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16-androstene steroids in the submaxillary salivary gland of the boar in relation to measures of boar taint in carcasses

Published online by Cambridge University Press:  02 September 2010

W. D. Booth ,
E. Diane Williamson  and
R. L. S. Patterson
W. D. Booth
Affiliation:
AFRC Institute of Animal Physiology, Animal Research Station, 307 Huntingdon Road, Cambridge CB3 0JQ
E. Diane Williamson
Affiliation:
AFRC Food Research Institute, Langford, Bristol BS18 7DY
R. L. S. Patterson
Affiliation:
AFRC Food Research Institute, Langford, Bristol BS18 7DY
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Abstract

Fifteen Large White boars were randomly allocated to three groups of five pigs. The boars were slaughtered over a period of time at one of three live weights: group 1 (pork weight), 63 to 68 kg (mean age 141 days), group 2 (bacon weight), 90 to 97 kg (mean age 203 days) and group 3 (heavy weight), 121 to 133 kg (mean age 268 days). Testes, bulbourethral glands, submaxillary salivary glands, blood and thoracic fat were taken from each pig and the glands weighed and steroids determined in extracts of the submaxillary glands, blood plasma and fat. Testosterone and the boar taint steroid, 5a-androstenone (5a-androst-16-en-3-one) were measured in plasma by radioimmunoassay (RIA). 3a-androstenol (5a-androst-16-en-3a-ol) and 5a-androstenone were determined separately in submaxillary glands by gas-liquid chromatography and together semi-quantitatively by a colour reaction. 5a-androstenone was also determined in submaxillary glands by RIA and in fat by enzyme-linked immunosorbent assay. Mean somatic characteristics differed significantly between the groups, but due to the wide variation in steroid concentrations between individual boars, mean differences for steroid concentrations between groups were not significant.

Significant positive correlations were found in boars between bulbourethral gland weight, submaxillary gland weight, concentrations of 3a-androstenol and 5a-androstenone in the submaxillary gland and concentrations of free plasma 5a-androstenone and fat 5a-androstenone (P < 0·05). The application of a simple colour reaction to detect readily extractable 16-androstene steroids (16-androstenes) in submaxillary gland tissue is discussed in relation to other methods for determining boar taint.

Type
Research Article
Information
Animal Production , Volume 42 , Issue 1 , February 1986 , pp. 145 - 152
Copyright
Copyright © British Society of Animal Science 1986

