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Assessment of blood neutrophil oxidative burst activity in dairy cows during the period of parturition

Published online by Cambridge University Press:  02 September 2010

F. Moreira da Silva ,
C. Burvenich ,
A. M. Massart Leën  and
L. Brossé
F. Moreira da Silva
Affiliation:
University of Gent, Faculty of Veterinary Medicine, Department of Physiology, Biochemistry and Biometrics, Salisburylaan, 133-B-9820 Merelbeke-Belgium
C. Burvenich
Affiliation:
University of Gent, Faculty of Veterinary Medicine, Department of Physiology, Biochemistry and Biometrics, Salisburylaan, 133-B-9820 Merelbeke-Belgium
A. M. Massart Leën
Affiliation:
University of Gent, Faculty of Veterinary Medicine, Department of Physiology, Biochemistry and Biometrics, Salisburylaan, 133-B-9820 Merelbeke-Belgium
L. Brossé
Affiliation:
University of Gent, Faculty of Veterinary Medicine, Department of Physiology, Biochemistry and Biometrics, Salisburylaan, 133-B-9820 Merelbeke-Belgium
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Abstract

Bovine blood neutrophil oxidative burst activity, the total leukocyte count and its differentiation, as well as progesterone, glucose and cortisol levels were studied on 10 cows from 1 week prior to calving until 8 weeks after parturition.

From day –7 before parturition a decrease in the metabolic oxidative burst activity of neutrophils was observed, reaching the lowest value 3 days after calving (0·28 (s.e. 0·06) nmol/l H2O2 per min 106 neutrophils) (P <0·01). Thereafter, values increased with a maximum of activity 26 days after parturition (0·57 (s.e. 0·05) nmol/H202 per min per 106 neutrophils). At the end of the experiment the neutrophils H2O2-productionwas comparable to values observed on day–7.

The maximum total leukocyte count was observed on the day of parturition (8, 108 (s.e. 370) leukocytes per /A blood) (P <0·01) returning to normal levels about 3 weeks after calving. Lymphocytes and neutrophils showed a trend analogous to that of the total leukocytes. Eosinophils and monocytes did not change during the entire period. With the rapid increase of circulating neutrophils observed on the calving day, a marked left shift was observed with a maximum number of immature neutrophils (48·6%) observed on day +3 (1477 (s.e. 242) immature neutrophils per fjl blood) (P <0·01), reaching normal values about 4 weeks after parturition.

A significant increase (F < 0·01) of plasma glucose was found on the day of parturition (91·4 (s.e. 6·8) mg per 100 ml plasma), while the levels of cortisol reached their maximum 3 days after calving (22·05 (s.e. 8·46) nglml plasma).

Progesterone levels averaged 4·07 (s.e. 1·21) jigll 7 days before calving, declining precipitously to 0·06 (s.e. 0·45) Hg/l 3 days after calving (F < 0·01), remaining very low for a period of about 3 weeks. Minimum and maximum anoestrus post-partum were 22 and 57 days, respectively.

Despite great hormonal variation observed in the peripartum period, no significant correlation was found between measured hormones and the oxidative burst activity of neutrophils.

Keywords

cowsneutrophilsparturition
Type
Research Article
Information
Animal Science , Volume 67 , Issue 3 , December 1998 , pp. 421 - 426
Copyright
Copyright © British Society of Animal Science 1998

