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A comparison of gas production during incubation with rumen contents in vitro and nylon bag degradability as predictors of the apparent digestibility in vivo and the voluntary intake of hays

Published online by Cambridge University Press:  02 September 2010

K. Khazaal ,
M. T. Dentinho ,
J. M. Ribeiro  and
E. R. Ørskov
K. Khazaal
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
M. T. Dentinho
Affiliation:
Estacao Zootecnica National, Departmento de Nutricao, Santarem, Portugal
J. M. Ribeiro
Affiliation:
Estacao Zootecnica National, Departmento de Nutricao, Santarem, Portugal
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

Ten hays harvested at three stages (early bloom MB, mid bloom MB or in seed) made from lucerne (Medicago sativaj, sweet clover (Melilotus segetalis), Persian clover (Trifolium resupinatum) and pre-bloom (PB) Italian ryegrass (Lolium multiflorum var.), were offered ad libitum to four Merino male sheep and daily intake (g dry matter (DM) per kg M0·75) and DM apparent digestibility (DMD) were measured. In sacco DM degradation (g per 100 g DM), gas production (ml per 200 mg DM), in vitro digestibility and fibre composition (g/kg DM) of the hays were also studied. Gas production or DM degradation were calculated at 6, 12, 24, 48, 72 or 96 h and their kinetics were described using the equation p = a + b(1 - e-ct). Intake and in vivo DMD of the hays were variable (P < 0·01). Lucerne EB and Persian clover (all stages) had the highest nutritive value, whereas sweet clover (all stages) had the lowest. Apart from neutral-detergent fibre, which was only related to intake (r = -0·68; P < 0·05), chemical components and in vitro digestibility were poorly (P > 0·05) related to animal performance. Between 12 and 96 h incubation, intake and in vivo DMD were better related to DM degradation (r = 0·79 to 0·83; r = 0·61 to 0·77) than to gas production (r = 0·73 to 0·80; r = 0·58 to 0·78). Prediction of intake and in vivo DMD from the (a + b) values did not provide a great advantage over using some of the static values of gas production or DM degradation.

Multiple regression using separated kinetics of degradation resulted in highest accuracy for predicting intake and apparent digestibility from gas production (R2 = 0·63; R2 = 0·78) and nylon bag degradability (R2 = 0·77; R2 = 0·89). It was concluded that the gas test has good potentiality as it was capable of predicting not only apparent digestibility, but also intake to a level close to that of the nylon bag technique.

Keywords

harvesting datehayin vitro digestibilityrumen degradationsheep.
Type
Research Article
Information
Animal Production , Volume 57 , Issue 1 , August 1993 , pp. 105 - 112
Copyright
Copyright © British Society of Animal Science 1993

