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Evaluation of the spot urine sampling technique to assess urinary purine derivative excretion in lactating dairy cows

Published online by Cambridge University Press:  02 September 2010

K. J. Shingfield  and
N. W. Offer
K. J. Shingfield
Affiliation:
Grassland and Ruminant Science Department, Scottish Agricultural College, Auchincruive, Ayr KA6 5HW
N. W. Offer
Affiliation:
Grassland and Ruminant Science Department, Scottish Agricultural College, Auchincruive, Ayr KA6 5HW
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Abstract

The potential of the spot urine sampling technique as an alternative to performing a total urine collection was evaluated. Twelve multiparous Holstein-Friesian cows were given two experimental diets in a complete change-over design using two 14-day experimental periods. Experimental diets were either silage offered ad libitum with 7 kg fresh weight concentrate supplement as a single meal (SF), or a complete diet formulated from the same ingredients with a similar foragexoncentrate ratio (CD). Total urine collections were performed every 2 h on days 11 and 14 of each experimental period. Subsamples of urine were stored at 20°C and subsequently analysed by high-performace liquid chromatography. Daily allantoin and purine derivative (PD) excretion were highly correlated (r = 0·995, no. = 48, P < 0·001). PD and creatinine excretion during each 2-h interval depended on time of collection (PD, P < 0·001 and creatinine, P < 0·05) and on cow (P < 0·01) but were unaffected by sampling day or treatment. Diurnal variations in the molar ratio ofPD or allantoin to creatinine (PD/c and Ale, respectively) followed similar diurnal patterns as observed for PD and allantoin excretion. The data were used to assess the error of prediction of daily mean PD/c or Ale ratios. Three spot sampling regimens (based on the collection of four 4-h samples, three 8-h samples or two 12-h samples) and also on either single or 2-day urine collections were evaluated. Collection of multiple samples within a day was more reliable than collecting fewer samples over several days. Prediction errors were greater for SF compared with CD. Even the most intensive sampling regimen did not allow an acceptable prediction of daily mean PDIc or Ale ratio, minimum r values for PDIc and Ale ratios were 0·098, 0·136 and 0·547, 0·579 for SF and CD, respectively. Furthermore, daily mean PDIc and Ale ratios proved poor predictors of daily PD and allantoin excretion (r values of 0·69 and 0·72, respectively). Total urine collection appears necessary to assess accurately daily PD excretion in dairy cows.

Keywords

creatininedairy cowsfeeding frequencypurines
Type
Research Article
Information
Animal Science , Volume 66 , Issue 3 , June 1998 , pp. 557 - 568
Copyright
Copyright © British Society of Animal Science 1998

