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The effects of fish oil supplementation on rumen metabolism and the biohydrogenation of unsaturated fatty acids in beef steers given diets containing sunflower oil

Published online by Cambridge University Press:  09 March 2007

M. R. F. Lee ,
J. K. S. Tweed ,
A. P. Moloney  and
N. D. Scollan
M. R. F. Lee*
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3ED, UK
J. K. S. Tweed
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3ED, UK
A. P. Moloney
Affiliation:
Teagasc, Grange Research Centre, Dunsany, Co. Meath, Republic of Ireland
N. D. Scollan
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3ED, UK
*
†E-mail: [email protected]
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Abstract

Duodenally and ruminally fistulated steers were offered grass silage and one of three concentrates at a ratio of 60: 40 (forage: concentrate on a dry-matter basis) : F0, F1 or F4 at 14 g/kg live weight. The concentrates were designed to be iso-lipid and to provide the same amount of sunflower oil but increasing amounts of fish oil : 0, 1 and 4 g per 100 g, respectively. Ruminal characteristics were measured along with fatty acid intakes and duodenal flows to determine the effect of fish oil on : ruminal pH, ammonia-N concentration, volatile fatty acid (VFA) concentration and polyunsaturated fatty acid (PUFA) metabolism. Fish oil had no significant effect on ruminal pH, ammonia-N concentration or the molar proportions of the major VFA, although total VFA concentration was significantly reduced at the highest level of fish oil inclusion. Fish oil significantly increased the flow of long chain PUFA, total conjugated linoleic acid and vaccenic acid to the duodenum and decreased the flow of stearic acid. Biohydrogenation, as determined by the net loss of fatty acid between the mouth and duodenum, of oleic and linolenic acid was not affected by fish oil inclusion and averaged 0·64 and 0·92, respectively. There was a small increase in the biohydrogenation of linoleic acid with increasing fish oil from 0·89 to 0·92 (P < 0·01) on F0 and F4, respectively. Biohydrogenation of the long chain PUFA C20 : 5(n-3) and C22 : 6(n-3) increased from 0·49 and 0·74 to 0·79 and 0·86 (P < 0·01), respectively when fish oil in the concentrate increased from 1 to 4 g per 100 g. The net effect of fish oil on lipid metabolism appears to inhibit the transition of vaccenic acid to stearic acid in the rumen resulting in a build up of this intermediate in the biohydrogenation pathway of C18 PUFA.

Keywords

fatty acidsfish oilslinoleic acid (conjugated)rumen metabolismvaccenic acid
Type
Research Article
Information
Animal Science , Volume 80 , Issue 3 , June 2005 , pp. 361 - 367
Copyright
Copyright © British Society of Animal Science 2005