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References

Bodel, P., Dillard, G. M., Kaplan, S. S. and Malawista, S. E. 1972. Anti-inflammatory effects of estradiol on human blood leukocytes. Journal of Laboratory and Clinical Medicine. 80: 373–384.Google ScholarPubMed
Brevers, G., Harmeyer, J., Fames, E. and Hoeller, H. 1980. Glucocorticoid levels in blood plasma of normal and ketotic cows. Journal of Animal Science. 50: 503–507.CrossRefGoogle Scholar
Burvenich, C., Guidry, A. J. and Paape, M. J. 1995. Natural defence mechanisms of the lactating and dry mammary gland Proceedings of the third international mastitis seminar, Tel Aviv, Israel, vol. 1, pp. 3–13.Google Scholar
Comline, R. S., Hall, L. W., Lavelle, R. B., Nathanielsz, P. W. and Silver, M. 1974. Parturition in the cow: endocrine changes in animals with chronically implanted catheters in the foetal and maternal circulations. Journal of Endocrinology. 63: 451–472.CrossRefGoogle ScholarPubMed
Concannon, P. W., Butler, W. R., Hansel, W., Knight, P. J. and Hamilton, J. M. 1978. Parturition and lactation in the bitch: serum progesterone, cortisol and prolactin. Biology of Reproduction. 19: 1113–1118.CrossRefGoogle ScholarPubMed
Curtis, C. R., Erb, H. N., Sniffen, C. J., Smith, R. D., Powers, P. A., Smith, M. C., White, M. E., Hillian, R. B. and Pearson, E. J. 1983. Association of parturient hypocalcemia with eight periparturient disorders in Holstein cows. Journal of the American Veterinary Medical Association. 183: 559–561.Google ScholarPubMed
Glasser, L. and Fiederlein, R. L. 1987. Functional differentiation of normal human neutrophils. Blood. 69: 937–944.CrossRefGoogle ScholarPubMed
Guidry, A. J., Faape, M. J. and Pearson, R. E. 1976. Effects of parturition and lactation on blood and milk cell concentrations, corticosteroids and neutrophil phagocytose n i the cow. American Journal of Veterinary Research. 37: 1195–1200.Google Scholar
Haris, R. D., Kay, N. E., Seljeskob, E. L., Murry, K. J. and Douglas, S. D. 1979. Prolactin suppression of leukocyte chemotaxis in vitro. Journal of Neurosurgery. 50: 462–465.CrossRefGoogle Scholar
Heyneman, R., Burvenich, C. and Vercauteren, R. 1990. Interaction between the respiratory burst activity of neutrophil leukocytes and experimentally induced Escherichia coli mastitis in cows. Journal of Dairy Science 73: 985–994.CrossRefGoogle ScholarPubMed
Hill, A. W., Shears, A. L. and Hibbit, K. G. 1979. The pathogenesis of experimental Escherichia coli mastitis in newly calved dairy cows. Research in Veterinary Science. 26: 97–101.CrossRefGoogle ScholarPubMed
Huggett, A. S. T. G. and Nixon, D. A. 1957. Enzymatic determination of blood glucose. Biochemical Journal 68: 12.Google Scholar
Kehrli, M. E. Jr, Nonnecke, B. J. and Roth, J. A. 1989. Alterations in bovine neutrophil function during the periparturient period. American Journal of Veterinary Research 50: 207–214.Google ScholarPubMed
Klebanoff, S. J. 1979. Effect of estrogens on the myeloperoxidase-mediated antimicrobial system. Infection and Immunity. 25: 152–156.CrossRefGoogle ScholarPubMed
Lovgren, T., Hemmila, I., Pettersson, K., Eskola, J. V. and Betorf, E. 1984. Determination of hormones by time-resolved fluoroimmunoassay. Talanta. 31: 909–916.CrossRefGoogle ScholarPubMed
McDonald, J. S. and Anderson, A. J. 1981. Experimental intramammary infection of the dairy cow with Escherichia coli during the nonlactating period. American Journal of Veterinary Research 42: 229–231.Google ScholarPubMed
Moreira da Silva, F. and Burvenich, C. 1996. In vitro effect of progesterone, estradiol 17 β, FSH, LH and GnRH on the oxidative burst activity of polymorphonuclear leukocytes from cows. Revista Portuguesa de Zootecnia In press.Google Scholar
Newbold, F. H. S. 1976. Phagocytic activity of bovine leukocytes during pregnancy. Canadian Journal of Comparative Medicine. 40: 111–116.Google Scholar
Paape, M. J., Gwazdauskas, F. C., Guidry, A. J. and Weinland, B. T. 1981. Concentrations of corticosteroids, leukocytes and immunoglobulins in blood and milk after administration of ACTH to lactating dairy cattle: effects on phagocytosis of Staphylococus aureus by polymorphonuclear leukocytes. American Journal of Veterinary Research. 42: 2081–2087.Google Scholar
Reilli, P. E. B. and Black, A. 1973. Early effects of cortisol on glucose and alanine metabolism in adrenalectomized sheep. American Journal of Physiology. 225: 689–695.CrossRefGoogle Scholar
Robinson, N. A., Leslie, K. E. and Walton, J. S. 1989. Effect of treatment with progesterone on pregnancy rate and plasma concentrations of progesterone in Holstein cows. Journal of Dairy Science 72: 202–207.CrossRefGoogle ScholarPubMed
Root, R. K., Metcalf, J., Oshino, N. and Chance, B. 1975. H2O2 release from human granulocytes during phagocytosis. Journal of Clinical Investigation. 55: 945–955.CrossRefGoogle ScholarPubMed
Roth, J. A., Kaeberle, M. L., Appell, L. H. and Nachreiner, R. F. 1983a. Association of increased estradiol and progesterone blood values with altered bovine polymorphonuclear leukocyte function. American Journal Veterinary Research. 44: 247–253.Google ScholarPubMed
Roth, J. A., Kaeberle, M. L. and Hsu, W. H. 1983b. Effect of estradiol and progesterone on lymphocyte and neutrophil functions in steers. Infection and Immunity. 35: 997–1002.CrossRefGoogle Scholar
Saad, A. M., Concha, C. and Astrom, G. 1989. Alterations i n neutrophil phagocytosis and lymphocyte blastogenesis i n dairy cows around parturition. Journal of Veterinary Medicine, Series B. 36: 337–345.CrossRefGoogle Scholar
Schalm, O. W. 1962. Leukocyte responses to disease in various domestic animals. Journal of the American Veterinary Medical Association. 140: 557–563.Google ScholarPubMed
Silva, D. A., Jain, N. C. and George, L. W. 1989. Phagocytic and nitroblue tetrazolium reductive properties of mature and immature neutrophils and eosinophils from blood and bone marrow from cows. American Journal of Veterinary Research. 50: 778–781.Google ScholarPubMed
Smith, V. G., Edgerton, L. A., Hafs, H. D. and Convey, E. M. 1973. Bovine serum estrogens, progestins and glucocorticosteroids during late pregnancy, parturition and early lactation. Journal of Animal Science. 36: 391–396.CrossRefGoogle Scholar
Sokal, R. R. and Rohlf, F. J. 1981. Single classification analysis of variance: biometry, p. 208. W. H. Freeman Company, New York.Google Scholar