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Human intestinal spirochetosis diagnosed with colonoscopy and analysis of partial 16S rDNA sequences of involved spirochetes

Published online by Cambridge University Press:  28 February 2007

Wolfgang Kraatz ,
Ulf Thunberg ,
Bertil Pettersson  and
Claes Fellström
Wolfgang Kraatz
Affiliation:
Department of Pathology, The University Hospital, Uppsala, Sweden
Ulf Thunberg
Affiliation:
Department of Oncology, The University Hospital, UppsalaSweden
Bertil Pettersson
Affiliation:
Department of Biotechnology, The Royal Institute of Technology, StockholmSweden
Claes Fellström*
Affiliation:
Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Uppsala, Sweden
*
*Department of Large Animal Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7018, SE-750 07 Uppsala, Sweden. E-mail: [email protected]
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Abstract

DNA was extracted from colonic biopsies of 33 patients with and three without evidence of intestinal spirochetosis (IS) in the large bowel. The biopsies were subjected to PCR. A pair of primers, generating a 207 bp fragment, were designed to detect specifically the 16S rDNA gene of Brachyspira. PCR products of the expected size were obtained from 33 samples with histologic evidence of IS. The PCR amplicons were used for sequencing. The sequences obtained were aligned to the corresponding 16S rRNA sequences of five type strains of Brachyspira. The sequences of 23 PCR products were 99–100% identical with the correspond-ing B. aalborgi type strain sequence. Two cases showed 99–100% sequence similarity with the type strain of B. pilosicoli P43/6/78. Six cases could not be referred to any of the known species of Brachyspira. Two PCR products gave incomplete sequences.

Type
Research Article
Information
Animal Health Research Reviews , Volume 2 , Issue 1 , June 2001 , pp. 111 - 116
Copyright
Copyright © CAB International 2001

