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Adrenal responsiveness of reindeer (Rangifer tarandus tarandus) to intravenously administered ACTH

Published online by Cambridge University Press:  09 March 2007

H. Säkkinen ,
J. Tornberg ,
P. J. Goddard ,
E. Eloranta ,
E. Dahl ,
E. Ropstad  and
S. Saarela
H. Säkkinen*
Affiliation:
Department of Biology, University of Oulu, PO Box 3000, FIN-90014 University of Oulu, Finland
J. Tornberg
Affiliation:
Department of Biology, University of Oulu, PO Box 3000, FIN-90014 University of Oulu, Finland
P. J. Goddard
Affiliation:
Macaulay Institute, Craigiebuckler, Aberdeen AB15 8QH, UK
E. Eloranta
Affiliation:
Department of Physiology, University of Oulu, PO Box 5000, FIN-90014 University of Oulu, Finland
E. Dahl
Affiliation:
Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, PO Box 8146 Dep, NO-0033, Oslo, Norway
E. Ropstad
Affiliation:
Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, PO Box 8146 Dep, NO-0033, Oslo, Norway
S. Saarela
Affiliation:
Department of Biology, University of Oulu, PO Box 3000, FIN-90014 University of Oulu, Finland
*
†E-mail: [email protected]
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Abstract

Plasma cortisol concentrations were determined from the blood of eight mature female reindeer (Rangifer tarandus tarandus) after an intravenous injection of either saline (control) or 100, 250 or 500 μg of synthetic ACTH. Blood samples were collected at 0, 2, 4, 6, 8, 10, 15, 20, 25, 30, 45, 60, 75, 90, 120, 150, and 180 min after the injections. The aims were to determine the appropriate dose of ACTH for adrenal stimulation tests, to define the dose level of ACTH which elicited a maximal cortisol response and to describe the range of blood cortisol concentrations for reference when evaluating the stress responses of reindeer.

The mean plasma cortisol concentrations (s.e.) at the zero sample times (t0) of the control and the ACTH treatments varied between 93·4 (11·8) and 132·5 (18·1) nmol/l. The total plasma cortisol response (area under curve, AUC, nmol/l × min) increased with increasing dose of ACTH (P < 0·001). The AUC of the control treatment was significantly smaller than of the ACTH treatments (P < 0·001). The highest dose of ACTH (500 μg) gave a significantly bigger AUC than the lowest dose (100 μg) (P = 0·008). The maximal plasma cortisol concentrations (CMAX) were achieved within 60 min of the ACTH injections. The ranges of individual CMAX were 59·0 to 136·8 nmol/l for the control treatment, and 110·0 to 252·0, 152·0 to 247·5 and 135·1 to 257·1 nmol/l for 100, 250 and 500 μg ACTH, respectively. The difference in CMAX was significant between the control treatment and the ACTH treatments (P < 0·001) but not between the different doses of ACTH. The plasma cortisol concentrations at the end of the observation period at t180 were not significantly affected by the ACTH treatment (P > 0·05).

In conclusion, the 100-μg dose of ACTH was sufficient to produce a significant cortisol response compared with the control treatment. Increasing the dose did not increase the maximal response, but tended to elongate the response profile. The blood sampling frequency used in the study was found suitable for detection of the cortisol response in reindeer.

Keywords

ACTHadrenal glandsdeerhydrocortisonereindeerstress
Type
Research Article
Information
Animal Science , Volume 81 , Issue 3 , December 2005 , pp. 399 - 402
Copyright
Copyright © British Society of Animal Science 2005

