The sarus crane (Grus antigone) ranges across two continents and is the only species of crane (Gruidae) that breeds in India and Southeast Asia. Four subspecies, the Indian sarus (G. a. antigone), the eastern sarus (G. a. sharpii), the Australian sarus (G. a. gillae) and the extinct Philippine sarus (G. a. luzonica) were originally described through morphological, plumage, and/or geographical differences. The ranges of the Indian and eastern sarus converge in eastern India and Myanmar, but the Australian sarus has a disjunct Australian distribution. This study assesses population genetic structure of the current sarus populations utilising 13 DNA microsatellite loci. Population structure within this species was investigated utilising traditional FST and Bayesian clustering methods. While significant divergence was observed among populations when individuals were assigned to geographical populations, analyses based solely on individual genotypes demonstrated a clinal nature to the variation. The results of this study suggest that the Indian and eastern sarus cranes represent two major breeding groups within Asia and that the birds from Myanmar probably reside in an introgression zone between them. Lastly, because of genetic similarities shown to the eastern sarus, the results support recent theories contending that the Australian sarus was derived from mainland Asian birds.

Among tourist activities in the Alps, winter sports have a prominent role because of the large scale of changes they cause at the habitat and landscape level. We have analysed whether ski resorts lead to significant threats to the avian diversity in the coniferous forests of the western Italian Alps, by comparing the bird communities of plots located in (1) the forest interior, (2) forest at the edge of ski-runs and (3) forest at the edge of pastures (the latter two are anthropogenic elements of forest fragmentation). Ski-runs produce a negative edge effect in the study forests: plots at their edges present lower bird species richness and Shannon diversity than those located in the forest interior or at the edges of pastures. In particular, birds typical of ecotone habitats seem to favour forest plots set at the edge of pastures. Ski-run-edges are linear landscape features that create high contrast edges; conversely, vegetation structure is more complex at the edge of pastures, attracting a rich and diverse avifauna. In the study area, pastures tend to be abandoned whereas winter sport resorts are increasing in extent. Accordingly, there is a need for coordinated management and cooperation between sport- and land-management agencies, in order to preserve native biodiversity while simultaneously managing land for sport activities.

In Italy, pre-Alpine forests, once managed through coppice silviculture, are being converted to mature woodland, while land abandonment is causing woodland expansion and erosion of open habitats. Based on habitat-selection analyses, we predicted the impact of such changes on common buzzards (Buteo buteo), which depend on forested and open areas for nesting and foraging. Compared to availability, at a micro-scale buzzards selected nests higher above ground and on trees frequently covered by ivy. At the landscape-scale, buzzards avoided roads and conspecifics, while selecting rugged areas with high habitat heterogeneity, probably related to a varied food supply. Productivity was related to the availability of arid habitats, probably because of their richness in main prey species. Finally, population density was negatively related to the abundance of eagle owls (Bubo bubo), a potential predator of adults and nestlings, and positively related to the availability of woodland, a low predation-risk habitat rich in food and nest-sites. Therefore, buzzard settlement, density and productivity depended on the complex interplay of food availability, human persecution and predation risk. Thus, the current landscape changes would benefit buzzards by providing more nest-sites, but would be detrimental because of the lower productivity associated with the disappearance of dry open areas. Proposed conservation guidelines focus on conversion of coppice woodland to mature forests and active management of dry heath, a conservation sensitive habitat, through controlled burning.

Droughts induced by the 1997–1998 El Niño Southern Oscillation (ENSO) event sparked large-scale forest fires affecting millions of hectares on Borneo. We studied the effects of ENSO-induced disturbances on a riverine bird, the critically endangered white-shouldered ibis, Pseudibis davisoni, along the Mahakam River in East Kalimantan, Indonesian Borneo. Island-wide fire hotspots were more likely to occur near rivers and part of our study area was indeed affected by fire. Based on 25 boat surveys (2590 km) that yielded 91 records, we recorded significant changes in abundance and spatial distribution of this bird in our study area. Encounter rates were higher during the pre-ENSO (1992–1996) periods than the post-ENSO (1997–2000) ones and differed between seasons. No ibises were seen post-ENSO along river sections that were affected by the fires, whereas pre-ENSO encounter rates were 2.4 birds/102 km. Encounter rates along those sections that were not affected by the forest fires more than doubled from 2.0 birds/102 km pre-ENSO to 5.6 birds/102 km post-ENSO. This was most probably due to enhancement of displaced birds. In view of the permanent character of the change in spatial distribution, active protection of the remaining stretches of riverine forest is of the utmost importance for the survival of white-shouldered ibis.

