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The use of culture, pooled samples and PCR for identification of herds infected with Brachyspira hyodysenteriae

Published online by Cambridge University Press:  28 February 2007

Claes Fellström ,
Ulla Zimmerman ,
Anna Aspan  and
Anders Gunnarsson
Claes Fellström*
Affiliation:
Department of Large Animal Clinical Sciences, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Uppsala, Sweden
Ulla Zimmerman
Affiliation:
Department of Bacteriology, National Veterinary Institute, Uppsala, Sweden
Anna Aspan
Affiliation:
Department of Bacteriology, National Veterinary Institute, Uppsala, Sweden
Anders Gunnarsson
Affiliation:
Department of Bacteriology, National Veterinary Institute, Uppsala, Sweden
*
*Department of Large Animal Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7018, SE-750 07 Uppsala, Sweden. E-mail: [email protected]
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Abstract

The sensitivity of culturing Brachyspira hyodysenteriae was determined after sampling with swabs from porcine fecal specimens inoculated with tenfold dilutions of a field strain of these microbes. After storage of swabs, Brachyspira hyodysenteriaewas recovered throughout the first 3 weeks after inoculation from feces with more than 140 cells/g. Viable spirochetes could still be recovered after up to 83 days of storage from feces, with 1.4 × 106 cells or more per gram. Culture for Brachyspira spp. was performed on 285 rectal swabs, which were pooled in batches of five. The number of pooled samples positive for B. hyodysenteriae corresponded with the sum results of individual analysis of the corresponding collections of five samples. A PCR system based on the tlyA gene of B. hyodysenteriae was developed and tested on primary cultures of pooled samples. The results of the PCR assay showed a 97% correlation with the culture results. The prevalence of Brachyspira spp. was determined in five swine herds and found to be highest among breeding gilts and boars aged 13–16 weeks and among 6–12-week-old weaned pigs. In contrast, Brachyspiraspp. were only rarely found in sows, which may reflect the development of immunity by adult pigs to all species of the genus.

Type
Research Article
Information
Animal Health Research Reviews , Volume 2 , Issue 1 , June 2001 , pp. 37 - 43
Copyright
Copyright © CAB International 2001

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