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Amino acid requirements of growing pigs 2. Identification of the limiting amino acid(s) in a low-protein diet supplemented with lysine

Published online by Cambridge University Press:  02 September 2010

A. J. Taylor
Affiliation:
University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leicestershire LE12 5RD
D. J. A. Cole
Affiliation:
University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leicestershire LE12 5RD
D. Lewis
Affiliation:
University of Nottingham School of Agriculture, Sutton Bonington, Loughborough, Leicestershire LE12 5RD
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Abstract

A basal diet containing 120 g crude protein per kg and 9g lysine per kg, and previously shown to be limiting in one or more essential amino acids and/or non-essential nitrogen, was examined. It was fed either alone to growing female pigs from 25 kg to 55 kg live weight or in combination with four supplements of synthetic amino acids each containing three out of isoleucine, methionine, threonine and tryptophan. A control diet containing 140 g crude protein per kg and 9g lysine per kg was also included. Blood samples were collected at 40 kg live weight in order to examine the influence of dietary treatments on blood metabolites. Results for growth performance, carcass composition and blood urea indicated that threonine was the first limiting amino acid in the basal diet. Plasma free amino acids gave no clear trend. Growth performance and carcass composition were unaffected by supplementation of the diet with glycine indicating that the dietary supply of non-essential nitrogen was adequate.

Type
Research Article
Copyright
Copyright © British Society of Animal Science 1981

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References

REFERENCES

Berry, T. H., Becker, D. E., Rasmussen, O. G., Jensen, A. H. and Norton, H. W. 1962. The limiting amino acids in soybean protein. J. Anim. Sci. 21: 558561.CrossRefGoogle Scholar
Berry, T. H., Combs, G. E., Wallace, H. D. and Robbins, R. C. 1966. Responses of the growing pig to alterations in the amino acid pattern of isolated soybean protein. J. Anim. Sci. 25: 722728.CrossRefGoogle ScholarPubMed
Conway, E. J. 1962. Microdiffusion Analysis and Volumetric Error. 3rd ed. Crosby Lockwood, London.Google Scholar
Denckla, W. D. and Dewey, H. K. 1967. The determination of tryptophan in plasma, liver and urine. J. Lab. din. Med. 69: 160169.Google ScholarPubMed
Eggum, B. O. 1970. Blood urea measurement as a technique for assessing protein quality. Br. J. Nutr. 24: 983988.CrossRefGoogle ScholarPubMed
Fuller, M. F., Livingstone, R. M. and Mennie, I. 1974. Progessive supplementation of barley with amino acids in a pig diet. Proc. Nutr. Soc. 33: 91A93A (Abstr.).Google Scholar
Fuller, M. F., Livingstone, R. M. and Mennie, I. 1975. Responses of growing pigs to combinations of essential amino acids. Proc. Nutr. Soc. 34: 99A100A (Abstr.).Google ScholarPubMed
Hamilton, P. B. 1962. Ion-exchange chromatography of amino acids—microdetermination of free amino acids in serum. Ann. N.Y. Acad. Sci. 102: 5575.CrossRefGoogle ScholarPubMed
Harter, H. L. 1960. Critical values for Duncan's new multiple range test. Biometrics 16: 671685.CrossRefGoogle Scholar
Henry, Y. and Rerat, A. 1970. Estimation du besoin en threonine diez le pore femelle entre 20 et 50 kg de poids vif. Journees de la Recherche Porcine en France, pp. 7378.Google Scholar
Maner, J. H., Pond, W. G. and Loosli, J. K. 1961. Utilization of soybean protein by baby pigs and by rats. J. Anim. Sci. 20: 614620.CrossRefGoogle Scholar
Muller, Z., Kozel, V., Bauer, B., Strunc, M. and Moravec, J. 1967. Enrichment of cereal diets for fattening pigs from 35 kg live weight with lysine, threonine, tryptophan and methionine. Biol. Chem. vyž. Zvířat 5: 399410.Google Scholar
Rolls, B. A., Porter, J. W. G. and Westgarth, D. R. 1972. The course of digestion of different food proteins in the rat. 3. The absorption of proteins given alone and with supplements of their limiting amino acids. Br. J. Nutr. 28: 283293.CrossRefGoogle ScholarPubMed
Taylor, A. J., Cole, D. J. A. and Lewis, D. 1979. Amino acid requirements of growing pigs. 1. Effects of reducing protein level in diets containing high levels of lysine. Anim. Prod. 29: 327338.Google Scholar