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References

Adebowale, E. A. and Nakashima, Y. 1992. Rumen degradation of some Leguminosae and Graminae roughages: effect of chemical pre-treatment with or without cellulase preparation on dry matter and cell wall disappearance. Animal Feed Science and Technology 38: 219–235.CrossRefGoogle Scholar
Alexander, J. M. and McGowan, N. 1966. A filtration procedure for the in vitro determination of digestibility of herbage. Journal of the British Grassland Society 16:140–147.CrossRefGoogle Scholar
Blummel, M. and Ørskov, E. R. 1993. Comparison of in vitro gas production and nylon bag degradability of roughages in predicting food intake in cattle. Animal Feed Science and Technology 40:109–119.CrossRefGoogle Scholar
Chenost, M., Grenet, E., Demarquilly, C. and Jarrige, R. 1970. The use of the nylon bag technique for the study of forage digestion in the rumen and for predicting feed values. Proceedings of the eleventh international grassland congress, Surfers Paradise, pp. 697–701. University of Queensland Press, St. Lucia.Google Scholar
CIHEAM. 1990. Options méditerranéenes. Table of the nutritive value for ruminants of mediterranean forages and by-products. Serie B. Etudes et recherches no. 4. CIHEAM, EEC.Google Scholar
Coelho, M., Hembry, F. G., Barton, F. E. and Saxton, A. M. 1988. A comparison of microbial, enzymatic, chemical and near-infrared reflectance spectroscopy methods in forage evaluation. Animal Feed Science and Technology 20: 219–231.CrossRefGoogle Scholar
Davidson, J., Mathieson, J. and Boyne, A. W. 1970. The use of automation in determining nitrogen by the Kjeldahl method, with final calculations by computer. Analyst, London 95: 181–193.CrossRefGoogle ScholarPubMed
Ehlke, N. J., Casler, M. D., Drolsom, P. N. and Shenk, J. S. 1986. Divergent selection for in vitro dry matter digestibility in smooth bromegrass. Crop Science 26:1123–1126.CrossRefGoogle Scholar
Ford, C. W. and Elliott, R. 1987. Biodegradability of mature grass cell walls in relation to chemical composition and rumen microbial activity. Journal of Agricultural Science, Cambridge 108: 201–209.CrossRefGoogle Scholar
Hovell, F. D. de B., Nǵambi, J. W. W., Barber, W. P. and Kyle, D. J. 1986. The voluntary intake of hay by sheep in relation to its degradability in the rumen as measured in nylon bags. Animal Production 42:111–118.Google Scholar
Jung, H. G. and Casler, M. D. 1991. Relationship of lignin and esterified phenolics to fermentation of smooth bromegrass fiber. Animal Feed Science and Technology 32:63–68.CrossRefGoogle Scholar
Jung, H. G. and Vogel, K. P. 1992. Lignification of swithshgrass (Panicum virgatum) and big bluestem (Andropogen geradii) plant parts during maturation and its effect on fibre degradability. journal of the Science of Food Agriculture 59:169–176.CrossRefGoogle Scholar
Kawas, J. R., Jorgensen, N. A. and Lu, C. D. 1990. Influence of alfalfa maturity on feed intake and site of nutrient digestion in sheep. Journal of Animal Science 68: 4376–4386.CrossRefGoogle ScholarPubMed
Li, X., Kellaway, R. C, Ison, R. L. and Annison, G. 1992. Chemical composition and nutritive value of mature annual legumes for sheep. Animal Feed Science and Technology 37:221–231.CrossRefGoogle Scholar
Mbwile, R. P. and Udén, P. 1991. Comparison of laboratory methods on precision and accuracy of predicting forage organic matter digestibility. Animal Feed Science and Technology 32: 243–251.CrossRefGoogle Scholar
McDonald, I. 1981. A revised model for the estimation of protein degradability in the rumen. Journal of Agricultural Science, Cambridge 96: 251–252.CrossRefGoogle Scholar
Menke, K. H. and Steingass, H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28: 7–55.Google Scholar
Navaratne, H. V. R. G., Ibrahim, M. N. M. and Shiere, J. B. 1990. Comparison of four techniques for predicting digestibility of tropical feeds. Animal Feed Science and Technology 29:209–221.CrossRefGoogle Scholar
Nordkvist, E. and Aman, P. 1986. Changes during growth in anatomical and chemical composition and in vitro degradability of lucerne. Journal of the Science of Food Agriculture 37: 1–7.CrossRefGoogle Scholar
Ørskov, E. R. 1989. Recent advances in evaluation of roughages as feeds for ruminants. In Advances in animal nutrition (ed. Farell, D. J.), pp. 102–108. University of New England Printery, Armidale.Google Scholar
Orskov, E. R., Reid, G. W. and Kay, M. 1988. Prediction of intake by cattle from degradation characteristics of roughages. Animal Production 46: 29–34.Google Scholar
Ørskov, E. R. and Ryle, M. (ed.) 1990. Energy nutrition in ruminants. Chapter 10.Google Scholar
Piva, G., Santi, E., Belladonna, S. and Curto, O. 1988. Kinetics of in vitro fermentation of forages. Journal of Alimentation and Nutrition of Herbivors 28:101–102.Google Scholar
Statistical Analysis Systems Institute. 1985. Proprietary software release 6.02. SAS user's guide statistic. Cary NC.Google Scholar
Tilley, J. M. A. and Terry, R. A. 1963. A two stage technique for the in vitro digestion of forage crops. Journal of the British Grassland Society 18:104–111.CrossRefGoogle Scholar
Van Soest, P. J. and Wine, R. H. 1967. Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents. Journal of the Association of Official Analytical Chemists 50: 50–55.Google Scholar