Relocation of endangered species can be an effective conservation tool if it does not mix populations that represent significant intraspecific variation. The threatened Italian agile frog, Rana latastei, has small populations with low genetic diversity: translocation has been proposed to improve the likelihood of survival of populations. Using a common environment experiment and field surveys, we investigated whether there were differences in larval growth and developmental rate between foothill and lowland R. latastei populations, to evaluate if they are evolutionarily significant units. In nature, the colder climate of the foothills causes delayed metamorphosis. Conversely, in a common environment, larvae from foothill populations show faster growth and development. We did not find a significant egg-size related maternal effect or any differences in size at metamorphosis. We hypothesise that counter-gradient selection promoted fast growing phenotypes in a cold environment, where low temperatures slow down larval development. Foothill populations, despite being only a small geographical distance away from lowland populations, seem to be adapted to a colder climate and represent an evolutionarily significant unit. Different populations should, therefore, be managed independently, avoiding translocation. We suggest that evolutionary divergence between populations should be verified prior to planning relocation programmes, to prevent the risk of genetic homogenisation.

A set of priority sites for wildfowl conservation in Mexico was determined using contemporary count data (1991–2000) from the U.S. Fish & Wildlife Service mid-winter surveys. We used a complementarity approach implemented through linear integer programming that addresses particular conservation concerns for every species included in the analysis and large fluctuations in numbers through time. A set of 31 priority sites was identified, which held more than 69% of the mid-winter count total in Mexico during all surveyed years. Six sites were in the northern highlands, 12 in the central highlands, six on the Gulf of Mexico coast and seven on the upper Pacific coast. Twenty-two sites from the priority set have previously been identified as qualifying for designation as wetlands of international importance under the Ramsar Convention and 20 sites are classified as Important Areas for Bird Conservation in Mexico. The information presented here provides an accountable, spatially-explicit, numerical basis for ongoing conservation planning efforts in Mexico, which can be used to improve existing wildfowl conservation networks in the country and can also be useful for conservation planning exercises elsewhere.

Taxonomic bias has been documented in general science and conservation research publications. We examined whether taxonomic bias is similarly severe in actual conservation programmes as indicated by the focus of species reintroduction projects worldwide. We compiled a database of reintroduction projects worldwide, yielding a total of 699 species of plants and animals that are the focus of recent, current or planned reintroductions. Using IUCN (World Conservation Union) data for total numbers of known species worldwide, we found that vertebrate projects were over-represented with respect to their prevalence in nature. Within vertebrates, mammals and, to a lesser extent, birds, were over-represented, whereas fish were under-represented. This over-representation extended to two mammal orders, artiodactylids and carnivores, and to four bird orders, anseriforms, falconiforms, gruiforms and galliforms. For neither mammals nor birds was reintroduction project bias related to any differences between orders in vulnerability to threat. Bird species that are the focus of reintroduction efforts are more likely to be categorised as ‘Threatened’ than expected on the basis of the distribution of all known species over all threat categories, however, nearly half of all bird species being reintroduced are classified as ‘Least Concern’. The selection of candidates for reintroduction programmes is likely to consider national priorities, availability of funding and local community support, over global conservation status, While a focus on charismatic species may serve to garner public support for conservation efforts, it may also divert scarce conservation resources away from taxa more in need of attention.

The little bustard (Tetrax tetrax) has declined rapidly across European farmland landscapes due to agricultural intensification. In France, the number of breeding males in agricultural habitats has been reduced by 92% over the last 20 years as a result of decreases in insect abundance and nest destruction during harvesting. An age- and sex-structured stochastic metapopulation model was formulated for the remaining little bustard population in south-west France and, using actual estimates of demographic rates obtained after 1997, its extinction risk over the next 30 years was estimated to be ca. 0.48. Limited population reinforcement has thus appeared as a potential conservation strategy for this species in agricultural habitats, while agro-environmental actions have begun to have an effect on habitat quality at the landscape level. Different strategies for the reinforcement of fledglings, including the number and frequency of releases and the number of release localities in relation to four criteria for choosing the release points, were evaluated in terms of their effect in reducing the extinction risk of local populations and of the metapopulation. The reinforcement of 100 fledglings per year for 5 years and choosing the actual release points using the current abundance were found to be the optimal choices for reducing the estimated extinction risk of the remaining little bustard population in south-west France.

Hispaniola has been identified as a global priority for avian conservation. However, little quantitative information has been available to help guide optimal strategies for conservation action on the island. Here, the first broad-scale look has been assembled of the distribution of species of conservation concern among protected areas in Haiti and the Dominican Republic and their occurrences have been analysed to determine where conservation activities might be focused to serve avian conservation interests. An iterative, heuristic complementarity approach was used, such that the most highly ranked reserve is that which protects the greatest number of species of conservation concern and subsequently ranked reserves are those that add the most species of conservation concern that are not included in the first reserve. Parks are prioritised by the presence/absence of species of concern and prioritised a second time with individual species first being weighted by species-specific extinction risks and then by uniqueness in terms of endemism at the island or regional level. Parks of highest importance are the Sierra de Bahoruco National Park and Jaragua National Park, but the importance of other protected areas to avian conservation is also documented and discussed.

