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Comparison of enzymatic and mechanical methods for the collection of bovine preantral follicles

Published online by Cambridge University Press:  18 August 2016

S. Saha ,
M. Shimizu ,
M. Geshi  and
Y. Izaike
S. Saha*
Affiliation:
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science, Tsukuba Norindanchi, PO Box 5, Ibaraki 305-0901, Japan
M. Shimizu
Affiliation:
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020-0198, Japan
M. Geshi
Affiliation:
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science, Tsukuba Norindanchi, PO Box 5, Ibaraki 305-0901, Japan
Y. Izaike*
Affiliation:
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science, Tsukuba Norindanchi, PO Box 5, Ibaraki 305-0901, Japan
*
†Present address: Agricultural and Forestry Research Center, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan; E-mail:[email protected]
‡Present address: National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
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Abstract

A comparison was made of various devices to obtain preantral follicles from bovine ovaries. The most productive collection methods in terms of number of follicles obtained were the tissue chopper and the grinder method, with an average number of 122·25 (s.e. 5·06) and 120·45 (s.e. 6·89) preantral follicles, respectively. These were followed by ficoll gradation (119·90, s.e. 7·95), mincer (101·75, s.e. 3·98), cell dissociation sieve (100·50, s.e. 3·42) and homogenizer (95·75, s.e. 6·38). For enzymatic digestion, more time was needed and the method was less productive. Microdissection could supply good quality (80% live when collected), larger sized follicles (120 to 220 µm) but with the lowest yield (10·65, s.e. 0·94) per ovary. The isolated follicles did not show any difference (P > 0·05) in viability until day 7 of in-vitro culture irrespective of method used to harvest follicles. Accordingly the new grinding device can be recommended as a replacement for the existing mechanical devices as it can yield the same percentage (47%) of live preantral follicles but of a wider diameter range (40 to 180 µm) per ovary.

Keywords

Bovidaefollicles
Type
Reproduction
Information
Animal Science , Volume 74 , Issue 1 , February 2002 , pp. 155 - 161
Copyright
Copyright © British Society of Animal Science 2002

