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Comparison of calorimetry and the doubly labelled water technique for the measurement of energy expenditure in Equidae

Published online by Cambridge University Press:  18 August 2016

Z. Fuller ,
C. A. Maltin ,
E. Milne ,
G.S. Mollison ,
J. E. Cox  and
C. McG. Argo
Z. Fuller
Affiliation:
School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK
C. A. Maltin
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
E. Milne
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
G.S. Mollison
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
J. E. Cox
Affiliation:
Department of Veterinary Clinical Sciences and Animal Husbandry, University of Liverpool, Leahurst, Neston CH64 7TE, UK
C. McG. Argo
Affiliation:
School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, UK
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Abstract

Evaluations of the energy requirements of working animals have been confounded by the constraints of indirect calorimetric techniques (Cal). This study sought to investigate a non-restrictive methodology, the doubly labelled water (DLW; 2H218O) technique, for the measurement of energy expenditure in free ranging equids. Six pony geldings were intravenously injected with DLW in two sequential studies that permitted first, isotope equilibration and half-lives to be determined and then second, heat production (HP) to be measured simultaneously by DLW and traditional (Cal) techniques.

In study 1, three animals were injected with DLW, three animals were untreated controls. Blood samples were collected every 30 min for 12 h and thereafter at 24 h intervals for 14 days. Isotopes equilibrated throughout the body water pool within 300 (2H) and 240 (18O) min and half-lives were 6.3 ± 0.6 days (2H) and 5.6 ± 0.4 days (18O).

In study 2, HP was simultaneously determined by Cal and DLW over a 4-day period. Animals (no. = 6) were assigned to pairs and in successive weeks two ponies were injected with DLW and confined to metabolism chambers 12 h later. Cal HP was 0.51 ± 0.02 MJ/kg M0.75 per day compared with 0.48 ± 0.29 MJ/kg M0.75 per day estimated by DLW. Maintenance metabolizable energy intake was 0.53 ± 0.01 MJ/kg M0.75 per day (Cal) and 0.50 ± 0.01 MJ/kg M0.75 per day (DLW). Validation of the DLW technique may empower essential, systematic appraisal of energy requirements in unrestrained working horses.

Keywords

deuteriumhorsesmetabolismnutritionoxygen-18
Type
Non-ruminant nutrition, behaviour and production
Information
Animal Science , Volume 78 , Issue 2 , April 2004 , pp. 293 - 303
Copyright
Copyright © British Society of Animal Science 2004