Substantial genetic variation is hypothesised to be necessary for the long-term survival of species. Therefore, a major aim in conservation is to maintain and restore variation in small and endangered populations. However, in most populations the amount of genetic variation and, thus, the potential threats posed by limited variation are unknown. In the present study, we assess genetic variation, both at 10 microsatellite loci and at the major histocompatibility complex (MHC), in three closely related Acrocephalus warbler species with contrasting demographies. We found that the recently bottlenecked, island endemic, Seychelles warbler (A. sechellensis; SW) has substantially reduced microsatellite and MHC diversity compared to the widespread great reed warbler (A. arundinaceus; GRW). In contrast, another endangered species with a small breeding range, the Basra reed warbler (A. griseldis; BRW), harboured as much variation as did the GRW. This suggests that significant genetic variation and, therefore, adaptive potential, remains in the BRW – a situation that should hold as long as its habitat and numbers are maintained. Our study is one of the first to assess genetic variation at both ‘non-critical’ microsatellite markers and ‘critical’ MHC loci within endangered species. The two types of loci provided a similar picture of the genetic variation in the species we studied, but this has not been the case in studies of some other species. Using a combination of specific functional loci and genome-wide random markers appears to be the best way to assess the threat posed by reduced genetic diversity.

Large carnivores have declined worldwide, largely through conflict with people. Here, we quantify the impact of lethal control, associated with livestock depredation, on a population of African lions (Panthera leo) living outside protected areas. Farmers shot lions only in response to livestock attacks. Nevertheless, adult mortality was high and a simple model predicted that the population was marginally stable or slowly declining. Mortality was four times higher among lions radio-collared in association with attacks on livestock, than among lions with no known history of stock killing, suggesting that some animals were habitual stock killers. Known stock killers also experienced lower reproductive success; hence there was strong artificial selection against stock-killing behaviour. In addition, mortality was higher among lions whose home ranges overlapped a property where non-traditional livestock husbandry was associated with chronic depredation by lions. This 180 km2 ranch acted as a sink that directly affected lions over more than 2000 km2 and may have undermined the viability of the study population. Our results suggest that sustainable coexistence of lions and people demands livestock husbandry that effectively deters predators from acquiring stock-killing behaviour, but that lethal control may play an important role in avoiding the spread of such behaviours through the population.

In Asia, subtropical tall grasslands and their associated fauna are mostly confined to the floodplains of the large rivers draining the Himalayan range. In recent decades, cultivation and hydroelectric dam construction have severely reduced their spatial abundance. During 1998–2000, we conducted a detailed study of the habitat use of hog deer, Axis porcinus, an important prey species of tiger, Panthera tigris, in the floodplains of the large Karnali river in Royal Bardia National Park, Nepal. Strong preference for tall grass and avoidance of the successional stages following riparian forests throughout the year was revealed by analyses of tracking data from 18 radio-collared deer and from density estimates from block counts (n=46) in different habitat types. Continuously strong selection for tall grass despite the reduced availability of this habitat type during the monsoonal flooding indicated an insignificant effect of density-dependent factors in habitat selection. Reduced availability of cover following traditional cutting and burning of the tall grasslands by local villagers did not produce changes in habitat use or an increase in daily movements, indicating an absence of trade-offs between habitat preference for food and for predator avoidance. By regulating fluvial action, hydroelectric power development will lead to the loss of tall grasslands due to accelerated secondary succession, which will therefore adversely affect the viability of local populations.

The black and white ruffed lemur, Varecia variegata, which is endemic to Madagascar, is under the threat of extinction due to the loss and fragmentation of its natural habitat, a consequence of deforestation. Behavioural researchers studying a population of V. variegata inhabiting the Manombo Special Reserve, noted that over a 4-year period, none of the family groups within the reserve produced offspring while those in neighbouring populations had been reproductively successful. Inbreeding depression was postulated as the trigger for this occurrence. However, another explanation exists. A cyclone, striking the region in 1997, destroyed a large percentage of the mature fruit-producing trees that V. variegata relied upon as a food source, which is likely to have produced a nutritional deficit. A panel of 25 simple sequence repeat (SSR) markers was utilised to assess the genetic diversity within four V. variegata populations, including individuals from Manombo and three other populations in southeastern Madagascar. The data revealed that the level of genetic diversity apparent within the Manombo population is comparable to the other groups studied. Therefore, it seems probable that genetic factors are not responsible for the recent lack of reproductive success on the part of the V. variegata at Manombo Special Reserve.