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Effects of open field testing and associated handling v. handling alone on the adrenocortical reactivity of piglets around weaning

Published online by Cambridge University Press:  18 August 2016

M. S. Herskin  and
K. H. Jensen
M. S. Herskin
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Health and Welfare, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
K. H. Jensen
Affiliation:
Danish Institute of Agricultural Sciences, Department of Animal Health and Welfare, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
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Abstract

Effects of open field testing and associated handling (including blood sampling) v. handling (with blood sampling) alone on adrenocortical reactivity were investigated in piglets around weaning. After weaning at day 28, piglets were mixed to form eight replicates with three litter each and kept on slatted floors. Treatments were open field/novel object testing and associated handling (OFT + H) v. handling alone (H) (no. = 40). Testing took place on the following days (weaning day 0): -4, 0, 1, 4 and 8 (no. = 16). The open field/novel object test lasted 10 min and blood was sampled by venipuncture before and after the test and/or handling procedures. In the first blood sample, plasma concentration of cortisol did not differ between OFT + H and H piglets. In the second blood sample, however, OFT + H piglets had a higher concentration of cortisol and a larger increase in cortisol than H piglets (P < 0·01). In the first blood sample the concentration of cortisol was affected by day (P < 0·05), with concentration on day -4 being lower than those on day 0, 1, and 4 and the concentration on day 8 was lower than the concentration on day 0 as well. However, no differences were found between days for the second blood sample nor the adrenocortical reactivity. In conclusion, the increase in pre-treatment cortisol in the first 4 days after weaning as well as the lack of changes in the adrenocortical reactivity after weaning suggest that the temporal development of HPA activity and reactivity in piglets after weaning are comparable with other, more standardized long-term stressors. Exposure to an open field/novel object test and associated handling results in higher adrenocortical reactivity than handling alone but the latter (including blood sampling) in itself accounts for proportionately 0·74 of the cortisol response.

Keywords

handlinghydrocortisonepigletsstressweaning
Type
Non-ruminant nutrition, behaviour and production
Information
Animal Science , Volume 74 , Issue 3 , June 2002 , pp. 485 - 491
Copyright
Copyright © British Society of Animal Science 2002