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References

REFERENCES

Andresen, Ø. 1974. Development of a radio-immunoassay for 5a-androst-16-en-3-one in pig peripheral plasma. Ada endocr., Copnh. 76: 377–387.Google Scholar
Andresen, Ø. 1975. A radioimmunoassay for 5a-androst-16-en-3-one in porcine adipose tissue. Acta endocr., Copnh. 79: 619–624.Google Scholar
Andresen, Ø. 1976. Concentrations of fat and plasma 5a-androstenone and plasma testosterone in boars selected for rate of body weight gain and thickness of back fat during growth, sexual maturation and after mating. J. Reprod. Fert. 48: 51–59.CrossRefGoogle ScholarPubMed
Baxendale, P. M., Jacobs, H. S. and James, V. H. T. 1982. Salivary testosterone; relationship to unbound plasma testosterone in normal and hyperandrogenic women. Clin. Endocr. 16: 595–603.CrossRefGoogle ScholarPubMed
Bonneau, M. 1982. Compounds responsible for boar taint with special emphasis on androstenone: a review. Livest. Prod. Sci. 9: 687–705.CrossRefGoogle Scholar
Bonneau, M. and Desmoulin, B. 1981. Influence de I'excès de tryptophane et des conditions d'elevages sur la fréquence des odeurs sexuelles des jeunes pores mâles entiers: relation avec le développement de l'appareil génital. J. Rech. Porcine France 13: 329–334.Google Scholar
Booth, W. D. 1975. Changes with age in the occurrence of C,9 steroids in the testis and submaxillary gland of the boar. J. Reprod. Fert. 42: 459–472.CrossRefGoogle Scholar
Booth, W. D. 1982. Testicular steroids and boar taint. In Control of Pig Reproduction (ed. Cole, D. J. A. and Foxcroft, G. R., pp. 25–48. Butterworth, London.CrossRefGoogle Scholar
Booth, W. D. 1984. Sexual dimorphism involving steroidal pheromones and their binding protein in the submaxillary salivary gland of the Göttingen miniature pig. J. Endocr. 100: 195–202.CrossRefGoogle ScholarPubMed
Booth, W. D. and Baldwin, B. A. 1980. Lack of effect on sexual behaviour or the development of testicular function after removal of olfactory bulbs in prepubertal boars. J. Reprod. Fert. 58: 173–182.CrossRefGoogle ScholarPubMed
Brooksbank, B. W. L. and Hazlewood, G. A. D. 1961. The estimation of androst-16-en-3α-ol in human urine. Biochem, J. 80: 488–496.CrossRefGoogle ScholarPubMed
Claus, R. 1979. The EAAP working group on production and utilisation of meat from entire male pigs: report on 1977 meeting. Livest. Prod. Sci. 6: 319–321.Google Scholar
Desmoulin, B. and Rhodes, D. N. 1975. The EAAP working group on production and utilisation of meat from entire male pigs: report on 1975 meetings. Livest. Prod. Sci. 2: 317–324.Google Scholar
Forland, D. M., Lundstro, M. K. and Andresen, O. 1980. Relationship between androstenone content in fat, intensity of boar taint and size of accessory sex glands in boars. Nord. VetMed. 32: 201–206.Google ScholarPubMed
Jarmoluk, L., Martin, A. H. and Fredeen, H. T. 1970. Detection of taint (sex odour) in pork. Can. J. Anim. Sci. 50: 750–752.CrossRefGoogle Scholar
Malmfors, B. and Hansson, I. 1974. Incidence of boar taint in Swedish Landrace and Yorkshire boars. Livest. Prod. Sci. 1: 411–420.CrossRefGoogle Scholar
Melrose, D. R., Reed, H. C. B. and Patterson, R. L. S. 1971. Androgen steroids associated with boar odour as an aid to the detection of oestrus in pig artificial insemination. Br. vet. J. 127: 497–502.CrossRefGoogle Scholar
Patterson, R. L. S. 1968a. Identification of 3α-hydroxy-5a-androst-16-ene as the musk odour component of boar submaxillary salivary gland and its relationship to the sex odour taint in pork meat. J. Sci. Fd Agric. 19: 434–438.CrossRefGoogle Scholar
Patterson, R. L. S. 1968b. 5a-androst-16-ene-3-one:—compound responsible for taint in boar fat. J. Sci. Fd Agric 19: 31–38.CrossRefGoogle Scholar
Patterson, R. L. S. and Lightfoot, A. L. 1984. Effect of sex grouping during growth on 5α-androstenone development in boars at three commercial slaughter weights. Meat Sci. 10: 253–263.CrossRefGoogle ScholarPubMed
Perry, G. C., Patterson, R. L. S., MacFie, H. J. H. and Stinson, C. G. 1980. Pig courtship behaviour: pheromonal property of androstene steroids in male submaxillary secretion. Anim. Prod. 31: 191–199.Google Scholar
Uzu, G. and Bonneau, M. 1980. Relationships between sperm production and androstenone level in boar tissue. Annls Zootech. 29: 23–30.CrossRefGoogle Scholar
Williamson, E. Diane and Patterson, R. L. S. 1982. A selective immunization procedure against 5a-androstenone in boars. Anim. Prod. 35: 353–360.Google Scholar
Williamson, E. Diane, Patterson, R. L. S., Buxton, E. R., Mitchell, K. G., Partridge, I. G. and Walker, N. 1985. Immunization against 5a-androstenone in boars. Livest. Prod. Sci. 12: 251–264.CrossRefGoogle Scholar