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The behavioural, endocrine and immune responses of sheep to isolation

Published online by Cambridge University Press:  02 September 2010

M. S. Cockram ,
M. Ranson ,
P. Imlah ,
P. J. Goddard ,
C. Burrells  and
G. D. Harkiss
M. S. Cockram
Affiliation:
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Veterinary Field Station, Easter Bush, Roslin, Midlothian EH25 9RG
M. Ranson
Affiliation:
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Veterinary Field Station, Easter Bush, Roslin, Midlothian EH25 9RG
P. Imlah
Affiliation:
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Veterinary Field Station, Easter Bush, Roslin, Midlothian EH25 9RG
P. J. Goddard
Affiliation:
Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB9 2QJ
C. Burrells
Affiliation:
Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH
G. D. Harkiss
Affiliation:
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Summerhall, Edinburgh EH9 1QH
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Abstract

Two groups of five sheep (7 months of age) were moved and isolated in pens which did not allow visual and tactile contact with other sheep for 24 h. They were then moved back to their original pens where visual and tactile contact was possible. After 24 h the procedure was repeated seven times for one group (group 8-1) and thirteen times for the other group (group 14-1). One group (control) of five sheep remained in pens where visual and tactile contact was possible. When isolated the lambs spent more time standing still in an alert posture, less time eating and resting, and vocalized more than control lambs. The heart rate of the lambs increased when they were moved between pens and during isolation. The plasma concentration of cortisol was significantly increased after 1·5 h and 3 h of isolation. The plasma concentration of prolactin was increased after 1·5 h of isolation. After 3 h of isolation the number of neutrophils in the blood was increased and the numbers of T-lymphocytes (CD2), T-helper-lymphocytes (CD4) and yd-lymphocytes (T19) were decreased. After 24 h of isolation the lymphocyte blastogenic response to Con A was lower and the numbers of T-lymphocytes and T-helper-lymphocytes were still less than those in control lambs. Although there were still behavioural changes when the lambs were isolated for the seventh time, no cortisol, prolactin and leucocyte changes were found. These results suggest that stressors similar to isolation, which can occur during normal management practice, may elicit short-term leucocyte changes in lambs.

Keywords

behaviourimmune competenceleucocytessheepstress
Type
Research Article
Information
Animal Production , Volume 58 , Issue 3 , June 1994 , pp. 389 - 399
Copyright
Copyright © British Society of Animal Science 1994

