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An evaluation of the success of MOET in two breeds of hill sheep maintained under normal systems of hill flock management

Published online by Cambridge University Press:  18 August 2016

F. Bari ,
M. Khalid ,
W. Haresign ,
B. Merrell ,
A. Murray  and
R.I.W. Richards
F. Bari
Affiliation:
Animal Sciences Group, Welsh Institute of Rural Studies, Llanbadarn Campus, University of Wales, Aberystwyth SY23 3AL
M. Khalid
Affiliation:
Animal Sciences Group, Welsh Institute of Rural Studies, Llanbadarn Campus, University of Wales, Aberystwyth SY23 3AL
W. Haresign
Affiliation:
Animal Sciences Group, Welsh Institute of Rural Studies, Llanbadarn Campus, University of Wales, Aberystwyth SY23 3AL
B. Merrell
Affiliation:
ADAS Redesdale, Rochester, Otterburn, Northumberland NE19 1SB
A. Murray
Affiliation:
ADAS Redesdale, Rochester, Otterburn, Northumberland NE19 1SB
R.I.W. Richards
Affiliation:
ADAS Pwllpeiran, Cwmystwyth, Aberystwyth SY23 4AB
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Abstract

This study was undertaken to investigate factors affecting the success of multiple ovulation and embryo transfer (MOET) in Scottish Blackface (no. = 120) and Welsh Mountain (no. = 120) ewes, over a period of 2 years using a laparoscopic procedure for both embryo recovery and transfer. Superovulation was induced with ovine FSH, with 98 to 100% of ewes of both the breeds responding to the treatment. The overall mean superovulatory responses were 15⋅0 (s.e. 0⋅8) and 12⋅5 (s.e. 0⋅7) for Scottish Blackface and 15⋅3 (s.e. 0⋅9) and 12·8 (s.e. 0⋅8) for Welsh Mountain ewes in years 1 and 2, respectively. However, there was a wide degree of variation in superovulatory responses within each breed, with a range of 3 to 29 in Scottish Blackface and 1 to 40 in Welsh Mountain ewes. The mean embryo recovery rate was 71⋅9 (s.e. 3⋅5) % and 69⋅6 (s.e. 3⋅4) % for Scottish Blackface and 57⋅5 (s.e. 4⋅1) % and 60⋅6 (s.e. 3⋅6) % for Welsh Mountain ewes in years 1 and 2, respectively. The mean number of embryos recovered from Welsh Mountain ewes was significantly (P < 0⋅05) lower than that from Scottish Blackface ewes in both years. The lower mean number of embryos recovered in year 2 for both breeds was entirely a reflexion of the lower superovulatory responses in year 2. A significant (P < 0⋅001) relationship was observed between superovulatory response and the number of embryos recovered for both breeds. Some 77% and 72% of Scottish Blackface ewes and 65% and 73% of Welsh Mountain ewes yielded four or more transferable embryos in years 1 and 2, respectively. Neither the mean number nor the mean percentage of transferable embryos per donor ewe differed between breeds or years. A significant (P < 0⋅001) negative relationship was observed between the time of onset of oestrus and both superovulatory response and number of embryos recovered in Scottish Blackface ewes only. Embryo quality was affected by the time of onset of oestrus. In both breeds, the highest proportion of grade 1+2 embryos and the lowest proportion of unfertilized/degenerate embryos occurred in the middle range time, with a reduction in the proportion of grade 1+2 embryos in ewes that came into oestrus either early <19 h) or late (>30 h) after sponge removal. Only one embryo was transferred to each recipient and the embryo survival rates were 76⋅8% and 74⋅6% (Scottish Blackface), and 69⋅6% and 87⋅3% (Welsh Mountain) for years 1 and 2, respectively. Overall the results of this study suggest that MOET is as successful in hill ewes as has been reported for lowland breeds, even without making any major concessions to their hill status.

Keywords

breedingMOETsheep
Type
Research Article
Information
Animal Science , Volume 69 , Issue 2 , October 1999 , pp. 367 - 376
Copyright
Copyright © British Society of Animal Science 1999