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References

Blaxter, K. L. 1962. Fasting metabolism in sheep. British Journal of Nutrition 16: 615–626.CrossRefGoogle Scholar
Blaxter, K. L., Brockway, J. M. and Boyne, A. W. 1972. A new method for estimating the heat production of animals. Quarterly Journal of Experimental Physiology 57: 60–72.Google Scholar
Boyd, I. L., Woakes, A. J., Butler, P. J., Davis, R. W. and Williams, T. M. 1995. Validation of heart-rate and doubly labelled water as measures of metabolic-rate during swimming in California sea lions. Functional Ecology 9: 151–160.Google Scholar
Brouwer, E. 1965. Report of the sub-committee on constants and factors. In Energy metabolism (ed. Blaxter, K. L.), European Association for Animal Production publication no. 11, pp. 441–443. Academic Press, London.Google Scholar
Burke, D. J. and Albert, W. W. 1978. Methods for measuring physical condition and energy expenditure in horses. Journal of Animal Science 46: 1666–1672.Google Scholar
Coward, W. A., Prentice, A. M., Murgatroyd, P. R., Davies, H. L., Cole, T. J., Sawyer, M., Goldberg, G. R., Halliday, D. A. and MacNamara, J. P. 1985. Measurement of CO2 and water production rates in man using 2H218O-labelled H2O; comparison between calorimeter and isotope values. In Human energy metabolism, European nutrition report no. 5 (ed. A. J. H. van Es), pp. 126–128. CIP-gegevens koninkijke, Den Haag.Google Scholar
Cuddeford, D., Pearson, R. A., Archibald, R. F. and Muirhead, R. H. 1995. Digestibility and gastro-intestinal transit time of diets containing different proportions of alfalfa and oat straw given to thoroughbreds, Shetland ponies, Highland ponies and donkeys. Animal Science 61: 407–417.Google Scholar
Cymbaluk, N. F. and Christison, G. I. 1990. Environmental effects on thermoregulation and nutrition of horses. Veterinary Clinics of North America, Equine Practice 6: 355–372.CrossRefGoogle ScholarPubMed
Cymbaluk, N. F., Christison, G. I. and Leach, D. H. 1989. Nutrient utilization by limit- and ad libitum-fed growing horses. Journal of Animal Science 67: 414–425.Google Scholar
Fancy, S. G., Blanchard, J. M., Holleman, D. F., Kokjer, K. J. and White, R. G. 1986. Validation of the doubly labelled water method using a ruminant. American Journal of Physiology 251: R143-R149.Google Scholar
Fuller, Z., Cox, J. E. and Argo, C.McG. 2001. Photoperiodic entrainment of seasonal changes in the appetite, feeding behaviour, growth rate and pelage of pony colts. Animal Science 72: 65–74.Google Scholar
Fuller, Z., Maltin, C. A., Mollison, G. S., Cox, J. E. and Argo, C.McG. 1999. Validation of the doubly labelled water technique to accurately predict the daily energy requirements of horses. Proceedings of the 16th equine nutrition and physiology symposium, Raleigh, USA, pp. 18-19 (abstr.).Google Scholar
Gonfiantini, R. 1978. Standard for stable isotope measurements in natural compounds. Nature 271: 534–536.Google Scholar
Haggarty, P. 1991. Potential of the doubly labelled water method for estimating heat production in farm animals. Journal of Agricultural Science, Cambridge 117: 141–148.Google Scholar
Hintz, H. F. 1990. Digestion in ponies and horses. Equine Practice 12: 5–6.Google Scholar
Jones, J. H. and Carlson, G. P. 1995. Estimation of metabolic energy cost and heat production during a three-day event. Equine Veterinary Journal 20: (suppl.) 23–30.Google Scholar
Judson, G. L. and Mooney, G. J. 1983. Body water and water turnover rate in thoroughbred horses in training. In Equine exercise physiology (ed. D. H., Snow, S. G. B., Persson and Rose, R. J.), pp. 371–386. Granta Editions, Cambridge.Google Scholar
Kane, E., Baker, J. P. and Bull, L. S. 1979. Utilisation of a corn oil supplemented diet by the pony. Journal of Animal Science 48: 1379–1384.Google Scholar
Kane, R. A., Fisher, M., Parrett, D. and Lawrence, L. M. 1987. Estimating fatness in horses. Proceedings of the 10th equine nutrition and physiology symposium, Fort Collins, CO, pp. 127–131.Google Scholar
Kearns, C. F., McKeever, K. H., Malinowski, K., Struck, M. B. and Abe, T. 2001. Chronic administration of therapeutic levels of clenbuterol acts as a repartitioning agent. Journal of Applied Physiology 91: 2064–2070.Google Scholar
King, J. M., Kingaby, G. P., Colvin, J. G. and Heath, B. R. 1975. Seasonal variation in water turnover by oryx and eland on the Galana Game Research Project. East African Wildlife Journal 13: 287–296.Google Scholar
Kleiber, M. 1932. Body size and metabolism. Hilgardia 6: 315–353.Google Scholar
Lawrence, L. M. 1994. Nutrition and the athletic horse. In The athletic horse (ed. Hodgson, D. R. and Rose, R. J.), pp. 205–230. W. B. Saunders Company, Philadelphia.Google Scholar
Lifson, N., Gordon, G. B. and McClintock, R. 1955. Measurement of total carbon dioxide production by means of D2 18O. Journal of Applied Physiology 7: 704–710.Google Scholar
Lifson, N. and McClintock, R. 1966. Theory of the use of turnover rates of body water for measuring energy and material balance. Journal of Theoretical Biology 12: 46–74.Google Scholar