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References

Agnew, K. W., Mayne, C. S. and Doherty, J. G. 1996. An examination of the effect of method and level of concentrate feeding on milk production in dairy cows offered a grass silage-based diet. Animal Science 63: 21–31.CrossRefGoogle Scholar
Agricultural and Food Research Council. 1992. Technical Committee on Responses to Nutrients, report number 9. Nutritive requirements of ruminant animals: protein. Nutrition Abstracts and Reviews (Series B) 62: 788–835.Google Scholar
Agricultural Research Council. 1984. The nutrient requirements of ruminant livestock. Supplement 1. Commonwealth Agricultural Bureaux, Farningham Royal, Slough.Google Scholar
Alexander, R. H. 1969. The establishment of a laboratory procedure for the ‘in-vitro’ determination of digestibility. Research bulletin no. 42.Google Scholar
Antoniewicz, A., Heinemann, W. W. and Hanks, E. M. 1981. Effect of level of feed intake and body mass on allantoin excretion and the allantoin to creatinine ratio in the urine of sheep. Roczniki Naukowe Zootechniki 8: 49–65.Google Scholar
Balcells, J., Parker, D. S., Guada, J. A. and Peiro, J. M. 1992. Simultaneous analysis of allantoin and oxypurines in biological fluids by high performance liquid chromatography. Journal of Chromatography — Biomedical Applications 575: 153–157.CrossRefGoogle ScholarPubMed
Bates, D. B. and Bergen, W. G. 1984. Effect of nutrient limitation on the RNA/protein ratio of several rumen bacteria. Canadian Journal of Animal Science 64: (supplement 45–46.CrossRefGoogle Scholar
Cecava, M. J., Merchen, N. R., Gay, L. C. and Berger, L. L. 1990. Composition of ruminal bacteria harvested from steers as influenced by dietary energy level, feeding frequency, and isolation techniques. Journal of Dairy Science 73: 2480–2488.CrossRefGoogle ScholarPubMed
Chen, X. B., Grubic, G., Orskov, E. R. and Osuji, P. 1992. Effect of feeding frequency on diurnal variation in plasma and urinary purine derivatives in steers. Animal Production 55: 185–191.Google Scholar
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. and Brown, D. S. 1990a. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63: 131–142.CrossRefGoogle ScholarPubMed
Chen, X. B., Mejia, A. T., Kyle, D. J. and Orskov, E. R. 1995. Evaluation of the use of the purine derivative: creatinine ratio in spot urine and plasma samples as an index of microbial protein supply in ruminants: studies in sheep. Journal of Agricultural Science, Cambridge 125: 137–142CrossRefGoogle Scholar
Chen, X. B., Ørskov, E. R. and Hovell, F. D. DeB. 1990b. Excretion of purine derivatives by ruminants: endogenous excretion, differences between cattle and sheep. British Journal of Nutrition 63: 121–129.CrossRefGoogle ScholarPubMed
Chetal, U., Mehra, U. R., Nath, K. and Ranjhan, S. K. 1975. On the variation of urinary creatinine in buffalo calves and the effect of dietary protein intake on urinary creatinine, creatinine-nitrogen ratio and creatinine coefficient. Journal of Agricultural Science, Cambridge 84: 1–5.CrossRefGoogle Scholar
Craig, W. M., Brown, D. R., Broderick, G. A. and Ricker, D. B. 1987. Post-prandial compositional changes of fluid and particle associated ruminal micro-organisms. Journal of Animal Science 65: 1042–1048.CrossRefGoogle Scholar
Daniels, Z. M., Chen, X. B., Kyle, D. J., Sinclair, K. and Ørskov, E. R. 1994. Purine derivatives in urine and plasma of lactating cows given different levels of food intake. Animal Production 58: 453 (abstr.).Google Scholar
De Groot, T. H. and Aafjes, J. H. 1960. On the constancy of creatinine excretion in the urine of the dairy cow. British Veterinary Journal 116: 409–418.CrossRefGoogle Scholar
Dewhurst, R. J. 1989. Studies on energy and nitrogen metabolism in the rumen — investigation of less invasive techniques for these studies. Ph.D. thesis, University of Bristol.Google Scholar
Dewhurst, R. J., Mitton, A. M., Offer, N. W. and Thomas, C. 1996. Effects of the composition of grass silages on milk production and nitrogen utilization by dairy cows. Animal Science 62: 25–34.CrossRefGoogle Scholar
Dewhurst, R. J. and Webster, A. J. F. 1992. Effects of diet, level of intake, sodium bicarbonate and monensin on urinary allantoin excretion in sheep. British Journal of Nutrition 67: 345–353.CrossRefGoogle ScholarPubMed
Dolan, J. W. and Snyder, L. R. 1989. Troubleshooting LC systems. A comprehensive approach to troubleshooting LC equipment and separations. Humana Press, Clifton, New Jersey.CrossRefGoogle Scholar
Erb, R. E., Surve, A. H., Randal, R. D. and Carverick, H. A. 1977. Urinary creatinine as an index of urinary excretion of estrogen in cows prepartum and postpartum. Journal of Dairy Science 60: 825–828.CrossRefGoogle ScholarPubMed
Gonda, H. L. and Lindberg, J. E. 1994. Evaluation of dietary nitrogen utilisation in dairy cows based on urea concentrations in blood, urine and milk, and on urinary concentrations of purine derivatives. Ada Agricultae Scandinavica, Section A, Animal Science 44: 236–245.Google Scholar
Greger, R., Lang, F. and Deetjen, P. 1976. Renal excretion of purine metabolites, urate and allantoin by the mammalian kidney. In International review of physiology kidney and urinary tract physiology II, volume II (ed. Thurau, K.), pp. 257–281.Google Scholar
Lawes Agricultural Trust. 1993. GENSTAT 5 release 3, reference manual. Clarendon Press, Oxford.Google Scholar