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References

Barret, SP (1990). Intestinal spirochaetes in a Gulf Arab population. Epidemiology and Infection 104: 261–266.CrossRefGoogle Scholar
Cotton, DWK, Kirkham, N and Hicks, DA (1984). Rectal spirochaetosis. British Journal of Venereal Diseases 60: 106–109.Google ScholarPubMed
Crusioli, V and Busuttil, A (1981). Human intestinal spirochaetosis. Scandinavian Journal of Gastroenterology 70 (Suppl.): 177–179.Google Scholar
Diaz-Cano, SJ and Brady, SP (1997). DNA extraction from forma-lin-fixed, paraffin-embedded tissues: protein digestion as a limiting step for retrieval of high-quality DNA. Diagnostic and Molecular Pathology 6: 342–346.CrossRefGoogle ScholarPubMed
Douglas, JG and Crusioli, V (1981). Spirochaetosis: a remediable cause of diarrhoea and rectal bleeding. British Medical Journal 283: 1362.CrossRefGoogle ScholarPubMed
Duhamel, GE, Elder, RO, Muniappa, N, Mathiesen, MR, Wong, VJ and Tarara, RP (1997). Colonic spirochetal infection in nonhuman primates that were associated with Brachyspira aalborgi, Serpulina pilosicoli and unclassified flagellated bacteria. Clinical Infectious Diseases 25 (Suppl. 2): 186–188.CrossRefGoogle ScholarPubMed
Duhamel, GE, Trott, DJ, Muniappa, MR, Mathiesen, MR, Tarasuik, K, Lee, JI and Hampson, DJ (1998). Canine intestinal spirochetes consists of Serpulina pilosicoli and a newly identified group provisionally designated “Serpulina canis” sp. nov. Journal of Clinical Microbiology 36: 2264–2270.CrossRefGoogle Scholar
Escherich, T (1884). Klinisch therapeutische Beobachtungen aus der Cholera-Epidemie in Neapel. Aerztliche Intelligenzblatt 31: 561–564.Google Scholar
Fellström, C and Gunnarsson, A (1995). Phenotypical characteri-sation of intestinal spirochaetes isolated from pigs. Research in Veterinary Science 59: 1–4.CrossRefGoogle ScholarPubMed
Gad, A, Willén, R, Furugärd, K, Fors, B and Hradsky, M (1977). Intestinal spirochaetosis as a cause of long-standing diar-rhoea. Uppsala Journal of Medical Science 82: 49–54.CrossRefGoogle Scholar
Gear, EV and Dobbins, WO (1968). Rectal biopsy. Gastroenterology 55: 522–544.CrossRefGoogle ScholarPubMed
Gebbers, JO, Ferguson, DJP, Mason, C, Kelly, P and Jewell, DP (1987). Spirochaetosis of the human rectum associated with an intraepithelial mast cell and IgE plasma cell response. Gut 28: 588–593.CrossRefGoogle ScholarPubMed
Guccion, JG, Benator, DA, Zeller, J, Termanini, B and Saini, N (1995). Intestinal spirochetosis and acquired immunodefi-ciency syndrome: ultrastructural studies of two cases. Ultrastructural Pathology 19: 15–22.CrossRefGoogle Scholar
Harland, WA and Lee, FD (1967). Intestinal spirochaetosis. British Medical Journal 3:718–719.CrossRefGoogle ScholarPubMed
Hovind-Hougen, K, Birch-Andersen, A, Henrik-Nielsen, R, Orholm, M, Pedersen, JO, Teglbjærg, PS and Thayson, EH (1982). Intestinal spirochetosis: Morphological characterization of the spirochete Brachyspira aalborgi gen. nov., sp. nov. Journal of Clinical Microbiology 16: 1127–1136.CrossRefGoogle ScholarPubMed
Jones, MJ, Miller, JN and George, WL (1986). Microbiological and biochemical characterization of spirochaetes isolated from the faeces of homosexual males. Journal of Clinical Microbiology 24: 1071–1074.CrossRefGoogle ScholarPubMed
Kaplan, LR and Takeuchi, A (1979). Purulent rectal discharge associated with a nontreponemal spirochaete. Journal of the American Medical Association 241: 52–53.CrossRefGoogle Scholar
Käsbohrer, A, Gelderblom, HR, Arasteh, K, Heise, W, Grosse, G, L'age, M, Schönberg, A, Koch, MA and Pauli, G (1990). Intestinale spirochätose bei HIV-Infektion. Deutsche Medizinische Wochenschrift 115: 1499–1506.CrossRefGoogle Scholar
Koopman, MBH, Käsbohrer, A, Beckmann, G, van der Zeijst, BAM and Kusters, JG (1993). Genetic similarity of intestinal spirochaetes from humans and various animal species. Journal of Clinical Microbiology 31: 711–716.CrossRefGoogle ScholarPubMed
Kostman, JR, Patel, M, Catalano, E, Camacho, J, Hoffpauir, J and DiNubile, MJ (1995). Invasive colitis and hepatitis due to previously uncharacterized spirochetes in patients with advanced human immunodeficiency virus infection. Clinical and Infectious Diseases 21: 1159–1165.CrossRefGoogle ScholarPubMed