McBride, G. E., Christopherson, R. J. and Sauer, W. 1985. Metabolic rate and plasma thyroid hormone concentrations of mature horses in response to changes in ambient temperature. Canadian Journal of Animal Science 65: 375–382.Google Scholar
McCann, J. S., Meacham, T. N. and Fontenot, J. P. 1987. Energy utilisation and blood traits of ponies fed fat-supplemented diets. Journal of Animal Science 65: 1019–1026.Google Scholar
MacCormack, J. A. D. and Bruce, J. M. 1991. The horse in winter—shelter and feeding. Farm Building Progress 105: 10–13.Google Scholar
Martin-Rosset, W. and Vermorel, M. 1991. Maintenance energy requirement variations determined by indirect calorimetry and feeding trials in light horses. Proceedings of the 11th symposium of the Equine Nutrition and Physiology Society, Equine Veterinary Science Stillwater, USA, pp. 42–45.Google Scholar
Midwood, A. J. 1990. Application of the doubly labelled water method for measuring CO2 production to sheep. Ph.D. thesis, University of Aberdeen.Google Scholar
Midwood, A. J., Haggerty, P., McGaw, B. A., Mollison, G. S., Milne, E. and Duncan, G. J. 1994. Validation in sheep of the doubly-labelled water method for estimating CO2 production. American Journal of Physiology 266: (Regulatory, Integrative and Comparative Physiology 35) R169-R179.Google Scholar
Midwood, A. J., Haggarty, P., McGaw, B. A. and Robinson, J. J. 1989. Methane production in ruminants: its effect on the doubly-labelled water method. American Journal of Physiology 257: (Regulatory, Integrative and Comparative Physiology 26) R1488-R1495.Google Scholar
Midwood, A. J., Haggarty, P., Milne, E. and McGaw, B. A. 1992. Factors affecting the analysis of 18O-enriched aqueous samples when using CO2 equilibration in Vacutainers. Applied Radioactive Isotopes 43: 1341–1347.Google Scholar
Nagy, K. A. 1980. CO2 production in animals: analysis of the potential errors in the doubly labelled water technique. American Journal of Physiology 238: (Regulatory, Integrative and Comparative Physiology 7) R466-R473.Google Scholar
Pagan, J. D. and Hintz, H. F. 1986. Equine energetics. I. Relationship between body weight and energy requirements in horses. Journal of Animal Science 63: 815–821.Google Scholar
Robb, J., Harper, R. B., Hintz, H. F., Reid, J. T., Lowe, J. E., Schryver, H. F. and Rhee, M. S. S. 1972. Chemical composition and energy value of the body, fatty acid composition of adipose tissue and liver and kidney size in the horse. Animal Production 14: 25–34.Google Scholar
Schoeller, D. A. and Webb, P. 1984. Five-day comparison of the doubly labelled water method with respiratory gas exchange. American Journal of Clinical Nutrition 40: 153–158.Google Scholar
Slade, L. M. and Hintz, H. F. 1969. Comparison of digestion in horses, ponies, rabbits and guinea pigs. Journal of Animal Science 28: 842–843.CrossRefGoogle ScholarPubMed
Smith, B. S. W. and Sykes, A. R. 1974. The effect of route of dosing and method of estimation of tritiated water space on the determination of total body water and the prediction of body fat in sheep. Journal of Agricultural Science, Cambridge 82: 105–112.Google Scholar
Sneddon, J. C., Walt, J. G. van der and Mitchell, G. 1991. Water homeostasis in desert-dwelling horses. Journal of Applied Physiology 71: 112–117.Google Scholar
Sneddon, J. C., Walt, J. G. van der and Mitchell, G. 1993. Effect of dehydration on the volumes of body fluid compartments in horses. Journal of Arid Environments 24: 397–408.Google Scholar
Speakman, J. R. 1997. Doubly-labelled water: theory and practice. Chapman and Hall, London.Google Scholar
Speakman, J. R., Perez-Camargo, G., McCappin, T., Frankel, T., Thomson, P. and Legrand-Defretin, V. 2001. Validation of the doubly-labelled water technique in the domestic dog (Canis familiaris). British Journal of Nutrition 85: 75–87.Google Scholar
Springell, P. H. 1968. Water content and water turnover in beef cattle. Australian Journal of Agricultural Research 19: 129–144.Google Scholar
Vermorel, M., Martin-Rosset, W. and Vernet, J. 1997a. Energy utilization of twelve forages or mixed diets for maintenance by sport horses. Livestock Production Science 47: 157–167.Google Scholar
Vermorel, M., Vernet, J. and Martin-Rosset, W. 1989. Energy utilisation and diurnal variations of energy expenditure in saddle horses fed near maintenance. In Energy metabolism of farm animals. Proceedings of the 11th symposium of the European Association for Animal Production, Lanteren, Netherlands, EAAP publication no. 43, pp. 267–270.Google Scholar
Vermorel, M., Vernet, J. and Martin-Rosset, W. 1997b. Digestive and energy utilisation of two diets by ponies and horses. Livestock Production Science 51: 13–19.Google Scholar
Westervelt, R. G., Stouffer, J. R., Hintz, H. F. and Schryver, H. F. 1976. Estimating fatness in horses and ponies. Journal of Animal Science 43: 781–785.Google Scholar
Wong, W. W., Lee, L. S. and Klein, P. D. 1987. Deuterium and oxygen-18 measurement on microlitre samples of urine, plasma, saliva and human milk. American Journal of Clinical Nutrition 45: 905–913.Google Scholar
Wooden, G. R., Knox, K. L. and Wild, C. L. 1970. Energy metabolism in light horses. Journal of Animal Science 30: 544–548.Google Scholar