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References

Armstrong, D.T. and Evans, G. 1983. Factors influencing success of embryo transfer in sheep and goats. Theriogenology 19: 31–42.CrossRefGoogle Scholar
Betteridge, K.J. and Moore, N.W. 1977. Superovulation techniques and results in cattle. In Embryo transfer in farm animals: a review of techniques and applications. Canada Department of Agriculture monograph no. 16.Google Scholar
Boland, M.P., Kelly, P., Crosby, T.F. and Roche, J.F. 1995. The effect of type and number of FSH injections on superovulatory response in ewes. Animal Science 60: 524–525 (abstr.).Google Scholar
Boland, M.P., O’Doherty, J.V. and Crosby, T.F. 1993. Superovulation in sheep influenced by FSH and body condition. Proceedings of the ninth meeting of the European Embryo Transfer Association (Lyon), p. 158.Google Scholar
Bouters, R., Moyaert, I., Corijn, M. and Vandeplassche, M. 1983. The use of PMSG antiserum in superovulated cattle: endocrinological changes and effects of timing on ovulation. Zuchthygiene 18: 172–177.CrossRefGoogle Scholar
Campbell, J.W., McDonald, M.F. and Wickham, G.A. 1994. Hormonal and ovarian responses in Romney ewe hoggets after synchronisation and superovulation treatment. Proceedings of the New Zealand Society of Animal Production 54: 239–242.Google Scholar
Cognie, Y., Chupin, D. and Saumande, J. 1986. The effect of modifying FSH/LH ratio during the superovulatory treatment in ewes. Theriogenology 25: 148.CrossRefGoogle Scholar
Dingwall, W.S., Fernie, K., Fitzsimons, J. and McKelvey, W.A.C. 1991. MOET in Suffolks: increasing the superovulation rate. Animal Production 52: 612–613 (abstr.).Google Scholar
Dingwall, W.S., McKelvey, W.A.C., Mylne, J. and Simm, G. 1993. An evaluation of MOET in Suffolk sheep. Animal Production 56: 444 (abstr.).Google Scholar
Fernie, K., Dingwall, W.S., McKelvey, W.A.C. and Fitzsimons, J. 1993. Superovulation in the ewe: the effects of source of gonadotropin, season, breed and age. Animal Production 56: 433 (abstr.).Google Scholar
Jabbour, H.N. and Evans, G. 1991. Superovulation of Merino ewes with an ovine pituitary follicle stimulating hormone extract. Reproduction, Fertility and Development 3: 561–569.CrossRefGoogle ScholarPubMed
Joyce, I.M., Khalid, M. and Haresign, W. 1998. Growth hormone priming as an adjunct treatment in superovulatory protocols in the ewe alters follicle development but has no effect on ovulation rate. Theriogenology 50: 873–884.CrossRefGoogle ScholarPubMed
McEvoy, T.G., Robinson, J.J., Aitken, R.P., Findlay, P.A., Palmer, R.M. and Robertson, I.S. 1995. Dietary-induced suppression of preovulatory progesterone concentrations in superovulated ewes impairs the subsequent in vivo and in vitro development of their ova. Animal Reproduction Science 39: 89–107.CrossRefGoogle Scholar
McEvoy, T.G., Robinson, J.J., Aitken, R.P., Kyle, C.E. and Robertson, I.S. 1993. The effect of feeding level during a 12-day progesterone-priming period on the viability of embryos collected from superovulated ewes. Animal Production 56: 432 (abstr.).Google Scholar
McKelvey, W.A.C. and Robinson, J.J. 1984. Normal lambs born following transfer of embryos by laparoscopy. Veterinary Record 115: 230.CrossRefGoogle ScholarPubMed
McKelvey, W.A.C., Robinson, J.J., Aitken, R.P. and Robertson, I.S. 1986. Repeated recoveries of embryos from ewes by laparoscopy. Theriogenology 25: 855–865.CrossRefGoogle Scholar
McKelvey, W.A.C. and Simm, G. 1995. Intrauterine insemination, transcervical insemination and embryo transfer for practical breed improvement in small ruminants. Proceedings of the 46th annual meeting of the European Association for Animal Production, Prague, pp. 1–12.Google Scholar
McMillan, W.H. and Hall, D.R.H. 1991. Superovulation in ewes: are follicle numbers a useful predictor of ovulation rate? Proceedings of the New Zealand Society of Animal Production 51: 133–137.Google Scholar
McMillan, W.H. and Hall, D.R.H. 1994. Laparoscopie transfer of ovine and cervine embryos using the transpic technique. Theriogenology 42: 137–146.CrossRefGoogle Scholar