Kraaz, W, Pettersson, B, Thunberg, U, Engstrand, L and Fellström, C (2000). Brachyspira aalborgi infection diagnosed by culture and 16S ribosomal DNA sequencing using human colonic biopsy specimen. Journal of Clinical Microbiology 38: 3555–3560.CrossRefGoogle Scholar
Lee, JI and Hampson, DJ (1992). Intestinal spirochaetes colonizing Aborigines from communities in the remote north of Western Australia. Epidemiology and Infection 109: 133–141.Google ScholarPubMed
Lee, JI and Hampson, DJ (1994). Genetic characterisation of intestinal spirochaetes and their association with disease. Journal of Medical Microbiology 40: 365–371.CrossRefGoogle ScholarPubMed
Lee, FD, Kraszevski, A, Gordon, J, Howie, JGR, McSevene, D and Harland, WA (1971). Intestinal spirochaetosis. Gut 12: 126–133.CrossRefGoogle ScholarPubMed
Lindboe, CF, Tostrup, NE, Nersund, R and Rekkavik, G (1993). Human intestinal spirochaetosis in mid-Norway. A retrospective histopathological study with clinical correlations. APMIS 101: 858–864.CrossRefGoogle ScholarPubMed
Macfie, JWS (1916). The morphology of certain spirochaetes of man and other animals. Annals of Tropical Medicine and Parasitology 10: 305–343.CrossRefGoogle Scholar
Mikosza, ASJ, La, T, Brooke, J, Lindboe, C, Ward, PB, Heine, RG, Guccion, JG, Bastiaan de Boer, W and Hampson, DJ (1999). PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis. Journal of Clinical Microbiology 37: 2093–2098.CrossRefGoogle ScholarPubMed
Muniappa, N, Duhamel, GE, Mathiesen, MR and Bargar, TW (1996). Light microscopic and ultrastructural changes in the ceca of chicks inoculated with human and canine Serpulina pilosicoli . Veterinary Pathology 33:542–550.CrossRefGoogle ScholarPubMed
Nielsen, RH, Orholm, M, Pedersen, JO, Hovind-Hougen, K, Teglbjærg, PS and Thaysen, EH (1983). Colorectal spirochaetosis: clinical significance of the disease. Gastroenterology 85: 62–67.CrossRefGoogle Scholar
Padmanabhan, V, Dahlstrom, J, Maxwell, L, Kaye, G, Clarke, A and Barrett, PJ (1996). Invasive intestinal spirochetosis: A report of three cases. Pathology 28: 283–286.CrossRefGoogle ScholarPubMed
Parr, LW (1923). Intestinal spirochetes. Journal of Infectious Diseases 33: 369–383.CrossRefGoogle Scholar
Pettersson, B, Wang, M, Fellström, C, Uhlén, M, Molin, G, Jeppsson, B and Ahrné, S (2000). Phylogenetic evidence for novel and genetically different intestinal spirochetes resembling Brachyspira aalborgi in the mucosa of the human colon as revealed by 16S rDNA analysis. Applied and Systematic Microbiology. 23: 355–363.CrossRefGoogle ScholarPubMed
Sacco, RE, Trampel, DW and Wannemuehler, MJ (1997). Experimental infection of C3H mice with avian, porcine, or human isolates of Serpulina pilosicoli . Infection and Immunity 65:5349–5353.CrossRefGoogle ScholarPubMed
Trivett-Moore, NL, Gilbert, GL, Law, CLH, Trott, DJ and Hampson, DJ (1998). Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spiro-chetosis. Journal of Clinical Microbiology 36: 261–265.CrossRefGoogle Scholar
Trott, DJ and Hampson, DJ (1998). Evaluation of day-old spe-cific-pathogen-free chicks as an experimental model for pathogenicity testing of intestinal spirochete species. Journal of Comparative Pathology 118: 365–381.CrossRefGoogle Scholar
Trott, DJ, McLaren, AJ and Hampson, DJ (1995). Pathogenicity of human and porcine intestinal spirochetes in one-day-old specific-pathogen-free chicks: an animal model of intestinal spirochetosis. Infection and Immunity 63: 3705–3710.CrossRefGoogle ScholarPubMed
Trott, DJ, Huxtable, CR and Hampson, DJ (1996 a). Experimental infection of newly weaned pigs with human and porcine strains of Serpulina pilosicoli . Infection and Immunity 64: 4648–4654.CrossRefGoogle ScholarPubMed
Trott, DJ, Stanton, TB, Jensen, NS, Duhamel, GE, Johnson, JL and Hampson, DJ (1996 b). Serpulina pilosicoli sp. nov.: the agent of porcine intestinal spirochetosis. International Journal of Systematic Bacteriology 46:206–215.CrossRefGoogle ScholarPubMed
Trott, DJ, Mikosza, AS, Combs, BG, Oxberry, SL and Hampson, DJ (1998). Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the eastern Highlands of Papua New Guinea. International Journal of Systematic Bacteriology 48: 659–668.CrossRefGoogle ScholarPubMed
Willén, RB, Carlén, B, Cronstedt, J and Willén, H (1985). Intestinal spirochaetosis of the colon diagnosed with colono-ileoscopy and multiple biopsies. Endoscopy 17:86–88.CrossRefGoogle ScholarPubMed