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Fipa cattle in the southwestern highlands of Tanzania: molecular characterization

Published online by Cambridge University Press:  03 October 2012

P.L. Mwambene ,
A.M. Katule ,
S.W. Chenyambuga ,
Y. Plante  and
P.A.A. Mwakilembe
P.L. Mwambene*
Affiliation:
Livestock Research Centre Uyole, Directorate of Research, Training and Extension, Ministry of Livestock and Fisheries Development, PO Box 6191, Mbeya, Tanzania
A.M. Katule
Affiliation:
Department of Animal Science and Production, Faculty of Agriculture, Sokoine University of Agriculture, PO Box 3004, Morogoro, Tanzania
S.W. Chenyambuga
Affiliation:
Department of Animal Science and Production, Faculty of Agriculture, Sokoine University of Agriculture, PO Box 3004, Morogoro, Tanzania
Y. Plante
Affiliation:
Canadian Animal Genetic Resources Program, Agriculture and Agri-Food Canada, 107 Science Place Saskatoon, Saskatchewan, Canada
P.A.A. Mwakilembe
Affiliation:
Livestock Research Centre Uyole, Directorate of Research, Training and Extension, Ministry of Livestock and Fisheries Development, PO Box 6191, Mbeya, Tanzania
*
Correspondence to: P.L. Mwambene, Livestock Research Centre Uyole, Directorate of Research, Training and Extension, Ministry of Livestock and Fisheries Development, Mbeya, Tanzania. email: [email protected]
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Summary

This study aimed at characterising the genetic diversity of two Fipa cattle populations (Sumbawanga and Nkasi) of South-Western Tanzania, and establishing their genetic relationships with the other indigenous cattle strains (Tarime, Iringa red and Ankole) and Friesian cattle found in the area. The genetic diversity was analysed using 30 microsatellite markers. All the markers used were highly polymorphic. The Nkasi Fipa cattle exhibited the highest mean number of alleles (7.31) and mean genetic diversity (0.732) per locus, followed by Sumbawanga Fipa cattle with 7.10 mean number of alleles and 0.725 mean genetic diversity per locus, with the latter population having a very low mean inbreeding coefficient (FIS = 0.027). Three percent of the genetic diversity was due to differences among indigenous strains while the rest was due to differences among individuals within the strains. Small genetic distances (DA) were observed between Sumbawanga Fipa and Nkasi Fipa (0.032), Tarime (0.073), Iringa red (0.076) and Ankole cattle (0.086). As expected, the largest genetic distances were observed between the Friesian and all indigenous strains since this breed has a quite distinct genetic origin. In the assignment test, the proportion of animals from each group correctly assigned to their source population ranged from 55.3 percent (for Nkasi Fipa) to 100 percent (for Friesian). Despite the low genetic differentiation and genetic indistinctiveness of the Sumbawanga Fipa population from the other indigenous strains, its high genetic diversity, very low inbreeding coefficient and a threat emanating from population admixture with other indigenous strains underscore the importance of establishing appropriate conservation and management strategies for it.

Résumé

Cette étude visait à caractériser la diversité génétique de deux populations de bovins Fipa (Sumbawanga et Nkasi) en Tanzanie du Sud-Ouest, et à établir leurs relations génétiques avec les autres souches indigènes de bovins (Tarime, Iringa Red et Ankole) et avec les bovins Friesian présents dans la région. La diversité génétique a été analysée en utilisant 30 marqueurs microsatellites. Tous les marqueurs utilisés étaient hautement polymorphiques. Les bovins Fipa Nkasi présentaient le nombre moyen d'allèles (7,31) et la diversité génétique moyenne (0,732) par locus les plus élevés, suivis par les bovins Fipa Sumbawanga qui possédaient un nombre moyen d'allèles de 7,10, une diversité génétique moyenne par locus de 0,725 et un coefficient de consanguinité très faible (FIS = 0,027). Trois pour cent de la diversité génétique provenait des différences entre les souches indigènes tandis que le reste résultait des différences entre les animaux au sein des souches. Les distances génétiques (DA) étaient faibles entre les bovins Fipa Sumbawanga et Nkasi (0,032), Tarime (0.073), Iringa Red (0,076) et Ankole (0.086). Comme prévu, on a observé les distances génétiques les plus élevées entre toutes les souches indigènes et les bovins Friesian en raison des origines génétiques assez différentes de cette race. Lors du test d'attribution, la proportion d'animaux de chaque groupe qui ont été correctement assignés à leur population d'origine variait entre 55,3 pour cent (pour les Fipa Nkasi) et 100 pour cent (pour les Friesian). Malgré la faible différenciation génétique et le manque de traits génétiques distinctifs de la population de Fipa Sumbawanga par rapport aux autres souches indigènes, sa diversité génétique élevée, son coefficient de consanguinité très faible et la menace provenant du mélange génétique avec les autres souches indigènes mettent en évidence l'importance de planifier des stratégies adéquates pour sa conservation et sa gestion.

Resumen

Este estudio tuvo como objetivo caracterizar la diversidad genética de dos poblaciones bovinas de la raza Fipa (Sumbawanga y Nkasi) de la región suroccidental de Tanzania, y establecer sus relaciones genéticas con otras razas bovinas que se encuentran en la zona, tanto autóctonas (Tarime, roja Iringa y Ankole) como la Frisona. La diversidad genética se analizó utilizando 30 marcadores microsatélites. Todos los marcadores utilizados fueron altamente polimórficos. La población Nkasi Fipa presentó el mayor número medio de alelos (7,31) y la mayor diversidad genética media (0.732) por locus, seguida por la población Sumbawanga Fipa con un número medio de alelos de 7,10 y una diversidad genética media por locus de 0.725, mostrando esta última población un coeficiente promedio de consanguinidad muy bajo (FIS = 0,027). El 3% de la diversidad genética se debió a las diferencias entre razas autóctonas, mientras que el resto se debió a las diferencias entre individuos de la misma raza. Se observaron distancias genéticas (DA) pequeñas entre Sumbawanga Fipa y Nkasi Fipa (0.032), Tarime (0.073), roja Iringa (0.076) y Ankole (0,086). Como era de esperar, las mayores distancias genéticas se observaron entre la Frisona y todas las razas indígenas, ya que esta raza tiene un origen genético muy diferente. En la prueba de asignación, la proporción de animales de cada grupo asignado correctamente a su población de origen osciló entre 55,3 percent (para Nkasi Fipa) y el 100 percent (para la Frisona). A pesar de la baja diferenciación genética y de la indiferenciación genética de la población Sumbawanga Fipa respecto a las otras razas autóctonas, su alta diversidad genética, su muy bajo coeficiente de consanguinidad y la amenaza que representa el cruzamiento con otras razas indígenas resaltan la importancia de establecer estrategias adecuadas de conservación y de manejo de esta población.

Keywords

conservationgenetic diversityFipa populationindigenous strainsmicrosatellitesconservationdiversité génétiquepopulation de bovins Fipasouches indigènesmicrosatellitesconservacióndiversidad genéticapoblación Fiparazas indígenasmicrosatélites
Type
Research Article
Information
Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales , Volume 51 , December 2012 , pp. 31 - 43
Copyright
Copyright © Food and Agriculture Organization of the United Nations 2012

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