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Differential control of lipogenesis and lipolysis during development of ovine preadipocytes in vitro

Published online by Cambridge University Press:  09 March 2007

B. Soret ,
S. E. Melrose ,
E. Finley  and
R. G. Vernon
E. Finley
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
R. G. Vernon
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
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Abstract

The stromovascular fraction of adipose tissue from sheep, like that of other species, contains preadipocytes which can be induced to differentiate in culture, providing a potentially useful system for studying adipocyte development. Differentiation of ruminant preadipocytes has only been partly characterized previously so we have investigated the factors regulating the development of lipogenesis and lipolysis in sheep cells. Insulin, rosiglitazone (a peroxisome proliferation activated receptor-γ agonist) and either dexamethasone or a lipid suplement are required during differentiation for maximum rates of lipogenesis, whereas all four components are required to achieve maximum rates of catecholamine-stimulated lipolysis. Tri-iodothyronine had no effect on the development of lipogenesis but resulted in a reduced rate of catecholamine-stimulated lipolysis. Lipogenesis and lipolysis also differed in that the rate of lipogenesis increased to a maximum at about 10 days of differentiation and then fell, whereas the rate of lipolysis reached a plateau at about 10 days. By contrast to catecholamine-stimulated lipolysis, there is little or no evidence for development of the adenosine-based antilipolytic system; this may be because response to adenosine develops very late during preadipocyte differentiation or additional, unidentified factors are required to induce this antilipolytic system. Lipogenesis in differentiated preadipocytes responded to both insulin and growth hormone. These studies show that the development of lipogenesis and lipolysis are under distinct control systems. Furthermore, while preadipocytes differentiated in vitro show many of the characteristics of adipocytes differentiated in vivo, there are still significant differences.

Keywords

adipocytescell culture, lipogenesislipolysissheep
Type
Research Article
Information
Animal Science , Volume 82 , Issue 4 , August 2006 , pp. 517 - 525
Copyright
Copyright © British Society of Animal Science 2006

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