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Ultraviolet irradiation and the mechanisms underlying its inactivation of infectious agents

Published online by Cambridge University Press:  15 June 2011

Timothy D. Cutler  and
Jeffrey J. Zimmerman
Timothy D. Cutler*
Affiliation:
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, IA 50011-1250, USA
Jeffrey J. Zimmerman
Affiliation:
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, IA 50011-1250, USA
*
*Corresponding author. E-mail: [email protected]
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Abstract

We review the principles of ultraviolet (UV) irradiation, the inactivation of infectious agents by UV, and current applications for the control of microorganisms. In particular, wavelengths between 200 and 280 nm (germicidal UV) affect the double-bond stability of adjacent carbon atoms in molecules including pyrimidines, purines and flavin. Thus, UV inactivation of microorganisms results from the formation of dimers in RNA (uracil and cytosine) and DNA (thymine and cytosine). The classic application of UV irradiation is the inactivation of microorganisms in biological safety cabinets. In the food-processing industry, germicidal UV irradiation has shown potential for the surface disinfection of fresh-cut fruit and vegetables. UV treatment of water (potable and wastewater) is increasingly common because the process is effective against a wide range of microorganisms, overdose is not possible, chemical residues or by-products are avoided, and water quality is unaffected. UV has been used to reduce the concentration of airborne microorganisms in limited studies, but the technology will require further development if it is to gain wider application. For bioaerosols, the primary technical challenge is delivery of sufficient UV irradiation to large volumes of air, but the absence of UV inactivation constants for airborne pathogens under a range of environmental conditions (temperature, relative humidity) further compounds the problem.

Keywords

UltravioletUVUV irradiationUVCgermicidal UVMicrobial InactivationInactivation
Type
Review Article
Information
Animal Health Research Reviews , Volume 12 , Issue 1 , June 2011 , pp. 15 - 23
Copyright
Copyright © Cambridge University Press 2011

