Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-23T12:29:02.405Z Has data issue: false hasContentIssue false

Survival in Vitro of sheep eggs

Published online by Cambridge University Press:  02 September 2010

J. L. Hancock
Affiliation:
A.R.C. Animal Breeding Research Organisation, Edinburgh 9
Get access

Summary

Transfers were made to recipient ewes of sheep eggs stored in serum at 37° C. for up to 5 hr. (fresh eggs), in serum at room temperature for 24 to 48 hr. (stored eggs), or in Whitten's modified Krebs-Ringer-bicarbonate medium for 24 to 48 hr. (cultured eggs). The success of transfer was judged from the presence of embryos at autopsy 25–30 days after transfer.

Fifteen of 42 fresh eggs, 11 of 23 stored eggs and 3 of 15 cultured eggs were found to have developed after transfer.

The findings are discussed.

Type
Research Article
Copyright
Copyright © British Society of Animal Science 1963

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Averell, R. L. W., 1956. The transfer and storage of sheep ova. Proc. IIIrd. int. Congr. Anim. Reprod., Cambridge, 3: 7.Google Scholar
Biggers, J. D., Finn, C. A., McLaren, A., & Woolf, B., 1962. Long term reproductive performance of female mice. II. Variation of litter size with parity. J. Reprod. Fert., 3: 313.CrossRefGoogle ScholarPubMed
Boyd, J. D., Hamilton, W. J., & Hammond, J. Jr., 1944. Transuterine (internal) migration of ovum in sheep and other mammals. J. Anat. Lond., 78: 5.Google ScholarPubMed
Hancock, J. L., & Hoveix, G. J. R., 1961a. Transfer of sheep ova. J. Reprod. Fert., 2: 295.CrossRefGoogle ScholarPubMed
Hancock, J. L., & Hoveix, G. J. R., 1961b. Transfer of sheep ova. Abstract. J. Reprod. Fert., 2: 520.CrossRefGoogle Scholar
McLaren, A., & Biggers, J. D., 1958. Successful development and birth of mice cultivated in vitro as early embryos. Nature, Lond., 182: 877.CrossRefGoogle ScholarPubMed
Quinlan, J., Mare, G. S., & Roux, L. L., 1932. The vitality of the spermatozoa in the genital tract of the Merino ewe with special reference to its application in breeding. 18th Rep. Div. Vet. Sen. Onderst. Pt. 2, 831.Google Scholar
Robinson, T. J., 1950. The control of fertility in sheep. II. The augmentation of fertility by gonadotrophin treatment of the ewe in the normal breeding season. J. agric. Sci., 41: 6.CrossRefGoogle Scholar
Tarkowski, A. K., 1961. Mouse chimaeras developed from fused eggs. Nature, Lond., 190: 857.CrossRefGoogle ScholarPubMed