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Sampling of digestive contents by ileal cannulation in the rabbit

Published online by Cambridge University Press:  02 September 2010

T. Gidenne ,
T. Bouyssou  and
Y. Ruckebusch
T. Gidenne
Affiliation:
Institut National de la Recherche Agronomique, Laboratoire de Recherches sur l'Elevage du Lapin, BP 27-31326 Castanet-Tolosan Cedex, France
T. Bouyssou
Affiliation:
Ecole Nationale Vétérinaire, Laboratoire de Physiologie, 23 Chemin des Capelles — 31076 Toulouse Cedex, France
Y. Ruckebusch
Affiliation:
Ecole Nationale Vétérinaire, Laboratoire de Physiologie, 23 Chemin des Capelles — 31076 Toulouse Cedex, France
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Abstract

A technique is described for the collection of digestive contents with a glass T-shaped ileal cannula, in rabbits fed ad libitum. Repeated samples of ileal contents were easily obtained by gravity from conscious animals lying in a hammock for 1 h, twice a week, over a period of 2 months. Analysis of the ileal contents showed no diurnal variations in concentrations of crude protein, neutral-detergent fibre, ammonia, and total short-chain volatile fatty acids. The fresh weight output was maximal at 09.00 h and minimal at 03.00 h. The transit time of phenol sulphone phthalein between mouth and ileum was 75 (s.d. 14) min and did not vary according to the time of day when measured (08.00 to 12.00 h, 14.00 to 18.00 h, 20.00 to 24.00 h).

Type
Research Article
Information
Animal Production , Volume 46 , Issue 1 , February 1988 , pp. 147 - 151
Copyright
Copyright © British Society of Animal Science 1988

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References

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