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References

Alexanderson, S, Brotherhood, I and Donaldson, AI (2002). Natural aerosol transmission of foot-and-mouth disease virus to pigs: minimal infectious dose for strain O1 Luasanne. Epidemiology and Infection 128: 301–312.Google Scholar
Artés-Hernéndez, F, Escalona, VH, Robles, PA, Martínez-Hernández, GB and Artés, F (2009). Effect of UV-C radiations on quality of minimally processed spinach leaves. Journal of the Science of Food and Agriculture 89: 414–421.Google Scholar
Barnard, J and Morgan, H (1903). The physical factors in phototherapy. British Medical Journal 2: 1269–1271.Google Scholar
Berg, M, Bergman, BR and Hoborn, J (1991). Ultraviolet radiation compared to an ultra-clean air enclosure. Journal of Bone and Joint Surgery (British) 73: 811–815.Google Scholar
Blachere, FM, Lindsley, WG, Slaven, JE, Green, BJ, Anderson, SE, Chen, BT and Beezhold, DH (2007). Bioaerosol sampling for the detection of aerosolized influenza virus. Influenza and Other Respiratory Viruses 1: 113–120.CrossRefGoogle ScholarPubMed
Bolton, JR (2000). Calculation of ultraviolet fluence rate distributions in an annular reactor: significance of refraction and reflection. Water Research 34: 3315–3324.Google Scholar
Bolton, JR and Linden, KG (2003). Standardization of methods for fluence (UV dose) determination in bench-scale UV experiments. Journal of Environmental Engineering 129: 209–213.CrossRefGoogle Scholar
Centers for Disease Control and Prevention, (CDC) (2009). Appendix A – Primary containment for biohazards: selection, installation and use of biological safety cabinets. In: Chosewood, L Casey and Deborah, E (eds) Biosafety in Microbiological and Biomedical Laboratories, 5th ed. Wilson: HHS Publication No. (CDC), pp. 21–112.Google Scholar
Cox, CS (1976). Inactivation kinetics of some microorganisms subjected to a variety of stresses. Applied and Environmental Microbiology 31: 836–846.Google Scholar
Douwes, J, Thorne, P, Pearce, N and Heederik, D (2003). Bioaerosol health effects and exposure assessment: progress and prospects. Annals of Occupational Hygiene 47: 187–200.Google ScholarPubMed
Eischeid, AC, Meyer, JN and Linden, KG (2009). UV disinfection of adenoviruses: molecular indications of DNA damage efficiency. Applied and Environmental Microbiology 75: 23–28.Google Scholar
Environmental Protection Agency (2003). Ultraviolet Disinfection Guidance Manual. EPA 815-D-03-007, June 2003. Washington, DC: EPA.Google Scholar
Environmental Protection Agency (2006a). Biological inactivation efficiency by HVAC in-duct ultraviolet light systems. Novatron, Inc. BioProtector BP114i. September 2006. EPA 600/R-06/084.Google Scholar
Environmental Protection Agency (2006b). Biological inactivation efficiency by HVAC in-duct ultraviolet light systems. American Ultraviolet Corporation ACP-24/HO-4. May 2006. EPA 600/R-06/054.Google Scholar
Environmental Protection Agency (2006c). Biological inactivation efficiency by HVAC in-duct ultraviolet light systems. Lumalier. June 2006. EPA 600/R-06/055.Google Scholar
Environmental Protection Agency (2006d). Biological inactivation efficiency by HVAC in-duct ultraviolet light systems. UltraViolet Devices, Inc. Altru-V V-Flex. May 2006. EPA 600/R-06/049.Google Scholar
Escombe, AR, Oeser, CC, Gilman, RH, Navincopa, M, Ticona, E, Pan, W, Martínez, C, Chacaltana, J, Rodríguez, R, Moore, DA, Friedland, JS and Evans, CA (2007). Natural ventilation for the prevention of airborne contagion. PLoS Medicine 4: e68.CrossRefGoogle ScholarPubMed
Goodsell, DS (2001). The molecular perspective: ultraviolet light and pyrimidine dimers. The Oncologist 6: 298–299.CrossRefGoogle ScholarPubMed
Guerrero-Beltrán, JA and Barbosa-Cánovas, BV (2005). Reduction of Saccharomyces cerevisiae, Escherichia coli and Listeria innocua in apple juice by ultraviolet light. Journal of Food Processing and Engineering 28: 437–452.CrossRefGoogle Scholar
Hermann, JR, Muñoz-Zanzi, CA and Zimmerman, JJ (2009). A method to provide improved dose-response estimates for airborne pathogens in animals: an example using porcine reproductive and respiratory syndrome virus. Veterinary Microbiology 133: 297–302.CrossRefGoogle ScholarPubMed
Hiatt, CW (1964). Kinetics of the inactivation of viruses. Bacteriological Reviews 28: 150–163.Google Scholar