Lindberg, J. E. and Jacobsson, K.-G. 1990. Nitrogen and purine metabolism at varying energy and protein supplies in sheep sustained on intragastric infusion. British Journal of Nutrition 64: 359–370.CrossRefGoogle ScholarPubMed
Lux, O., Naidoo, D. and Salonikas, C. 1992. Improved HPLC method for the simultaneous measurement of allantoin and uric acid in plasma. Annals of Clinical Biochemistry 29: 674–675.CrossRefGoogle ScholarPubMed
McAllan, A. B. 1982. The fate of nucleic acids in ruminants. Proceedings of the Nutrition Society 41: 309–317.CrossRefGoogle ScholarPubMed
McSweeney, C. S. 1986. An omaso-abomasal cannula used to assess efflux from the omasum of sheep. Australian Veterinary Journal 63: 332–334.CrossRefGoogle ScholarPubMed
Malestein, A., van't, Klooster A. T., Counotte, G. H. M. and Prins, R. A. 1981. Concentrate feeding and ruminal fermentation. 1. Influence of the frequency of feeding concentrates on rumen acid composition, feed intake and milk production. Netherlands Journal of Agricultural Science 29: 239–248.CrossRefGoogle Scholar
Ministry of Agriculture, Fisheries and Food. 1975. Energy allowances and feeding systems for ruminants. Technical bulletin 33. Ministry of Agriculture, Fisheries and Food, London.Google Scholar
Minitab Inc. 1980. Minitab data analysis software. Pennsylvania State University, Pennsylvania.Google Scholar
Moorby, J. M. and Dewhurst, R. J. 1993. Diurnal variation in urinary purine derivative excretion of dairy cows on different feeding patterns. Animal Production 56: 464 (abstr.).Google Scholar
Moseley, G. and Jones, J. R. 1984. The physical digestion of perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) in the foregut of sheep. British Journal of Nutrition 52: 381–390.CrossRefGoogle ScholarPubMed
Mould, F. L. and Orskov, E. R. 1983. Manipulation of rumen fluid pH and its influence on cellulolysis in-sacco, dry matter degradation and the microflora of sheep offered either hay or concentrate. Animal Feed Science and Technology 10: 1–14.CrossRefGoogle Scholar
Mould, F. L., Orskov, E. R. and Mann, S. O. 1983. Associative effects of mixed feeds. 1. Effects of type and level of supplement and the influence of the minimal pH on cellulolysis in-vivo and dry matter digestion of various roughages. Animal Feed Science and Technology 10: 15–30.CrossRefGoogle Scholar
Narayanan, S. and Appleton, H. D. 1980. Creatinine: a review. Journal of Clinical Chemistry 26: 1119–1126.CrossRefGoogle ScholarPubMed
Nikolic, J. A. and Jovanovic, M. 1973. Preliminary study on the use of different methods for determining the proportion of bacterial nitrogen in the total nitrogen of rumen contents. Journal of Agricultural Science, Cambridge 81: 1–7.CrossRefGoogle Scholar
Offer, N. W. 1973. Utilisation of dietary nitrogen by the sheep. Ph.D. thesis, University of Wales.Google Scholar
Phipps, R. H., Bines, J. A., Fulford, R. J. and Weller, R. F. 1984. Complete diets for dairy cows: a comparison between complete diets and separate ingredients. Journal of Agricultural Science, Cambridge 103: 171–180.CrossRefGoogle Scholar
Puchala, R. and Kulasek, G. W. 1992. Estimation of microbial protein from the rumen of sheep using microbial nucleic acid and urinary excretion of purine derivatives. Canadian Journal of Animal Science 72: 821–830.CrossRefGoogle Scholar
Puchala, R., Shelford, J. A., Barej, W., Kulasek, G. W., Pior, H., Keyserlingk, M. V. and Makoni, N. 1993. Urinary excretion of pseudouridine and purine metabolites in ruminants. Journal of Animal Physiology and Animal Nutrition 69: 186–193.CrossRefGoogle Scholar
Shingfield, K. J. 1996. Renal and mammary purine derivative excretion in Holstein/Friesian dairy cows:N—its potential as a non-invasive index of protein metabolism. Ph.D. thesis, University of Glasgow.CrossRefGoogle Scholar
Stanley, R. W. and Morita, K. 1967. Effect of frequency and method of feeding on performance of lactating dairy cattle. Journal of Dairy Science 50: 585–586.CrossRefGoogle Scholar
Susmel, P., Spanghero, M., Stefanon, B., Mills, C. R. and Plazzotta, E. 1994a. Digestibility and allantoin excretion in cows fed diets differing in nitrogen content. Livestock Production Science 39: 97–99.CrossRefGoogle Scholar
Susmel, P., Stefanon, B., Plazzotta, C. R., Spanghero, M. and Mills, C. R. 1994b. The effect of energy and protein intake on the excretion of purine derivatives. Journal of Agricultural Science, Cambridge 123: 257–265.CrossRefGoogle Scholar
Thomas, P. C., Robertson, S., Chamberlain, D. G., Livingstone, R. M., Garthwaite, P. H., Dewey, P. J. S., Smart, R. and White, C. 1988. Predicting the metabolisable energy (ME) content of compound feeds for ruminants. In Recent advances in animal nutrition (ed. Haresign, W. and Cole, D. J. A.), pp. 127–146. Butterworths, London.Google Scholar
Tiermeyer, W. and Giesecke, D. 1982. Quantitative determination of allantoin in biological fluids by reversedphase-high-pressure liquid chromatography. Analytical Biochemistry 123: 11–13.CrossRefGoogle Scholar
Tilley, J. M. A. and Terry, R. A. 1963. A two-stage technique for the in-vitro digestion of forage crops. Journal of the British Grassland Society 18: 104.CrossRefGoogle Scholar
Topps, R. H. and Elliot, R. C. 1965. Relationship between concentrations of ruminal nucleic acid and excretion of purine derivatives by sheep. Nature 205: 498–499.CrossRefGoogle Scholar
Verbic, J., Chen, X. B., Macleod, N. A. and Orskov, E. R. 1990. Excretion of purine derivatives by ruminants. Effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science, Cambridge 114: 243–248.CrossRefGoogle Scholar