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References

Broom, D. M. and Johnson, K. E. 1993. Stress and animal welfare. Chapman and Hall, London.CrossRefGoogle Scholar
Bubenik, G. A. and Bartos, L. 1993. Cortisol levels in red deer (Cervus elaphus) and fallow deer (Dama dama) after an acute ACTH administration. Canadian Journal of Zoology 71: 2258–2261.CrossRefGoogle Scholar
Bubenik, G. A., Brown, R. D. and Schams, D. 1991. Antler cycle and endocrine parameters in male axis deer (Axis axis): seasonal levels of LH, FSH, testosterone, and prolactin and results of GnRH and ACTH challenge tests. Comparative Biochemistry and Physiology 99A: 645–650.CrossRefGoogle ScholarPubMed
Bubenik, G. A., Reyes, E. and Schams, D. 2000. Pudu, the smallest deer of the world: 10 years of endocrine studies of Southern pudu (Pudu puda) in Chile. Zeitschrift für Jagdwissenschaft 46: 129–138.Google Scholar
Bubenik, G. A. and Reyes-Toledo, E. 1994. Plasma levels of cortisol, testosterone and growth hormone in pudu (Pudu puda Molina) after ACTH administration. Comparative Biochemistry and Physiology 107A: 523–527.CrossRefGoogle Scholar
Bubenik, G. A., Schwartz, C. C. and Carnes, J. 1994. Cortisol concentrations in male Alaskan moose (Alces a. gigas) after exogenous ACTH administration. Alces 30: 65–69.Google Scholar
Ferre, I., Goddard, P. J., Macdonald, A. J., Littlewood, C. A. and Duff, E. I. 1998. Effect of method of blood sample collection on adrenal activity in farmed red deer and sheep following administration of ACTH. Animal Science 67: 157–164.CrossRefGoogle Scholar
Goddard, P. J., Gaskin, G. J. and Macdonald, A. J. 1998. Automatic blood sampling equipment for use in studies of animal physiology. Animal Science 66: 769–775.CrossRefGoogle Scholar
Goddard, P. J., Rhind, S. M., Hamilton, W. J., Macdonald, A. J., Fawcett, A. R., Soanes, C. and McMillen, S. R. 1994. The adrenocorticotrophic hormone stimulation test: its potential use and limitations in red deer (Cervus elaphus). Canadian Journal of Zoology 72: 1826–1830.CrossRefGoogle Scholar
Hyvärinen, H., Helle, T., Nieminen, M., Väyrynen, P. and Väyrynen, R. 1976. Some effects of handling reindeer during gatherings on the composition of their blood. Animal Production 22: 105–114.Google Scholar
Ingram, J. R., Crockford, J. N. and Matthews, L. R. 1999. Ultradian, circadian and seasonal rhythms in cortisol secretion and adrenal responsiveness to ACTH and yarding in unrestrained red deer (Cervus elaphus) stags. Journal of Endocrinology 162: 289–300.CrossRefGoogle Scholar
Ingram, J. R., Matthews, L. R., Carragher, J. F. and Schaare, P. R. 1997. Plasma cortisol responses to remote adrenocorticotropic hormone (ACTH) infusion in free-ranging red deer (Cervus elaphus). Domestic Animal Endocrinology 14: 63–71.CrossRefGoogle Scholar
Levine, S. 1985. A definition of stress? In Animal stress (ed. Moberg, G. P.), pp. 51–69. The Williams and Wilkins Company, Baltimore.CrossRefGoogle Scholar
Littell, R. C., Milliken, G. A., Stroup, W. W. and Wolfinger, R. D. 1996. SAS system for mixed models. Statistical Analysis Systems Institute Inc., Cary, NC.Google Scholar
Malmfors, G. and Wiklund, E. 1996. Pre-slaughter handling of reindeer–effects on meat quality. Meat Science 43: S257–S264.CrossRefGoogle Scholar
Matteri, R. L. and Moberg, G. P. 1982. Effect of cortisol or adrenocorticotrophin on release of luteinizing hormone induced by luteinizing hormone releasing hormone in the dairy heifer. Journal of Endocrinology 92: 141–146.CrossRefGoogle ScholarPubMed
Moberg, G. P. 1985. Biological response to stress: key to assessment of animal well-being? In Animal stress (ed. Moberg, G. P.), pp. 27–49. The Williams and Wilkins Company, Baltimore.CrossRefGoogle Scholar
Moberg, G. P. 1991. How behavioural stress disrupts the endocrine control of reproduction in domestic animals. Journal of Dairy Science 74: 304–311.CrossRefGoogle ScholarPubMed
Rehbinder, C., Edqvist, L.-E., Lundström, K. and Villafañe, F. 1982. A field study of management stress in reindeer (Rangifer tarandus L.). Rangifer 2: 2–21.CrossRefGoogle Scholar
Roth, J. A. 1985. Cortisol as a mediator of stress-associated immunosuppression in cattle. In Animal stress (ed. Moberg, G. P.), pp. 225–241. The Williams and Wilkins Company, Baltimore.CrossRefGoogle Scholar
Säkkinen, H., Tornberg, J., Goddard, P. J., Eloranta, E., Ropstad, E. and Saarela, S. 2004. The effect of blood sampling method on indicators of physiological stress in reindeer (Rangifer tarandus tarandus). Domestic Animal Endocrinology 26: 87–98.CrossRefGoogle ScholarPubMed
Simensen, E., Olson, L. D., Vanjonack, W. J., Johnson, H. D. and Ryan, M. P. 1978. Determination of corticosterone concentration in plasma of turkeys using radioimmunoassay. Poultry Science 57: 1701–1704.CrossRefGoogle Scholar
Smith, J. H. and Bubenik, G. A. 1990. Plasma concentrations of glucocorticoids in white-tailed deer: the effect of acute ACTH and dexamethasone administration. Canadian Journal of Zoology 68: 2123–2129.CrossRefGoogle Scholar
Tillbrook, A. J., Canny, B. J., Serapiglia, M. D., Ambrose, T. J. and Clarke, I. J. 1999. Suppression of the secretion of luteinizing hormone due to isolation/restraint stress in gonadectomized rams and ewes is influenced by sex steroids. Journal of Endocrinology 160: 469–481.CrossRefGoogle Scholar
Turner, A. I., Canny, B. J., Hobbs, R. J., Bond, J. D., Clarke, I. J. and Tillbrook, A. J. 2002. Influence of sex and gonadal status of sheep on cortisol secretion in response to ACTH and on cortisol and LH secretion in response to stress: importance of different stressors. Journal of Endocrinology 173: 113–122.CrossRefGoogle ScholarPubMed
Van Mourik, S. and Stelmasiak, T. 1984. Adrenal response to ACTH stimulation in rusa deer (Cervus rusa timorensis). Comparative Biochemistry and Physiology 79A: 581–584.CrossRefGoogle ScholarPubMed
Wiklund, E., Andersson, A., Malmfors, G. and Lundström, K. 1996. Muscle glycogen levels and blood metabolites in reindeer (Rangifer tarandus tarandus L.) after transport and lairage. Meat Science 42: 133–144.CrossRefGoogle ScholarPubMed