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Binding of Aliphatic Dihydroxy Compounds to Collagen with X-ray Diffraction Evidence for the Vander Waals Bond

Published online by Cambridge University Press:  06 March 2019

E. H. Shaw Jr.
E. H. Shaw Jr.*
Affiliation:
University of South Dakota, Vermillion, South Dakota 57069
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Abstract

Previous work presented by the author at these conferences has been summarized by G.N. Ramachandran in “Treatise on Collagen I , 177-8”. Epitactic crystallization of amides and diols in the collagen lattice of formalinized rat tail tendon, with equatorial expansion of the collagen lattice, was used to visualize the core diameter of each coil in the triple spiral at 7A and the vertical repeat of the hydrogen-bonding unit at 5.2A. Expansion of the non - formalinized collagen lattice has been observed as high as 20.1A when 1, 4-trans-(bisamino-methyl) - cyclo-hexane was inserted between the hexagonal array of protein spirals in the collagen lattice, the length of the inserted molecule being 13A. With dimensions based on Fisher - Hirschfelder - Taylor atomic models, with maximum extension of the dihydroxy compounds, the a. parameter of the expanded collagen would be expected to increase from 16.5A in the case of glycol to 20.6A in the case of 1, 5 - pentanediol, but actually the parameter decreases from 18.3A in the case of glycol to 16.2A in the case of 1, 5 - pentanediol. The larger figure in the case of glycol is explained by its hydrogen bonded dimerization. 1,3 propanediol fits in to the collagen lattice as its extended form. In the case of the other compounds, the chains are folded and fit together with Vander Waals bonding, and produce progressively smaller parameters for the a axis in collagen. In this nonaqueous equilibrium the non - formalinized collagen core diameter averages 7.7A ±0.2 and the spacing of hydrogen bond contacts along the collagen fiber axis is 4.8 ±0.2A.

Type
Research Article
Information
Advances in X-Ray Analysis , Volume 14: Nineteenth Annual Conference on Applications of X-ray Analysis, August 5-7, 1970 , 1970 , pp. 268 - 274
Copyright
Copyright © International Centre for Diffraction Data 1970

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References

Ramachandran, G.N., “Treatise on Collagen, Vol. I. Chemistry of Collagen”, Academic Press, N.Y. 1967 pp. 177-8.Google Scholar
Shaw, B.H., Jr . and Schulte, B., “The Collagen Lattice in 1,2- Propanediol and its Expansion by Hydrogen-bonding Materials”, Abstracts 6th International Congress of Biochemistry, New York, 1964, p. 180.Google Scholar
Shaw, Edwin H. Jr., “Oriented crystallization of Phloroglucinol in Collagen with Modification of the Collagen Lattice ”, Abstracts, Third International Biophysics Congress of the International Union of Pure and Applied Biophysics, p. 197, Cambridge, Mass. 1969.Google Scholar
Ramachandran, G.N. and Sasisekharan, V., “Refinement of the structure of Collagen”, Biocliim. Biophys. Acta, 109, 314 (1965).Google Scholar
Shaw, E.H. Jr., “Oriented crystallization of Amides on Collagen with Modification of the Collagen Lattice”, in Mueller, W.M., Mallett, G.R. and Fay, M.J., Editor, Advances in X-Ray Analysis, Vol. 7, p. 252-5, (1964).Google Scholar