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References

Baldock, N. M. and Sibly, R. M. 1990. Effects of handling and transportation on the heart rate and behaviour of sheep. Applied Animal Behaviour Science 28:15–39.Google Scholar
Brooks, A. N. and White, A. 1990. Activation of pituitary-adrenal function in fetal sheep by corticotropin-releasing factor and arginine vasopressin. journal of Endorcinology 124:27–35.Google ScholarPubMed
Burrells, C. and Wells, P. W. 1977. In vitro stimulation of ovine lymphocytes by various mitogens. Research in Veterinary Science 23:84–87.Google Scholar
Carlson, G. P. and Kaneko, J. J. 1976. Influence of prednisolone on intravascular and granulocyte kinetics of calves under nonsteady state conditions. American Journal of Veterinary Research 37:149–151.Google ScholarPubMed
Cockram, M. S., Imlah, P., Goddard, P. J., Harkiss, G. D. and Waran, N. K. 1993. The behavioural, endocrine and leucocyte response of ewes to repeated removal of lambs before the age of natural weaning. Applied Animal Behaviour Science 38:127–142.Google Scholar
Collins, M. T. and Suarez-Guemes, F. 1985. Effect of hydrocortisone on circulating lymphocyte numbers and mitogen-induced blastogenesis in lambs. American journal of Veterinary Research 46:836–840.Google ScholarPubMed
Coppinger, T. R., Minton, J. E., Reddy, P. G. and Blecha, F. 1991. Repeated restraint and isolation stress in lambs increases pituitary-adrenal secretions and reduces cell-mediated immunity. Journal of Animal Science 69:2808–2814.Google Scholar
Davis, S. L., Ohlson, D. L., Klindt, J. and Everson, D. O. 1979. Estimates of repeatability in the temporal patterns of secretion of growth hormone (GH), prolactin (PRL) and thyrotrophin (TSH) in sheep, journal of Animal Science 49:724–728.Google Scholar
Ebling, F. J. P. and Lincoln, G. A. 1987. β-endorphin secretion in rams related to season and photoperiod. Endocrinology 120:809–818.Google Scholar
Fordham, D. P., Lincoln, G. A., Ssewannyana, E. and Rodway, R. G. 1989. Plasma β-endorphin and cortisol concentrations in lambs after handling, transport and slaughter. Animal Production 49:103–107.Google Scholar
Griffin, J. F. T. 1989. Stress and immunity: a unifying concept. Veterinary Immunology and Immunopathology 20:263–312.Google Scholar
Jephcott, E. H., McMillen, I. C., Rushen, J. P. and Thorburn, G. D. 1987. A comparison of the effects of electroimmobilisation and, or, shearing procedures on ovine plasma concentrations of β-endorphin/β-lipoprotein and cortisol. Research in Veterinary Science 43:97–100.Google Scholar
Mackay, C. 1988. Sheep leukocyte molecules: a review of their distribution, structure and possible function. Veterinary Immunology and Immunopathology 19:1–20.CrossRefGoogle ScholarPubMed
McGillen, J. and Phair, J. 1979. Polymorphonuclear leukocyte adherence to nylon: effect of oral corticosteroids. Infection and Immunity 26:542–546.Google Scholar
McNeilly, A. S. and Andrews, P. 1974. Purification and characterization of caprine prolactin. journal of Endocrinology 60:359–367.Google Scholar
Markham, B., Stashak, P. W., Prescott, B., Amsbaugh, D. F. and Baker, P. J. 1978. Selective sensitivity to hydrocortisone of regulatory functions that determine the magnitude of the antibody response to type III pneumococcal polysaccharide. journal of Immunology 121:829–834.CrossRefGoogle ScholarPubMed
Minton, J. E. and Blecha, F. 1990. Effect of acute stressors on endocrinological and immunological functions in lambs. Journal of Animal Science 68:3145–3151.Google Scholar
Moberg, G. P. 1985. Biological response to stress: key to assessment of well-being. In Animal stress (ed. Moberg, G. P.), pp. 245–267. American Physiological Society, Bethesda.Google Scholar
Moberg, G. P., Anderson, C. O. and Underwood, T. R. 1980. Ontogeny of adrenal and behavioural responses of lambs to emotional stress, journal of Animal Science 51:138–142.Google Scholar
Morris, B., Pedersen, N. C. and Trevella, W. 1986. The lymphoid apparatus of the sheep and the recirculation of lymphocytes. In The ruminant immune system in health and disease (ed. Morrison, W. I.), pp. 204–219. Cambridge University Press, Cambridge.Google Scholar
Parrott, R. F., Thornton, S. N., Forsling, M. L. and Delaney, C. E. 1987. Endocrine and behavioural factors affecting water balance in sheep subjected to isolation stress. Journal of Endocrinology 112:305–310.Google Scholar
Parrott, R. F., Thornton, S. N. and Robinson, J. E. 1988. Endocrine responses to acute stress in castrated rams: no increase in oxytocin but evidence for an inverse relationship between cortisol and vasopressin. Ada Endorcinologica (Copenhagen) 117:381–386.Google ScholarPubMed
Price, E. O. and Thos, J. 1980. Behavioural responses to short-term social isolation in sheep and goats. Applied Animal Ethology 6:331–339.Google Scholar
Shannon, A. D. and Jones, M. A. S. 1974. Influence of corticotrophic and corticosteroid hormones on the output of cells from the popliteal lymph node of the sheep. Australian Journal of Experimental Biology and Medical Science 52:515–525.Google Scholar
Wells, P. W., Evans, H. B., Burrells, C., Sharp, J. M., Gilmour, N. J. L., Thompson, D. A. and Rushton, B. 1979. Inability of passively acquired antibody to protect lambs against experimental pasteurellosis. Infection and Immunity 26:25–59.Google Scholar