pp. 305–326. CEC.Google Scholar
John, A. and Ulyatt, M. J. 1984. Measurement of protozoa using phosphatidyl choline and of bacteria using nucleic acids in the duodenal digesta of sheep fed chaffed lucerne hay (Medicago sativa L.) diets. Journal of Agricultural Science, Cambridge 102: 33–44.CrossRefGoogle Scholar
Kennedy, P. M., Hazlewood, G. P. and Milligan, L. P. 1984. A comparison of methods for the estimation of the proportion of microbial nitrogen in duodenal digesta, and ofcorrectionformicrobialcontaminationinnylonbags incubated in the rumen of sheep. British Journal of Nutrition 52:403–417.Google Scholar
McAUan, A. B. and Smith, R. H. 1973. Degradation of nucleic acids in the rumen. British Journal of Nutrition 29: 331–345.Google Scholar
Mathers, J. C. and Miller, E. L. 1981. Quantitative studies of food protein degradation and the energetic efficiencyof microbial protein synthesis in the rumen of sheep given chopped lucerne and rolledbarley. British Journal of Nutrition 45:587–604.CrossRefGoogle Scholar
Minato, H. and Suto, T. 1978. Technique forfractionation of bacteria in rumen microbial ecosystem. II. Attachment of bacteria isolated from bovine rumen to cellulose powder in vitro and elution of bacteria attached there from. Journal of General Applied Microbiology 24:1–16.Google Scholar
Mohamed, O. E. and Smith, R. H. 1977 Measurement of protein degradation in the rumen. Proceedings of the Nutrition Society 36: 152A (abstr.).Google ScholarPubMed
Ørskov, E. R. 1982. Protein nutrition in ruminants. Academic Press.Google Scholar
Ørskov, E. R. and McDonald, J. 1979. The estimation of protein degradability in the rumen from adjustedrates of passage. Journal ofAgricultural Science, Cambridge 92: 499–503.CrossRefGoogle Scholar
Ørskov, E. R., Macleod, N. A. and Kyle, D. J. 1986. Flow of nitrogen from the rumen and abomasum in cattle and sheep given protein-free nutrients by intragastric infusion. British Journal of Nutrition 56:241–248.Google Scholar
Pérez, J. F., Balcells, J., Guada, J. A. and Surra, J. C. 1995. Contribution of dietary purine bases to duodenal digesta: effect of forage/concentrate ratio. Animal Science 60: 544 (abstr.).Google Scholar
Pérez, J. F., Rodriguez, C. A., Gonzalez, J., Balcells, J. and Guada, J. A. 1996. Contribution of dietary purine bases to duodenal digesta in sheep.Insitustudiesof purine degradability corrected for microbial contamination. Animal Feed Science and Technology 62:251–262.Google Scholar
Schelling, G. T., Koening, S. E. and Jackson, T. C. Jr. 1982. Nucleic acids and purine or pirimidine bases as markers for protein synthesis in the rumen. In Protein requirements for cattle (ed. Owens, F. N.), pp. 1–9. Oklahoma University.Google Scholar
Siddons, R. C., Paradine, J., Beever, D. E. and Cornell, P.R. 1985a. Ytterbium acetateasa particulate-phase digesta-flow marker. British Journal of Nutrition 54: 509–519.Google Scholar
Siddons, R. C, Paradine, J., Gale, D. L. and Evans, R. T. 1985b. Estimation of the degradability of dietary protein in the sheep rumen by in vivo and in vitro procedures. British Journal of Nutrition 54:545–561.CrossRefGoogle ScholarPubMed
Smith, R. H. and McAllan, A. B. 1970. Nucleic acid metabolism in the ruminant. 2. Formation of microbial nucleic acids in the rumen in relation to the digestion of food nitrogen, and the fate of dietary nucleic acids. British Journal of Nutrition 24: 545–556.Google Scholar
Smith, R. H., McAllan, A. B., Hewitt, D. and Lewis, P. E. 1978. Estimation of amounts of microbial and dietary nitrogen compounds entering the duodenum of cattle. Journal of Agricultural Science, Cambridge 90:557–568.Google Scholar
Steel, R. G. D. and Torrie, J. H. 1960. Principlesand proceduresof statistics. McGraw-HillBook Company Inc., London.Google Scholar
Sutton, J. D., Smith, R. H., McAllan, A. B., Storry, J. E. and Corse, D. A. 1975. Effectsof variations in dietary protein and supplements of cod-liver oil on energy digestion and microbial synthesis in the rumen of sheep fed hayand concentrates. Journal of AgriculturalScience, Cambridge 84: 317–326.CrossRefGoogle Scholar
Zelaya, J. C. 1994. Degradatión ruminal de las bases púricasdeorigen alimentario. Tesis-Master of Science. Instituto Agronómico Mediterraneo de Zaragoza.Google Scholar
Zinn, R. A. and Owens, F. N. 1982. Rapid procedure for quantifying nucleic acid content of digesta. In Protein requirements forcattle, symposium (ed. Owens, F. N.), pp. 26–30. Oklahoma State University, Stillwater.Google Scholar