Hijnen, WAM, Beerendonk, EF and Medema, GJ (2006). Inactivation credit of UV radiation for viruses, bacteria and protozoan (oo)cysts in water: a review. Water Research 40: 3–22.CrossRefGoogle ScholarPubMed
Jagger, J (1967). Introduction to Research in Ultraviolet Photobiology. Englewood Cliffs, NJ: Prentice-Hall, Inc.Google Scholar
Kleczkowski, A (1963). The inactivation of ribonucleic acid from tobacco mosaic virus by ultraviolet radiation at different wavelengths. Photochemistry and Photobiology 2: 497–503.Google Scholar
Ko, G, First, MW and Burge, HA (2000). Influence of relative humidity on particle size and UV sensitivity of Serratia marcescens and Mycobacterium bovis BCG aerosols. Tubercle and Lung Disease 80: 217–228.CrossRefGoogle ScholarPubMed
Kowalski, W (2009). Ultraviolet Germicidal Irradiation Handbook: UVGI for Air and Surface Disinfection. New York: Springer.CrossRefGoogle Scholar
Kowalski, WJ, Bahnfleth, WP and Carey, DD (2002). Engineering control of airborne disease transmission in animal laboratories. Contemporary Topics in Laboratory Animal Science 41: 9–17.Google Scholar
Kuhn, HJ, Braslavsky, SE and Schmidt, R (2004). Chemical actinometry. Pure and Applied Chemistry 76: 2105–2146.CrossRefGoogle Scholar
Lai, KM, Burge, HA and First, MW (2004). Size and UV germicidal irradiation susceptibility of Serratia marcescens when aerosolized from different suspending media. Applied and Environmental Microbiology 70: 2021–2027.CrossRefGoogle ScholarPubMed
Levetin, E, Shaughnessy, R, Rogers, CA and Scheir, R (2001). Effectiveness of germicidal UV radiation for reducing fungal contamination within air-handling units. Applied and Environmental Microbiology 67: 3712–3715.CrossRefGoogle ScholarPubMed
Linden, KG, Thurston, J, Schaefer, R and Malley, JP (2007). Enhanced UV inactivation of adenoviruses under polychromatic UV lamps. Applied and Environmental Microbiology 73: 7571–7574.Google Scholar
Meechan, PJ and Wilson, C (2006). Use of ultraviolet lights in biological safety cabinets: a contrarian view. Applied Biosafety 11: 222–227.Google Scholar
Memarzadeh, F, Olmsted, RN and Bartley, JM (2010). Applications of ultraviolet germicidal irradiation disinfection in health care facilities: effective adjunct, but not stand-alone technology. American Journal of Infection Control 38: 13–24.Google Scholar
Menetrez, MY, Foarde, KK, Dean, TR and Betancourt, DA (2010). The effectiveness of UV irradiation on vegetative bacteria and fungi surface contamination. Chemical Engineering Journal 157: 443–450.Google Scholar
Menzies, D, Pasztor, J, Rand, T and Bourbeau, J (1999). Germicidal ultraviolet irradiation in air conditioning systems: effect on office worker health and wellbeing – a pilot study. Occupational and Environmental Medicine 56: 397–402.Google Scholar
Mercier, J, Arul, J, Ponnampalamm, R and Boulet, M (1993). Induction of 6-methoxymellein and resistance to storage pathogens in carrot slices by UV-C. Journal of Phytopathology 137: 44–54.Google Scholar
Miller, RL and Plagemann, PGW (1974). Effect of ultraviolet light on mengovirus: formation of uracil dimers, instability and degradation of capsid and covalent linkage of protein to viral RNA. Journal of Virology 13: 729–739.CrossRefGoogle ScholarPubMed
Nigro, F, Ippolito, A and Lima, G (1998). Use of UV-C light to reduce Botrytis storage rot of table grapes. Postharvest Biology and Technology 13: 171–181.CrossRefGoogle Scholar
O'Donnell, R, Boorstein, ERJ, Cunningham, RP and Teebor, GW (1994). Effect of pH and temperature on the stability of UV-induced repairable pyrimidine hydrates in DNA. Biochemistry 33: 9875–9880.Google Scholar
Peccia, J, Werth, HM, Miller, S and Hernadez, M (2001). Effect of relative humidity on the ultraviolet-induced inactivation of airborne bacteria. Aerosol Science and Technology 35: 728–740.Google Scholar
Perkins, JE, Bahlke, AM and Silverman, HF (1947). Effect of ultra-violet irradiation of classroom on the spread of measles in large rural central schools. American Journal of Public Health 37: 529–537.Google Scholar
Qualls, RG and Johnson, JD (1983). Bioassay and dose measurement in UV disinfection. Applied and Environmental Microbiology 45: 872–877.CrossRefGoogle ScholarPubMed
