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Abundance of beige and brown adipocyte markers in different adipose depots of cattle at 26 months of age

Published online by Cambridge University Press:  03 October 2017

K. Komolka ,
E. Albrecht ,
T. Gotoh  and
S. Maak
K. Komolka
Affiliation:
Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
E. Albrecht
Affiliation:
Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
T. Gotoh
Affiliation:
Kuju Agricultural Research Center, Kyushu University, Oita 819-0395, Japan
S. Maak*
Affiliation:
Institute for Muscle Biology and Growth, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany
*
†E-mail: [email protected]
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Abstract

Activity of brown and beige adipocytes could contribute to breed differences in fat deposition. Therefore, we compared the abundance of markers for adipocyte types in steers from three cattle breeds differing in fat deposition (Japanese Black, Holstein, Charolais). Markers for white (leptin (LEP)), beige (transmembrane protein 26 (TMEM26), uncoupling protein 1 (UCP1)), and brown adipocytes (Zic family member 1 (ZIC1), UCP1) were analysed by quantitative reverse transcription PCR in subcutaneous fat (SCF), intramuscular fat (IMF), intermuscular fat (IRMF), perirenal fat (PF) and visceral fat (VF). LEP messenger RNA (mRNA) was less abundant in VF compared with other depots (P<0.05). TMEM26 was weakly but evenly expressed in all depots in all animals, whereas UCP1 mRNA showed higher individual variation in some depots. ZIC1 was not detectable in VF and PF but abundant in SCF, IMF and even more abundant in IRMF (P<0.05). No significant breed differences were detected. Using antibodies against UCP1, TMEM26 and ZIC1, we demonstrated that fat depots of 26-month-old cattle still comprise different adipocyte types. However, our results did not support the hypothesis that higher energy expenditure associated with higher abundance or activity of beige or brown adipocytes contributed to differences in fat deposition.

Keywords

cattlebrown adipocytebeige adipocytegene expressionhistochemistry
Type
Full Paper
Information
Advances in Animal Biosciences , Volume 8 , Supplement s1: Improving the quality and sustainability of beef production , October 2017 , pp. s38 - s41
Copyright
© The Animal Consortium 2017 

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References

Albrecht, E, Gotoh, T, Ebara, F, Xu, JX, Viergutz, T, Nürnberg, G, Maak, S and Wegner, J 2011. Cellular conditions for intramuscular fat deposition in Japanese Black and Holstein steers. Meat Science 89, 13–20.Google Scholar
Asano, H, Yamada, T, Hashimoto, O, Umemoto, T, Sato, R, Ohwatari, S, Kanamori, Y, Terachi, T, Funaba, M and Matsui, T 2013. Diet-induced changes in Ucp1 expression in bovine adipose tissues. General and Comparative Endocrinology 184, 87–92.Google Scholar
Fisher, FM, Kleiner, S, Douris, N, Fox, EC, Mepani, RJ, Verdeguer, F, Wu, J, Kharitonenkov, A, Flier, JS, Maratos-Flier, E and Spiegelman, BM 2012. FGF21 regulates PGC-1a and browning of white adipose tissues in adaptive thermogenesis. Genes and Development 26, 271–281.Google Scholar
Kanamori, Y, Yamada, T, Asano, H, Kida, R, Qiao, Y, Abd Eldaim, MA, Tomonaga, S, Matsui, T and Funaba, M 2014. Effects of vitamin a status on expression of ucp1 and brown/beige adipocyte-related genes in white adipose tissues of beef cattle. The Journal of Veterinary Medical Science 76, 1261–1265.Google Scholar
Komolka, K, Albrecht, E, Schering, L, Brenmoehl, J, Hoeflich, A and Maak, S 2014. Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle? PLoS One 9, e88060.Google Scholar
Pfaffl, MW, Horgan, GW and Dempfle, L 2001. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 30, e36.Google Scholar
SAS/STAT user’s guide, v. 9.2. Cary, NC, USA: SAS Institute, Inc; 2011.Google Scholar
Walden, TB, Hansen, IR, Timmons, JA, Cannon, B and Nedergaard, J 2012. Recruited vs. no recruited molecular signatures of brown, ‘brite’, and white adipose tissues. American Journal of Physiology – Endocrinology and Metabolism 302, E19–E31.Google Scholar