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References

Cahill, L.P. 1981. Folliculogenesis in the sheep as influenced by breed, season, and estrous cycle. Journal of Reproduction and Fertility 30: (suppl.) 135–142.Google Scholar
Cavanaugh, D. J., Berndt, W. O. and Smith, T. E. 1963. Dissociation of heart cells by collagenase. Nature 19: 261–262.Google Scholar
Eppig, J. J. 1977. Mouse oocyte development in vitro with various culture systems. Developmental Biology 60: 371–388.Google Scholar
Eppig, J. J. and Downs, S. M. 1987. The effect of hypoxanthine on mouse oocyte growth and development in vitro: maintenance of meiotic arrest and gonadotropin-induced oocyte maturation. Developmental Biology 119: 313–321.Google Scholar
Eppig, J. J. and Schroeder, A. C. 1989. Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro . Biology of Reproduction 41: 268–276.Google Scholar
Erickson, B. H. 1966. Development and senescence of the postnatal bovine ovary. Journal of Animal Science 25: 800–805.Google Scholar
Erickson, B. H., Reynolds, R. A. and Murphree, R. L. 1976. Ovarian characteristics and reproductive performance of aged cows. Biology of Reproduction 15: 555–560.CrossRefGoogle Scholar
Figueiredo, J. R., Hulshof, S. C. J., Hurk, R.van den, Ectors, F. J., Fontes, R. S., Nusgens, B., Bevers, M. M. and Beckers, J. F. 1993. Development of a combined new mechanical and enzymatic method for the isolation of intact preantral follicles from fetal, calf and adult bovine ovaries. Theriogenology 40: 789–799.Google Scholar
Figueiredo, J. R., Hulshof, S. C. J., Hurk, R.van den, Nusgens, B., Bevers, M. M., Ectors, F. J. and Beckers, J. F. 1994. Preservation of oocyte and granulosa cell morphology in bovine preantral follicles cultured in vitro . Theriogenology 41: 1333–1346.CrossRefGoogle ScholarPubMed
Gougeon, A. 1996. Regulation of ovarian follicular development in primates: facts and hypotheses. Endocrine Review 17: 121–154.Google Scholar
Greenwald, G. S. and Moor, R. M. 1989. Isolation and preliminary characterization of pig primordial follicles. Journal of Reproduction and Fertility 87: 561–571.Google Scholar
Grob, H. S. 1964. Enzymatic dissection of the mammalian ovary. Science 146: 73–74.CrossRefGoogle ScholarPubMed
Heller, D., Cahill, D. and Schultz, R. M. 1981. Biochemical studies of mammalian oogenesis: metabolic cooperativity between granulosa cells and growing oocytes. Developmental Biology 84: 455–464.CrossRefGoogle Scholar
Hurk, R.van den, Bevers, M. M. and Beckers, J. F. 1997. In vivo and in vitro development of preantral follicles. Theriogenology 47: 73–82.CrossRefGoogle Scholar
Jewgenow, K. 1998. Role of media, protein and energy supplements on maintenance of morphology and DNA-synthesis of small preantral domestic cat follicles during short-term culture. Theriogenology 49: 1567–1577.Google Scholar
Jewgenow, K. and Goritz, F. 1995. The recovery of preantral follicles from ovaries of domestic cats and their characterization before and after culture. Animal Reproduction Science 39: 285–297.Google Scholar
Jewgenow, K. and Pitra, C. 1993. Hormone-controlled culture of secondary follicle of domestic cats. Theriogenology 39: 527–535.Google Scholar
Katska, L. and Rynska, B. 1998. The isolation and in vitro culture of bovine preantral and early antral follicles of different size classes. Theriogenology 50: 213–222.Google Scholar
Newman Hirshfield, A. 1991. Development of follicles in the mammalian ovary. International Review of Cytology 124: 43–101.Google Scholar
Nicosia, S. V., Evangelista, I. and Batta, S. K. 1976. Rabbit ovarian follicles. I. Isolation technique and characterization at different stages of development. Biology of Reproduction 13: 423–447.Google Scholar
Nuttinck, F., Mermillod, P., Massip, A. and Dessy, F. 1993. Characterization of in vitro growth of bovine preantral ovarian follicles: a preliminary study. Theriogenology 39: 811–821.Google Scholar
Peters, H. 1976. The development and maturation of the ovary. Annales de Biologie Animale, Biochimie et Biophysique 16: 271–278.Google Scholar
Poste, G. 1971. Absorption and activity of enzymes at the cell surface. Tissue dissociation with proteolytic enzymes. Experimental Cell Research 65: 359–367.CrossRefGoogle Scholar
Ralph, J. H., Wilmut, I. and Telfer, E. E. 1995. In vitro growth of bovine preantral follicles and the influence of FSH on follicular and oocyte diameters. Journal of Reproduction and Fertility 15: 6 (abstr.).Google Scholar
Roy, S. K. and Greenwald, G. S. 1985. An enzymatic method for dissociation of intact follicles form the hamster ovary: histological and quantitative aspects. Biology of Reproduction 32: 203–215.Google Scholar
Roy, S. K. and Treacy, B. J. 1993. Isolation and long-term culture of human preantral follicles. Fertility and Sterility 59: 783–790.Google Scholar
Russe, I. 1983. Oogenesis in cattle and sheep. Bibliographie Anatomique 24: 77–92.Google Scholar
Statistical Analysis Systems Institute. 1988. SAS/STAT user’s guide, version 6, fourth edition. SAS Institute, Cary, NC.Google Scholar
Telfer, E., Torrance, C. and Gosden, R. G. 1990. Morphological study of cultured preantral follicles of mice after transplantation under kidney capsule. Journal of Reproduction and Fertility 89: 565–571.CrossRefGoogle ScholarPubMed
Telfer, E. E. 1996. The development of methods for isolation and culture of preantral follicles from bovine and porcine ovaries. Theriogenology 45: 101–110.Google Scholar
Torrance, C., Telfer, E. and Gosden, R. G. 1989. Quantitative study of the development of isolated mouse pre-antral follicle in collagen gel culture. Journal of Reproduction and Fertility 87: 367–374.Google Scholar
Wandji, S. A., Eppig, J. J. and Fortune, J. E. 1996. FSH and growth factors affect the growth and endocrine function in vitro of granulosa cells of bovine preantral follicles. Theriogenology 45: 817–832.CrossRefGoogle ScholarPubMed