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References

Ahnadi, C. E., Beswick, N., Delbecchi, L., Kennelly, J. J. and Lacasse, P. 2002. Addition of fish oil to diets for dairy cows. II. Effects on milk fat and gene expression of mammary lipogenic enzymes. Journal of Dairy Research 69: 521–531.CrossRefGoogle ScholarPubMed
Ashes, J. R., Siebert, B. D., Gulati, S. K., Cuthbertson, A. Z. and Scott, T. W. 1992. Incorporation of n-3 fatty acid of fish oil into tissues and serum lipids of ruminants. Lipids 27: 629–631.CrossRefGoogle ScholarPubMed
Chow, T. T., Fievez, V., Moloney, A. P., Raes, K., Demeyer, D. and Smet, S. de. 2004. Effect of fish oil on in vitro rumen lipolysis, apparent biohydrogenation of linoleic and linolenic acid and accumulation of biohydrogenation intermediates. Animal Feed Science and Technology 117: 1–12.CrossRefGoogle Scholar
Delmonte, P., Roach, J. A. G., Mossoba, M. M., Losi, G. and Yurawecz, M. P. 2004. Synthesis, isolation and GC analysis of all the 6, 8- to 13, 15-cis/trans conjugated linoleic acid isomers. Lipids 39: 185–191.CrossRefGoogle ScholarPubMed
Demirel, G., Wachira, A. M., Sinclair, L. A., Wilkinson, R. G., Wood, J. D. and Enser, M. 2004. Effects of dietary n-3 polyunsaturated fatty acids, breed and dietary vitamin E on the fatty acids of lamb muscle, liver and adipose tissue. British Journal of Nutrition 91: 551–565.CrossRefGoogle ScholarPubMed
Doreau, M. and Chilliard, Y. 1997. Effects of ruminal or postruminal fish oil supplementation on intake and digestion in dairy cows. Reproduction, Nutrition, Development 37: 113–124.CrossRefGoogle ScholarPubMed
Doreau, M. and Ferlay, A. 1994. Digestion and utilisation of fatty acids by ruminants. Animal Feed Science and Technology 45: 379–396.CrossRefGoogle Scholar
Doreau, M., Legay, F. and Bauchart, D. 1991. Effect of source and level of supplemental fat on total and ruminal organic matter and nitrogen digestion in dairy cows. Journal of Dairy Science 74: 2233–2242.CrossRefGoogle ScholarPubMed
Faichney, G. J. 1975. The use of markers to partition digestion within the gastro-intestinal tract of ruminants. In Digestion and metabolism in the ruminant (ed. McDonald, I. W. and Warner, A. C. I.), pp. 277–291. University of New England, Armidale.Google Scholar
Griinari, J. M. and Bauman, D. E. 1999. Biosynthesis of conjugated linoleic acid and its incorportation into meat and milk in rumiants. In Advances in conjugated linoleic acid research, volume 1 (ed. Yurawecz, M. P.,Mossoba, M. M., Kramer, J. K. G., Pariza, M. W. and Nelson, G. J.), pp. 180–200. AOCS Press, Champaign, IL.Google Scholar
Gulati, S. K., Ashes, J. R. and Scott, T. W. 1999. Hydrogenation of eicosapentaenoic and docosahexanenoic acids and their incorporation into milk fat. Animal Feed Science and Technology 79: 57–64.CrossRefGoogle Scholar
Ikwuegbu, O. A. and Sutton, J. D. 1982. The effect of varying the amount of linseed oil supplementation on rumen metabolism in sheep. British Journal of Nutrition 48: 365–375.CrossRefGoogle ScholarPubMed
Jarrett, I. G. 1948. The production of rumen and abomasal fistulae in sheep. Journal of the Council for Scientific and Industrial Research 21: 311–315.Google Scholar
Kemp, P. and Lander, D. J. 1984. Hydrogenation in vitro of α- linolenic acid to stearic acid by mixed cultures of pure strains of rumen bacteria. Journal of General Microbiology 130: 527–533.Google Scholar
Kishino, S., Ogawa, J., Omura, Y., Matsumura, K. and Shimizu, S. 2002. Conjugated linoleic acid production from linoleic acid by lactic acid bacteria. Journal of the American Oil Chemists Society 79: 159–163.CrossRefGoogle Scholar
Kopecny, J., Zorec, M., Mrazek, J., Kobayashi, Y. and Marinsek-Logar, R. 2003. Butyrivibrio hungatei sp. Nov. and Pseudobutyrivibrio xylanivorans sp. nov. , butyrate-producing bacteria from the rumen. International Journal of Systematic and Evolutionary Microbiology 53: 201–209.CrossRefGoogle ScholarPubMed
Kramer, J. K. G. and Zhou, J. 2001. Conjugated linoleic acid and octadecenoic acids: extraction and isolation of lipids. European Journal of Lipid Science and Technology 103: 594–632.3.0.CO;2-R>CrossRefGoogle Scholar
Lee, M. R. F., Harris, L. J., Dewhurst, R. J., Merry, R. J. and Scollan, N. D. 2003. The effect of clover silages on long chain fatty acid rumen transformations and digestion in beef steers. Animal Science 76: 491–501.CrossRefGoogle Scholar
Mosley, E. E., Powell, G. J., Riley, M. B. and Jenkins, T. C. 2002. Microbial biohydrogenation of oleic acid to trans isomers in vitro. Journal of Lipid Research 43: 290–296.CrossRefGoogle ScholarPubMed
Noci, F., O'Kiely, P., Monahan, F. J., Stanton, C. and Moloney, A. P. 2005. Conjugated linoleic acid concentration in m. longissimus dorsi from heifers offered sunflower oil-based concentrates and conserved forages. Meat Science 69: 509–518.CrossRefGoogle Scholar
Oh, D. -K., Hong, G. -H., Lee, Y., Min, S., Sin, H. -S. and Cho, S. K. 2003. Production of conjugated linoleic acid by isolated Bifidobacterium strains. World Journal of Microbiology and Biotechnology 19: 907–912.CrossRefGoogle Scholar
Papadopoulos, G., Goulas, C., Apostolaki, E. and Abril, R. 2002. Effects of dietary supplements of algae, containing polyunsaturated fatty acids, on milk yield and the composition of milk products in dairy ewes. Journal of Dairy Research 69: 357–365.CrossRefGoogle ScholarPubMed
Piperova, L. S., Sampugna, L., Teter, B. B., Kalscheur, K. F., Yurawecz, M. P., Ku, Y., Morehouse, K. M. and Erdman, R. A. 2002. Duodenal and milk trans octadecenoic acid and conjugated linoleic acid (CLA) isomers indicate that postabsorptive synthesis is the predominant CLA in lactating dairy cows. Journal of Nutrition 132: 1235–1241.CrossRefGoogle ScholarPubMed
Rainio, A., Vahvaselka, M. and Laakso, S. 2002. Cell-adhered conjugated linoleic acid regulates isomerisation of linoleic acid by resting cells of Propionibacterium freudenreichii. Applied Microbiology and Biotechnology 60: 481–484.Google ScholarPubMed
Scollan, N. D., Choi, N. J., Kurt, E., Fisher, A. V., Enser, M. and Wood, J. D. 2001a. Manipulating the fatty acid composition of muscle and adipose tissue in beef cattle. British Journal of Nutrition 85: 115–124.CrossRefGoogle ScholarPubMed
Scollan, N. D., Dhanoa, M. S., Choi, N. J., Maeng, W. J., Enser, M. and Wood, J. D. 2001b. Biohydrogenation and digestion of long chain fatty acids in steers fed on different sources of lipid.Journal of Agricultural Science, Cambridge 136: 345–355.CrossRefGoogle Scholar
Shingfield, K. J., Ahvenjärvi, S., Toivonen, V., Ärölä, A., Nurmela, K. V. V., Huhtanen, P. and Griinari, J. M. 2003. Effect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows. Animal Science 77: 165–179.CrossRefGoogle Scholar
Sukhija, P. S. and Palmquist, D. L. 1988. Rapid method for the determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36: 1202–1206.CrossRefGoogle Scholar
Tapiero, H., Nguyen Ba, G., Couvreur, P. and Tew, K. D. 2002. Polyunsaturated fatty acid ( PUFA) and eicosanoids in human health and pathologies. Biomedicine and Pharmacotherapy 56: 215–222.CrossRefGoogle ScholarPubMed
Wachira, A. M., Sinclair, L. A., Wilkinson, R. G., Hallett, K., Enser, M. and Wood, J. D. 2000. Rumen biohydrogenation of n-3 polyunsaturated fatty acids and their effects on microbial efficiency and nutrient digestibility in sheep. Journal of Agricultural Science, Cambridge 135: 419–428.CrossRefGoogle Scholar