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References

Barnett, J. L., Winfield, C. G., Cronin, G. M., Hemsworth, P. H. and Dewar, A. M. 1985. The effect of individual and group housing on behavioural and physiological responses related to the welfare of pregnant pigs. Applied Animal Behaviour Science 14: 149–161.Google Scholar
Becker, A. B., Ford, J. J., Nienaber, J. A., Hahn, G. L. and Christenson, R. K. 1986. Endocrine and behaviour changes associated with intensive housing systems for swine. In Swine in biomedical research, vol. 1 (ed. Tumbleson, M. E.), pp. 173–189, Plenum Press, New York.Google Scholar
Broom, D. M. 1988. The scientific assessment of animal welfare. Applied Animal Behaviour Science 20: 5–19.Google Scholar
Dantzer, R., Mormède, P., Bluthé, R. M. and Soissons, J. 1983. The effect of different housing conditions on behavioural and adrenocortical reactions in veal calves. Reproduction, Nutrition, Développement 23: 501–508.Google Scholar
Désautés, C., Bidanel, J. -P. and Mormède, P. 1997. Genetic study of behavioral and pituitary-adrenocortical reactivity in response to an environmental challenge in pigs. Physiology and Behavior 62: 337–345.CrossRefGoogle Scholar
Ekkel, E. D., Savenije, B., Schouten, W. G. P., Wiegant, V. M. and Tielen, M. J. M. 1996. The effects of mixing upon behavior, circadian parameters of salivary cortisol and responsiveness to acute stress in pigs. Physiology and Behavior 62: 181–184.Google Scholar
Evans, F. D., Christopherson, R. J. and Aherne, F. X. 1988. Development of the circadian rhythm of cortisol in the gilt from weaning until puberty. Canadian Journal of Animal Science 68: 1105–1111.Google Scholar
Fraser, D. 1978. Observations on the behavioural development of suckling and early-weaned piglets during the first six weeks after birth. Animal Behaviour 26: 22–30.CrossRefGoogle Scholar
Harbuz, M. S. and Lightman, S. L. 1992. Stress and the HPA-axis: acute, chronic and immunological activation. Journal of Endocrinology 134: 327–339.Google Scholar
Herskin, M. S. and Jensen, K. H. 2000. Effects of different degrees of social isolation on the behaviour of weaned piglets kept in metabolism chambers. Animal Welfare 9: 237–249.CrossRefGoogle Scholar
Hessing, M. J. C., Hagelsø, A. M., Schouten, W. G. P., Wiepkema, P. R. and Beek, J. A. M. van. 1994. Individual behavioral and physiolgical strategies in pigs. Physiology and Behavior 55: 39–46.Google Scholar
Janssens, C. J. J. G., Helmond, F. A., Loyens, L. W. S., Schouten, W. G. P. and Wiegant, V. M. 1995. Chronic stress increases the opioid-mediated inhibition of the pituitary-adrenocortical response to acute stress in pigs. Endocrinology 136: 1468–1473.Google Scholar
Jensen, K. H., Oksbjerg, N. and Jørgensen, E. 1994. Dietary salbutamol and level of protein: effects on the acute stress response in pigs. Physiology and Behavior 55: 375–379.Google Scholar
Jensen, K. H., Pedersen, L. J., Giersing Hagelsø, A. M., Heller, K. E., Jørgensen, E. and Ladewig, J. 1995. Intermittent stress in pigs: behavioural and pituitary-adrenocortical reactivity. Acta Agriculturæ Scandinavica 45: 276–285.Google Scholar
Jensen, K. H., Pedersen, L. J., Nielsen, E. K., Heller, K. E., Ladewig, J. and Jørgensen, E. 1996. Intermittent stress in pigs: effects on behaviour, pituitary-adrenocortical axis, growth, and gastric ulceration. Physiology and Behavior 59: 741–748.CrossRefGoogle ScholarPubMed
Jong, I. C. de, Ekkel, E. D., Burgwal, J. A. van de, Lambooij, E., Mechiel Korte, S., Ruis, M. A. W., Koolhaas, J. M. and Blokhuis, H. J. 1998. Effects of strawbedding on physiological responses to stressors and behavior in growing pigs. Physiology and Behavior 64: 303–310.Google Scholar
Jong, I. C. de Prelle, I. T., Burgwal, J. A.van de, Lambooij, E., Mechiel Korte, S., Blokhuis, H. J. and Koolhaas, J. M. 2000. Effects of environmental enrichment on behavioral responses to novelty, learning, and memory, and the circadian rhythm in cortisol in growing pigs. Physiology and Behavior 68: 571–578.Google Scholar
Kanitz, E., Manteuffel, G. and Otten, W. 1998. Effects of weaning and restraint stress on glucocorticoid receptor binding capacity in limbic areas of domestic pigs. Brain Research 804: 311–315.Google Scholar
Kanitz, E., Otten, W., Nürnberg, G. and Brüssow, K. P. 1999. Effects of age and maternal reactivity on the stress response of the pituitary-adrenocortical axis and the sympathetic nervous system in neonatal pigs. Animal Science 68: 519–526.Google Scholar
Kattesh, H. G., Charles, S. F., Baumbach, G. A. and Gillespie, B. E. 1990. Plasma cortisol distribution in the pig from birth to six weeks of age. Biology of the Neonate 58: 220–226.Google Scholar
Klemcke, H. G. 1994. Responses of the porcine pituitary-adrenal axis to chronic intermittent stressor. Domestic Animal Endocrinology 11: 133–149.Google Scholar
Klemcke, H. G. and Pond, W. G. 1991. Porcine adrenal adrenocorticotropic hormone receptors: characterization, changes during neonatal development, and response to a stressor. Endocrinology 128: 2476–2488.Google Scholar
Ladewig, J. and Smidt, D. 1989. Behavior, episodic secretion of cortisol, and adrenocortical reactivity in bulls subjected to tethering. Hormones and Behaviour 23: 344–360.CrossRefGoogle ScholarPubMed
Littell, R. C., Milliken, G. A., Stroup, W. W. and Wolfinger, R. S. 1996. SAS system for mixed models. SAS Institute Inc., Cary, NC.Google Scholar
McCauley, I. and Hartmann, P. E. 1984. Changes in piglet leucocytes, B lymphocytes and plasma cortisol from birth to three weeks after weaning. Research in Veterinary Science 37: 234–241.Google Scholar
McGlone, J. J., Salak, J. L., Lumpkin, E. A., Nicholson, R. I., Gibson, M. and Norman, R. L. 1993. Shipping stress and social status effects on pig performance, plasma cortisol, natural killer cell activity, and leukocyte numbers. Journal of Animal Science 71: 888–896.Google Scholar
Mormède, P., Dantzer, R., Bluthe, R. M. and Caritez, J. C. 1984. Differences in adaptive abilities of three breeds of Chinese pigs. Génétique, Sélection, Évolution 16: 85–102.CrossRefGoogle ScholarPubMed
Munksgaard, L. and Simonsen, H. B. 1996. Behavioral and HPA-axis responses of dairy cows to social isolation and deprivation of lying down. Journal of Animal Science 74: 769–778.Google Scholar
Putten, G.van and Dammers, J. 1976. A comparative study of the well-being of piglets reared conventionally and in cages. Applied Animal Ethology 2: 339–356.Google Scholar
Rushen, J. 1991. Problems associated with the interpretation of physiological data in the assessment of animal welfare. Applied Animal Behaviour Science 28: 381–386.Google Scholar
Worsaae, H. and Schmidt, M. 1980. Plasma cortisol and behaviour in early weaned piglets. Acta Veterinaria Scandinavica 21: 640–657.Google Scholar