Rahn, RO, Bolton, R and Stefan, MI (2005). The iodide/iodate actinometer in UV disinfection: determination of the fluence rate distribution in UV reactors. Photochemistry and Photobiology 82: 611–615.Google Scholar
Riley, RL (1961). Airborne pulmonary tuberculosis. Bacteriological Reviews 25: 243–248.Google Scholar
Riley, RL and Kaufman, JE (1972). Effect of relative humidity on the inactivation of Serratia marcescens by ultraviolet radiation. Applied Microbiology 23: 1113–1120.Google Scholar
Rivers, T and Gates, F (1927). Ultra-violet light and vaccine virus. II. The effect of monochromatic ultra-violet light upon vaccine virus. Journal of Experimental Medicine 47: 45–49.Google Scholar
Rubin, A and Elmaraghy, G (1977). Studies on the toxicity of ammonia, nitrate and their mixture to guppy fry. Water Research 11: 927–935.Google Scholar
Sarasin, AR and Hanawalt, PC (1978). Carcinogens enhance survival of UV-irradiated simian virus 40 in treated monkey kidney cells: induction of a recovery pathway? Proceedings of the National Academy of Sciences of the United States of America 75: 346–350.CrossRefGoogle ScholarPubMed
Selma, MV, Allende, A, López-Gálvez, F, Conesa, MA and Gil, MI (2008). Disinfection potential of ozone, ultraviolet-C and their combination in wash water for the fresh-cut vegetable industry. Food Microbiology 25: 809–814.Google Scholar
Shen, C, Fang, S, Bergstrom, D and Blatchley, E (2005). (E)-5-[2-(Methoxycarbonyl)ethenyl]-cytidine as a chemical actinometer for germicidal UV radiation. Environmental Science and Technology 39: 3826–3832.Google Scholar
Shore, VG and Pardee, A (1956). Energy transfer in conjugated proteins and nucleic acids. Archives of Biochemistry and Biophysics 62: 355–368.Google Scholar
Smith, D and Hanawalt, P (1969). Repair replications of DNA in ultraviolet irradiated Mycoplasma laidlawii. Journal of Molecular Biology 46: 57–72.CrossRefGoogle ScholarPubMed
Srinivasan, V, Schnitzlein, W and Tripathy, D (2001). Fowlpox virus encodes a novel DNA repair enzyme, CPD-Photolyase, that restores infectivity of UV light-damaged virus. Journal of Virology 75: 1681–1688.Google Scholar
Stevens, C, Khan, VA, Lu, JY, Wilson, CL, Chalutz, E, Droby, S, Kabwe, MK, Haung, Z, Adeyeye, O, Pusey, LP and Tang, AYA (1999). Induced resistance of sweet potato to Fusarium root rot by UV-C hormesis. Crop Protection 18: 463–470.CrossRefGoogle Scholar
Stowe, R (2005). Radiometric methods for ultraviolet-process design and process monitoring-advanced radiometry. Radtech Report 4: 12–20.Google Scholar
Tang, J, Eames, I, Chan, P and Ridgway, G (2006). Factors involved in the aerosol transmission of infection and control of ventilation in healthcare premises. Journal of Hospital Infection 64: 100–114.CrossRefGoogle ScholarPubMed
Thurston-Enriquez, J, Haas, C, Jacangelo, J, Riley, K and Gerba, C (2003). Inactivation of feline calicivirus and adenovirus type 40 by UV radiation. Applied and Environmental Microbiology 69: 577–582.Google Scholar
Tseng, C and Li, C (2005). Inactivation of virus-containing aerosols by ultraviolet irradiation. Aerosol Science and Technology 39: 1136–1142.CrossRefGoogle Scholar
Van Heuvelen, A (1982). Physics: A General Introduction. Boston, MA: Little, Brown & Co.Google Scholar
VanOsdell, D and Foarde, K (2002). Defining the effectiveness of UV lamps in circulating air ductwork. ARTI 21-CR/610-40030-01.Google Scholar
Walker, C and Ko, G (2007). Effect of ultraviolet germicidal irradiation on viral aerosols. Environmental Science and Technology 41: 5460–5465.Google Scholar
Ward, M (1892). Experiments of the action of light on Bacillus anthracis. Proceedings of the Royal Society of London 52: 393–403.Google Scholar
Wells, W and Wells, M (1938). Measurement of sanitary ventilation. American Journal of Public Health 28: 343–350.Google Scholar
Wheeler, SM, Ingraham, HS, Hollaneder, A, Lill, ND, Gershon-Cohen, J Jr and Brown, EW (1945). Ultraviolet light control of airborne infections in a naval training center. American Journal of Medicine 35: 457–468.Google Scholar
Wolfe, R (1990). Ultraviolet disinfection of potable water: current technology and research needs. Environmental Science and Technology 24: 768–773.Google Scholar
Wong, S and Yuen, K (2006). Avian influenza infections in humans. Chest 129: 156–168.Google Scholar