Martemucci, G., D’Alessandro, A., Toteda, F., Facciolongo, A.M. and Gambacorta, M. 1995. Embryo production and endocrine responses in ewes superovulated with PMSG, with or without monoclonal anti-PMSG administration at different times. Theriogenology 44: 691–703.CrossRefGoogle ScholarPubMed
Monget, P. and Monniaux, D. 1995. Growth factors and the control of folliculogenesis. Journal of Reproduction and Fertility, Supplement 49: 321–333.Google ScholarPubMed
Mutiga, E.R. and Baker, A.A. 1984. Transfer of sheep embryos through a laparoscope. Veterinary Record 114: 401–402.CrossRefGoogle ScholarPubMed
Picazo, R.A., Cocero, M.J., Barragan, M. and Sebastian, A.L. 1996. Effects of LH administration at the end of an FSH superovulatory regimen on ovulation rate and embryo production in three breeds of sheep. Theriogenology 45: 1065–1073.CrossRefGoogle ScholarPubMed
Rainio, V. 1991. PMSG — dose on Finn sheep embryo production. Theriogenology 35: 261.CrossRefGoogle Scholar
Robinson, J.J., Wallace, J.M. and Aitken, R.P. 1989. Fertilization and ovum recovery rates in superovulated ewes following cervical insemination or laparoscopie intrauterine insemination at different times after progestagen withdrawal and in one or both uterine horns Journal of Reproduction and Fertility 87: 771–782.CrossRefGoogle ScholarPubMed
Ryan, J.P., Hunton, J.R. and Maxwell, W.M.C. 1991. Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotrophin and follicle stimulating hormone. Reproduction, Fertility and Development 3: 551–560.CrossRefGoogle ScholarPubMed
Samartzi, F., Belibasaki, S., Vainas, E. and Boscos, C. 1995. Plasma progesterone concentration in relation to ovulation rate and embryo yield in Chios ewes superovulated with PMSG. Animal Reproduction Science 39: 11–21.CrossRefGoogle Scholar
Scudamore, C.L., McEvoy, T.G., Aitken, R.P., Robinson, J.J. and Robertson, I.S. 1993a. The effect of two different levels of progesterone priming on the response of ewes to superovulation. Theriogenology 39: 433–442.CrossRefGoogle ScholarPubMed
Scudamore, C.L., Robinson, J.J. and Aitken, R.P. 1991. The effect of timing of laparoscopic insemination in superovulated ewes, with or without sedation, on the recovery of embryos, their stage of development and subsequent viability. Theriogenology 35: 907–914.CrossRefGoogle ScholarPubMed
Scudamore, C.L., Robinson, J.J., Aitken, R.P. and Robertson, I.S. 1993b. The effect of method of oestrus synchronisation on the response of ewes to superovulation with porcine follicle stimulating hormone. Animal Reproduction Science 34: 127–133.CrossRefGoogle Scholar
Stefani, J.S., Palho, M.D.C., Christmann, L., Rosa, J.M., Silveria, M.C. and Rodrigues, J.L. 1990. Laparoscopic versus surgical transfer of ovine embryos. Theriogenology 33: 330 (abstr.).CrossRefGoogle Scholar
Thomson, J.G., Bell, A.C.S., McMillan, W.H., Peterson, A.J. and Tervit, H.R. 1995. Donor and recipient ewe factors affecting in vitro development and post-transfer survival of cultured sheep embryos. Animal Reproduction Science 40: 269–279.CrossRefGoogle Scholar
Thompson, J.G., Simpson, A.C., Jammes, R.W., Tervit, H.R., Asher, G.W. and Peterson, A.J. 1992. Timing of the LH peak and ovulations in superovulated Coopworth ewes synchronised with progesterone-containing CIDR devices. Proceedings of the New Zealand Society of Animal Production 52: 171–174.Google Scholar
Thompson, J.G.E., Simpson, A.C. and Tervit, H.R. 1990. The application of progesterone-containing CIDR™ devices to superovulated ewes. Theriogenology 33: 1297–1304.CrossRefGoogle Scholar
Torres, S., Cognie, Y. and Colas, G. 1987. Transfer of superovulated sheep embryos obtained with different FSH-P. Theriogenology 27: 407–419.CrossRefGoogle ScholarPubMed
Walker, S.K., Warnes, G.M., Quinn, P., Seamark, R.F. and Smith, D.H. 1985. Laparoscopic technique for the transfer of embryos in sheep. Australian Veterinary Journal 62: 105–106.CrossRefGoogle ScholarPubMed
Wierzchos, E., Tischner, M. and Muffii, M. 1992. Superovulation of a low fecundity sheep breed using a porcine gonadotrophin extract with a defined LH content. Theriogenology 38: 147–152.CrossRefGoogle ScholarPubMed
Woolliams, J.A., Luo, Z.W., Villanueva, B., Waddington, D., Broadbent, P.J., McKelvey, W.A.C. and Robinson, J.J. 1995. Analysis of factors affecting superovulat responses in ruminants. Theriogenology 124: 61–70.Google Scholar