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Influence of anabolic treatments on luteinizing hormone and testosterone secretion in bulls

Published online by Cambridge University Press:  02 September 2010

J. Fabry ,
R. Renaville  and
A. Burny
J. Fabry
Affiliation:
Station de Zootechnie, Centre de Recherches Agronomiques Chemin de Liroux, 11, B-5800 Gembloux, Belgium
R. Renaville
Affiliation:
Chaire de Physiologie Animale et de Zootechnie, Faculté des Sciences Agronomiques, Passage des Déportes, 2, B-5800 Gembloux, Belgium
A. Burny
Affiliation:
Chaire de Physiologie Animale et de Zootechnie, Faculté des Sciences Agronomiques, Passage des Déportes, 2, B-5800 Gembloux, Belgium
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Abstract

Fourteen growing Belgian White Blue bulls were assigned to three groups: five controls (C); five bulls implanted with 140 mg trenbolone acetate and 36 mg Zeranol (TBA-Z); and four bulls implanted with 140 mg trenbolone acetate and 20 mg oestradiol-17β (TBA-E2). Testosterone (T) and luteinizing hormone (LH) levels in blood plasma were determined at day 0, 30 and 60. Mean plasma LH concentrations measured by repeated sampling over a 10-h period were similar at day 0, 30 and 60 in the three groups. At days 30 and 60, T levels were significantly lowered in treated bulls: namely 2·0 (s.d. 0·7) μg/1 v. 2·7 (s.d. 0·5) μg/1; 0·7 (s.d. 0·1) μg/1 v. 1·0 (s.d. 0·3) μg/1; 0·5 (s.d. 0·1) μg/l v. 0·4 (s.d. 0·2) μg/1 in the C, TBA-Z and TBA-E2 groups respectively. Moreover, anabolic compounds completely abolished pulsatile liberation of LH and T.

In control bulls intravenous injection of 0·5 ng/kg body weight of LH releasing hormone (LHRH) caused the release of a large quantity of LH (10 to 13 times the basal level after 10 min) followed by an increase in plasma T levels. On the other hand, LH and T responses to LHRH were reduced by TBA-E2 and TBA-Z treatments. A delay in LH response to LHRH was apparent 6 days after anabolic treatment. However, the total amount of LH liberated during the test was increased. The second effect was a quantitative reduction at days 20, 30 and 60 in LH and T secretion after LHRH. The modification of the hormonal pattern was quicker and more pronounced in TBA-E2 than in TBA-Z animals. Correlation was negative (r = −0·68; P < 0·05) between the mean daily gain during the experimental period and the total quantity of T liberated by the testis after LHRH stimulation at day 60. It was concluded that administration of trenbolone acetate plus Zeranol or trenbolone acetate plus oestradiol-17β in pubertal bulls reduced the LH and T responses to LHRH and abolished the pulsatile LH and T secretions.

Type
Research Article
Information
Animal Production , Volume 39 , Issue 3 , December 1984 , pp. 345 - 354
Copyright
Copyright © British Society of Animal Science 1984

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References

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